Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

地震時 옹벽의 安定性에 대한 確率論的 解析

金泳壽,都德洙,徐寅軾 慶北大學校 1992 論文集 Vol.52 No.-

In this paper the stability of gravity retaining wall is analysed through its probability of failure during earthquakes. Mononobe and Okabe, Seed and Whitman, and Elms and Richards methods are used to determine dynamic earth pressures. And Meyerhof's ultimate bearing capacity theory is employed to find bearing capacity, Three possible modes of failure are examined using AFOSM method. To account for the uncertainty in their numerical values, these factors are taken tobe random variables instead of uniquely valued quantities. On the base of the results obtained in this study, it is concluded that (1) the program that is developed by this study is very useful in assessing the reliability walls under both static and seismic conditions : and (2) the probability of failure of a gravity retaining wall is greatly affected by horizontal accelleration.

수중거리센서 제작과 특성에 관한 연구

김진수,김용혁,이덕출 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

연약지반 성토시 사면의 안정에 미치는 Geotextile의 효과

김수일,조삼덕,우제윤 연세대학교 산업기술연구소 1982 논문집 Vol.14 No.2

Geotextiles have been extensively used in road constructions as effective and economical materials performing several functions such as separation, filtration, drainage, protection against frost break, and as reinforcement to improve the load bearing capacity and stability of road. In this study, a method for the analysis of embankment reinforced with geotextile is studied. Computer program based on the simplified Bishop method is modified in order to investigate quantitively the effect of gotextile on the slope stability of embankment on weak soils. Evaluation of the tensile strength required for the design safety factor is also possible utilizing the computer program in this study.

퍼지 데이터를 이용한 불량률(Ρ) 관리도의 설계

김계완,서현수,윤덕균 한국품질경영학회 2004 품질경영학회지 Vol.32 No.2

Using the p chart is not adequate in case that there are lots of data and it is difficult to divide into products conforming or nonconforming because of obscurity of binary classification. So we need to design a new control chart which represents obscure situation efficiently. This study deals with the method to performing arithmetic operation representing fuzzy data into fuzzy set by applying fuzzy set theory and designs a new control chart taking account of a concept of classification on the term set and membership function associated with term set.

비동기식 IMT-2000 시스템의 셀 탐색

김덕수,김강온,김철성 전남대학교 전자통신기술연구소 2001 전자통신기술논문지 Vol.4 No.1

In this work, cell search which is one of the important functions of W-CDMA system in Rayleigh fading channel is studied by cell search simulation of asynchronous IMT-2000 system(3GPP). For the optimization of this process, three stages are considered: 1) slot synchronization, 2) frame synchronization, and 3) scrambling code identification. It is found that key system parameters such as Primary Synchronization Channel (P-SCH). Secondary Synchronization Channel(S-SCH), and Common Pilot Channel (CPCH) loading factor are optimized. It is noted that the smaller optimal threshold value, the larger SNR of the received signal. Therefore, it is important that the optimal threshold value should be selected in the region of SNR

가정용 음식물처리기 디자인에 관한 연구

鄭炅秀,金德南 삼척대학교 1997 論文集 Vol.30 No.3

One of the most critical environmental problem confronting our nation today is the garbage disposals created by foods. Since the foods garbage contain high rate of moisture, creation of offensive odor and filthy water in garbage fill is now zooming up as the major social problems. Various counter measures have been taken by the government agencies concerned to solve the poignant situation. However, steps taken proved to be rather limited to for the larger restaurants or mass messing facilities while the foods garbage disposal for the individual residential house has been rather ignored. Therefore, this treatise, set aims on the study and analysis described below for the development of rational design and the selection of proposed foods garbage disposer which may be directly available and be operated at individual residential house so that house garbage problems be independently solved. Chapter 1 presents the theory that the decrease of volume of the garbage and transforming into the compost are the best option which is concluded following the analysis of economical aspects over the effects of creation of living garbage and consequent disposal process which effects the environments. Chapter 2 analysed the type of foods garbage disposers and the technical trends of prevailing disposal products in the markets in order to select the type which may be applied to urban residential houses. The needs of such disposers are confirmed through the questionaries distributed to the various housewives directly and collecting answers. Chapter 3 proposes realistic conclusion as to the design of practical, compact sized and soft imaged based on the analysis of trends of house appliances and kitchen interior where proposed disposer should be located. Conjoint analysis is applied for the selection of products concept to maximize the efficiency by the user. As the result, the disposer with price range of 250,000 Korean Won with exchangeable cartridge and the daily disposal capacity of 1 kilogram is selected as the most practically applicable one.

