Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

코어안정화운동, 동방요법 및 병행처치가 골반변위에 따른 좌골신경통에 미치는 영향

김동호(Kim, Dong-Ho),김상현(Kim, Sang-Hyun),김성남(Kim, Sung-Nam),김재철(Kim, Jae-Cheol) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.4

The purpose of this study was to confirm the effect of core stability exercise, dong-bang therapy, and combined treatment on reducing pain, pelvic correction and stress hormone concentration in patients suffering from sciatic neuralgia due to pelvic displacement. Middle aged(40~50age) suffering from sciatic neuralgia over 6 months were operated with core stability exercise, dong-bang therapy, and combined treatment 3 times a week for 12 weeks. VAS and X-ray was performed to collect pain scales and pelvic displacement data and cortisol and epinephrine concentration in blood were tested. 1. pain scale rate was reduced in all groups. especially, dong-bang therapy group and combined treatment group showed more directive pain reduce. 2. pelvic displacement improvement was not significant in core stability exercise group, and it was significant in dong-bang therapy group and combined treatment group. no significant difference was shown between dong-bang therapy group and combined treatment group. however, combined treatment group showed higher correction effect. 3. concentration of stress hormone was significantly increased in all groups after the first operation. after 12 weeks concentration level was reduced to same level before treatment. Dong-bang therapy and combined treatment improve pelvic displacement, and are effective treatments on reducing sciatic neuralgia due to pelvic displacement. combined treatment of dong-bang treatment and core stability exercise is considered an effective program of correction for pelvic displacement and maintenance.

족과관절염좌 환자에 대한 동씨침법과 일반침법의 효과에 대한 비교 연구

안호진,정동화,황규선,윤기붕,김태우,문장혁,백종엽,이상무,Ahn, Ho-jin,Jeong, Dong-hwa,Hwang, Kyu-seon,Yoon, Ki-bung,Kim, Tae-woo,Moon, Jang-huyk,Baek, Jong-yeob,Lee, Sang-moo 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1

Objective: The purpose of this study is to compare the effects of Dong-si acupuncture therapy and General acupuncture therapy for the patients with acute ankle sprain. Methods : This study has been carried out for 60 cases of ankle sprain patients who have visited Dong-Seo Oriental medical Hospital from May 1, 2002 to September 30, 2002. We have treated 30 cases of them by Dong-si acupuncture therapy and the other 30 cases by General acupuncture therapy. And we have compared those two group. Results : 1. There was no significant difference at the treatment period and the number of treatment times in comparing two groups. 2. The number of treatment times for good effect is that : Dong-si took $1.57{\pm}0.85$ times and General acupuncture therapy took $2.15{\pm}0.96$ times. And we have found that the effect of Dong-si acupuncture therapy is faster than the other.

