Quantitative Trait Loci and Candidate Genes Affecting Fatty Acid Composition in Cattle and Pig

Dyah Maharani,조철훈,전진태,이준헌 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.3

Investigations into fatty acid composition in meats are becoming more important due to consumer demand for high quality healthy food. Marker-assisted selection has been applied to livestock to improve meat quality by directly selecting animals for favorable alleles that affect economic traits. Quantitative trait loci affecting fatty acid composition in cattle and pigs were investigated, and five candidate genes (ACACA, FASN, SCD, FABPs, and SREBP-1) were significantly associated with fatty acid composition. The information presented here should provide valuable guidelines to detect causative mutations affecting fatty acid composition in cattle and pigs.

Association of FASN and SCD genes with fatty acid composition in broilers

Dyah Maharani,Dong-Won Seo,Nu-Ri Choi,Shil Jin,Muhammad Cahyadi,Cheorun Jo,Jun-Heon Lee 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.3

Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

Investigation of SNPs in FABP3 and FABP4 Genes and Their Possible Relationships with Fatty Acid Composition in Broiler

Dyah Maharani,Hee Bok Park,Yeonkook Jung,Samooel Jung,Cheorun Jo,Jun-Heon Lee 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.3

There is limited information of the genetic effect for fatty acid composition in chicken meat. This study assessed the association of FABP3 and FABP4 genes affecting fatty acid composition in broilers. Two single nucleotide polymorphisms (SNPs) were detected in FABP3 gene and five SNPs were identified in FABP4 gene. The SNPs located in intron 1 and exon 1 of FABP3 and FABP4, respectively, were used for genotyping using PCR-RFLP method. The SNPg.285C >T in FABP4 showed suggestive association with high arachidonic acid (C20 : 4) in CT genotypes (P = 0.068). However, the SNP g.508C > T in FABP3 showed no significant associations with fatty acid composition. These results are the first report to investigate the SNPs in FABP3 and FABP4 genes and their associations with fatty acid composition, although we only found the possible association of FABP4 SNP with fatty acid composition. These results should provide valuable information for further investigation of the genes affecting fatty acid composition in chicken.

Quantitative Trait Loci and Candidate Genes Affecting Fatty Acid Composition in Cattle and Pig

Maharani, Dyah,Jo, Cheo-Run,Jeon, Jin-Tae,Lee, Jun-Heon Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.3

Investigations into fatty acid composition in meats are becoming more important due to consumer demand for high quality healthy food. Marker-assisted selection has been applied to livestock to improve meat quality by directly selecting animals for favorable alleles that affect economic traits. Quantitative trait loci affecting fatty acid composition in cattle and pigs were investigated, and five candidate genes (ACACA, FASN, SCD, FABPs, and SREBP-1) were significantly associated with fatty acid composition. The information presented here should provide valuable guidelines to detect causative mutations affecting fatty acid composition in cattle and pigs.

Association of FASN and SCD genes with fatty acid composition in broilers

Maharani, Dyah,Seo, Dong-Won,Choi, Nu-Ri,Jin, Shil,Cahyadi, Muhammad,Jo, Cheorun,Lee, Jun-Heon Institute of Agricultural Science 2013 Korean Journal of Agricultural Science Vol.40 No.3

Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

Evaluation of Three Candidate Genes Affecting Fatty Acid Composition in Pigs

Maharani, Dyah,Jung, Yeon-kuk,Jo, Cheorun,Jung, Woo-Young,Nam, Ki-Chang,Seo, Kang-Seok,Lee, Seung-Hwan,Lee, Jun-Heon Korean Society for Food Science of Animal Resource 2012 한국축산식품학회지 Vol.32 No.1

The association of three candidate genes, fatty acid synthase (FASN), microsomal triglyceride transfer protein (MTTP) and fatty acid binding protein 3 (FABP3), with fatty acid (FA) composition in Duroc pigs was investigated. Identified single nucleotide polymorphisms (SNPs) were used for polymerase chain reaction-restriction fragment length polymorphism genotyping. The c.265C>T SNP of FASN gene was significantly associated with high levels of palmitoleic acid (C16:1) (p<0.05), oleic acid (C18:1) (p<0.01), and mono-unsaturated fatty acid (MUFA) (p<0.01), but low levels of linoleic acid (C18:2) (p<0.01), alpha linolenic acid (C18:3) (p<0.05), and poly-unsaturated fatty acid (PUFA) (p<0.01) in animals having the CT genotype. The c.2573T>C SNP in the MTTP gene had a significant effect only in elevating the level of palmitoleic acid (C16:1) (p<0.05) in heterozygote animals. The polymorphism in FABP3 showed no significant effects on any fatty acid composition traits. These results suggest that the identified SNPs in the FASN and MTTP genes can be useful markers for selecting Duroc pigs having desirable healthy fatty acid composition.

