Bacillus sp. LJ-25가 생성하는 extracellular chitinase의 정제 및 특성

주동식,조덕제,이정석,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

The extracellular chitinase from Bacillus sp. LJ-25 was pruified Sephadex G-100 gel filtration, DEAE-Cellulose and CM-Spephadex C25 column chromatography. The pruified chitinase showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be about 50KDa. The Optimum pH and temperature for the activity of the purified extracellular chitinase were 7.5 and 35℃, respectively. The activity of chitinase was strongly inhibited by metal ions such as Zn²+, Ba²+, Co²+, Mn²+ and Cu²+. The purified chitinase did not hydrolyze N,N??-diacetylchitobiose, N,N, N'-triacetylchitotriose and p-nitrophenol-N-acetyl-β-D-glucosaminide, which are known to be the substrates of exo-type chitinase. The products in hydolysis of colloidal chitin with the chitinase were N-acetylglucosamine, N,N'-diacetylchitobiose and N,N,N'-triacetylchitotriose.

干拓地 浚渫土의 堆積特性에 關한 硏究

都德鉉,安成鎬,金善柱 건국대학교 산업기술연구원 1995 건국기술연구논문지 Vol.20 No.-

간척지 준설토의 퇴적 특성을 구명하기 위하여 충남 당진군 일원의 서남해안 干拓綜合開發事業 石門地區에서 준설공사를 하고 있는 排水閘門 引水路 浚渫土를 시료로 채취하여 시험분석한 결과를 요약하면 다음과 같다. 1) 本 試驗圃의 토질은 실트질 점토로서 浚渫 排砂管 吐出口 부위별 含泥率은 상부 7.36%, 중간부 18.85%, 하부 50.95%이고 평균값은 25.72%이다. 2) 堆積狀況은 浚渫 排砂管 吐出口를 중심으로 주위 약 30m까지는 粒子가 큰 모래와 점토 덩어리가 퇴적되고, 유속이 작은 원거리에서는 細砂 및 실트가, 精水狀態에서는 粘質土가 퇴적되므로 약간의 유실이 발생되어도 준설시의 유속을 크게 하면 粗粒子만 퇴적되어 양질의 지반을 얻을 수 있는 것으로 나타났다. 3) 浚渫土의 콘支持力 변화는 준설 3개월 후 준설 배사관을 중심으로 40∼60m 범위의 콘支持力(qc)은 2.0kgf/㎠, 一軸壓縮强度(qu)는 0.16~0.20kgf/㎠ 정도로서 超濕地에서 濕地로 支持力 증가 속도가 상당히 빠르고 60m 이상에서의 콘支持力(qc)은 0.5~1.0Kgf/㎠ 정도, 一軸壓縮强度(qu)는 0.02~0.06kgf/㎠로서 매우 연약한 超濕地임을 알 수 있었다. 4) 준설토의 강도증진은 자중압밀에 의한 강도증가보다 건조수축에 의한 강도 증진이 빠르고 크기 때문에 이 특성을 이용해서 浚渫盛土高를 20∼30㎝로 하여 건조수축시킨 후 반복적으로 浚渫盛土를 실시한다면 양호한 지반을 얻을 수 있을 것으로 생각된다. 5) 浚渫 排砂管 설치는 本管에 分岐될 지점에 직경이 다른 티(T형, Y형)를 가설해 두어 排砂管 移設에 필요한 경비를 절감할 수 있고, 排砂管 간격을 30m 전후로 배치하면 地盤改良에 필요한 공사비를 절감할 수 있는 것으로 나타났다. In order to study the deposition characteristics of dredged materials in the reclaimed area, deposition process of dredged materials and soil strength parameters in the Sukmoon Project area, which is located in the southeastern coast, have been analyzed with in-situ tests and laboratory research. The results obtained are as follows: Soil texture of the tested plot was silty clay, and the soil content of discharge flow by the part of pipe outlet was 7.36% at the upper part, 18.85% at the middle part and 50.95% at the lower part. Three different characteristics have been investigator from the observations on the relationship between the discharge flow velocity and soil particle size. Coarse sand and clusters of clay were deposited within the range of approximately 30meters from the outlet of discharge pipe where the flow velocity was relatively high. From 30meters to 60meters, finer particles were accumulated, and beyond this range, very fine clay particles were sedimented. So the results showed that the strength properties of dredged materials could be increased as the discharge velocity increased. In three months after the completion of dredging that was carried out within a range of 40∼60meters from the outlet and at a depth of 1.0m below the ground surface, the cone resistance(qc) and unconfined compression strength(qu) was 2.0kgf/㎠ and 0.16~0.20kgf/㎠, respectively, indicating that the increasing rate of bearing capacity is high. Beyond this range, qc and qu varied 0.5~1.0Kgf/㎠ and 0.02~0.06kgf/㎠, respectively, which is quite soft. Shear strength of the dredged materials increased evidently resulted from the shrinkage of drying rather than consolidation caused by the self-weight of deposition. Setting of the desilting scour pipe needs a "T" or "Y" type branch pipe of a different diameter to save the cost of setting. From the result of this study, it was found that if the desilting scour pipe is built with 30m spacing, the cost for land improvement can be saved.

