저산소에 노출된 넙치(Paralichthys olivaceus)의 호흡대사와 혈액의 화학적 변화

한지도 ( Ji Do Han ),김흥윤 ( Heung Yun Kim ) 한국수산과학회 2016 한국수산과학회지 Vol.49 No.1

This experiment investigated changes in metabolic rate (MO2), critical oxygen saturation (Scrit), and blood chemistry of olive flounder Paralichthys olivaceus exposed to progressive hypoxia and returned to normoxic water at 20°C. The normoxic standard metabolic rate (SMR) and routine metabolic rate (RMR) were 69.5-83.9 and 70.2-156.4 mg O2 kg-1h-1, respectively based on fish weight. Scrit was 31.0% dissolved oxygen (DO) at 20°C. After returning the fish to 70% DO following exposure to hypoxia (20% DO), MO2 increased two-fold compared to the normoxic SMR and then decreased into the range of the RMR with time. Blood PO2 and plasma lactate decreased significantly after exposure to hypoxia (20% DO) and then increased as ambient oxygen saturation decreased. Cortisol levels increased as ambient oxygen saturation decreased, but the levels decreased rapidly in the range of the normoxic control when the fish were returned to ambient water with 70% DO. Plasma glucose levels increased when the fish were returned to normoxic water after exposure to a progressively more hypoxic condition.

백서 두개골에 형성된 골 결손부 크기에 따른 골 치유과정에 대한 조직학적 연구

윤도철,김경욱,임창준,김종여 단국대학교 치의학연구소 1994 논문집 Vol.6 No.1

The author made calvarial defects of different sizes in rat to study the effect of defect size on healing process. Experimental animals were sacrified at 3 days, 1 week, 2, 4, 6 weeks. The specimens were examed under light microscope with Hematoxlin-Eosin and modified Gomori's trichrome stain, and under fluorescent microscope after glinding the specimens. The results were as follows. 1. 4㎜-diameter calvarial defects healed about 70% after 6 weeks. 2. Lamellated bone trabecules were seen in 6㎜-diameter calvarial defects after 4 weeks, and the small specules of bone and marrows were observed after 6 weeks. 3. 10㎜-diameters calvarial defects healed about 50% and incomplete interconnection of the lamellated bone trabecules were observed after 6 weeks. 4. Under fluorescent microscope, about 4㎜-long bone regeneration could be seen in most of the defects.

Lipomyces starkeyi KSM 22 Glucanhydrolase 함유 용액의 치은 섬유아세표에 대한 영향

윤현정,정현주,김옥수,김도만 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.3

A novel glucanhydrolase from a mutant of Lipomyces starkeyi KSM 22 has additional amylase activity besides mutanolytic activity and has been suggested as promising anti-plaque agent. It has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 glucanllydrolase are desirable for its application as a dental plaque control agent. In human experimental gingivitis model and 6month clinical trial. mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effect was negligible. This study was aimed to evaluate the cytotoxic effect of Lipomyces starkeyi KSM 22 glucanhydrolase on human gingival fibroblasts. Primary culture of human gingival fibroblasts at the 4th to 6th passages were used. Glucanhydrolase solution was made from lyophilized glucanhydrolase powder from a mutant of Lipomyces starkeyi KSM 22 solved in PBS and added to DMEM to the final concentration of 0.5, 1, and 2unit. Cells were exposed to glucanhydrolase solution or 0.1% chlorhexidine and the cells cultured in DMEM with 10% FBS and 1% antibiotics as control. After exposure, the morphological change, cell attachmcnt. and cell activity by MTT assay were evaluated in 0.5, 1.5, 3, 6, 24 hours after treatment. The cell proliferation and cell activity was also evaluated at 2 and 7days after Iminute exposure, twice a day. The cell morphology was similar between the Lipomyces starkeyi KSM 22 glucanllydrolase groups and control group during the incubation periods. while most fibroblasts remained as round cell regardless of incubation time in the chlorhexidine group. The numbers of the attached cells in the glucanhydrolase groups were comparable to that of control and significantly higher than the chlorhexidine group. The numbers of the proliferated cells in the glucanhydrolase groups at 7 days of incubation were comparable to the control group and higher than the chlorhexidine group. The cell activity in glucanhydrolase groups paralleled with the increased cell number by attachment and proliferation. According to these results, Lipomyces starkeyi KSM 22 glucanhydrolase has little harmful effect on attachment and proliferation of human gingival fibroblasts, in contrast to 0.1% chlorhexidine which was cytotoxic to human gingival fibroblasts. Therefore this glucanhydrolase preparation is considered as a safe and promising agent for new mouthwash formula in the near future.

