Azathioprine에 반응을 보인 류마티양 관절염에 의한 경막염(Pachymeningitis)1예

배광봉,이정호,이정찬,곽상혁,강정현,김철희,김현수,김종숙,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Rheumatoid arthritis frequently involves the cervical spine and may lead to neurologic impairement. However, direct involvement of CNS structures by inflammatory cells has been reported infrequently. The prevalence of this complication of rheumatoid arthritis is unknown. Inflammatory CNS involvement in rheumatoid arthritis reportedly occurs in the setting of longstanding, active, erosive articular disease and is accompanied by extracranial and extraspinal nodules and vasculitis. This is diagnosed by radiologic finding of CNS nodules or meningeal thickening and by biopsy or autopsy. Treatment with corticosteroid, cytotoxic agent or surgical decompression is helpful. But the majority of patients die within several months of onset of neurologic symptom. Recently, we experienced a case of pachymeningitis caused by rheumatoid arthritis, which resolved repeatedly with azathioprine treatment.

항암화학요법을 받는 암 환자에 대한 Nucare^R의 영양지지효과

조덕연,김현수,곽상혁,강정현,김철희,배광봉,김종숙,박상준,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Purpose : The purpose of this study was to evaluate the effect of nutritional support with enteral liquid supplement in cancer patients receving chemotherapy for possible benefit in nutritional, immunologic and golbal function of patients. Patients and Methods : From October 1995 to February 1997, 30 advanced cancer patients receving chemotherapy were divided two roups. The Nycare group, in addition to normal diet, Nucare^R enteral supplement was given for 1week right after chemotherapy for the duration of 2 chemotherapy cycles. Control group received only normal diet without parenteral fluid supplement for 2 chemotherapy cycles. Results : Median ages were 53 end 56 years for Nucare group and Contrl group respectively. Performance scores was less than 2 by ECOG scale. All patients were stage Ⅲ or Ⅳ. The physical parameters, such as weight, arm muscle circumference(AMC) and triceps skin fold(TSF) were decreased in both groups after 2 cycles of anticancer therapy. but it was less severe in Nucare group(p<0.05). Serum transferrin was maintained in mild deficit state in Nucare group, whereas it aggravated form mild to moderate deficit in Control group(p<0.05). Serum albumin level increased in Nucare group without statistical significance. but it decreased from normal to mild deficit in Control group. Serum total protein did not change significantly in Nucare group. but in Control group, serum total protein was decreased from 7.24±0.9 to 6.52±0.5(P<0.05). Total lymphocyte count did not change significantly in both groups. Conclusion : This study shows that the nutritional support with Nucare^R was effective in the prevention of nutritional deficit status in patients receving a nticancer chemotherapy.

半夏의 修治方法에 따른 細胞毒性硏究

李秀培,金德宗,金康柱,田炳勳,禹元洪 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

The purpose of this study is to know the effects of Pinelliae Tuber on the cytotoxicity of human gingival fibroblasts according to brewing methods. Pinelliae Tuber was extracted by water in the state of being raw materials, prebrewing with Zingiberis Tuber and alumen. The extracted Pinelliae Tuber was applied to the cultured human gingival fibroblasts. The cytotoxic effct of Pinelliae Tuber was meaeured by MTTassay and microscopic findings. The results were as follows : 1. The cell viability of the fibroblasts by Pinelliae Tuber water extracts significantly decreased compared to control group. 2. The cell viability by the Pinelliae Tuber extracts prebrewed with Zingiberis Tuber was similiar to those of control group. 3. The cell viability by the Pinelliae Tuber extracts prebrewed with alumen slightly decreased, but was higher than those of PT group. 4. According to the microscopec findings, the number of cell count were smaller than normal in all experimental group, specially in the PT group, and showed the shrinkage of cytoplasmic process in the PT group and PA group.

