자기장 저속 냉동보관법을 이용한 쥐 치아 치주인대세포의 활성도 검사

안현정,김의성,김진,김덕원,김기열,이찬영,이승종 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

본 연구의 목적은 흰쥐 상악 대구치를 발거하여 자기장 저속 냉동보관법을 이용하여 냉동 시 치주인대세포의 활성도 및 세포 사멸도를 MTT검색법과 TUNEL검사를 이용하여 측정하고자 하였다. 4주령의 암컷 Sprague-Dawley계 된 쥐의 상악 좌우 제1,2대 구치를 발거하여 각군 당 12개의 쥐 치아를MTT검색에 이용하였고 6개의 치아를 TUNEL 검사에 이용하였다. 실험군은 5개군으로 대조군은 즉시 발치군이며 4℃냉장고에서 1주일간 보관한 냉장군, 발치 후 동해방지제 처리과정을 거쳐 -196℃의 액화질소에 넣어 급속 냉동한 액화질소군, 21.7 ㎃, 60 ㎐, 1 G의 자기장을 이용하여 -0.3℃/min 의 속도로 -20℃까지 냉동 후 -196℃로 급속 냉동한 자기장군, -0.3℃/min의 속도로 -20℃까지 냉동 후 -196℃에 급속 냉동한 저속 냉동군으로 나누었다. 보존액은 F medium을 사용했으며 동해방지제로 10% dimethyl sulfoxide (DMSO)를 사용하였다. 치근면을 단위면적으로 표준화하기 위해 MTT측정값을 Eosin 염색 후 530 ㎜에서 측정한 흡광도 값으로 나누었다. TUNEL 검사 시 각 조직슬라이드에서 400배 크기의 현미경 시야에서 임의로 세 부분을 지정하여 정상 세포수와 양성 세포수를 세어 그 비율을 계산하여 각 실험군 당 평균치를 구하였다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후검정으로 Scheffe와 Tukey HSD방법을 썼으며 결과는 다음과 같다. MTT검색에 의한 흡광도를 Eosin염색 후 측정한 흡광도로 나눈 값에서는 자기장군은 즉시 발치군보다 낮은 세포활성을 보였고 (p < 0.05) 액화질소군, 저속 냉동군과는 통계적으로 유의성 있는 차이를 보이지 않았다. 그러나 자기장군은 액화질소군, 저속 냉동군과 함께 냉장군보다는 높은 세포 활성도를 보였다 (p<0.05).TUNEL검사 결과도 자기장군은 즉시 발치군보다 치주인대의 세포사멸도가 높았으나 (p < 0.05) 저속 냉동군과 액화 질소군과는 통계적으로 유의한 차이를 보이지 않았다. 자기장군은 냉장군보다 세포사멸도가 낮았으며 냉장군은 모든 군 중에서 세포 사멸도가 가장 높았다 (p<0.05). The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were groupl (immediately extraction), group 2 (cold preservation at 4℃ for 1 week), group 3 (rapid Cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95 % level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3,4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