임신과 동반된 외음부 상피내 종양 1례

김윤숙,김미영,전섭,김종수,최승도,선우재근,배동한,오미혜 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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6-(3,4-디클로로페닐)아미노-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가

윤여표,김동현,이병무,허문영,정해문,강혜영,최정아,김도희,유충규 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

6-(3.4-Dichlorophenyl)amino-7-chloro-5.8-quinolinedione (RCK50) was tested for antifungal activities in mice systemically infected with Candida albicans. The therapeutic potential of RCK50 was also assessed in comparison with ketoconazole. RCK50 had ED_50 0.22±0.01 ㎎/㎏. Ketoconazole as a positive control had ED_50 6.00±1.70 ㎎/㎏. Intraperitoneally administered RCK50 at the ED_50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver. And administered RCK50 at the ED_50 for 14 days improved survival rates. The genotoxicities of RCK50 had been evaluated. RCK50 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. RCK50 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK50 has no genotoxic potential under these experimental conditions. Acute oral toxicity studies of RCK50 were carried out in ICR mice of both sexes. RCK50 did not show acute oral toxicities and LD_50 values were over 2.850 ㎎/㎏ in ICR mice.

관광 여행 가이드 매칭앱 설계

김도윤,박형민,오상태,이성욱 韓國交通大學校 2022 한국교통대학교 논문집 Vol.57 No.-

After a period of tourism stagnation due to the corona virus epidemic, Korea is preparing to lift self-isolation and PCR results. Accordingly, we designed a platform application that can connect consumers and suppliers of travel guides that can respond to the influx of domestic and foreign tourists. DB design and client design were made using NoSQL-based Firebase server, Android Studio, and Java language. The suggestion app provides location-based tourist recommendation and tourist information output using GPS, and tour guide matching service using user information.

노래부르기 과업이 브로카 실어증자의 읽기와 조음 능력에 미치는 영향

윤혜연,권도하 대구대학교 특수교육재활과학연구소 2012 再活科學硏究 Vol.30 No.1

이 연구는 가사보며 노래부르기 과업으로 브로카 실어증자의 읽기와 조음능력을 향상 시키고자 하였다. 3명의 브로카 실어증 대상자로 하였으며 연구 방법은 사전-사후 검사를 사용하였다. 이 연구에서 사용된 노래부르기 과업은 3단계로 구성되어있다. 노래는 대상자에게 있어 익숙한 노래 5곡을 선정하였다. 1단계는 제공된 MP3파일로 노래를 들으며 따라부르기를 한다. 2단계는 문자단서로 제공된 노래가사를 한 음절씩 짚으며 따라 부르기를 하고, 3단계에서는 음을 점진적으로 제거하며 가사 읽기를 시도한다. 이에 치료실외에서도 하루에 3회씩 연습할 수 있도록 지도하였다. 그 결과 노래부르기 과업은 읽기, 조음능력 뿐만 아니라 따라말하기, 이름대기 등 전반적인 언어능력에 있어 향상을 보였다. The purpose of this research was to explore the effect of singing while reading the lyrics had on the reading and articulation skills of people with Broca’s aphasia. The study consisted of three subjects with Broca’s aphasia and the results were analysed by pre-to-post analysis. Thirdly, through the implementation of the task of singing, linguistic abilities, such as reading, intonation, word-repeating and naming skills were all improved. The five songs that were chosen were songs that the participants were familiar with. The first stag involved participants singing along to the songs that were given to them as an MP3 file. The second stage involved participants looking at the hard copy of the lyrics and then breaking up each syllable while singing out loud and the third stage involved gradually omitting the tone from the songs and getting participants to only read the lyrics out loud. Participants were advised to repeat these three steps three times a day outside of the treatment room. The results of this study are as follows: Firstly, there was an improvement in the reading skills following singing tasks. Secondly, through implementing the task of singing there was an articulation in the clarity of intonation. Thirdly, through the implementation of the task of singing, improvements in reading, the ability to intonate, repeating words and naming skills were evident; as well as an overall improvement to the linguistic ability of the people.

