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임유진,이상명,김동수,김준범,최광식,이신혜,박정규,이동운,Lim, Eu-Gene,Lee, Sang-Myeong,Kim, Dong-Soo,Kim, Joon-Bum,Lee, Shin-Hye,Choi, Kwang-Sik,Park, Chung-Gyoo,Lee, Dong-Woon 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.1
솔껍질깍지벌레는 우리나라 곰솔림의 주요한 해충의 하나이다. 본 연구는 우리나라에서 솔껍질깍지벌레의 분포를 알아보기 위하여 7개도 3개 광역시, 91개 시군구, 686 읍면동의 해송림에서 2010년 밀도를 조사하였다. 5개도 2개 광역시에서 솔껍질깍지벌레가 서식하고 있었는데 시군구별로는 조사대상의 64.8%인 59개 시군구에서 서식이 확인되었다. 솔껍질깍지벌레의 분포는 남해안 전역과 동해안의 포항, 서해안의 충남 보령지역까지 분포하였다. 충북과 대전광역시 및 제주도에서는 솔껍질깍지벌레의 분포가 확인되지 않았다. 부산지역은 16곳의 모든 구에서 솔껍질깍지벌레가 분포하였고, 시군 단위에서는 전남 해남군의 솔껍질깍지벌레 평균밀도가 1.713마리/0.785 $cm^2$로 가장 높았으며 읍면동 단위에서는 해남군 송지면이 6.36마리/0.785 $cm^2$로 가장 높았다. 곰솔 가지 내의 솔껍질깍지벌레 밀도와 솔껍질깍지벌레가 서식하고 있는 빈도와는 높은 상관관계가 있었다(상관계수=0.89). The black pine bast scale, $Masucoccus$ $thunbergianae$ (Hemiptera: Margarodidae), is a serious pest of the Japanese black pine, $Pinus$ $thunbergii$, in Korea. The distribution of the black pine bast scale was examined, looking overall at 686 towns (eup), townships (myeon) or neighborhoods (dong). There were Japanese black pine ($Pinus$ $thunbergii$) forests in 91 cities, counties (gun) and borough (gu), in seven provinces and three metropolitan cities during 2010. Black pine bast scale were found in 64.8% of cities or counties or borough (59) in 7 provinces and 3 metropolitan cities, and were distributed in all South Costal regions, Pohang in East Costal region and Boryeong in West Costal region. Chungcheongbukdo, Daejeon and Jeju did not have black pine bast scale. All the gu regions in Busan had black pine bast scale, of which the area with the highest prevalence was Haenam in Jeollanamdo (1.713 crawlers/0.785 $cm^2$). Songji-myeon had the highest occurrence rate (6.36 crawlers/0.785 $cm^2$) from the towns, township and dong. The density of black pine bast scale in twigs was highly correlated with percentage of the sample with scale (Correlation coefficacy=0.89).
3-deoxyglucosone에 의한 단백질의 modification
신동범,오상환,양융 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.1
pH 7.4, 37℃의 생리적 반응조건에서 HSA, ovalbumine, BSA, lysozyme, RNase A 및 IgG와 3-deoxyglucosone을 반응시키고 단백질의 중합현상과 아미노산 잔기의 손상상태를 연구하였다. 3-deoxyglucosone은 모든 단백질을 중합시킬 수 있으나 특히 lysozyme, RNase A 및 IgG를 현저하게 중합시켰다. 또한 Arginine잔기를 크게 손상시켰다. glucose에 의한 단백질의 중합과정은 glucose가 단백질의 아미노 그룹과의 반응으로 3-deoxyglucosone을 형성시키고, 이것이 단백질의 lysine 잔기와 arginine 잔기를 공격하여 cross link를 형성시키는 것으로 추정되었다. Human serum albumin, bovine serum albumin, ovalbumin, IgG, lysozyme and ribonuclease A were reacted with 3-deoxyglucosone under physiological conditions of 37℃ and pH 7.4, and polimerization of proteins and impairments of amino acid residues were investigated. Proteins tested, especially lysozyme, IgG and ribonuclease A, were polymerized significantly, and lysine residue and arginine residue, espectially arginine residue of proteins were impaired remarkably. Experimental results strongly suggested 3-deoxyglucosone, formed from proteins-glucose reaction system, was the cross-linker responsible for the polymerization of proteins.
