음나무(Kalopanax septemlobus Koidz.) 잎 추출물의 폴리페놀 및 항산화효과

전동하,이진태,천순주,이창언,김태훈,이도형,한진규,김세현,Jun, Dong-Ha,Lee, Jin-Tae,Cheon, Soon-Ju,Lee, Chang-Eon,Kim, Tae-Hoon,Lee, Do-Hyung,Han, Jin-Gyu,Kim, Sea-Hyun 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.4

음나무 잎 추출물의 생리활성을 분석하기 위해 열수추출물과 70% EtOH 추출물의 폴리페놀의 함량을 측정하였으며, 항산화활성 분석을 위해 전자공여능, nitric oxide radical 저해활성, xanthine oxidase 저해활성, ABTS radical cation decolorization을 측정하였다. 폴리페놀의 총 함량은 열수추출물과 70% EtOH 추출물에서 각각 104 mg/L, 125 mg/L으로 정량되어 70% EtOH 추출물이 열수추출 물에 비해 높은 함량을 보였다. DPPH radical 소거능은 70% EtOH 추출물이 1,000 ppm에서 93.1%로 나타나 같은 농도의 BHA와 유사한 소거능을 보였다. nitric oxide radical 저해활성은 70% EtOH 추출물과 열수추출물이 1,000 ppm에서 49.6%, 49.5%로 나타나 같은 농도의 BHA보다 높은 nitrit oxide radical 저해활성을 보였다. Xanthine oxidase 저해활성은 1,000 ppm농도에서 열수 추출물과 70% EtOH 추출물에서 각각 46.6%, 60.4% 활성을 나타내었다. ABTS radical cation decolorization 활성은 1,000 ppm에서 열수추출물이 92.7%, 70% EtOH 추출물이 91.4%로 나타났다. 이상의 결과로 보아 음나무 잎의 70% EtOH 추출물과 열수추출물 모두 많은 폴리페놀이 함유되어 있으며, 70% EtOH 추출물과 열수추출물 모두 합성항산화제에 비해 항산화 활성이 다소 낮지만 천연 항 산화제로 우수한 항산화활성을 나타내는 것으로 분석되었다. 이를 바탕으로 음나무 잎의 강한 항산화능을 피부노화 예방관련 미용식품 및 기능성 화장품 소재로 활용 가능한 유용한 수종으로 개발 될 수 있을 것이다. The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

유산균 발효에 의한 밤송이추출물의 항산화 효과

전동하(Dong Ha Jun),조우아(Cho Woo-A),Jae Bong Lee,장민정(Min Jung Jang),유미숙(Mi Suk You),박정렬(Jung Youl Park),김세현(Sea Hyun Kim),이진태(Jin Tae Lee) 한국생명과학회 2014 생명과학회지 Vol.24 No.11

국내의 천연자원 및 부산물로부터 기능성을 갖는 다양한 물질을 탐색하여 자원의 효율적인 측면, 새로운 기능성을 함유한 화장품소재 개발 및 피부 노화 메커니즘을 연구함으로써 밤과수 과정에서 폐기되는 밤송이의 활용방안을 찾기 위한 연구의 기초 자료로서 그 화학성분을 분석하였으며, 또한 새로운 기능성 소재를 탐색하기 위한 일환으로 밤송이 발효 추출물을 이용하여 항산화 효과에 대한 연구를 하였다. DPPH 라디칼소거능, Hydrogen peroxide저해활성, Nitric oxide radical 소거능 측정결과 균주 5% 접종 후 6일간 배양액 추출에서 가장 높은 항산화활성을 나타내었다. 또한 ABTS 라디칼소거능, 환원력 측정, Superoxide anion radical 저해활성 실험을 통해서 대조군으로 사용된 BHA와 Ascrobic acid, EGCG에 비교하여 발효밤송이 추출물은 유사한 항산화 활성을 나타나는 결괄를 얻었다. 이와 같은 결과로 미루어 보아 발효 밤송이 추출물은 효능이 좋은 천연 항산화제로 기능성 화장품의 소재로 개발될 가능성이 매우 높다고 사료된다. The aim of this study was to show the antioxidant properties of chestnut (Castanea crenata) bur extracts fermented by Lactobacillus casei. The chestnut has been used as a cosmetic material in Korea for many years. This study showed that reactive oxygen species (ROS) were inhibited by the fermentation materials of chestnut bur extracts. The antioxidant activities were analyzed and expressed as EDA, ABTS, reducing power, SOD-like activity, hydrogen peroxide scavenging activity, superoxide anion radical scavenging activity, and nitric oxide scavenging ability. The antioxidant activities of fermentation materials from L. casei of chestnut bur extracts were higher than those of butylated hydroxyanisole (BHA), epigallocatechin gallate (EGCG), and ascorbic acid (AA). Therefore, we expect that fermentation materials from L. casei of chestnut bur extracts are valuable resources as natural antioxidants and functional cosmetics ingredients.