수온 변동 자극이 양식 넙치, Paralichthys olivaceus의 비특이적 생체 방어에 미치는 영향

이덕찬,김도형,김수미,강명석,홍미주,김현정,박수일 한국어병학회 2002 한국어병학회지 Vol.15 No.2

본 연구는 넙치에 대한 수온 변동 자극의 영향을 생체 방어적인 측면에서 검토하고자 하였다. 수온 변동 자극은 매일 18℃에서 25℃까지 상승과 하강을 반복하는 방법을 사용하였으며 그 기간은 30일간 지속하였다. 실험어는 온도변화를 주기 시작한 후 28일째까지 무작위로 채포하여 말초혈액 중의 백혈구 수, 전혈의 식작용능, 활성산소, 환원능, chemiluminescence(CL) response 및 lysozyme용균능과 같은 각종 비특이적 생체방어와 관련한 실험을 수행하였다. 수온 변동 자극을 주기 시작한 후 1일째부터 시작하여 1주일 이내에 약 18%의 넙치가 폐사하였으며 이후에는 대조구와 유사하였다. 또한, 수온 변동 자극을 준 실험구의 호중구 수는 2주째에 유의적인 증가를 보였으며, 림프구 수는 2일과 3일째에 감소하였으나 1주째부터 대조구와 유사한 수준으로 회복되었다. 실험구에서는 식작용 결과 식균율과 식균지수에서 이물질 투여 후의 반응이 늦게 나타났다. 식세포의 활성을 조사한 NBT reduction 실험에서는 실험 기간 동안 대조구와 유사한 경향ㅇ르 나타내었다. 또한, CL response의 경우, 실험구는 온도 변동 자극초기에 대조구에 비하여 유의적으로 낮았다. 그리고, 식세포가 분비하는 용균성 효소인 점액 내 lysozyme의 활성은 실험 기간 동안 실험구에서 낮게 나타나는 경향을 보였다. 이러한 여러 가지 비특이적 생체 방어 반응의 저하 현상은 수온 변동 자극 초기에 나타났으며, 1주일 정도 지속하면 그 환경에 적응하는 것을 알 수 있었다. 그리고 이러한 현상은 이들이 초기 폐사 발생과 어떤 상관 관계에 있는 것으로 생각되었다. This study was performed to know the effects of stress nduced by the daily fluctuation of water temperature fro 18? to 25? up and down for 30 days on the defence mechanism of olive flounde,r Paralichthys olivaceus. To make clear the temperature stress on the defense mechanism of the tested fish, several factors of immune response such as counting of leucocyte appearance in periopheral blood, phagocytic activity in whole blood cells, nitroblue tetrazolium(NBT) reduction, chemiluminescence(CL) response, and lysozyme activity were investigated at 28 days after giving the change of water temperature. The fish was controlled under the none feeding condition during experimental period. Mortality of the tested fish was rapidly increased up to 22% within the first one week of the experimental period without any additional stress factors. The number of neutrophil of peripheral blood in the tested group was significantly higher than the control group at the 2nd week, but the number of lymphocyte was significantly lower than the control group at the 1st and 3rd day of the experimental period, respectively. In the NBT reduction test, the activity of macrophage in the control group fish was the highest on the 7th day while that in the tested group was on the 3rd day. Also, the phagocytosis of tested group against formalin killed cells was retarded compared with the control CL response of the tested group was significantly lower from 2nd to 5th day of the experimental period than the contro. The lysozyme activity of tested group was remained higher during the experimental period than the contro. Even through the tested fish showed different results in some non-specific factors of immune responses between tested and control group fish, olive flounder seems highly adaptable in repeated water temperature change in condition after one week under the given temperature fluctuation range