레싱 : 문학을 통한 계몽 Dramen 희곡

윤도중 한국뷔히너학회 2004 뷔히너와 현대문학 Vol.23 No.-

Im Gegensatz zu Epigrammen und Fabeln, die Lessing zum Medium macht, um das Selbstdenken der Leser zu u¨ben und zu fdrdern, ha¨lt er das Drama, insbesondere die Trago¨die fu¨r die Gattung, die durch Appell an die Empfindung der Zuschauer oder der Leser die Leidenschaften erregt und die Empfindungsfa¨higkeit u¨bt. Nach seinem Trago¨dienkonzept ist der Zweck der Trago¨die die moralische Besserung, die durch die Entwicklung der Empfindungsfa¨higkeit mo¨glich ist. Er macht das Mitleid zum Zentralaffekt der Trago¨die, weil er der u¨berzeugung ist, dass es die Basis des guten Menschen darstellt. Ihm kommt es darauf an, dass die Zuschauer das Mitleid und die Humanita¨t, die im Theater geu¨bt werden, im wirklichen Leben praktizieren. u¨bt das Trauerspiel die Mitleidsfa¨higkeit, so liegt das Nutzen des Lustspiels "in der u¨bung unserer Fa¨higkeit das La¨cherliche zu bemerken". Da unsere Hundlungen auch in die Kategorie des La¨cherlichen geho¨ren, ist das davon Erregte nicht das kalte Verlachen, sondern das aus dem Versta¨ndnis der menschlichen Beschra¨nktheit fliessende warme Lachen. Das Lustspiel bessert, indem es durch solches Lachen die menschliche Unvollkommenheit bewusst macht. Die deutschen Aufkla¨rer streben eine Gesellschaft an, in der alle Mitglieder miteinunder glu¨cklich in Toleranz leben ko¨nnen und glauben, dass sich eine solche Gesellschaft durch Erziehung verwirklichen la¨sst. Das neue Dramenmodell von Lessing zielt darauf ab, die Bu¨rger durch Fo¨rderung ihrer Selbstdenk- und Empfindungsfa¨higkeit zu aktiver Humanita¨t anzuregen und damit zur Herausbildung einer von ihnen gepra¨gten Gesellshchaft beizutragen. Wa¨hrend Lessings fru¨he Lustspiele dazu neigen, durch Appell an die Vernunft das Selbstdenkvermo¨gen der Zuschauer oder der Leser zu fo¨rdern, beabsichtigen seine Trauerspiele, menschliche Verhaltensweisen u¨berdie Empfindungsfa¨higkeit zu entwickeln. Sie zeigen, dass das Mitleid und die Menschenliebe zuna¨chst im privaten Bereich der Familie erwachsen, aber weisen ferner darauf hin, dass diese Menschlichkeit u¨ber gesellige Beziehungen zu gleichgesinnten Menschen in die o¨ffentliche Spha¨re der Gesellschaft hineinwirken muss, wenn ein humaneres und glu¨cklicheres Zusammenleben der Menschen verwirklicht werden soll, dessen Vision Minna von Barnhelm und Nathan der Weise zeigen.

문학을 통한 계몽 : 경구시와 우화 das Epigramm und die Fabel

윤도중 한국괴테학회 2003 괴테연구 Vol.0 No.15

Lessing ha¨lt die Zweigliedrigkeit fu¨r die ideale Struktur des Epigramms. Die ,,Auflo¨sung", die die ,,Erwartung" u¨berraschend befriedigt, bietet dem Dichter Gelegenheit, Scharfsinn, Einfall, Witz zu u¨ben und darzulegen sowie sinnreiche und pra¨gnante Formulierungen zu erproben. Um die Pointierung nachzuvollziehen, muss sich der Leser Gedanken machen. Lessing stellt die Mu¨he des Nachdenkens vor die Freude der Einsicht, die das Epigramm vermittelt. ,,Der moralische Lehrsatz", den Lessing als Endzweck der Fabel ansieht, ist der Erfahrungssatz. Die Fabel sol auf die individuellen und sozialen Verhaltensnormen einwirken. Lessing meint, dass die Fabel nicht dazu dient, Lebensregeln zu vermitteln, sondern den Leser anzuregen, in den gegebenen Situationen ada¨quate Verhaltensweisen zu finden. Und er sieht den ,,heuristischen" Nutzen als den besonderen der Fabeln in den Schulen an. Das Vergnu¨gen der Fabel kommt nicht aus der lustigen Geschichte oder den witzigen Formulierungen. Das Lesevergnu¨gen ist identisch mit der Denkanstrengung bei und nach der Lektu¨re. Die Analyse Lessingscher Theorie u¨ber das Epigramm und die Fabel besta¨tigt, dass seine Schriften die Aufkla¨rung des Lesers zum selbsta¨ndigen Denken bezwecken. Da Lessing auf die Aufkla¨rung des Lesers abzielt, ist er darauf bedacht, das zu schreiben, was der Leser liest und versteht. Und er pra¨sentiert dem Leser nicht das Denkergebnis, sondern bezieht ihn in die Gedankenga¨nge ein, um ihn zum Denken anzuregen. Wie die Fabel Die Erscheinung zeigt, ha¨lt er den selbsta¨ndig denkenden und die Lehre ziehenden Leser fu¨r den idealen. Er strebt danach, sein Publikum zu diesem idealen Leser heranzubilden, na¨mlich zum mu¨ndigen Menschen, der zum Aufbau einer humanisierten Gemeinschaft beitragen ko¨nnte. Diese Bemu¨hungen setzt er als Dramatiker und Kritiker fort.

산전 태아사망 환자에서의 Mycoplasma hominis와 Virdans streptococcus감염 1예

김윤숙,문성택,전섭,김종수,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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