율피추출물의 사람 비만세포주 사이토카인 발현 억제 효과

전동하 ( Dong Ha Jun ),장영아 ( Young Ah Jang ),김희영 ( Hui Yeong Kim ),김수진 ( Su Jin Kim ),김진철 ( Jin Chul Kim ),김세현 ( Sea Hyun Kim ),권대준 ( Dae Jun Kwoen ),한상익 ( Sang Ik Han ),이진태 ( Jin Tae Lee ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.2

Objectives: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by eczematous inflammtion of the skin. The chestnut inner shell extracts (CI) has been used as a cosmetic material for a long time in Korea. However, the precise anti-allergy effects of CI have yet to be clearly elucidated. In this study, we attempted to evaluate the effect of CI on mast cell-mediated allergy inflammation. Methods: To find the anti-allergy and inflammatory effect of CI, we investigated the inhibitory effect of CI on the production of inflammatory mediators using by enzyme-linked immunosorbent assay in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (A23187) stimulated-human mast cell (HMC-1). Results : In this study, we found that CI did not show cytotoxic effect at up to 10 ug/ml on HMC-1. CI inhibited the production of tumor necrosis factor (TNF)-a, interleukin (IL)-6 and IL-8 in stimulated HMC-1. Maximal rate of TNF-a, IL-6 and IL-8 inhibition by CI (10 ug/ml) were about 47.6%, 44.1% and 22.5% respectively. In addition, we showed that Fr.3 isolated from n-Butyl alcohol layer of CI attenuated the production of TNF-a, IL-6 and IL-8 in HMC-1. Conclusion: Taken together, the findings of this study provide us with a novel insight action of CI as a potential molecule for use in the treatment of allergic inflammation diseases.

Design of a W-band Groove Polarizer

Dong-Ha Jun(전동하),Myung-Sook Jung(정명숙),Bierng-Chearl Ahn(안병철) 한국정보기술학회 2016 한국정보기술학회논문지 Vol.14 No.4

A groove polarizer in a circular waveguide has the advantage of structural simplicity and moderately wide bandwidth, which is particularly useful at high millimeter-wave frequencies where manufacturing tolerances are critical. This paper presents design, fabrication and measurements of a W-band groove polarizer in a 2.2mm diameter circular waveguide. First, a design procedure of the polarizer is presented for optimum performance. Next, a W-band groove polarizer is designed for low axial ratio and good impedance matching. The designed polarizer has been fabricated using a numerically controlled machining center. Test of the fabricated polarizer has shown fair agreements between the design and the measurement.