Imide 단위를 포함하는 Polyamide의 합성과 열적 성질

길덕수,배장순,공명선 단국대학교 신소재기술연구소 1997 신소재 Vol.7 No.-

Imide 단위를 포함하는 diamine과 dicarboxylic acid 단량체들, N-(4-aminophenyl)-4-aminophthalimide(1), N-(4-carboxyphenyl)-4-carboxyphthalimide(2), N,N'-oxydiphenylenebis(4-aminophthalimide)(3) 및 N,N'-oxydiphenylenebis(4-carboxyphthalimide)(4)를 합성하였다. 위의 단량체들은 서로 축합하여 imide 단위가 교대된 poly(imide-amide)s를 합성하였으며 imide 단위를 포함하는 diamine 단량체들은 terephthaloyl chloride, isphthaloyl chloride와 축합하여 또다른 poly(imideamide)s를 합성하였다. 이렇게 합성된 중합체들은 NMP/LiCl 용액에 매우 잘 용해하였으나 그밖에 극성 반양자성 용매인 DMF, DMSO 및 DMAc 등에는 낮은 용해도를 보여주었다. 고유 점성도는 0.15-0.51 dL/g 이었으며 NMP/LiCl 용액으로부터 단단하지만 취성이 있는 고체막이 형성되었다. 중합체들의 유리전이온도 및 융점은 관찰되지 않았으나 400℃ 부근에서 서서히 분해가 시작되었으며, 중합체들은 500℃에서 82-93%의 잔사량을 나타내어 우수한 열적성질을 보여주었다. Diamine and dicarboxylic monomers with imide linkage, N-(4-aminophenyl)-4-aminophthalimide(1), N-(4-carboxyphenyl)-4-carboxyphthalimide (2), N,N'-oxydiphenylenebis (4-aminophthalimide)(3) and N,N'-oxydiphenylenebis (4-carboxyphthalimide) (4) were prepared, and poly(amide-imide)s with ease processing characteristics were prepared from the monomers, 1-4 and terephthaloyl chloride or isophthaloyl chloride. Intrinsic viscosity of polymers was 0.15-0.51 dL/g and brittle hare films were obtained by casting the NMP/LiCl polymer solution on to glass plate. The poly (amide-imide)s are easily solubiel in NMP/LiCl and also in polar aprotic solvents such as DMF, DMSO, NMP and DMAc at 80℃. These polymers showed no glass transition temperature and melting temperature in their DSC thermograms. All the polymers decomposed at around 400℃ and 500℃ in nitrogen and had 88∼93% residual weight at 500℃.

치수 치근단 병소의 전구 위험요인으로서의 제 2 형 당뇨의 역할에 관한 소고

김진희,배광식,서덕규,홍성태,이윤,홍삼표,금기연 大韓齒科保存學會 2009 Restorative Dentistry & Endodontics Vol.34 No.3

Diabetes Mellitus (DM) is a syndrome accompanied with the abnormal secretion or function of insulin, a hormone that plays a vital role in controlling the blood glucose level (BGL). Type 1 and 2 DM are most common form and the prevalence of the latter is recently increasing. The aim of this article was to assess whet her Type 2 DM could act as a predisposing risk factor on the pulpo-periapical pathogenesis. Previous literature on the pathologic changes of blood vessels in DM was thoroughly reviewed. Furthermore, a histopathologic analysis of artificially-induced periapical specimens obtained from Type 2 diabetic and DM-resistant rats was compared. Histopathologic results demonstrate that the size of periapical bone destruction was larger and the degree of pulpal inflammation was more severe in diabetic rats, indicating that Type 2 DM itself can be a predisposing risk factor that makes the host more susceptible to pulpal infection. The possible reasons may be that in diabetic state the lumen of pulpal blood vessels are thickened by atheromatous deposits, and microcirculation is hindered. The function of polymorphonuclear leukocyte is also impaired and the migration of immune cells is blocked. leading to increased chance of pulpal infection. Also, lack of collateral circulation of pulpal blood vessels makes the pulp more susceptible to infection. These decrease the regeneration capacity of pulpal cells or tissues, delaying the healing process. Therefore, when restorative treatment is needed in Type 2 DM patients, dentists should minimize irritation to the pulpal tissue under control of BGL. 당뇨(Diabetes Mellitus)란 혈당을 조절하는 인슐린의 분비나 기능에 장애를 야기하는 질환으로 인슐린 의존성 여부에 따라 제 1 형과 제 2 형으로 분류된다. 본 종설은 최근 증가 추세에 있는 제 2 형 당뇨가 치수 치근단 병소의 병인 과정에 전구 위험 요인으로 작용할 수 있는지를 평가 하고자 문헌고찰을 통해 당뇨의 병인 과정에서 특징적으로 나타나는 혈관 합병증에 관해 알아보고, 부가적으로 제 2 형 당뇨 쥐 모델에서 인위적인 치수감염 후 얻은 치근단 조직의 조직병리학적 분석을 시행하였다. 조직학적 관찰 결과 제 2 형 당뇨 쥐에서 대조군에 비해 치수 치근단 병소의 크기가 증가하였고, 치수 염증 반응도 심하게 나타난 것으로 보아 당뇨 자체가 숙주를 감염에 취약한 상태로 만드는 전구 위험요소로 작용하였음을 알 수 있었다. 이러한 이유로는 첫째, 당뇨 시 전반적으로 나타나는 혈관 내 죽상 침착(atheromatous deposits)에 의해 혈관 내벽의 두께가 두꺼워져 미세 순환의 장애는 물론 탐식 세포의 기능 저하, 면역 세포의 혈류 이동이 차단되어 치수 감염 시 쉽게 치근단 병소로 이환될 가능성이 높고, 둘째 치수 혈관에서 특징인 측부 순환(collateral circulation)의 부재에 따른 살균성 다형핵 백혈구의 활동 억제를 포함한 미세 혈관계의 취약성으로 인해 치수 조직의 재생능이 저하되어 추가적인 감염원의 공격에 대한 방어 및 치유 저하를 더욱 심화시키기 때문인 것으로 사료된다. 따라서 제 2 형 당뇨 환자의 수복치료 시 치과의사는 당뇨 조절 하에서 치수 조직의 자극을 최소화하기 위한 세심한 처치가 필요하다.

