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Soonja Oh,William W. Adams III,Barbara Demmig-Adams,Seok Chan Koh 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1
Korean fi r is an endemic ornamental tree species facing population decline in Korea. To further understand the acclimatoryadjustments it undergoes in response to seasonal extremes, we characterized some of the needle proteins that are upregulatedduring winter. Two-dimensional gel electrophoresis (2-DE), followed by MALDI TOF/TOF MS/MS and Mascot analyses,was used to visualize changes in protein profi les during acclimation to winter stress. From the 2-DE protein profi les ofKorean fi r needles, 226 protein spots were detected, many of which accumulated at higher levels during the winter. Among17 proteins identifi ed, 12 matched proteins associated with photosynthesis and with biotic and abiotic stresses, and eightwere signifi cantly upregulated under winter stress. Upregulated proteins included photosynthetic enzymes sedoheptulose-1,7-bisphosphatase and fructose bisphosphate aldolase of the Calvin–Benson cycle, four proteins related to oxidative stresstolerance, two proteins implicated in biotic defense, one heat-shock protein, and fi ve unknown proteins. However, two otheroxidative-stress-related proteins were present at high levels throughout the year, and a chitinase and the small subunit ofribulose-1,5-bisphosphate carboxylase showed no seasonal adjustments. Thus, Korean fi r needles exhibited winter upregulationof some photosynthetic enzymes, coupled with increased photo protective thermal energy dissipation, and proteins relatedto abiotic and biotic stress resistance. Winter stress, which can include both low temperature and reduced water availability,in the subalpine region of Mount Halla led to an altered physiological equilibrium with increases in key Calvin–Bensoncycle enzymes and increased enzymatic and non-enzymatic protection against oxidative stress.