Phenotypic characterization of local female duck populations in Indonesia

Dyah Maharani,Dwi N.H. Hariyono,Daniel D.I. Putra,이준헌,Jafendi H.P. Sidadolog 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.4

Information on phenotypic traits is a prerequisite in animal genetic resource conservation andimprovement. Phenotypic characteristics of 180 Indonesian female ducks collected from six populations(Alabio, Bayang, Magelang, Pegagan, Pitalah, and Rambon ducks) were studied. Data on qualitative traitswere analyzed with descriptive statistics (percentages), while one-way analysis of variance was used toanalyze the effect of populations on quantitative traits observed. The results revealed that the predominantplumage colors of the neck, breast, abdomen, back, secondary wings, primary wings, and thighwere brown (49.74%), brown (34.03%), brown (31.94%), brown (50.26%), dark brown (25.13%), light brown(35.08%), and brown (31.94%), respectively. The dominant bill and web color were black (77.49%) anddark brown (48.17), respectively. Variations were also found in quantitative morphological traits. Theprincipal component analysis showed two to four principal components extracted from the factoranalysis with varimax rotation, which accounted for 57.33%, 66.26%, 64.43%, 54.66%, and 77.44% of thetotal variance in the original variables for Alabio, Magelang, Pegagan, Pitalah, and Rambon ducks,respectively. Generally, the first factor in each population had high loadings on beak width. The observedphenotypic diversity in Indonesian local ducks could be useful in designing breeding programs andselection.

Haplotype Block Pattern and Linkage Disequilibrium Analysis of CAST Gene Polymorphisms in Boerka Goatt

Dyah Maharani,Simon P. Ginting,Simon Elieser,Andi Tarigan,I Gede Saputra Budisatria,Antonius,Dwi Nur Happy Hariyono,Aprilianna Putri Zahara Nafsina Luvita Sari 한국동물유전육종학회 2021 한국동물유전육종학회지 Vol.5 No.1

Calpastatin (CAST) gene has been considered as a candidate gene for meat quality traits in livestock animals. In this study, single nucleotide polymorphisms (SNPs) of the CAST gene were evaluated for determination of haplotype block pattern and linkage disequilibrium in 21 Boer × Kacang (Boerka) crossbred goats. Five SNPs: g.146C>A (SNP1), g.224A>G (SNP2), g.281G>A (SNP3), g.373C>T (SNP4) and g.431G>A (SNP5) were found in CAST gene (620 bp) using direct sequencing. Based on these SNPs, four haplotype block patterns (A, B, C and D) with different SNPs combination were performed. All blocks had high D’prime equal to 1.0. The highest LOD (1.75), r2 (1.00) and haplotype frequency (97.6%) were observed in block A (SNP1&2, SNP1&5 and SNP2&5). The lowest LOD (0.01) and r2 (0.0010) were found in block A (SNP1&4, SNP2&4 and SNP4&5). Block B, C and D had only 3, 2 and 1 SNPs combination, respectively, with a medium haplotype frequency (57.1%) each. Our study demonstrated that the SNPs and their haplotype combinations in the CAST gene could be useful for association studies with meat quality traits.

Evaluation of Three Candidate Genes Affecting Fatty Acid Composition in Pigs

Dyah Maharani,Yeonkuk Jung,Cheorun Jo,Woo-Young Jung,남기창,서강석,Seung Hwan Lee,이준헌 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.1

The association of three candidate genes, fatty acid synthase (FASN), microsomal triglyceride transfer protein (MTTP)and fatty acid binding protein 3 (FABP3), with fatty acid (FA) composition in Duroc pigs was investigated. Identified single nucleotide polymorphisms (SNPs) were used for polymerase chain reaction-restriction fragment length polymorphism genotyping. The c.265C>T SNP of FASN gene was significantly associated with high levels of palmitoleic acid (C16:1)(p<0.05), oleic acid (C18:1) (p<0.01), and mono-unsaturated fatty acid (MUFA) (p<0.01), but low levels of linoleic acid (C18:2) (p<0.01), alpha linolenic acid (C18:3) (p<0.05), and poly-unsaturated fatty acid (PUFA) (p<0.01) in animals having the CT genotype. The c.2573T>C SNP in the MTTP gene had a significant effect only in elevating the level of palmitoleic acid (C16:1) (p<0.05) in heterozygote animals. The polymorphism in FABP3 showed no significant effects on any fatty acid composition traits. These results suggest that the identified SNPs in the FASN and MTTP genes can be useful markers for selecting Duroc pigs having desirable healthy fatty acid composition.

Investigation of SNPs in FABP3 and FABP4 Genes and Their Possible Relationships with Fatty Acid Composition in Broiler

Maharani, Dyah,Park, Hee-Bok,Jung, Yeon-Kook,Jung, Samooel,Jo, Cheo-Run,Lee, Jun-Heon The Korean Society of Poultry Science 2011 韓國家禽學會誌 Vol.38 No.3

There is limited information of the genetic effect for fatty acid composition in chicken meat. This study assessed the association of FABP3 and FABP4 genes affecting fatty acid composition in broilers. Two single nucleotide polymorphisms (SNPs) were detected in FABP3 gene and five SNPs were identified in FABP4 gene. The SNPs located in intron 1 and exon 1 of FABP3 and FABP4, respectively, were used for genotyping using PCR-RFLP method. The SNPg.285C >T in FABP4 showed suggestive association with high arachidonic acid (C20 : 4) in CT genotypes (P = 0.068). However, the SNP g.508C > T in FABP3 showed no significant associations with fatty acid composition. These results are the first report to investigate the SNPs in FABP3 and FABP4 genes and their associations with fatty acid composition, although we only found the possible association of FABP4 SNP with fatty acid composition. These results should provide valuable information for further investigation of the genes affecting fatty acid composition in chicken.