익산지역 강수성분의 연차적 변이

이경보,이덕배,이상복,김재덕,박찬원 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

野山開墾地에서 大豆栽培 硏究 : 石灰 및 窒素 供給 形態가 養分吸收 및 收量에 미치는 영향 1. Influence of Application of Lime and Kinds of Nitrogen Fertilizer on Nutrients Content and Yield

權泰午,李德培 圓光大學校 1994 論文集 Vol.28 No.2

野山 開墾地에서 대두 재배시 석회(중화량시용, 무시용) 및 질소공급 형태(무시용, 요소, 유안, 초안)에 따른 대두 生育 및 植物體 中 窒素化合物 조성과 收量등에 대하여 검토한 결과, 1.生育 및 養分吸收에 있어 석회 시용은 무시용에 비하여 식물체의 乾物重및 葉, 莖의 T-N, 水可溶性 窒素化合物, 葉의 無機成分含量등은 증가되었으나 葉의 炭水化物 함량은 감소되었으며 석회 시용구에서 窒素肥種間에는 초안구가 요소, 유안구보다도 乾物重, T-N, Amino acid-N, 무기성분함량은 증가되었으나 Allantoin-N 및 탄수화물 함량은 減少되는 경향이었다. 2.種實 中 養分含量에 있어 석회 시용구는 무시용에 비해 炭水化物, 租蛋白, CaO함량 등은 증가되었으나 K₂O함량은 감소되었고, 질소비종간에는 탄수화물, 租脂肪은 질소무시용구에서 높았으며 조단백은 유안구, CaO는 유안구 및 초안구, P₂O5는 요소구에서 대체로 높았다. 3.수량 및 수량 구성요소에 있어 석회 시용으로 무시용에 비해 百粒重 및 總莢數에서 증가되어 20%의 增收를 가져왔으며 석회 시용구에서 질소비종간에는 초안구에서 總莢數가 증가되어 질소 무시용보다 13% 증수되어 수량이 제일 높았고, 유안, 요소, 질소, 무시용순이었다. 4.葉의 T-N, Amino acid-N함량과 수량과는 正의 有意的인 相關을 나타내었으나 Allantoin-N함량과는 負의 相關을 보였고, 莖에 있어서는 生育後期 Amino acid-N함량과 수량과는 有意性이 없고, Allantoin-N함량과는 生育初期에는 負의 상관이었으나 生育後期에는 유의적인 正의 상관을 보였다. This study was carried out to investigate influence of application of lime and kinds of N fertilizer on nutrients content and yield of soybean in newly reclaimed area. Lime was applied requiring amount for neutralization and nitrogen was applied 4kg per 10a. Kinds of N fertilizer were urea, ammonium sulfate, and ammonium nitrate. Lime application caused to neutralize soil pH, and resulted in more nutrient uptake in the soybean plant than of no application of lime. As result of that, dry matter production was increased, and weight of 100 seeds and number of total pods were also increased. These factors caused to increase yield of soybean. T-sugar and starch, and crude protein etc. in seeds were increased with lime application. Ammonium nitrate under lime application resulted in more increase of dry matter production, content of inorganic elements, number of total pods, and yield than those of urea and ammonium sulfate. Dominating N form in vegetative growth stage was free amino acid-N transformed from chemical N fertilizer, but in reproductive growth stage was allantoin-N. Significant positive relationship were found between content of T-N and amino acid-N in the leaf, and yield, but negative relationship was found between content of allantoin-N and yield. Content of allantoin-N in the stem of late reproductive growth stage was significantly related to yield, but no significantly relationship was found between content of amino acid-N and yield.