小兒 알레르기性 紫斑症

金洪培,孫璨洛,姜德植,具滋薰 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

저자는 1977년 1월부터 1982년 8월까지 5년 8개월간 경북의대 부속병원 소아과에 알레르기성 자반증으로 입원하였던 환아 36예를 대상으로 하여 다음과 같은 성적을 얻었다. 성별 분포는 남녀 각각 50%로서 차이가 없었고 계절별 분포는 상기도감염이 빈번한 봄, 겨울이 72.2%를 차지하였으며, 선행질환으로서는 상기도 감염이 69.4%에서 인지되었고 알레르기성 병력을 가진 환아는 13.9%였다. 임상증상은 피부병변이 100%, 위장관계 증상이 88.9%, 관절증상이 61.1%에서 나타났었다. 신장침범은 33.3%였으며 현미경적 혈뇨가 1예, 현미경적 혈뇨 및 경도의 단백뇨가 3예, 현미경적 혈뇨 및 심한 단백뇨가 2예였다. 육안적 혈뇨 및 경도의 단백뇨는 1예, 육안적 혈뇨 및 심한 단백뇨는 4예가 있었으며, RPGN이 1예였다. 이중 6예에서 신생검을 시행하였으며, 2예는 경도의 병변을 보였고 나머지 4예는 모두 반월체를 형성하는등 심한 병변을 보였다. RPGN 경과를 취한 1예는 사망하였다. 신침범과 이와 관련된다고 생각되는 인자들, 즉 5세 이상의 연령군, 성별, 위장관증상의 경중, 관절증상의 유무, 재발유무 등과의 관계를 살펴본 결과 신침범을 일으킨 환아에서 심한 위장관증상 및 재발율이 다소 높게 나타났으나, 통계적 처리로서는 특별한 의의가 없는 것으로 나타났다. A clinical observation has been made on 36 children with Henoch-Scho¨nlein perpura who were admitted to pediatric department of KNU hospital from January 1977 to August 1982. Rate of male to female patients was 1:1, and the majority of cases developed illness during spring and winter months. URI was noticed as preceding illness in 69.4% of cases, and allergic history was elicited in 13.9%. Clinical manifestations showed involvement of skin in 100%. GIT in 88.9%, joint in 61.9% and kidney in 33.3%. Out of 12 cases in whom nephritis developed, 1 case showed microscopic hematuria, 3 cases microscopic hematurai with mild proteinuria, 2 cases microspic hematuria with heavy proteinuria, 1 case macroscopic hematuria with mild proteinuria, 4 cases macroscopic hematuria with heavy proteinuria and 1 case RPGN. Percutaneous renal biopsy performed on 6 cases revealed minimal change in 2 cases and severe renal involvement with crescent formation in 4 cases. Relationship between renal involvement and other parameters including age grou over 5 years, sex, severity of GIT symptoms and presence of recurrence showed somewhat high incidence of severe GIT involvement and presence of recurrence in patients with renal involement. However statistical analysis showed no significant difference.

제주도 한우의 번식장해 발생원인과 대책에 관한 연구(제3보) : 난소질환 제주한우에 있어서 hormone 처리가 수태에 미치는 영향

김중계,김승찬,장덕지 제주대학교 농과대학 제주도축산문제연구소 1986 畜産論叢 Vol.1 No.1

Acccrding to the investigation of reproductive status and disorders in Cheju native cattle, the delayrd puberty, fist calving age in heifer, and thealternate year calving cow dric to anrstrus following calving were observed more frequently on this island. This study, therefore. was carried out to investigate the effect of PMS as a method of trenstment for the cows with ovarian hypoplasia and atrophy. For the 15 cows with ovarian hypoplasia, the blood was tested and 40 cows of anestrus due to ovarian hypoplasia and atrophy were selected to treat with PMS. The results obtained were as follows. 1. The blood test indicated that cows of the ovarian hypoplasia appeared to havc lower scriim protein and calcium level than that of normal cow. Espercially abnormality inA/G ratio and Ca/P ratio in Holstein was found. 2. Trensment of PMS into 40 cows with the ovaridn atrophy and hvpoplasia showcd estrus average 6.7 days after the final hormone injection. 3. Total 38 cow were conceived by PMS (100 IU) hormone treatment; 26 cows (64.4%) at the first estrus. It cow (30.6%) at the second estrus and the remainder at the third estrus. 4. The above resolts inkicated that treatment of PMS hormone into cow with ovarion hypoplesia and atrophy was effertive for inducing estrus and conception.

개 원발성 면역매개성 혈소판 감소증 증례

유찬,임수정,이선희,박형진,김태신,송은식,김덕환,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 13-year-old, castrated male, Maltese-dog was admitted to the Veterinary Medical Teaching Hospital of Chungnam National University with the history fo petechiae. On physical examination, she had petechiae and ecchymoses at her back, bilateral inguinal region, and inner part of ear pinna. CBC indicated neutrophilia and severe thrombocytopenia. A few platelets, no autoagglutination of RBC, and no spherocyte were observed. Coagulation profile demonstrated normal PT, and aPTT. No significant findings was not found on serum biochemical profile and electolytes test. FNA of a splenic mass which was found on the abdominal radiograph and sonograph demonstrated extramedullary hematopoiesis, Proteinuria was observed on urinarysis. Primary immune-mediated thrombocytopenia was diagnosed by ruling out of other underlying disease, severe thrombocytopenia, and response to immunosuppressive therapy. Prednisone and antibiotics were administered orally and parenterally, respectively. After altering prednisone to azathioprine, the number of platelets was recovered to normal range and petechiae and ecchymoses were improved.