申東範 제주대학교 1993 논문집 Vol.37 No.-
. The degradation products of L-ascorbic acid was examined by incubating it alone and in the presence of α-N-formal-L-Lysine(NFL) at pH 7.4(0.2M phosphate buffer) in the presence of oxygen, metal ion chelator, and in both the presence and absence of sodium cyanoborohydride. Among the large number of degradation products produced, threonic, oxalic, glyceric and glyoxylic acids, as well as threose were all identified by GLC-MS. Decarboxylation is also a feature of the degradation, with approximately 30% of the ascorbic acid undergoing degradation via this pathway. In the presence of NFL and cyanoborohydride ??-N-caboxymethyl-L-lysine(CML) was detected as a reaction product as well. Using GLC-MS as the detection method and ascorbic acid-1-?? as the reactant, only glyoxylic acid and CML were found to contain a ?? Label, indicating that, of the compounds identified above, only these latter two contain C-1 of ascorbic acid.
침자가 성장기 및 성숙기 웅성 흰쥐의 혈액성분에 미치는 영향
윤범철,이성동,류정록,최태암 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.2
Acupuncture, an over 2500 years old therapeutic method stemming from traditional oriental medicine, is nowadays wide open to modern medical science. With the widespread usage of acupuncture, the number of article about biomechanical or therapeutic effects of acupuncture from animal experiments and clinical trials is largely reported. In a previous study, we have reported that Joksamlee(ST36) acupuncture increases serum protein and glucose but decrease serum lipid and cholesterol. For further study, we was examined effects of Shenshu(B23) and Samumgyo(SP6) acupuncture stimulation on differential blood components in growing and adult male rate. 24 male Sprague-Dawley rats were randomly assigned into three groups; A1 (B23 and SP6 acupuncture in adult rats) group, A2 (B23 acupuncture in growing rats) group, and control group. The results obtained were as follows; 1. Body weight was significantly decreased in A1 and A2 groups. 2. Serum TP level was not changed, but albumin level was significantly increased in A2 group. 3. Serum glucose level was significantly increased in A1 group. 4. Serum TG level was significantly decreased in A2 group: PL level was significantly decreased in both A1 and A2 groups; FFA level was not changed. 5. Serum T.CHO, HDL-C and LDL-C levels were not changed in both A1 and A2. 6. Serum amylase activity was significantly increased in A1 group.
申東敏,長鳳愚,李揆文,金賢俊,金昌範,崔宗洙,崔鍾晥,李鍾珏,金圭碩,趙庚旭,金鍾聲,朴鍾振 平生體育硏究所 論文集 1987 平生體育硏究所 論文集 Vol.2 No.-
With a view to examining the habit and method in which old people maintain and improve their health, we consulted 551 men and 444 women who were over 65 years old, and concluded as follows: 1. Habit of eating It's been revealed that most of the old people keep a regular eating hour, control the quantity of meal or eat a little food, and prefer vegetables to meat. 2. Mental health Old people feel the necessity for mental health and most of them (about 80%) keep good mental health without becoming pessimistic. Their chief concenrs have turned out to be about their sons and daughters and their own health and finance. 3. Managing Good Health It has been revelaed that old people do not possess any special kind of secret to keep good health but taking plenty of rest, regular meal, and proper exercise, and many of them (about 60%) have been shown to eat invigorant food that they can easily get around their living area. 4. Physical Functions It has been revealed that many old people are conscious of some symptoms about their parts of body and about to percent of them suffers from neuralgia. Some measures like medical aid especially for old people need to be taken as soon as possible. As we have found that the health-keeping method of old people consists plenty of rest, proper exercise, and appropriate nutrition, we can conclude that the recreatonal and culture facilities and programs for keeping old people in good health should be developed. It is also necessary to study food that contribute to their health and develop ways to get rid of the stresses to which they are exposed in their daily life. Presides, it is natural that government found an administrative department to deal with these matters effectively.