개에 있어서 耳根部의 Lidocaine 水針에 의한 全身 無痛覺의 誘發

권현주,유건주,이상은,이정연,송근호,김명철,정성목,박성준,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Twelve clinically healthy mongrel dogs were used to clarify the possibility of general analgesia induction by lidocaine aquapuncture to auricular root area in the present study. The experimental animals were devided into control (6 heads) and experimental groups (6 heads), respectively. Physiological salines (3 ml/kg) were injected into both auricular root areas in control group. Lidocaine hydrochlorides (3 ml/kg) were injected into both auricular root areas in experimental group. Superficial pain, the changes of vital signs and blood leukocytes, and PCV were examined on pre, 5 min., 15 min., 30 min, and 60 min. after injection in control and experimental groups, respectively. Analgesia was not detected at all in control group, however, all experimental animals were recumbent and analgesia was generally detected in the auricles, noses, dorsal parts, ventral areas, limbs and tails from 5 to 30 minutes after injection with lidocaine hydrochloride into auricular root areas in experimental group. As for the change of vital signs, body temperature showed decreasing tendency from 5 minutes after lidocaine injection in experimental group. Heart rates and respiration rates were increased on 5 minutes after lidocaine injection and then they showed decreasing tendency in experimental group. No significant difference were found among groups. As for the changes of hematological findings, the numbers of blood leukocytes were increased till 15 minutes after lidocaine injection and then they showed decreasing tendency. PCV values were increased on 5 minutes after lidocaine injection, and then they showed decreasing tendency. However, no significant differences were observed among groups. Considering the above findings, it was thought that general analgesia was possible by aquapuncture with lidocaine hydrochloride into auricular root areas and lidocaine aquapuncture didn’t affect so much to the changes of vital signs and blood.

TCP/IP의 실시간 제어 응용에서의 성능평가

배덕진,김주민,김대원 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

This paper evaluates the performance of TCP/IP in case of real-time control. TCP/IP is well-known as a protocol that can be commonly used in non real-time system because of non-predictability of network latency. To analyse wheter real-time communication is possible or not, a simple system for controlling the motor by using the PID controller is introduced. The computational model of the latency according to each layer is defined. The worst case response time is calculated by the defined model. The minimum control period of the proposed system is determined by its result. Through simulations, we analyse the performance of TCP/IP in case of the real-time control application.

국민 여가활동기회의 효율적인 분배와 생산유인 효과 확대를 위한 스포츠시설 및 스포츠 관련 산업의 관광자원화 방안을 위한 연구

남덕현,김천중,이주형 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The purpose of this study is to examine the recognition and satisfaction of sport tourism as an area of tourism industry, and show the actual status of sport tourism, suggest the strategies to develop sport tourism goods according to sports tourism resources. This study is based on the survey of residents, physical education teachers, tour industry managers and local government employees, who were asked about what impact sports tourism had on local societies and environments. The data obtained from the returned questionnaire were coded and transcribed for a statistical analysis. collected data were analyzed though SPSS 10.0using such statical methods as a descriptive statistical analysis, chi square test, ANOVA test. With the twenty first century upcoming, sport tourism is recognized as a necessity of life for every person in the world. In addition, the rapid changes of social phenamenons such as the changing of a basic sense of value and life style. the improvement of income, the development of traffic systems, communication facilties, and medias, have a great influence on the desire for sport tourism. Compared with the past tourism that were mostly passive and superficial sight seeing tour, sport tourism is an active and exhilarating tour, therefore sport tourism could provide tourist with satisfaction of needs. Sports tourism is sure to become one of major industries. This study was done with the limited data, so the result does not fully represent the comprehensive impact of sport tourism and strategies of sport tourism resources.

