Analysis of Equivalent Pressure for Circular Prestressing Applied on Concrete Tube Structures

Devkota, Prakash(프러카시 데브코타),Park, Joonam(박주남) 한국콘크리트학회 2022 한국콘크리트학회 학술대회 논문집 Vol.34 No.1

Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases

Devkota, Sushil,Jeong, Hyobin,Kim, Yunmi,Ali, Muhammad,Roh, Jae-il,Hwang, Daehee,Lee, Han-Woong TaylorFrancis 2016 Autophagy Vol.12 No.11

<P><B>ABSTRACT</B></P><P>Historically, the ubiquitin-proteasome system (UPS) and autophagy pathways were believed to be independent; however, recent data indicate that these pathways engage in crosstalk. To date, the players mediating this crosstalk have been elusive. Here, we show experimentally that EI24 (EI24, autophagy associated transmembrane protein), a key component of basal macroautophagy/autophagy, degrades 14 physiologically important E3 ligases with a RING (really interesting new gene) domain, whereas 5 other ligases were not degraded. Based on the degradation results, we built a statistical model that predicts the RING E3 ligases targeted by EI24 using partial least squares discriminant analysis. Of 381 RING E3 ligases examined computationally, our model predicted 161 EI24 targets. Those targets are primarily involved in transcription, proteolysis, cellular bioenergetics, and apoptosis and regulated by TP53 and MTOR signaling. Collectively, our work demonstrates that EI24 is an essential player in UPS-autophagy crosstalk via degradation of RING E3 ligases. These results indicate a paradigm shift regarding the fate of E3 ligases.</P>

Offshore Winds in the Gulf of Thailand: Climatology, Wind Energy Potential, Stochastic Persistence, Tropical Cyclone Influence, and Teleconnection

Bikash Devkota,Kasemsan Manomaiphiboon,Piyatida Trinuruk,Hoang Thi Trang,Carina Paton 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.3

This study investigated various characteristics of offshore winds at 100 m over the Gulf of Thailand (GOT) including its vicinity using JRA–55 reanalysis data during 1959–2018. The hierarchical clustering used yields six homogeneous clusters of wind speed in the GOT. Wind speed shows double peaks in December–January and July–August, reflecting a signature of the two regional monsoons. In the wet season, the upper region of the GOT tends to receive southwest monsoonal winds more directly while, in the dry season, the lower region is more impacted by the northeast monsoonal winds. The degree of diurnal variation in wind speed is little and much lower than that of seasonality. Trends in wind speed are found to be of less concern because their magnitudes are relatively small and the areas close to the coasts do not show any significant change. A stochastic concept was proposed to account for the persistence and intermittency of wind speed using a transition matrix and a turbine power curve, finding the degree of persistence of utilizable wind speed dominates in every case of region and season. Based on the developed threshold of 1,000 km as the distance from the tropical cyclone center, the upper subregions are the most impacted by tropical cyclones, especially during October − November. However, tropical cyclones do not significantly affect wind speed over the long term. Using the principal component and correlation analyses, it was found that the interannual variability of wet–season winds in the upper region is mainly associated with ENSO and PDO while dry–season winds in the lower region is mainly coupled with ENSO, in fair agreement with the wavelet coherence results.

Current Status of Production and Trade of Nepalese Tea

Sakuntala Devkota(사쿤타라 데브코타),Young-Goo Park(박용구),Tae-Kyun Kim(김태균),Jun-Ho Yeo(여준호) 한국차학회 2008 한국차학회지 Vol.14 No.3