혈액보존제 ACD-B,CPDA-1 및 SAG-M에 보존된 혈액의 혈액학적,생화학적 변화에 관한 연구
나동진,문희주,최범열 서울보건대학 1997 서울보건대학 부설 한국보건과학연구소 논문집 Vol.4 No.1
Backgrounds ; Study as to blood preservative solution is continuousely performed to prolong period of donor blood preservation which will cope with demand increase and autologous transfusion. In Korea, CPDA-1 solution, preserved 35 days, substituded ACD-B(21days), is currently used for anticoagulant preservative solution of blood. But SAG-M solution(42days) were widely used in other contries for longer preservation as RBCs additive solution. Authors compared the survival of RBCs preserved with ACD-B, CPDA-1 anticoagulant preservative solution, SAG-M RBCs additive solution to study changes of hematological and biochemical. Materials and Methods ; Blood were collected from 15 healthy male donors aged 22 to 23years, for three sorts of preservative solution bag made in home(Company Green Cross, Korea) at the same time. 5 of them were drawn into the blood bags containing ACD-B and another 5 into the CPDA-1 bags, the other 5 donors were collected into the blood bags with CPDA-1, plasma was removed by centrifugation serparation at 4,000rpm in 5minutes and replaced by 90 ml of SAG-M additive solution. All of blood bags were stored in blood bank refrigerator at 1 to 60℃. Samples were drawn at 1, 5, 10, 15, 20, 25, 30, 35 and 42days in each through blood bags tubing under sterile condition used SCD(Sterile Connection Device) so as to measure changes of hematological and biochemical. Results ; 1) A TP level is very important preservation index of hematological test. On 1 days, ATP levels of ACD-B anticoagulant preservative solution were measured by 4.85μmol/gHb, CPDA-1 5.05μmol/gHb, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) Plasma hemoglobin in bloods stored in ACD-B, CPDA-1 and SAG-M were 24.6mg/dl, 24.3mg/dl and 16.7mg /dl, respectively, on 1days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas pottasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of b, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) plasma hemoglobin in bloods stored in ACD-B. CPDA-1 and SAG-M were24.6mg/dl, 24.3mg/dl and 16.7mg/dl, respectively, on 1 days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas potasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of imported plasma in our country.
신동범,윤창훈,강경수 제주대학교 환경연구소 1997 환경연구논문집 : 제주대 Vol.5 No.-
We conducted the experiments to find out the appropriate gelling materials for the treatment of waste water by immobilizing microorganisms inside the gels. The gelling materials known to immobilize microorganisms or cells in them are polyacrylamide, polyvinylalcohol, sodium alginate, κ-carrageenan and chitosan. The gel strengths were determined by the breaking points of the pellet using a rheometer with reaction time. The polyacrylamide gels were retained their solidities and activities excellently compared with the other pellets of polyvinylalcohol, sodium alginate and chitosan with reaction time. It is considered that, in the other hand, the polyacrylamide gels are toxic to the single microorganisms and cells by free radicals during the formation of gels. We used activated sludge for the mmobilization, so it didn't matter the toxicity caused by free radicals. The profiles of waste water treatment by all the immobilized pellets using in this study were showed nearly similar patterns, but gel strength of all the gels except polyacrylamide pellets were getting weak and weak as reaction time goes by. It was concluded that the polyacrylamide gels are excellent for immobilization of activated sludge for waste water treatment, but in the case of immobilization of single microorganisms or cells, natural polysaccharides of sodium alginate, κ -carrageenan or chitosan are thought to be suitable, and it must be developed the techniques for elevating gel strength of nautral polysaccharides.