식민지 시기 신춘문예 제도와 작문 교육 : 『동아일보』를 중심으로

손동호(Son, Dong-Ho) 한국문학회 2021 韓國文學論叢 Vol.87 No.-

『동아일보』의 신춘문예는 신진 작가의 발굴을 내세우며 등장하였다. 이는 독자 참여 제도의 시행 목적이 ‘독자’ 확보에서 ‘작가’ 확보로 변모했음을 의미한다. 하지만 아동작품은 ‘작가’ 확보와는 다른 목적에서 시행하였다. 『동아일보』는 신춘문예 시행 첫해부터 문예계, 부인계, 소년계 등으로 독자의 층위를 구분하여 작품을 모집하였다. 이는 여성과 아동이라는 새로운 독자층을 발굴하여 가정란과 아동란을 신설한 매체의 지면혁신 정책과 연계된 작업이자, 이전 시기 독자 참여 제도의 전통 계승에서 비롯된 결과였다. 문화주의를 표방하며 창간한 『동아일보』는 계몽의 차원에서 여성과 아동 독자를 호출하였다. 민족을 개량하여 문명화를 달성하기 위해 아동을 교육함으로써 근대적 주체를 양성하고자 한 것이다. 신문의 잠재적인 독자층을 확보하기 위해서나 문명화를 추진하기 위한 아동 교육의 필요에 의해서나 아동을 대상으로 한 문장 교육은 중요한 과제였다. 작문은 이러한 복합적인 배경에서 선택되어, 신춘문예의 모집부문에 포함될 수 있었다. 신춘문예 작문 부문 당선작은 자연 묘사 및 아동의 일상을 주로 다루었다. 하지만 작문은 단순히 자연을 묘사하거나 사건을 기록하는 데 그치지 않고 각 상황에서 촉발된 구체적인 감정을 드러냈다. 이를 통해 작문이 조선어 글쓰기를 통한 아동의 정서 함양을 지향했던 사실을 알 수 있다. 식민지 시기 총독부의 교육 정책은 조선어 교육을 폐지하는 것이었다. 이러한 상황에서 동아일보사는 브나로드 운동을 주관하고, 다양한 한글 관련 기획란을 마련하여 언문철자법의 보급에 앞장섰다. 그리고 아동들에게 읽을거리를 제공하는 한편 조선어로 된 작품을 모집, 발표하였다. 결국, 작문은 습작을 통한 조선어 글쓰기의 보급 및 아동 계몽을 목적으로 시행되었으며, 아동 계몽의 실질적인 내용은 정과 동정의 함양이었다. 신문의 지면개편에 관여하고 신춘문예를 주관한 이광수는 아동 교육에 관심이 많았다. 그는 근대적 사회를 형성하는 데 필요한 새로운 감정 규범으로 정과 동정을 제시하고, 그 실현 수단으로 작문을 활용하였다. 당선작의 내용을 분석한 결과, 제도 시행 초기 편집진의 이러한 의도가 신춘문예 시행동안 지속적으로 관철되었음을 확인하였다. The Spring Literary Contest of “Dong-A Ilbo” appeared with the excavation of young authors. However, works for children were implemented for a purpose different from discovering new authors. “Dong-A Ilbo” recruited works by dividing the layers of readers into literary, ladies, and boys from the beginning of the Spring Literary Contest. It was a work in connection with the media s paper innovation policy that created a family section and a children section by discovering a new readership of women and children. “Dong-A Ilbo”, which advocated culturalism, called women and children in the dimension of enlightenment. It aimed to cultivate a modern subject by educating children to improve the nation and achieve civilization. To secure a potential readership of newspapers, or because of the educational need to promote civilization, sentence education for children was an important task. The composition was conducted in this context. The winning work of the Spring Literary Contest mainly coped with the depiction of nature and the daily life of children. The composition did not stop at simply describing nature or recording events, but revealed specific emotions triggered in each situation. Through this, it can be seen that the composition was aimed at cultivating children s emotions through writing in Korean (Joseoneo). The educational policy of the governor-general during the colonial period was to abolish Korean language (Joseoneo) education. In this situation, Dong-A Ilbo took the lead in spreading the Korean word spelling by managing the Vnarod movement and preparing various Hangeul-related planning sections. In addition, while providing reading materials to children, it recruited and published works in Korean (Joseoneo). The composition was attempted at the dimension of teaching sentences for children to create a modern subject. Lee Gwang-su, who hosted the Spring Literary Contest, was very interested in education for children, and was also involved in reshuffling sections of the newspaper. He presented affection and sympathy as a new norm of emotion necessary to form a modern society, and used his writing as a means of realization.

백석 문학의 전체성에 대하여

최동호(Choi, Dong?ho) 한국비평문학회 2012 批評文學 Vol.- No.46

This paper aims to have a bird’s?eye view on Baek, Seok’s works who has reached the 100th anniversary and avoid the mistake of looking into the parts and not the whole. Paek, Seok was born at Jeong?ju, Pyeong?buk on July 1912 and lived a life of extreme ups and downs. He made his literary debut through annual spring literary contest of Chosun Daily newspaper on January 1930 when he was 19 and finished his publication of works with ‘Naruteo’(Ferry) on May 1962. After he went to a cooperative in Gwanpyong?ri, Samsoo?gun leaving the central literary circle on January 1959, he lived as a shepherd for more than 30 years of his later part of life. Having been brought into sudden prominence in the literary circle with his poem book Saseum(Deer) in 1936, Paek, Seok kept his literary reputation as a writer of juvenile stories by publishing fairy poem book Jipgene Ne Hyongje(Four Brothers of the Hermit Crabs, 1957) in the North Korea. However, he had to focus on children’s literature without the freedom of writing and he could not be reinstated to Pyongyang after he had received the ‘Red Letter’ in 1959. Referred as a genius poet, however, his life was ended tragically without beingable to write what he wanted to and criticized for his lack of loyalty and ‘vestige of bourgeois.’ Total unearthed poems of Paek, Seok are 148 including recent ones discovered by Song, Joon. More of his unearthed works will be included later. His proses are total 44 with the variety of novel, essay, review, political comment, translation, etc. He focused more on translation in the North Korea and they are 197 works of translated modern poems and 11 works of fairy poems. He translated 4 long novels including Silent Don 1, Silent Don 2 and 1 collected short novel and 2 short novels. Silent Don recently discovered with the help of professor Yoon, Hae?yeon is more than 1,000 pages and has an importance as the first direct translation fro Russian into Korean by Bake, Seok. More works of him will be unearthed as time goes on. However, with those newly unearthed works, the study on Baek, Seok’s literature has already reached a new phase. Now researchers have to include those works written in the North Korea. Unearthing the texts of important poets such as Baek, Seok is very meaningful for the study of modern Korean poetry. Considering the process of unearthing his works until now, more of his translated works will be found. However, his own creation has less possibility of being included in the list. Though there will be the difficulties such as the division of Korea into south and north, researchers should be able to get over them to continue the study of Baek, Seok.

