Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kB and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

( Jae Ho Choi ),( Sun Woo Jin ),( Eun Hee Han ),( Bong Hwan Park ),( Hyung Gyun Kim ),( Tilak Khanal ),( Yong Pil Hwang ),( Minh Truong Do ),( Hyun Sun Lee ),( Young Chul Chung ),( Hee Suk Kim ),( Tae 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-α, IFN-γ, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-α/IFN-γ-induced TARC expression through the suppression of NF-κB and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms.ⓒ2014 Elsevler Gmbh. All rights reserved.

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

실험연구 : 마우스에서 Procaine 투여에 의한 Cisplatin 신독성의 완화 기전

안도환 ( Do Whan Ahn ),김상래 ( Sang Rae Kim ),하동호 ( Dong Ho Ha ),김세환 ( Se Hwan Kim ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.52 No.3

Background: Procaine binds to DNA and reduces cisplatin nephrotoxicity, but the mechanism is poorly understood. We explored whether procaine amelioration of cisplatin nephrotoxicity was related to down-and/or up-regulation of inflammatory response gene tumor necrosis factor-α (TNF-α), oxidative stress indicator gene heme oxygenase-1 (HO-1) or cell cycle inhibitor gene p21. Methods: Cisplatin and procaine were intraperitoneally injected to mice at a single dosage of 16 and 80 mg/kg, respectively. Renal evaluation was performed 72 hours after cisplatin administration. The expression of transcripts and proteins was analyzed using real time RT-PCR and Western blot, respectively. Results: Procaine treatment moderately attenuated necrotic changes of renal proximal tubules and increases in BUN and creatinine concentration by cisplatin administration. Kidney platinum level between the cisplatin (cis) group and the cisplatin + procaine (CisPro) group was not different. Although the level of TNF-α mRNA increased 4-fold higher in the Cis group than in the control, this increase was not attenuated by procaine treatment. Gene expression of p21 and HO-1 was elevated 175 and 4-times higher in the Cis group than in the control, respectively. But their expression was no further elevated, rather significantly reduced in the CisPro group compared to the Cis group. Protein abundance of p21 and HO-1 was paralleled by their respective mRNA expression. Conclusions: Procaine amelioration of cisplatin nephrotoxicity is likely to be achieved through processes other than the regulation of TNF-α, HO-1 or p21 gene expression. (Korean J Anesthesiol 2007; 52: 318~27)

비온의 연구에 나타난 시간성의 정신분석적 의미 : 버지니아 울프의 소설 『등대로』를 중심으로

이은도(Lee, Eun?Do),이준호(Lee, Joon?Ho) 한국현대정신분석학회 2020 현대정신분석 Vol.22 No.1

“시간이 흐른다”는 것은 자연 속에 존재하는 당연한 현상이므로 그 정신분석적 의미는 간과되기 쉽지만 비온은 그 의미에 대해 논한 바 있다. 비온의 연구에서 시간성은 사고의 발달 과정에서 나타난다. 시간의 측정은 좌절을 견딜 수 있는 유아에게 가능하며, 부재하는 젖가슴과 젖가슴 사이의 간격 측정과 “무의식적 깨어 생각하기”의 과정을 거쳐 유아의 내면에 시간의 흐름에 대한 관념이 생겨난다. 본 논문에서는 시간의 흐름에 대한 관념은 사고의 발달이 가능한 표지로서 기능할 수 있다고 보았고, 사고의 방향성이 가진 시간적 측면에 따라 시간을 K시간과 O시간으로 나누었다. 시간에 대한 인식의 실패는 O로의 변형에 필연적으로 수반되는 시간의 분열에서 생겨날 수 있으며, O로의 변형을 시도하였으나 좌절을 견디지 못하여 –K로 도피하였을 경우에도 생겨난다. 이때 K시간을 방어로서 사용할 수 있다. 이러한 시간에 대한 인식의 실패가 있는 경우 시간에 대한 특정한 증상으로 발현될 수 있다. 본 논문에서는 이러한 사례로 버지니아 울프의 작가 일기 「로드멜 1926」을 선정하였다. 「로드멜 1926」은 버지니아 울프의 자전적 소설인 『등대로』를 집필하던 당시의 일기로, 소설 속에는 버지니아 울프의 알파 기능, O에 직면하고 이를 변형하려고 시도하는 모습, 좌절을 견디지 못하여 –K로 도피하는 모습, 그 과정에서 나타난 시간의 분열과 멸절의 양상이 잘 드러나 있다. 「로드멜 1926」에 나타난 시간적 측면의 증상은 버지니아 울프가 소설 속에서 보여준 시간에 대한 인식의 실패가 현실로 발현된 사례로 볼 수 있을 것이다. “Time flows” is a natural phenomenon. But it’s psychoanalytic implications may not be obvious. In Bion’s study, measurement of time becomes possible for infants who can endure frustration, and through the process of “Unconscious wakeful thinking”, the notion on continuity of time is created in the infant’s mind. Time is classified as K time and O time according to the thought’s direction upon the aspect of time in this article. Failure of recognition of time can be generated from the splitting of time that is inevitably accompanied with the transformation of O, and it is generated when one tries to transform into O but cannot endure the frustration so as to escape into –K. In this case, K time can be used as a defense. When failure of recognition of time occurs, it can manifest as a certain symptom related to time. Virginia Woolf’s diary, “Rodmell 1926” would be this case. “Rodmell 1926” is Virginia Woolf’s diary when she wrote her autobiographical novel, To the Lighthouse. In the novel, her alpha function, the confrontation and attempt at transformation of O, the escape to –K due to intolerance of frustration, and splitting of time and annihilation were well illustrated. The symptoms of time that appeared in “Rodmell 1926” can be considered as the case of failure of recognition of time that Virginia Woolf wrote about in her novel, which was expressed in reality.