한국과 일본의 정신지체 영역 전공과 운영 고찰

김덕규,박희찬 한국특수아동학회 2003 특수아동교육연구 Vol.5 No.1

본 연구는 한국과 일본의 정신지체 영역 전공과 운영실태를 분석ㆍ비교하는데 목적을 두었다. 연구대상은 한국의 정신지체학교 전공과 21개와 일본의 양호학교 전공과 7개였으며, 조사를 위하여 전공과 관련 학생과 교육을 중심으로 설문지를 개발하였다. 조사된 자료는 문항에 따라 빈도와 비율, 유사점과 차이점으로 분석하였다. 연구 결과는 다음과 같다. 한국과 일본의 정신지체 영역 전공과 간에는 입학자격, 전형방법, 전공과 졸업 후 취업률에서는 유사하였으나, 학급당 학생수와 고등부 졸업 후 전공과 진학률은 한국이 높았다. 또한 교육목표, 교육과정, 수업연한, 주당 수업시수는 양국이 각각 달랐다. 한국에서의 교육목표와 교육과정은 직업인 양성에 중점을 두고 있으나, 일본은 전인격적 발달에 중점을 두고 있었다. 고등부 교육과정과의 연계성에서 한국의 전공과는 고등부와 별도로 운영하는 학교가 많았고, 일본은 고등부와 연계하여 운영하는 학교가 더 많아 양국간 차이가 있었다. 연구결과를 바탕으로 우리나라 정신지체학교 전공과 운영 방향을 제언하였다. This study analyzed and compared postsecondary special school programs for students with mental retardation in Korea and Japan. Investigations were conducted in 21 and 7 post-secondary programs in Korea and Japan, respectively. Questionnaires were developed to survey regarding student and education of the postsecondary programs. Data were analyzed using frequency and ratio in some questionnaire items and using contents analysis of similarities and differences in others. Results of the study showed that little differences were existed between Korea and Japan postsecondary special school programs in terms of entry qualification, entry examination method, and employment rates after postsecondary programs. However, students per class and rate toward postsecondary program from high school were higher in Korea than in Japan. The two countries had different education goals, curriculum, study period, and number of classes per week. Whereas the education goals and curriculum in Korea focused on the development of vocational training, education goals in Japan stressed overall personal development. Based on study results, future directions for postsecondary special school programs to take in Korea were proposed.

MANET 라우팅 프로토콜 QoS 지원서비스의 성능평가에 관한 연구

鄭燦赫,金鉉郁,李光培,姜景仁,朴京培,劉忠烈,吳世德,裵振勝,趙榮泰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we evaluated two main QoS supporting routing protocol on wireless ad hoc network. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS support service between source and destination becoming a very important issue. Of all routing protocols, DSR and AODV are very important routing protocol in MANET. So we simulated DSR and AODV QoS Routing Protocol Through simulation evaluation tool NS(Network Simulation) based on various environments.

META분석을 이용한 유산소운동의 생리적변인에 관한 연구

남덕현,김규수,박찬웅 龍仁大學校 1998 용인대학교 논문집 Vol.16 No.-

The purpose of this study was to analyze the effects of regular aerobic exercise on physiological variable. The results of 145 training studies, conducted of the last 30 years(1966-1996), involving the measurement of % body fat, RHR, VO2max, VE were collected and statistically analyzed using META-analysis method. The major results were as follows, ① physiological effect As aerobic excercise had a significant effect on physiological variables(% body fat, RHR, VO2max, VE), it directed to positive effects. And the specific character variables effecting on physiological variable by aerobic exercise were initial level, exercise duration, weight, age, exercise time and exercise intensity. ② The characteristic variables effecting on physiological variables had on different influence by the sex and subject state.

