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Accurately evaluating cylindricity deviation is the most important requirement for the quality analysis and control of cylindrical products in modern industrial manufacturing processes. The cylindricity deviation of a whole cylinder is usually evaluated with measured points from the target surface. In practice, there are many local intrinsic characteristics that can be related to defects or manufacturing errors, and some precision cylindrical workpieces such as large bearings and rollers are very sensitive to the local cylindricity quality. Hence, it is necessary to evaluate and analyze the local cylindricity variation. In previous works, few methods have focused on the deviation evaluation of local patches of the measured workpieces, and variation of local characteristics could not be directly reflected. In this paper, we propose a new statistical evaluation method for cylindricity deviation using local least squares reference cylinder, which not only evaluates the local and global cylindricity deviations, but also presents deviation change features. This paper illustrates the theoretical basis of the statistical evaluation method, provides the evaluation operation process and parameters, and finally demonstrates the accuracy and feasibility of the proposed method according to the experimental results. The proposed method can be effectively used to provide a reliable verification of the product quality and to analyze the error sources in manufacturing and inspection phases.
Laccases are multicopper oxidases with important industrial value. In the study, a novel laccase gene (mco) in a Staphylococcus haemolyticus isolate is identified and heterologously expressed in Escherichia coli. Mco shares less than 40% of amino acid sequence identities with the other characterized laccases, exhibiting the maximal activity at pH 4.0 and 60oC with 2,2'-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) as a substrate. Additionally, the Mco is tolerant to a wide range of pH, heavy metal ions and many organic solvents, and it has a high decolorization capability toward textile dyes in the absence of redox mediators. The characteristics of the Mco make this laccase potentially useful for industrial applications such as textile finishing. Based on BLASTN results, mco is found to be widely distributed in both the bacterial genome and bacterial plasmids. Its potential role in oxidative defense ability of staphylococci may contribute to the bacterial colonization and survival.
Ultrasound and vacuum were respectively employed to enhance CO2 desorption in a water scrubbing biogas upgrading system. Results showed that incomplete CO2 desorption could cause a high CO2 content in the water and seriously affect the purity of the product gas. Vacuum had a strong enhancement effect on CO2 desorption. When a vacuum of 0.04MPa was used to enhance CO2 desorption, the amount of the stripping air could be reduced to 1/16- th of that without enhancement, indicating that vacuum could greatly enhance CO2 desorption and significantly decrease the amount of the stripping air, which was expected to reduce a large amount of energy consumption. In contrast, the enhancement effect of ultrasound was not so obvious for CO2 desorption in the desorption column with air stripping, since the solution could be well desorbed by gas stripping, though ultrasound could strongly affect the static CO2 desorption.
Laccases are multicopper oxidases with important industrial value. In the study, a novel laccase gene (mco) in a Staphylococcus haemolyticus isolate is identified and heterologously expressed in Escherichia coli. Mco shares less than 40% of amino acid sequence identities with the other characterized laccases, exhibiting the maximal activity at pH 4.0 and 60℃ with 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) as a substrate. Additionally, the Mco is tolerant to a wide range of pH, heavy metal ions and many organic solvents, and it has a high decolorization capability toward textile dyes in the absence of redox mediators. The characteristics of the Mco make this laccase potentially useful for industrial applications such as textile finishing. Based on BLASTN results, mco is found to be widely distributed in both the bacterial genome and bacterial plasmids. Its potential role in oxidative defense ability of staphylococci may contribute to the bacterial colonization and survival.
Drought stress is one of the most important abiotic stresses. Cotton is classified as drought tolerant crop but the regulatory mechanism is unknown. MicroRNAs (miRNAs) have been implicated important roles in stress responses in many plants. However, the study of miRNAs in cotton responsive to drought stress is limited, especially in early-maturing cotton. In this study, we performed deep sequencing of small RNAs to identify known and novel miRNAs involved in the regulation of drought stress and understand the expression profile of miRNAs in early-maturing cotton. Three cotton small RNA libraries: non-stressed Shizao1 (early-maturing cotton variety) library (NSS), drought-stressed Shizao1 library (DSS) and non-stressed Jimian958 (medium-maturing cotton variety) library (NSJ) were constructed for deep sequencing. As a result, we identified a total of 64 known and 67 novel miRNAs in the 3 libraries and 88 of them were dramatically differentially expressed (greater than twofold) during drought stress. In addition, we found the expression of 41 miRNAs increased or reduced more than twofold in early-maturing cotton variety compared with that in medium-maturing cotton variety. Our results significantly increased the number of miRNAs in cotton and revealed for the first time the expression profile of miRNAs for early-maturing cotton.
The microstructural and magnetic properties of melt-spun ribbons with thecomposition (CexNd100-x)30FebalCo4Ga0.2B0.92 (where x = 50, 60, 70, 80, and 90)were investigated. The ribbons were examined with Scanning ElectronMicroscopy (SEM), Transmission Electron Microscopy (TEM), and VibratingSample Magnetometry (VSM). It was found that the grain size of the ribbons ison the nanometer scale, and the grain size decreases with decreasing Ce content. The magnetic hysteresis loops showed that the magnetic properties of theribbons gradually deteriorated with increasing Ce content. This is because themagnetic polarization (Js) and magnetocrystalline anisotropy field (HA) ofCe2Fe14B are smaller than those of Nd2Fe14B. Furthermore, from the initialmagnetization curve it was found that increasing the Ce content changes thecoercive force mechanism to the nucleation mechanism. When Ce contentaccounts for 90% of total rare earth metals, the coercive force mechanismmainly appears to be a nucleation mechanism.
Dong, Liang,Li, Feng,Piao, Yongzhe,Sun, Dong,Zhao, Rui,Li, Cheng,Cong, Lina,Zhao, Changxin The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
High sugar concentration culturing was commonly used in modern fermentation industry. However, it leads to the reduction of the foam stability in beer brewing due to the excess secretion of proteinase A. To better understand the characterization of proteinase A excretion from Saccharomyces cerevisiae in high sugar stress conditions, the cultures were grown by using YNB medium with a high concentration of glucose. Pro-PrA isolated from the medium was purified by gel exclusion chromatography, and the PrA activity was detected using fluorescent substrate analysis. The relative molecular weight of the purified PrA and pro-PrA was estimated at 42 and 54 kDa, respectively, by SDS-PAGE. It indicated that the metabolic behavior of PrA in the high glucose culturing was quite different from the normal conditions, and glucose concentration may have a big influence on its secreted process. Further study showed that PrA was released at the logarithmic growth phase of the culturing, and the amount of PrA was 11 times higher compared with the normal culturing. PrA was considered to be activated by itself under acidic conditions. And it was also confirmed in this work that the step-wise pathway for the autoactivation known as a pseudo-PrA has a major contribution to the autoactivation process of PrA zymogen outside the cell.