중 · 근세 척법 어휘의 단위 의미 분석

이광호(Lee, Kwang-Ho),도희금(Do, Hee-Geum) 한국어문학회 2018 語文學 Vol.0 No.141

경상북도의회와 의원의 의정활동에 대한 의원과 공무원의 인식에 관한 SURVEY 조사연구

이도선,차승호 동양대학교 1998 동양대학교 논문집 Vol.4 No.1

The purpose of this research is to survey and to compare the perception of local councilors and public officials toward local council and councilors in Kyungsangbuk-do provincial government. In this survey, we divided the conception of perception into five category which is composed of twelve measurable variables. The first category is the perception toward councilors; the 2nd is toward the function of council; the 3rd is toward councilors' knowledge about the administration performed by local government; the 4th is toward the relation between the executive & council; the 5th is toward development of local autonomy. This category is too broad its meaning to measure, so divided into twelve variables. We analyzed survey data with a frequency analysis, crosstabulations, ANOVA in SPSSWIN program, and discovered that there's significant gap between councilors and public officials in these five category. With analysis, we concluded that the different functions and role caused to gap in perception.

수중기지국 기반 수중통신망 구축 및 성능 시험

김도훈(Kim Do Hoon),채광영(Chae Kwang Young),고만재(Go Man Jae),정해지(Jeong Hae Ji),유호동(Liu Hao Dong),조용호(Cho Young Ho) 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.2

본 논문에서는 수중음향통신을 활용하여 CTD센서와 DO센서를 통해 수중환경데이터를 측정하고, 측정된 값을 육상에서 확인할 수 있도록 하는 실험을 진행하였다. 본 실험은 남해 실해역에서 20년 12월 12일부터 20일까지 진행되었으며, 구조물은 해수면에 해상부이 1대, 해저면에 수중기지국 2대, 센서노드 10대를 설치하였다. 모든 구조물에는 송신을 위한 트랜스듀서와 수신을 위한 하이드로폰이 장착된다. 해상부이는 해저면에 설치된 수중기지국과 센서노드에서 수중환경데이터를 수신한 후 육상 서버와 통신하여 패킷을 전송한다. 데이터는 1시간 단위로 전송되도록 설정하였으며 실험결과, 패킷 성공률은 약91.20%인 것을 확인하였다.

혈액투석 환자의 가성 고칼륨혈증

최창렬,조형도,강태영,이준수,한호,정청일,이동규,유준호,한상웅,김호중 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

응급센터 입원환자의 통증처치에 대한 응급의학적 고찰

도병수,김현정,정준영,도호석,이창현,이삼범 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Study objective: To recognize importance of pain management in ED and properly manage the ED patients complaining pain by analyzing the intervals between arrival time in ED and pain medicine starting time, the types and the administered routes of pain medicine. Design: Retrospective review of ED charts Setting: University teaching hospital Participants: The patients had admitted in ED of Yeungnam University Hospital with a chief complaint of acute abdominal pain or pain due to bone fracture between June 1, 1995 and December 31, 1995. Results: The study group consisted 256 patients, 161 of them female. ED administrations of analgesics were 103 cases, 17 of them administered more than two times. The average time interval between arrival time and medication time was 169.02±120.25 minutes. Each types of perceived analgesics were antispasmodics 55 cases(53.40%), NSAID 26 cases(25.24%) and opioid 19 cases(18.45%). Every analgesics were administered through intramuscular routes, except only 3 cases. Violences of patients or relatives due to delayed pain control were occured 32 cases, 5 cases of them self-discharged and looked for other hospitals. Conclusion: Emergency physicians including interns and residents delayed management so long and ordered inappropriate administration routes and types of analgesics. So we thought proper protocols about pain management in ED were established.

한국산 독말풀에 대한연구(Ⅰ) : HPLC에 의한 Hyoscyamine 과 Scopolamine의 정량

도경삼,신민교,송호준,선중기,손인경,백승화 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3

A new method for quantitative determination of Hyoscyamine and Scopolamine in Datura stramonium L. by High Performance Liquid Chromatography(HPLC) was established. A reversed-phase system with a μ Bondapak C18 column using methanol in 0.2% aqueous phosphoric acid(40:60) with PIC A as a mobile phase was developed. Hyoscyamine and Scopolamine were detected at 254nm and the analysis was successfully carried out within 20min.