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      • AHCISCOPUSKCI등재
      • 금은화 추출물의 세포독성에 관한 연구

        한종현,백승화,김일광,한두석 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

        In order to investigate the cytotoxicity of Lonicerae Flos extract, their extracts were dissolved in distilled water and methanol. All material diluted from 10^-3to 10^-7(㎎/㎖) with distilled water putted in each well of 24 multidish cultured rat fibroblast for 48hours. The cell number was calculated at 2 days and cell shape takes photographed by inverted microscope at same day. The results were as follows: 1. The rate of cell multiplication was the lowest in water extract from 10^-3 to 10^-7(㎎/㎖) concentration and the highest in ether and hexane extract at 10^-7(㎎/㎖) concentration. 2. Index of cytotoxicity was the highest with 3 score in water extract at 10^-3(㎎/㎖) concentration and the lowest with 1 score in ether, ethyl acetate or hexane extract at same concentration. 3. The degeneration of cell shape and number was the severest in water extract of 10&-4(㎎/㎖) concentration, but ether and hexane extracts in same concentration were weaker than water extract. These results indicate that the cytotoxicity of extracts by ether and hexane from same Lonicerae Flos was weaker then water extract.

      • 웨이트 트레이닝 모델에 의한 운동부하가 흰쥐 장단지근의 핵소체 형성부위 활성과 근섬유의 형태에 미치는 효과

        한두석,천상우,최영자 원광대학교 생체재료·매식연구소 1995 원광생체재료·매식 Vol.4 No.1

        This study was to investigate the nucleolar organizer region(NOR) associated protein by means of silver chloride staining and muscle fiber types in sections stained for SDH in the gastronecmius muscle loaded with movement by klitgaard's weight training model. Ag-NOR technique is a direct measure of the ongoing transcriptional activity of the rRNA gene or their activity. The number of Ag-NORs sites within nucleolus may reflect protein synthetic activity and proliferation. Thirty Spraque-Dawley adult female rats(190-200g) were divided into control and 3 experimental groups, which were trained for 1, 2 and 3 months. Body weight and muscle weight measured at 1, 2 and 3 months. The removed muscle was fixed by immersion, and processed for light microscopy. The results were as follows; 1. Body weight was increased, but muscle weight was decreased with the lapse of time of training. 2. After movement, the mean number of nucleolar organizer regions per nucleus was significantly increased at 2 and 3 months in gastronecmius muscle. 3. The percent of nucleolus include 4 Ag-NOR or more was increased with the lapse of time of training. 4. In muscle fiber types, type A was decreased but type C was significantly increased with the lapse of time of training.

      • 授乳期間中 家兎 乳腺組織內 大식細胞의 動態에 關한 形態學的 觀察

        韓斗錫 圓光大學校 1986 論文集 Vol.20 No.2

        本 硏究는 E. coli를 注入한 家兎의 乳腺組織內에서 大? 細胞의 動態를 觀察하기 위하여 實施하였다. 乳腺組織의 病理學的 所見은 E. coli를 注入한 후 經時的으로 觀察하였으며, 大 細胞의 動態는 E. coli를 注入하여 炎症이 惹起된 乳腺內에 Trypan blue와 indian ink를 注入한 후 觀察하였다. 그 결과는 다음과 같았다. E. coli의 浮游液을 注入한 群에서는 共히 肉眼的으로 乳腺의 腫?, 充血 및 出血, 또한 限局性 壞死?와 結이 있었다. 組織學的으로는 初期에 腺胞와 乳管內에 多數의 好中球의 出現과 上皮細胞의 變成, 壞死 및 脫落], 間質內에 好中球의 浸潤, 出血 및 水腫이 觀察되었다. 時間이 經過함에 따라 ??芽細胞의 增殖과, 淋巴球, 好?球 및 大 細胞의 出現이 있었으며, 동시에 이들 細胞成分으로 構成된 限局性 壞死?가 觀察되었다. 肉芽腫性 變化가 7일째부터 認定되었다. 健康한 家兎의 授乳末技 乳腺組織의 綜合組織內에서는 授乳初期의 乳腺組織에 比하여 Trypan blue와 indian ink 粒子를 ?食한 大 細胞가 多數 觀察되었다. 그리고 炎症初期의 炎症? 周圍에서는 大 細胞를 少數를 認定할 수 있었으나 炎症이 進行됨에 따라 多數의 大 細胞가 炎症? 周圍에 出現하였다. The present study was undertaken to clarify the migration of macrophage in the mammary gland of rabbits with E. coli strains. The pathological changes were observed in mammary gland experimentally induced with E. coli strains and the migration of macrophage after injection of trypan blue or ink was examined in the mammary glands of healthy lactating rabbits and of the experimentally infected rabbits. From the results reported, the following points are ocncluded. In the bacterial suspension infused by E. coli, the affected quarters of udder showed grossly swelling, hyperemia, hemorrhage, focal necrosis ad firmnes. The microscopic findings of early stage of the mammary gland were appearance of large numbers of heterophils in the glandular lumina and ducts accompanied by degeneration, necrosis and desquamation of epithelial cells, and also infiltration of heterophils, hemorrhage and edema in the interstitial tissue, and destruction of alveoli. later, proliferation of fibroblasts, plasma cell, lymphocytes, eosinophils and macrophages appeared in the glandular tissue, and with these cells necrotic foci of glandular tissue were surrounded by highly proliferated connective tissue. in addition, glanulomatous inflammatory changes could be observed in the glandular tissue from the 7th day after infusion. Macrophages phagocytized the particles of trypan blue or indian ink were increased in number in the glandular tissue of early lactating period as compared with the later lactating period in the normal rabbits. also numerous macrophages appeared around the inflammatory foci in the course of advance of inflammation induced by bacterial suspension.

