Activation of 4-1BB signaling in bone marrow stromal cells triggers bone loss via the p-38 MAPK-DKK1 axis in aged mice

Wan Daqian,Ai Songtao,Ouyang Huoniu,Cheng Liming 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Senile osteoporosis can cause bone fragility and increased fracture risks and has been one of the most prevalent and severe diseases affecting the elderly population. Bone formation depends on the proper osteogenic differentiation of bone marrow stromal cells (BMSCs) in the bone marrow microenvironment, which is generated by the functional relationship among different cell types in the bone marrow. With aging, bone marrow provides signals that repress osteogenesis. Finding the signals that oppose BMSC osteogenic differentiation from the bone marrow microenvironment and identifying the abnormal changes in BMSCs with aging are key to elucidating the mechanisms of senile osteoporosis. In a pilot experiment, we found that 4-1BBL and 4-1BB were more abundant in bone marrow from aged (18-month-old) mice than young (6-month-old) mice. Meanwhile, significant bone loss was observed in aged mice compared with young mice. However, very little data have been generated regarding whether high-level 4-1BB/4-1BBL in bone marrow was associated with bone loss in aged mice. In the current study, we found upregulation of 4-1BB in the BMSCs of aged mice, which resulted in the attenuation of the osteogenic differentiation potential of BMSCs from aged mice via the p38 MAPK-Dkk1 pathway. More importantly, bone loss of aged mice could be rescued through the blockade of 4-1BB signaling in vivo. Our study will benefit not only our understanding of the pathogenesis of age-related trabecular bone loss but also the search for new targets to treat senile osteoporosis.

Simultaneous removal of SO2 and NOX with ammonia absorbent in a packed column

Yong Jia,Daqian Du,Xinxi Zhang,Xilou Ding,Oin Zhong 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.9

Catalytic oxidation of NO followed by simultaneous removal of SO2 and NOX with ammonia is a promising method for control of coal-fired flue gas pollutants. We investigated simultaneous absorption of SO2 and NOX in a packed column with ammonia, and found that SO2 and NOX could promote absorption with each other in the process of simultaneous removal SO2 and NOX. The removal efficiency of SO2 and NOX was, respectively, about 98% and 70.9% at pH 5.5, temperature 323.15 K, SO2 concentration 1,800×10−6, NOX concentration 400×10−6 and mNO2/mNO 1 in our experimental system. The experimental results also show that the formation of sulfite oxidized by reacting with dissolved NO2and the molar ratio of sulfite to total sulfur is more than 0.8 in the solution. Accordingly, the energy consumption for sulfite oxidation would be greatly reduced in the process of simultaneous desulfurization and denitrification with ammonia.

Experimental Study on Tire-Road Dynamic Contact Pressure Distribution Using FTIR Imaging

Liang Chen,Zhu Daqian,Wang Guolin,Shan Maoqing 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.5

The power source for accelerating, braking, and steering of the vehicle is derived from the contact force between tire and road. Therefore, the pressure distribution in the tire-road contact patch is imperative for explorative studies into tire and vehicle performance. A test bench for measuring tire-road dynamic contact pressure distribution was designed based on the frustration of total internal reflection (FTIR) imaging. The load, camber angle, slip angle, and rolling speed of the tire can be adjusted by the bench. The factors, including the color of calibration material and the ambient light, were studied to improve the calibration accuracy of the bench and solve the problem of recurring calibration of the bench at regular intervals. From the image captured by the camera, the contact region between the tire and the road was obtained by image processing technologies, including image correction, graying, filtering, and segmentation. The obtained contact region was converted into contact pressure distribution by the parameters obtained from calibration subsequently. Finally, the dynamic contact pressure distribution under different conditions, including different inflation pressure, load, camber and slip angles, was analyzed and the error is within a reasonable range.

Research on the optimization method for PGNAA system design based on Signal-to-Noise Ratio evaluation

JiaTong Li,WenBao Jia,DaQian Hei,Zeen Yao,Can Cheng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.6

In this research, for improving the measurement performance of Prompt Gamma-ray Neutron ActivationAnalysis (PGNAA) set-up, a new optimization method for set-up design was proposed and investigated. At first, the calculation method for Signal-to-Noise Ratio (SNR) was proposed. Since the SNR could becalculated and quantified accurately, the SNR was chosen as the evaluation parameter in the newoptimization method. For discussing the feasibility of the SNR optimization method, two kinds of PGNAAset-ups were designed in the MCNP code, based on the SNR optimization method and the previous signaloptimization method, respectively. Meanwhile, the single element spectra analysis method was proposed, and the analysis effect of single element spectra as well as element sensitivity were used forcomparing the measurement performance. Since the simulation results showed the better measurementperformance of set-up designed by SNR optimization method, the experimental set-ups were built forthe further testing, finally demonstrating the feasibility of the SNR optimization method for PGNAA setup design

Isolation and Proteomic Analysis of Extracellular Vesicles from Lactobacillus salivarius SNK-6

Huang Jiwen,Zhao Ayong,He Daqian,Wu Xiao,Yan Huaxiang,Zhu Lihui 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1