본 총설은 네팔차의 생산과 수출 현황에 대해 고찰한 것이다. 네팔차는 영국동인도회사에 의해 인도에서 도입되었다. 145년의 차 재배역사를 가지고 있으며 네팔 차 시장을 형성해 왔다. 1883년 네팔 동부지역에서 두개의 차회사가 설립된 이래 약 80여 년간 정부의 도움을 받지 못했다. 1966년 정부에서 차 회사 설립을 지원 해주기 시작하면서 단기간 안에 대여섯개에 달하는 대형차 회사가 설립되었다. 이어 네팔정부에서는 1990년대에 들어와 차생산협동조합체제를 도입하고 은행 융자를 쉽게 받도록 조치하여 개인들이 많은 차 생산공장을 설립하도록 지원해 주었다. 이러한 정부지원 정책에 힘을 입어 1994년부터 2007년 사이에 차재배면적은 5배, 차생산량은 8배가 증가되었다. 네팔에서 수출되는 차는 2002년을 기점으로 급속하게 증가되었으며 차 수출은 큰 흑자를 가져다 주었다. 네팔차는 1993년 자유무역형태로 수출되었으며 2000년까지 국가가 정책적으로 뒷받침 해 주었다. 외국 차 수입업자와 국내 소규모 차 생산농민들은 네팔차가 가지고 있는 히말리아 향기와 유기농법에 많은 관심을 가지고 있다. 본 총설은 통계자료를 분석하여 네팔차 산업의 전망과 현황을 고찰 하였다. 현재 네팔 국내 경제에 큰 공헌을 하고 있는 차 제품 수출은 2000년대 국가 차 산업정책으로 지지 받고 있다. The current status of production and export of Nepalese tea (Camellia sinensis (L) O. Kuntze) were reviewed. Tea industry of Nepal owes its roots to colonization of India by British East India Company. It has around 145-year old history of production and marketing. After the establishment of two tea estates in the eastern district of Nepal in 1883, it remained unattended from the government for about 80 years. The government support for tea estate expansion was initiated in 1966, which created favorable environment for several large tea estates establishments in the subsequent decades. In 1990s, the government of Nepal adopted policy of encouraging private sector and small scale entrepreneurs by establishing more manufacturing plants, easing credit lending process and introducing the concept of tea farmers' co-operatives. As the implementation of reform took momentum, there was five-fold increase in tea plantation area and eight-fold increase in total tea production from 1994 to 2007. Similarly, tea export from Nepal has been increased dramatically, especially since 2002, thereby surpassing tea import value, with resultant positive balance in tea trade afterwards. This surge in international trade of Nepalese tea has been mainly attributed to liberal reforms policy of 1993 and subsequent national tea policy in 2000. Moreover, specific characteristics such as peculiar Himalayan flavor, organic production methods and comparative advantage for Nepalese tea producers have been foci of attraction of this enterprise to small farmers within the country and foreign investors from abroad. This review article has mainly analyzed status and prospects of Nepalese tea industry in the light of available secondary data, the trends of which seem quite encouraging for national economy, given the national tea policy 2000 be fully implemented.

Protective role of Ei24 loss in skin carcinogenesis by deregulation of PKCα-mediated EGFR transactivation

Sushil Devkota,Han-Woong Lee 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.8

The road less traveled: strategies to enhance the frequency of homology-directed repair (HDR) for increased efficiency of CRISPR/Cas-mediated transgenesis

( Sushil Devkota ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.9

Non-homologous end joining (NHEJ), and to a lesser extent, the error-free pathway known as homology-directed repair (HDR) are cellular mechanisms for recovery from double-strand DNA breaks (DSB) induced by RNA-guided programmable nuclease CRISPR/Cas. Since NHEJ is equivalent to using a duck tape to stick two pieces of metals together, the outcome of this repair mechanism is prone to error. Any out-of-frame mutations or premature stop codons resulting from NHEJ repair mechanism are extremely handy for loss-of-function studies. Substitution of a mutation on the genome with the correct exogenous repair DNA requires coordination via an error-free HDR, for targeted transgenesis. However, several practical limitations exist in harnessing the potential of HDR to replace a faulty mutation for therapeutic purposes in all cell types and more so in somatic cells. In germ cells after the DSB, copying occurs from the homologous chromosome, which increases the chances of incorporation of exogenous DNA with some degree of homology into the genome compared with somatic cells where copying from the identical sister chromatid is always preferred. This review summarizes several strategies that have been implemented to increase the frequency of HDR with a focus on somatic cells. It also highlights the limitations of this technology in gene therapy and suggests specific solutions to circumvent those barriers. [BMB Reports 2018; 51(9): 437-443]

