家兎眼의 Acid Phosphatase의 정제 및 분자량 측정

노복자,석귀덕 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

Acid phosphatase was purified from rabbit eyes homogeneously. The homogeneity of the preparation was checked by means of SDS-polyacrylamide gel electrophoresis and densi-tometer trace. The isolation scheme involved a crude extract, fractionation with ammonium sulfate, extraction at PH 4.0, and chromatography on DEAE-cellulose. The end product represent about a 10.2-fold purification, and about a 19.1% recovery. The apparent molec-ular weight was estimated to be 48,000 by electrophoresis in polyacrylamide gel containing sodium dodecyl sulfate according to the method of Weber and Osborn.

「ケンチャナヨ」の機能の諸相

朴福德 中央大學校 日本硏究所 2004 日本 硏究 Vol.0 No.19

時變磁界 測定用 센서에 관한 硏究

이복희,백용현,박형기,정승수,전덕규 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

Synthesis and Characterization of Pr(Ⅲ)-,Sm(Ⅲ)-,Gd(Ⅲ)-,Dy(Ⅲ)-[18]HAM Complexes

정덕상,박신복,한충훈,변종철 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.1

Lanthanide(III) and mercury(II) complexes of the 18-membered hexaaza Schiff base macrocycle 2,11,17,26,31,32-hexaazapentacyclo[26,2.1.1??,O??,O??] dotriaconta-1(31), 2,4,6,8,10,12,14,16(32) ,17,19,21,23,25,27,29-hexadecane([18]HAM) ahve been synthesized by the metal template condensationof phthalic dicarboxaldehyde and 2,6-diaminopyridine. Discrete mononuclear complexes [Pr([18]HAM)(NO₃)(OH)]NO₃·2H₂O,[Sm([18]HAM)(NO₃)(H₂O)(OH)]NO₃·2H₂O, [Gd([18]HAM)(NO₃)(H₂O)(OH)]NO₃·3H₂O, [Dy([18])HAM)(NO₃)₂(H₂O)₂]NO₃·2H₂O, and [Hg([18])HAM)(H₂O)₂(ClO₄)]ClO₄· ?H₂O, have been synthesized by using the respective hydrated lanthanide(Ⅲ) nitrate and mercury(Ⅱ) perchlorate as the template. The lanthanide(Ⅲ)-marcrocyclic complexes (lanthanide(Ⅲ)-[18]HAM) was decomposed by c-HClO? forming [18] HAM and liberating the lanthanide(Ⅲ) ion. Co(Ⅱ)- Ni(Ⅱ)-Cu(Ⅱ)-complexes of acyclic, potentially tridentate compartmental Schiff bases ([9]acy) complexes containing d-metal ion Co(Ⅱ), Ni(Ⅱ), and Cu(Ⅱ) ion have been prepared by using metal template condensation of phthalic dicarboxaldehytde and 2,6-diaminopyridine. The complexes and macrocycle were characterized by elemental analysis, fast atom bombardment mass spectrometry, infrared spectroscopy, and molar conductivity. The template potential of the lanthanide(Ⅲ) cations of various sites in the assembly of [18]HAM is due to the flexibility of the macrocycle to adapt to the geometrical requirements of the metal ions and to the steric demands of the exocyclic ligands

양식넙치(Paralichthys olivaceus)의 생리조건에 미치는 연속적인 수온 급강하의 영향

강덕영,박명롱,이복규,장영진 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

Physiological responses of cultured olive flounder (Paralichthys olivaceus) on lowering seawater temperature sharply and continuously were studied with 4 experiments of temperature changes (Exp.Ⅰ∼Ⅳ). In Exp.Ⅰ, the temperature was decreased from 18℃ to 9℃ by the rate of 1℃/hr, thereafter back to the initial temperature after 5 days. With the same conditions of temperature rate and 5 days interval, the temperature changes for Exp. Ⅱ, Ⅲ and Ⅳ were 20℃ to 17℃, 23℃ to 14℃ and 23℃ to 17℃, respectively. Serum cortisol and glucose were measured during whole experiments. Hematocrit(Ht), hemoglobin(Hb), red blood cell(RBC), and mean corpuscular hemoglobin concentration(MCHC) were measured in the Exp.Ⅰ, and osmolality, electrolytes(Na^+, Cl^-, Ka^+, Ca^2+), total protein, aspartate aminotransferase(AST) and alanine aminotransferase(ALT) of serum, in Exp.Ⅱ∼Ⅳ. Serum cortisol levels were significantly increased by the lowering temperature sharply during whole experiments, while serum glucose levels were increased only in Exp.Ⅲ and Ⅳ. Ht, RBC and Hb were decreased as the water temperature was lowered, but MCHC was increased. The serum osmolality was reduced and the unstable changes of electrolytes were shown by the changes of seawater temperature. No significant changes in total protein, ALT and AST activity were observed.

자궁경부암에서 H-ras의 재배열과 v-mos의 증폭

김재형,함덕상,고복현,김대광 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Uterine cervical cancer is the most common cancer of woman in Korea. To understand which oncogenes are involved in squamous cell carcinomas of the uterine cervix, author examined nine human tissues of squamous cell carcinomas of the uterine cervix using Southern blotting techniques for five known oncogenes (H-ras, v-mos, erbB2, c-myc, K-ras). One of the nine tumors demonstrated a rearrangement of the H-ras oncogene. And amplification of v-mos was found in three tumors. The other oncogenes(erbB2, c-myc and K-ras) were the same pattern with the control. It is presumed that rearrangement of H-ras and amplifications of v-mos may be concerned to oncogenesis of squamous cell carcinomas of uterine cervix.