버섯복합물의 항산화 효과

전동하(Dong Ha Jun),김희영(Hui Yeoug Kim),한상익(Sang Ik Han),김영훈(Young Hun Kim),김세기(Se Gie Kim),이진태(Jin Tae Lee) 한국생명과학회 2013 생명과학회지 Vol.23 No.3

이 연구에서는 동충하초, 영지, 상황 버섯 복합물의 항산화 효과를 평가하였다. 3가지 버섯을 각각 70% 에탄올 추출하여 80:10:10의 비율로 혼합하여 평가를 실시하였다. DPPH radical을 이용한 전자공여능 측정에서는 버섯 복합물이 500 μg/ml의 농도에서 66%의 효과를 나타내었고, ABTS radical cation decolorization 측정은 50 μg/ml에서 73%, 500 μg/ml에서 대조군인 BHA와 유사한 효과를 나타내었다. Superoxide anion radical 소거능 측정은 버섯 복합물 10 μg/ml에서 41%로 대조군인 BHA에 비해 3배 이상의 높은 효과가 있었고, 50 μg/ml, 100 μg/ml에서도 BHA 보다 효과가 높았으나, 500 μg/ml에서는 낮은 효과를 나타내었다. Lipid oxidation 측정 결과 Cu<SUP>2+</SUP>군보다 Fe<SUP>2+</SUP>군이 지방 산패 억제능이 뛰어났다. SOD 유사활성능 측정은 500 μg/ml에서 13%의 낮은 효과를 나타내었다. 이 결과, 버섯 복합물의 항산화 효과가 아주 우수했으므로, 항산화 소재로서의 가능성을 확인할 수 있었다. In this study, we evaluated the antioxidant effects of Cordyceps, Ganoderma lucidum (Curtis) P. Karst and Phellinus linteus complexes. Three mushrooms were extracted using 70% ethanol, respectively, in ratios of 80:10:10(?). The electron-donating ability was measured using the DPPH radical mushroom complexes and showed effects of 66% in 500 μg/ml. ABTS radical cation decolorization measured 73% in 50 μg/ml and had an effect similar to BHA in 500 μg/ml. In their superoxide anion radical scavenging ability, the mushroom complexes showed an effect of 41% in 10 μg/ml. The effect on the control of BHA was more than three times as high as BHA in 50 μg/ml and 100 μg/ml concentration. The results showed that lipid oxidation is an excellent inhibitor of lipid oxidation Cu<SUP>2+</SUP>, compared to the Fe<SUP>2+</SUP> group. The results of superoxide dismutase (SOD) activity in the mushroom complexes showed a low effect of 13% in 500 μg/ml. Thus, the results of this study showed that the antioxidant effect of the mushroom complexes was excellent, indicating their potential as an antioxidant material.

헛개나무 추출물의 화장품 생리활성에 관한 연구

김세현 ( Sea Hyun Kim ),전동하 ( Dong Ha Jun ),장민정 ( Min Jung Jang ),이진태 ( Jin Tae Lee ),이창언 ( Chang Eon Lee ),한진규 ( Jing Yu Han ),김진철 ( Jin Chul Kim ),이도형 ( Do Hyung Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.6

Hovenia dulcis var. koreana Nakai has been reported to liver function improvement effect as functional materials for food and medicine. On these facts, biological activity and safety test were conducted to evaluate biological activities of the fruit petiole and root extracts of H. dulcis as a potential cosmeceutical ingredient. Cosmeceutica activities of different extracts were examined by l.l-diphenyl-2-picrylhydrazyl (DPPH) radical generation, the ABTS+ cation decolorization, tyrosinase activity, collagenase activity and elastase activity compared with the properties of the commercial antioxidant butylated hydroxytoluene (BHT) and L-ascorbic acid(AA). The antioxidant activities HDFW, HDFE, HDRW and HDRE were 83.6%, 39.6%, 85.9% and 74.5% in DPPH assay, 99.5%, 13.7%, 96.4% and 88.6% in ABTS assay. Tyrosinase inhibitiory activities HDFW were 56.0% at 1,000 ppm. Measured the inhibition effect of the H. dulcis about collagenase and elastase where break the peptide bonds in collagen and enzyme from the class of proteases where exists in the dermis. The H. dulcis was inhibition the two kind enzymesm, collagenase activities being on a high scale inhibition, was same concentration. Uses the anti oxidation effect and a anti-wrinkle effect of this resultant H. dulcis and with the functional cosmetics use is thought with the fact that will be possible.