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

사례보고 : 하수오 복용 후 발생한 재발성 독성 간염 1예

배상훈 ( Sang Hoon Bae ),김동현 ( Dong Hyun Kim ),배영석 ( Young Seok Bae ),이광재 ( Kwang Jae Lee ),김동완 ( Dong Wan Kim ),윤정빈 ( Jeoung Bin Yoon ),홍준호 ( Joon Ho Hong ),김상현 ( Sang Hyun Kim ) 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.2

Toxic hepatitis has been reported as a major cause of acute hepatitis, but its potential induction by herbal remedies and/or health foods is usually neglected. We experienced a case of toxic hepatitis associated with Polygoni multiflori, a Chinese herb commonly known as Ho-Shou-Wu. A 54-year-old woman consumed Ho-Shou-Wu for 1 month, after which she experienced fatigue and overall weakness. A diagnosis of toxic hepatitis was made based on her clinical history, the findings for viral markers and other laboratory data, and ultrasonography. Her condition improved considerably after she stopped taking Ho-Shou-Wu. However, she resumed taking Ho-Shou-Wu immediately after discharge from hospital, which aggravated her symptoms and liver function. She was immediately readmitted and stopped taking Ho-Shou-Wu. Her relapse into hepatitis immediate after resuming consumption of the herb is strongly indicative of the validity of Koch`s postulate in this case.

Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

삼국시대 신라의 동해안 제해권 확보의 의미

김호동(Ho Dong Kim) 대구사학회 2001 대구사학 Vol.65 No.1

Taking a look at the rock-paintings, Suk Tal-Hae myth, Wooro legend, we can confirm that Shilla had taken a lot of interest in territorial expansion to the east coast since its early age, based on its marine-fishery culture. And then Siljik-Joo in Sam-chuk, Aseulla-Joo in Kang-reung, Sokyung in Asichon were established along with Yi-sa-boo`s conquest of Woosan-Kook during the reign of King JiJeung. Through such activities, Shilla succeeded in securing the command of the east sea(Dong-hae) and the east coast. Shilla`s expansion to the east coast, ranging to Wool-jin, Sam-chuk, Kang-reung, during the reign of King Ji-jeung was probably accomplished in the form of feint operation, not only through the land traffic route but also through the coastal route by marine forces which had been maintained since Suk Tal-Hae` age from rock-paintings. Afterwards, Shilla, which captured those areas, probably launched the conquest of Woolreung-do from Wool-jin. Sam-chuk. These activities made it possible for Shilla to seize t:he rulling power over the east coast, east sea(Dong-hae) and finally make such rapid progress as dispatching Koanjoo(governor), subjecting Keumkwan-Kaya in the reign of King Bupheung as well as capturing the Han-river, the Nakdong-river basin, establishing Biyeulhole-Joo, conquering Kaya, setting up a number of stone monuments, which are now called King-Jinheung-Soonsoo-Bi, in Bookhan mountain, Hwangchoryung in Heungnam province, Maoonryung in Yione under the reign of King Jin-heung. Considerable amount of attention should be paid to the fact that Shilla`s feint operation through the went coast and the east coast against KoKooryu was possible by its taking economic power in the east coast, following its expansion to the east coast and conquest of Woosan-kook. In Shilla`s such a success, Woosan-kook`s coorporation played crucial role. Therefore, Woosan-kook was not so much destroyed as enjoying great prosperities with a relative stability, which was attributed to Shilla`s securing the command of the sea.