만경강 본류의 자연정화능 향상을 위한 식생학적 진단

이경보,김창환,김종구,이덕배,박찬원,나승용 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

만경강 본류를 대상으로 수질을 개선하기 위한 일환으로 하천식생의 종구성, 하천형태에 따른 군락의 구조, 단면분석을 통한 현존식생의 분포 등을 종합적으로 분석하여 하천 생태계에 대한 종합적 해석과 식생에 의한 자연정화능 활용방안을 평가하고자 2001년 6월부터 2002년 3월까지 수질 및 식생에 관한 자연정화능을 조사, 평가하였다. 만경강 본류 수질중 T-N의 농도는 갈수기인 겨울철이 17.23 mg/L로 가장 높았으며, 여름철이 8.59 mg/L로 낮았고, 상류보다는 하류에서 그 농도가 높았다. 또한 평균 T-P의 농도는 여름철과 가을철에 각각 0.45, 0.47 mg/L로 비슷하였으나 겨울철에 1.79 mg/L로 농도가 높아졌다. BOD의 농도는 상류유역에서 0.95~2.57 mg/L로 Ⅰ~Ⅱ급수 수질을 나타내었으나 하류유역에서는 6.87~9.72 mg/L로 Ⅴ급수에 가까운 수질이었다. 만경강 상류의 식물상은 73과 134속 218종 33변종으로 총 251종류가 조사되었으며, 중류 일대에서 조사된 식물은 33과 69속 82종 16변종으로 총 98종류가 조사되었다. 그리고 만경강 하류 일대에서 관찰된 식물은 27과 58속 71종 14변종으로 총 85종류가 조사되었다. 만경강 유역에서 자연정화능에 의한 수질개선을 도모하기 위해 식물 생활형별로 하천 정화능이 뛰어난 식물을 조사 분석한 결과 침수식물은 붕어마름, 검정말, 부엽식물은 어리연꽃, 왜개연꽃, 추수식물은 갈대, 줄, 부들, 습생식물은 큰황새 냉이, 고마리 등이 질소, 인 흡수능이 우수한 식물이었다. This study was conducted to get some information on plants abilities to enhance water purification and to find out away to conserve the ecosystem in Mankyeong river. Vegetation were surveyed at 4 sites pointing by 1:5,000 topographical map, from June 2001 through March 2002. T-N content in water were high in all sites of Mankyeong river, the average T-N levels were 8.59 and 17.23 ㎎/L summer and winter, respectively. The average T-P level during summer was 0.47 ㎎/L, but that was 1.79 ㎎/L during winter. The BOD level in Mankyeong upstream ranged from 0.95 to 2.57 ㎎/L which would be in Ⅰ or Ⅱ grade according to water quality criteria by Ministry of Environment, but BOD level in Mankyeong downstream ranged from 6.87 to 9.72 ㎎/L. The plant species of river flora were found 251, 98 and 85, upstream, midstream and down stream, respectively. Among the surveyed plants, Ceratophyllum demersum, submerged plant and Nuphar subinteperrimum took up higher contents of phosphate and nitrogen than other plants. The Phragmites communis and Zizania latifolia having much biomass were thought to be suitable plants for enhancement of the natural water purification.