밤나무잎의 항주름 효과

장민정(Min Jung Jang),전동하(Dong Ha Jun),김세현(Sea Hyun Kim),한상익(Sang Ik Han),이진태(Jin Tae Lee) 한국생명과학회 2013 생명과학회지 Vol.23 No.6

본 연구는 노령목의 산림의 경제적 가치를 증진하고자 밤나무 잎에 함유된 물질의 기능성화장품 원료로의 기능을 규명하여 화장품 산업에 적용하기 위하여 천연소재로써의 이용 가능성을 살펴보았다. 항산화능 측정을 위한 실험으로 DPPH radical 소거능과 ABTS cation radical 소거능을 실시하였으며, 양성 대조군으로는 항산화 작용이 있는 것으로 알려진 BHA를 이용하여 밤잎 추출물의 항산화 효과를 비교하였다. 그 결과 아세톤 추출물의 경우 시료 농도에 따라 DPPH radical 및 ABTS radical 소거 증가 정도가 컸으며, 열수 추출물은 10 ug/ml의 농도에서 50% 이상의 소거활성을 나타내었다. 이러한 결과로 미루어 볼 때 밤잎 추출물은 항산화 작용이 높고 피부에서 흔히 일어나는 활성산소에 대한 노화 방지에 도움이 될 것으로 사료된다. 주름개선 효과 측정으로 elastase 저해활성을 측정한 결과 아세톤과 열수 추출물에서는 그 효과가 없었으나 에탄올 추출물 100 ug/ml에서 64.6%의 우수한 elastase 저해활성을 나타내었으며, collagenase 저해활성을 측정한 결과 3가지 추출물은 100 ug/ml에서 열수 추출물 18.0%, 아세톤 추출물 14.3%, 에탄올 추출물 5.3%의 순으로 collagenase 저해활성능을 나타내었으며, 다른 천연물질을 이용한 연구결과와 비교 시 밤잎 추출물은 주름개선에 우수함을 확인 할 수 있었다. 주름개선 원료로의 이용가치가 있을 것으로 보인다. 이상의 연구 결과로 보아 밤잎은 항산화 및 항노화 효소활성을 갖는 천연소재로서 주름개선 소재로 활용 될 수 있을 것으로 사료되며, 임삼 효능 평가 등의 계속적인 연구를 통해 기능성 화장품 소재로 개발된 가능성이 매우 높다고 사료된다. This research old age chestnut tree that loss function chestnut leaf renewal efficiency enlargement and development of cosmeceutical product by purpose. Chestnut leaf (CL) exhibits numerous pharmacological effect including anti-allergy and anti-microbial properties. However, the anti-wrinkle effect of CL is not completely understood. In this study, to find a possible explanation for the anti-wrinkle effects of CL, we evaluated the effects of CL on radical scavenging activity, elastase inhibition activity and collagenase inhibition activity. CL was extracted with various solvents including water (CLW), 70% ethanol (CLE) and 70% acetone (CLA). The results showed that CLW, CLE, and CLA have the radical scavenging activity. In addition, we showed that CLW, CLE, and CLA have the elastase inhibition activity and collagenase inhibition activity. Consequently, the CL has a potent anti-wrinkle effect and it could be a useful cosmeceutical product for anti-aging purposes.

유전체 봉과 격막 편파기를 이용한 이중 원편파 피드

왕가남(Jianan Wang),홍기표(Ki-Pyo Hong),전동하(Dong-Ha Jun),유상길(Sang-Gil Yu),안병철(Bierng-Chearl Ahn) 한국정보기술학회 2015 한국정보기술학회논문지 Vol.13 No.9

In this paper, a dual-circularly polarized feed is proposed, which operates at 54-64GHz and consists of a square waveguide, a dielectric rod radiator, a septum polarizer and waveguide bends. A stepped taper is applied to the dielectric rod for the required gain and reflection coefficient. Heights and lengths of five steps on a thin metal plate polarizer inserted in the square waveguide are optimized to obtain the desired reflection coefficient at each port, the isolation between ports, and the axial ratio. E-plane bends are connected to the output parts of the septum polarizer for connection with the WR-15 waveguide. Fabrication and measurement show that the proposed feed has a reflection coefficient of less than -10dB and a transmission coefficient of less than -9.5dB at 57-64GHz, and at 60GHz, a maximum gain of 16.8dBic, a 15-dB taper beamwidth of 60°, an axial ratio of less than 2dB within ±30° from the feed axis.