Surgical Management of Pyogenic Discitis of Lumbar Region

Pramod Devkota,Krishnakumar R,Renjith Kumar J 대한척추외과학회 2014 Asian Spine Journal Vol.8 No.2

Study Design: Retrospective review of patients who had pyogenic discitis and were managed surgically. Purpose: To analyze the bacteriology, pathology, management and outcome of pyogenic discitis of the lumbar region treated surgically. Overview of Literature: Surgical management of pyogenic discitis is still an infrequently used modality of treatment. Methods: A total of 42 patients comprised of 33 males and 9 females who had pyogenic discitis with a mean age of 51.61 years (range, 16−75 years) were included in this study. All the cases were confirmed as having pyogenic discitis by pus culture report and histopathological examination. The mean follow-up period was 41.9 months. Results: Debridement and posterior lumbar interbody fusion with autologous iliac bone graft was done in all cases. Thirteen (30.95%) patients had other medical co-morbidities. Five cases had a previous operation of the spine, and three cases had a history of vertebral fracture. Three patients were operated for gynaecological problems, and four cases had a history of urological surgery. L4−5 level was the most frequent site of pyogenic discitis. The most common bacterium isolated was Staphylococcus aureus (S. aureus). Radiologically good fusion was seen in the majority of patients. Conclusions: Pyogenic discitis should be suspected in people having pain and local tenderness in the spinal region with a rise in inflammatory parameters in blood. The most common bacterium was S. aureus, but there were still a greater number of patients infected with other types of bacteria. Therefore, antibiotics therapy should be started only after isolating the bacteria and making the culture sensitivity report.

Finite Element Analysis-Based Damage Metric for Airtightness Performance Evaluation of Concrete Tube Structures

Prakash Devkota,장흥운,홍정욱,박주남 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.4

The super speed tube transport (SSTT) system is considered as a strong candidate for next-generation long-distance transport systems because of its anticipated highly efficient performance. Several studies have been conducted on infrastructure systems using concrete to improve the construction and economic feasibility of SSTTs. However, most of these only focused on the applicability of concrete in maintaining its airtightness; the effects of concrete cracks on the airtightness performance have not been investigated. Accordingly, this paper proposes a method using the finite element analysis (FEA) for evaluating the structural damage associated with the airtightness performance of concrete tube structures. An experimental work was conducted to measure this performance by subjecting a concrete tube structure to four stages of displacements at the midspan. Thereafter, to analytically measure the structural damage, the FEA was conducted and the results were compared with the experimental values. It is found that the structural damage expressed in terms of the eroded volume fraction (VF) calculated at each stage is closely correlated with the corresponding airtightness performance obtained from the experiment. These results show that FEA can be efficiently used for conducting experiments in the SSTT design phase to verify the concrete tube’s airtightness performance under many load cases.

누출률 모델을 이용한 콘크리트 진공 튜브구조의 압력 변화 해석

프러카시데브코타(Devkota, Prakash),박주남(Park, Joonam) 대한토목학회 2017 대한토목학회 학술대회 Vol.2017 No.10

Removal of ethylene from the enviornmrtal air by plasma catalytic oxidation usnig silver and managenes supported zeolite

( Shreedhar Devkota ),목영선,조진오 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0

The emission of various VOCs including Ethylene, which is produced agricultural and industrial activities, into the atmosphere has been increasing massively. Non-thermal plasma (NTP) generated by the dielectric barrier discharge (DBD) in combination with catalysts is a good tool for the removal of VOCs. The reactor used in the experiment contains seven compartments. In this work, decomposition efficiency, specific input energy and ozone concentration are mainly analyzed at different flow rates and different input power by using Manganese dioxide and silver oxide loaded and bare 13 X zeolite as catalysts. Bare zeolite showed higher ethylene decomposition efficiency but Ag2O loaded zeolite, ozone concentration was highly lowered.