어류에 발생하는 Mycobacterium 속 세균성 질병에 대하여

이덕찬,김도형,박수일 한국어병학회 2001 한국어병학회지 Vol.14 No.3

Mycobacterium 속 균은 일반적으로 Gram 양성 및 음성균의 세포벽에서 관찰되는 구조 및 성분과 차이가 나며, 장시간 동안 배양해야 하는 등의 특성이 있다. Mycobacterium속 세균의 세포벽에는 다량의 mycolic acid가 함유되어 일반 염색법으로느 염색이 잘되지 않으나 일단 염색되면 산이나 알칼리에 탈색되지 않는 성질을 가지고 있으므로 항산성균이다. 그람 양성 간균, 비운동성, 호기성이며 아포는 없고 발육이 느리다. 그러므로 이러한 균의 배양, 분리 그리고 동정에는 Gram 양성 및 음성균에서 수행하는 일반적인 생화학 및 생리학적 방법 외의 다양한 시험들이 필요하다. Atexander(1913)에 의하여 'piscine tuberculosis'로 보고된 후 어류에 피해를 주는 여러 종류의 Mycobacterium 속의 세균군이 보고되었다. 최근까지 해수어와 담수어 40과 151종 이상의 어류에서 감영 가능성이 보고되어 있으며 특히, 열대성 담수어와 해수어, 온대성 담수어류 및 관상어류 등에서 주로 보고되었다. 어류에 감염을 일으키는 균으로는 M. marinum, M. chelonae 및 M. fortuitum이 잘 알려져 있으나, 최근 어류뿐만 아니라 양서류, 파충류 및 갑각류 등에서 M. simiae, M. scrofulaceum, M. poriferae, M. kansasii 및 M. peregrinum 등과 미국의 Chesapeake 만의 자연산 sea bass에서 Mycobacterium chesapeake sp. nov.이 보고되었다. 어류의 mycobacteriosis는 오염된 먹이나 수중 부유물질에 부착된 병원체의 어체 상처 접촉에 의하여 수평적으로 확산될 수 있다. 감염된 어류는 먹이 섭취 불량, 운동성 저하, 복수 저류로 인한 복부팽만 등의 외부증상과 신장, 비장 및 간 등의 육아종 형성 등 전신적, 만성적 그리고 진행성 병변을 보인다. 어류에 질병을 일으키는 Mycobacterium sp는 65kDa과 <14kDa의 분자량을 가진 ECP를 생산하며, 세균 종에 따라 mucinase, lipase 또는 RNase를 생성한다. M. marinum에 의하여 생성된 ECP에 의한 독작용은 Vibrio anguillarum이나 Aeromonas hydrophila ECP에 비하여 약하며, 무지개송어와 나일틸라피아에 대한 LD_50은 400μg fish^-이다. 이들 병원균에 의한 감염증 발생 후 항생제를 사용하면 어류의 폐사는 줄어들지만 완전한 치료는 어려우므로 예방 조치가 필요할 것이다. 그리고 어류에 감염을 일으키는 Mycobacterium sp.에 의한 인체감염이 전세계적으로 보고되 있으며, 국내에서도 환경이나 사람의 객담(喀痰, sputum)으로부터 M. marinum과 M. fortuitum complex가 분리 보고되고 있으므로 앞으로 어류로부터의 감염에 대한 많은 연구가 필요하다. Bacteria of the genus Mycobacterium ard generally rod-shape, gram-positive, nonspore-forming, non-motile and acie-fast. They are quite different from the structures and compounds in common bacteria. The bacteria are not decolorized with acid-alcohol because of the cell walls composed of mush mycolic acis. The bacteria are relatively fastidious, growing very slowly on only a few special media. Therefore many kinds of experimental methods should be needed to isolate and identify mycobacteris besides physiological and biochemical methods commonly used. Serveral bacteria of the genue Mycobacterium have been reported after Alexander(1913)described piscine tuberculosis to refer to fish mycobacteriosis. Probablility of infection from 40 families, 151 species of fich to human hsd been reported until recent year, particularly including tropical freshwater and marine fishes, temperate frashwater fishes and aquarium fishes. The causative agents of the fish mycobacteriosis involve M. marinum, M. chelonae and M. fortuitum. Currently M. simiae. M. scrofulaceum, M. poriferae, M. kansasii and M. peregrinum have aldo been found in amphibians, reptiles and crustaceans and M. cheapeaki sp. nov. was isolated from wild sea bass in the gulf of Chesapeake, the US. The most probable route of transmission for fish mycobacteriosis is oral or through injuries of the skin which can be exacerbated by external parasites. Mycobacteriosis is a chronic progressive disease, with various clinical signs, including lack of movement coordination, abdominal swelling, inappetence, emaciation and inflammation of the skin. Interally greyish white nodules develop on various organs, particularly the liver, kidney and spleen. ECP from virulent Mycobacterium sp. has two different proteins, a 65 kDa and <14 kDa, and the ECP may contain mucinase, lipase or RNase depending on the kinds of bacteria. The ECP of M. marinum, with the LD_50 dose equated to 400㎍ fish^-1 for rainbow trout and Nile tilapia, is more toxie than those of Vibrio anguillarum and Aeromonas hydrophila. Prevention of the disease is perhaps the single most economical means to combat mycobacteriosis, since infected fish cannot be completely cured althouth the mortality may be reduced by application of antibiotics. It may be difficult to ruld out the possibility of human infection by Mycobacterium sp. which is virulent to fish, as the related reports have been described worldwide and also reported domestically on isolation of M. marinuma and M. fortuitum complex from the environment and sputum.

다중 채널 동축 케이블의 초고주파 측정용 보드 설계

문수덕,김진규,황희용 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

In High-Speed DSP systems, crosstalk between transmission lines of multi-channel can degrade the performance of equipment operations, This paper presents a microwave board to measure multi-channel coaxial cable assembly. The designed board has good performances from DC to 3 GHz, which have improved characteristic impedance, reduced crosstalk by using via fence, and low transmission loss. Using the designed board, we can measure characteristics of DUT(Device Under Test) such as return loss, insertion loss, crosstalk, phase delay, and characteristic impedance. The measured results are used to improve performances of a produced coaxial cable assembly.