      • KCI등재후보

        DSP를 이용한 비선형 타이밍벨트 구동시스템의 QLQG/LTR 제어

        한성익,방두열 한국공작기계학회 2001 한국생산제조학회지 Vol.10 No.4

        In this paper, the QLQG/LTR control method is applied for the position control of the nonlinear timing belt diving sys-tem. Parameters of the plant are identified by genetic algorithm and nonlinear elements, such as Coulomb friction and dead-zone, are quasi-linearized by RIDF method. Comparing with the LQG/LTR control, the QLQG/LTR has similar structures of the LQG/LTR, but this method can consider nonlinear effects in designing the controller. Thus, the QLQG/LTR control system is robust to hard nonlinearities such as Coulomb friction. dead-zone, etc. For a given hard non-linear system, through experiments, it is shown that the tracking performance of the QLQV/LTR control system can be very improved than the LQG/LTR control system.

      • KCI등재
      • 참깨 立枯性 病原菌에 관한 硏究

        李斗珩,梁壯錫,韓東旭 서울市立大學校 1986 論文集 Vol.20 No.2

        Damping-off and wilting diseases in sesame plant(Sesamum indicum L.) occurring severely in Korea have been studied for incidences of disease from seeds, diseased plants and soils. Alternaria longissima, A. tenuis, Aspergillus. spp, Cephalosporium sp., Cuvuaria lunata, Drechslera sp.,sp., Fusarium equiseti, F. moniliforme, F. semitectum, F. solani, Mucor sp., Myrothecium roridum Penicillium spp., Phoma sp., Rhizopus sp., were saprophytic fungi and Alternaria sesami, A. sesamicola, Macrophomina phaseolina, Rhizoctonia solani were pathogenic fungi detected from 66 seed samples of sesame. Initial symptoms caused by pathogenic fungi on seedlings from naturally infected seed by the test tube agar method have been described and discussed. Except Alternaria sesamicola, A. tenuis and Macrophomina phaseolina, all fungi were completey wiped out the infection by pretreatment with Benlate-T. The pathogenic fungi isolated from sesame soil were Fusarium oxysporum and Rhizoctonia solani. F.oxysporum occurred less frequently in upland rice and ground nut soils than in sesame soil, but R. solani not decreased by upland rice and ground nut soils. The differences of the isolation frequency of F.oxysporum and the incidence of the wilting plants were found among the fields which varied in their cropping histories. F.oxysporum isolated most frequently from stems and roots of wilting sesame plants followed by R. solani. Nematodes also isolated from the wilting sesame plants.

      • 다원 트래픽을 위한 신경회로망 기반의 ATM호 수락 제어

        이두헌,신요안,김영한 崇實大學校 生産技術硏究所 1995 論文集 Vol.25 No.2

        This paper is concerned with a new approach to adaptive call admission control based on a neural network for multiple service classes with different quality of service (QoS) in the ATM-based Broadband Integrated Service Digital Networks. Our approach aims to deal with different cell loss rates as QoS for multiple service classes having different traffic characteristics. The cell loss rate for each service class is simultaneously controlled by considering the target cell loss rate of each class and the trunk capacity. We extend Hiramatsu's neural network based on-line controller for the single QoS[1] by constructing multiple pattern tables based on each service's acceptance or rejection at the call set-up requests. Computer simulation results show effectiveness of our adaptive call admission controller for service classes with different QoS.

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