이동 애드 혹 네트워크에서 QoS를 지원하는 AODV 라우팅 프로토콜

吳世德,趙榮泰,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this paper, we propose a bidirectional mobile Ad Hoc routing protocol based on AODV(ad-hoc on-demand distance victor routing) with QoS(quality of service) support. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS guarantee between end point is a very important issue. The existing AODV routing protocol is implemented to use only BE (Best Effort) service. However, actual wireless mobile environment requires to reduce a waste of communication resources and meet with the real-time change of data paths by setting up routes with QoS support, considering available communication resources at each node in advance. Therefore, in this thesis, we propose an adaptive QoS support method using hello message in order to rapidly meet with the change of available communication resources and to smoothly perform route maintenance. For performance evaluation, we analyzed the average date reception rate. At that time, we used evaluation parameters such as node's mobility -stop time, hello message period, and packet priority, considering the node's mobility and the number of best effort/QoS data flows. As results, we found that with our proposed method the average data reception rate was increased 16.3% for 0 mobility-stop second case and 4.7% for 300 mobility-stop second case.

재생굵은골재를 사용한 콘크리트의 동결융해 특성

성찬용,윤준노,김영익,임상혁,정덕현 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.2

이 연구는 플라이 애시를 결합재 중량의 20%, 재생굵은골재를 천연쇄석굵은 골재의 0%, 25%, 50%, 75%, 100%를 치환하여 압축강도와 동결융해 특성을 구명하였으며, 이 연구를 통해 얻어진 결과를 요약하면 다음과 같다. 1. 재령 28일의 압축강도는 모든 배합비와 W/B 35%에서 400㎏f/㎠이상으로 나타나 고강도콘크리트에 이용될 수 있을 것으로 생각된다. 2. 중량감소율은 동결융해 300사이클에서 1%미만으로 나타났고, 표면박리와 같은 현상은 나타나지 않았으며, 재생골재의 대체율이 증가할수록 감소율은 증가하였다. 3. 초음파진동속도는 동결융해 300사이클에서 19~24%정도의 감소율을 나타내었으며, 재생골재의 대체율이 증가할수록 감소율은 증가하는 경향을 나타내었다. 4. 상대동탄성계슈는 재생골재의 대체율에 따라 60~72%의 범위로 나타내었으며, 재생골재 대체율이 증가할수록 감소하는 경향을 나타내었다. 5. 내구성지수는 모든 배합에서 60이상을 나타내었으나, 재생골재 대체율이 100%일때는 급격히 떨어지는 경향을 나타내었다. 6. 재생굵은골재를 사용한 콘크리트의 압축강도, 중량감소율, 초음파진동속도, 상대동탄성계수, 내구성지수 시험결과를 볼 때, 시험에 사용한 재생굵은 골재는 고강도콘크리트에 사용이 가능할 것으로 판단된다. This study was performed to examine the freezing and thawing properties of the high strength concrete using recycled coarse aggregate. The recycled coarse aggregate was replaced by 0%, 25%, 50%, 75% and 100% of natural crushed aggregate. The compressive strength of the concrete used recycled coarse aggregate was shown in more than 400kgf/㎤ at the curing age 28 days. The weight loss ratio by freezing and thawing was shown in less than 1% at all mix type. The pulse velocity and relative dynamic modulus were decreased with increasing the freezing and thawing cycles. Also, durability factor for the freezing and thawing were decreased with increasing the content of recycled coarse aggregate. But, recycled concrete replaced with recycled coarse aggregate 100% was shown in more than 60 by durability factor in freezing and thawing of 300cycles Accordingly, these recycled coarse aggregate can be used for high strength concrete.

GMAHN 환경에 적응하는 에러 복구기능을 이용한 AODV 라우팅 프로토콜

정찬혁,오세덕,배진승,이기원,장양근,이광배,김현욱,유충렬,하재승 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

Mobile nodes in ad hoc mobile networks typically communicate over wireless channels and are capable of movement. These are networks that support multihop communication and can be formed on a temporary basis. This paper proposes a solution that allows mobile nodes to access the wired Internet and to roam from base station to base station. And we also develop the efficient method that adapts in Global MANET which can be changed over time. It is observed that we can reduce not only error detection time but also network load, thus increasing the data reception rate in Global MANET Environment.