光州市 家計의 課外敎育과 經濟的 負擔感에 대한 實態調査

황덕순,주문자 全南大學校家政科學硏究所 1994 生活科學硏究 Vol.4 No.-

This study aims to investigate the actual extracurricular education and economic strains of households. For these purposes, 372 households in Kwangju with students but collegians were surveyed through the questionnaire method. The frequency, percentile, One-way ANOVA in GLM and Chi-square test were used for the data analysis. The Results were as follows: 1. Most of households takes extracurricular lessons(334) and be willing to do (268). Many have concerns about it and need it. 2. The average lesson number is 2 subject and average cost per household is 177,000 won. 3. The average economic strains due to the extracurricular cost is 22 points. Economic strains differ according to the socio-economic variables (e. g. father's educational level, mother's educational level, average income level per month, and to the attitude variables, extracurricular will in future, parent concerns, and needs. Here we can conclude, many households have economic strains due to extracurricular fees. And the economic strains by extracurricular lessons are so undesirable to family growth and development that government have to reform the educational structure. So the social need to the extracurricular lesson will be taken away. But in reality, still extracurricular lessons are so needed that the households must have any strategies to face the economic strains.

SrTiO₃첨가에 따른 (x)BaTiO₃-(1-x)SrTiO₃의 유전적, 전기적 특성

정해덕,장동환,김덕근,홍경진,최창주 木浦大學校 工業技術硏究所 1998 工業技術硏究誌 Vol.8 No.-

In this paper, a ferroelectric material BaTiO3 was added to SrTiO3, then (x)BaTiO3-(1-x)SrTiO3(0.7≤x≤1) ceramics were fabricated with stable electrical properties in high voltage. Microstructures and electrical properties were investigated according to mixing conditions of addition material SrTiO3. As a result of the investigation of all experiments, the shrinkage was excellent in 0.9BaTioO3-0.1SrTiO3. The grain size was increased, the curie temperature was shifted low temperature. The 0.9BaTiO3-0.1SrTiO3 was appeared stable property in temperature vs. permittivity. Also, 0.9BaTiO3-0.1SrTiO3 was showed stable dielectric properties at 25~85[C]. V-I properties of specimen were observed in the temperature range of 21~143[C], were divided into three regions. the region I below 10[kV/cm] was shown Ohmic conduction, the region II from 10 to 30[kV/cm] was explained by the poole-Frenkel emission theory and the region III above 30[kV/cm] was analysed by the tunneling effect.

원자로압력용기장 용접열영향부의 미세조직 변화가 Magnetic Barkhausen Noise 거동에 미치는 영향

김주학,윤의박,문종걸,박덕근,홍준화 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.4

최근 자기적 특성의 변화가 재료의 미세조직적 상태에 민감하다는 보고가 있다. 재료의 미세조직은 상(phase), 결정립의 크기, 석출물 등의 다양한 인자로 구성되며, 본 연구에서는 이러한 인자들을 이용하여 재료의 특성과 Barkhausen noise(BN)의 관계를 연관시키기 위한 시도를 하였다. ASTM A 508 Gr.3 원자로압력용기강을 대상으로 용접열영향부의 미세조직적인 변화를 일곱가지 대표적인 영역으로 구분하였고, 각 영역에서의 미세조직과 기계적특성의 변화를 파악한 후, 각 조건별로 BN을 측정하였다. 통상의 보자력이나 잔류자화 같은 자기적 성질은 큰 변화가 없었지만, BN은 미세조직의 상태에 따라 현격한 변화를 보였다. 결정립과 석출물의 크기가 증가함에 따라 BN은 증가하였고, tempered martensite 보다는 tempered bainite 조직에서 높은 BN을 나타냈다. Recent study has demonstrated that some magnetic properties are sensitive to the microstructural state of material. The ASTM A 508 Gr. 3 reactor pressure vessel steel has various microstructural changes including martensitic and bainitic phases, and various sizes of grain and precipitates in the weld heat-affected zone (HAZ). To correlate the microstructural state with Barkhausen noise (BN), specimens were prepared through simulating various weld thermal cycles using a thermal simulator. The conventional magnetic properties, i.e. coercive force, remanence and maximum induction, did not change significantly. whereas the BN amplitude and energy during a magnetization cycle changed markedly with microstructural state. The BN increased with increasing grain and carbide sizes, and the tempered bainite structure showed higher BN parameter than tempered martensite.

나환자의 불안과우울에 관한 연구

정성덕,민성길,유계준,김주덕,유준 大韓癩學會 1982 癩學 會誌 Vol.15 No.1

비 세균성 설사 환아에서 저나트륨 농도의 경구 전해질 용액(Pedialyte^(�)RS)과 lactose free diet(Isomil^(�))의 치료 효과

이종덕,유정애,이주원,김순겸 최신의학사 1985 最新醫學 Vol.28 No.11