포항산 천연 제올라이트와 합성 제올라이트에 의한 금속 이온의 제거

박상윤,장세복,김덕수,김양 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

국내 경상북도 포항시 남구에서 채취한 4종의 천연 제올라이트를 화학성분 분석, X-선 회절, 열시차분석 실험, 열중량분석 실험으로 분석하였다. 이들 제올라이트의 주성분은 석영, mordenite, heulandite가 함유된 clinoptilolite이었다. NaOH, Ca(OH)₂, HCI 용액으로 처리한 천연 제올라이트의 성질은 X-선 회절법과 열시차분석 실험법으로 연구하였다. 천연 제올라이트와 화학적으로 처리한 제올라이트, 합성 제올라이트를 이용하여 Cu²+, Cd²+, Hg²+, Pb²+, Cr6+등의 중금속 이온과 알칼리 금속 K- 이온의 제거능력을 비교하였고, 중금속 이온 용액의 pH값과 초기농도, 중금속 이온을 제거하는데 소요되는 반응 시간의 효과 등을 비교하였다. 천연 제올라이트에 Ca²+, Pb²+, and Cu²+ 이온용액을 각각 5ppm첨가한 결과 제거율이 94%, 95%, 97%까지 증가되었다. 또한 0.5N NaOH와 0.5 N HCI 용액으로 처리한 매석산 천연 제올라이트를 이용하여 Hg²+ 이온용액을 5ppm 첨가한 결과 제거율이 약 80% 정도였다. 매석산 천연 제올라이트와 합성 제올라이트 A,X 및 Y가 Hg²+ 이온의 최대 제거율을 나타내었다. The four natural zeolites collected in Pohang, Kyungsang buk-do, Korea, were analyzed by means of wet chemical methods, X-ray diffraction, DTA, and TGA. The results indicate that the primary species of those zeolites are clinoptilolite mixed with quartz, mordenite, and heulandite. These zeolites were treated with NaOH, Ca(OH)₂, and HCI solution and their differences were also studied by X-ray diffraction method and differential thermal analysis method. The capabilities of removing heavy metal ions, such as Cu²+, Cd²+, Hg²+, Pb²+ and Cr6+, and alkaline ion, K+ ions with original zeolites, chemically treated zeolites, and synthetic zeolites were compared. The effect of pH value of solution and initial concentration of heavy metal ions, and the effect of reaction time in removing heavy metal ions were ions were studied. The experimental results showed that up to 94%, 95%, and 97% of Ca²+, Pb²+, and Cu²+ ions could be removed, respectively, out of 5 ppm of untreated natural zeolites. About 80% of the Hg²+ ions could be also removed out of 5 ppm Hg²+ ions solution with Maesuk mt. natural zeolite which was treated with 0.5N NaOH and 0.5 N HCI solution. It was found that Maesuk mt. natrual zeolite was most efficient in removing Hg²+ ions compared with the other natural zeolites studied in this work and synthetic zeolite A, X and Y.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

아황산가스가 집파리의 산란시기와 우화율에 미치는 영향

이해풍,이복임,최덕일 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

When Musca domestica L. was exposed to the different levels of SO_2(0.3, 0.4, 1.5 ppm) in an air pollutant fumigation chamber, in the case of 0.4 ppm exposed, their oviposition rate significantly decreased with the increasing exposure period at 0.4 ppm and their oviposition was also delayed. The number of pupae from the house fly exposed to SO_2 distinctly decreased when compared with control group and this trend was more pronounced at F_2 than at F_1 generation. The emergence of pupae from the cohorts exposed to SO_2 showed a distinctly decreasing trend with the increase in exposure period and in the concentration of SO_2.

한국인 알코올 의존 환자에서 알코올 대사 효소 유전자형 빈도의 남녀 차이

김성곤,김철민,이덕기,황인복,이현숙,김성연,전은숙,송영상,박제민,최병무,김명정 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : There are a number of preceding epidemiological studies reporting gender differences in the genetic etiology of alcohol dependence. The author investigated gender difference in the frequencies of ADH2 and ALDH2 genoypes between the patients with alcohol dependence and normal control. Methods : The subjects were 141 alcohol dependent patients (104 males, 37 females) and 138 normal control (79 males, 59 females). The frequencies of 1/1 and 1/2+2/2 (2+ afterward) genotypes for ADH2 and ALDH2 were investigated in male and female between alcohol dependence and normal control group. DNA was extracted from WBC in peripheral venous blood and PCR-RFLP method was used out for genotyping. Results : First, the frequency of ADH2 1/1 genotype was significantly higher in alcohol dependent patients than normal control in both genders. Second, while there was no gender difference in the frequency of ADH2 1/1 genotype in normal controls, in the patient group however, the frequency was significantly higher in females than males. Third, in male subjects with alcohol dependence, the frequency of ALDH2 1/1 genotype was significantly higher than in male normal control subjects. On the other hand, in female subjects with alcohol dependence, the frequency of ALDH2 2+ genotype was significantly higher than in female normal control subjects. Conclusion : These results suggest that while the risk of alcohol dependence is predominantly affected by ALDH2 1/1 geno-type in male, the female ADH2 1/1 genotype is mainly associated with the risk of alcohol dependence. This means that there are gender differences in the genetic etiology of alcohol dependence.