LF 대역 코일 안테나 자계강도 측정장치

이찬희(Chan-Hee Lee),차재호(Jae-Ho Cha),전동하(Dong-Ha Jun),방재훈(Jae-Hoon Bang) 한국정보기술학회 2016 한국정보기술학회논문지 Vol.14 No.4

In this paper, a measuring apparatus for LF-band coil magnetic field strengths is proposed to enhance its productivity. The proposed apparatus consists of a transceiver circuit connected to test and receiving coils, two MCU chips for controlling the transceiver circuits and monitoring measured voltage/current, and an LCD monitor for displaying measured values. The coil calibration constant k is experimentally obtained in order to calculate the magnetic field strengths from the induced voltage on a receiving coil. A current monitoring circuit and a full bridge switching circuit are employed for accurate measurement of the induced voltages on the receiving coil. An experimental method is carried out to verify the performances of the fabricated apparatus. The measured magnetic strength of a test coil shows a error of less than 3.5% between the fabricated apparatus and the experimental method.

포도 전정가지의 화장품 소재로서의 응용

양재황(Jae Hwang Yang),백성환(Baek sung hwan),박동우(Dong Woo Park),전동하(Dong Ha Jun),김극준(Kim Geuk-Jun),장민정(Min Jung Jang) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

포도는 전 세계적으로 가장 많이 재배되는 과수 중의 하나로 포도 및 포도 가공제품에 대한 성분분석, 항산화, 항암 등 다양한 생물학적 효능 연구가 이루어지고 있으나 겨울처 전정되어 버려지는 전정가지에 대한 연구는 미미한 실정이다. 본 연구에서는 포도전정가지 추출물(GPSE)의 총 폴리페놀함량, 항산화활성, 미백 및 주름개선효과를 검증하였다. 먼저 HPLC분석에는 GPSE는 614.05 ug/g의 trans-resveratrol을 함유하고 있는 것으로 관찰되었다. DPPH 라디칼소거능, ABTS 라디칼소거능 등의 항산화 실험을 통해서 대조군으로 사용된 BHA와 L-ascrobic acid에 비교하여 GPSE은 높은 항산화 활성을 나타내었다. 미백활성 측정을 위한 tyrosinase 저해활성 실험에서도 GPSE는 15.35%, arbutin은 1.48%로 GPSE의 미백효과가 높게 나타났으며, 주름개선 실험에서는 대조군과 비슷한 효과를 가지고 있는 것을 확인 하였다. 이와 같은 결과로 미루어 보아 GPSE는 trans-resveratrol 함량이 높은 천연 항산화제로 기능성 화장품 및 식품으로의 가능성을 확인하였다. Grape pruning stems constitute a scarcely investigated class of byproducts with limited reports on their bioactive polyphenol content and/or industrial applications. Herein we present the outcome of our investigation on grape pruning stems extracts, concerning the assessment of their total polyphenolic content and the detailed evaluation of their antioxidant properties. Results obtained indicate that grape pruning stems are particularly rich in flavonoids and trans-resveratrol. The antioxidant activities was analyzed and expressed as electron donating ability, ABTS cation radical decolorization, hydrogen peroxide scavenging activity and superoxide anion radical scavenging activity, the antioxidant activity of Vitis labrusca L. pruning stems extracted (GPSE) was higher than that of BHA and L-ascrobic acid. The whitening and anti-wrinkle activities display an capability. Results herein grape pruning stems used as a valuable resource for the extraction of resveratrol, which would be added to functional cosmetics and food materials.