The proteins carried by the extracellular vesicles of Lactobacillus salivarius SNK-6 (LsEVs) were identified to provide a foundation for further explorations of the probiotic activities of L. salivarius SNK-6. LsEVs were isolated from the culture media of L. salivarius SNK-6 and morphological analysis was conducted by scanning electron microscopy. Subsequent transmission electron microscopy and nanoparticle tracking analysis were performed to assess the morphology and particle size of the LsEVs. In addition, the protein composition of LsEVs was analyzed using silver staining and protein mass spectrometry. Finally, internalization of the identified LsEVs was confirmed using a confocal microscope, and enzyme-linked immunosorbent assay was employed to analyze the levels of inflammatory cytokines in LPS-challenged RAW264.7 cells. The results revealed that the membraneenclosed LsEVs were spherical, with diameters ranging from 100–250 nm. The LsEVs with diameters of 111–256 nm contained the greatest amount of cargo. In total, 320 proteins (10–38 kD) were identified in the LsEVs and included anti-inflammatory molecules, such as PrtP proteinase, cochaperones, and elongation factor Tu, as well as some proteins involved in glycolysis/gluconeogenesis, such as fructose-1,6-bisphosphate aldolase. Enrichment analysis showed these proteins to be related to the terms “metabolic pathway,” “ribosome,” “glycolysis/gluconeogenesis,” “carbohydrate metabolism,” and “amino acid metabolism.” Furthermore, the LsEVs were internalized by host liver cells and can regulate inflammation. These findings confirm that LsEVs contain various functional proteins that play important roles in energy metabolism, signal transduction, and biosynthesis.

The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect

Liu Wei,Huang Yong,Liu Daqian,Zeng Teng,Wang Jingzhe,Li Ang,Wang Dawei,Wang Xiaoyu 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: In the present study, a novel tissue engineering bone graft including platelet rich plasma gel (PRP gel), human umbilical mesenchymal stem cells (HUMSCs) and nanohydroxyapatite/polyamide 66 (nHA-PA66) was constructed. We explored whether the composite scaffolds could enhance the angiogenesis and bone repair capacity in rat femoral large bone defect (LBD). This study aimed to provide evidence for the clinical application of the composite scaffold in LBD treatment. METHODS: PRP was prepared, the platelets and growth factors were measured. HUMSCs were isolated and identified. the osteogenic capacity of PRP in vitro was measured. Then HUMSCs-PRP-gel/nHA-PA66 composite scaffolds were synthesized and observed. The proliferation and osteogenesis differentiation of HUMSCs on the composite scaffold was measured. The angiogenic capacity of PRP in vitro was measured by capillary-like tube formation assay. Finally, the angiogenesis and bone repair capacity of the composite scaffolds was measured in rat LBD. RESULTS: PRP contained high level of platelets and growth factors after activation, and promoted osteogenic and angiogenic differentiation in vitro. The HUMSCs-PRP-gel/nHA-PA66 composite scaffold was porosity and promoted the proliferation and osteogenesis differentiation of HUMSCs. At 12th weeks, more micro-vessels and new bone were formed around the composite scaffolds compared with other groups, the defect was almost repaired. CONCLUSION: Our study for the first time identified that the combination of PRP gel, HUMSCs and nHA-PA66 scaffold could significantly promote angiogenesis and bone regeneration in rat LBD, which may have implications for its further application in clinical LBD treatment.

Optimization of image reconstruction method for dual-particle time-encode imager through adaptive response correction

Zhao Dong,Jia Wenbao,Hei Daqian,Cheng Can,Cheng Wei,Liang Xuwen,Li Ji 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.5

Time-encoded imagers (TEI) are important class of instruments to search for potential radioactive sources to prevent illicit transportation and trafficking of nuclear materials and other radioactive sources. The energy of the radiation cannot be known in advance due to the type and shielding of source is unknown in practice. However, the response function of the time-encoded imagers is related to the energy of neutrons or gamma-rays. An improved image reconstruction method based on MLEM was proposed to correct for the energy induced response difference. In this method, the count vector versus time was first smoothed. Then, the preset response function was adaptively corrected according to the measured counts. Finally, the smoothed count vector and corrected response were used in MLEM to reconstruct the source distribution. A one-dimensional dual-particle time-encode imager was developed and used to verify the improved method through imaging an AmeBe neutron source. The improvement of this method was demonstrated by the image reconstruction results. For gamma-ray and neutron images, the angular resolution improved by 17.2% and 7.0%; the contrast-to-noise ratio improved by 58.7% and 14.9%; the signal-to-noise ratio improved by 36.3% and 11.7%, respectively.

A design of transmission-type multi-target X-ray tube based on electric field modulation

Zhao, Lei,Jia, Wenbao,Jin, Limin,Shan, Qing,Cheng, Can,Zhu, Hongkui,Hei, Daqian Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.9

Multi-target X-ray tube is a new type X-ray source, and can be applied in many fields such as sensitive X-ray fluorescence analysis and medical imaging. In this work, we report an electric field modulation multi-target X-ray tube, which contains four targets (Cr, Ni, Au, Mo) coated on a Beryllium (Be) window. A four-valve electric field deflector was developed to deflect the electron beam to bombard the corresponding targets. Particle dynamics analysis software was employed to simulate the particle tracking of electron beam. The results show that the 30 keV electron beam could get a 6.7 mm displacement on the target plane by 10⁵ V/m electric field. The focus areas are about 2 mm × 5 mm and 4 mm × 2.5 mm after deflection in two directions. Thermal behavior calculated by ANSYS shows that the designed target assembly could withstand a 10 W continuous power. The optimum target thicknesses and emission spectra were obtained by Geant4 when the thickness of Be window was 300 mm and the electron beam incident angle was 0.141 rad. The results indicate that this multi-target X-ray tube could provide different X-ray sources effectively.

Clinical Effect of Traditional Chinese Medicine Shenhuang Granule in Critically Ill Patients with COVID-19: A Single-Centered, Retrospective, Observational Study

( Jun Feng ),( Bangjiang Fang ),( Daixing Zhou ),( Junshuai Wang ),( Dengxiu Zou ),( Gang Yu ),( Yikuan Fen ),( Dan Peng ),( Jifa Hu ),( Daqian Zhan ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3

The coronavirus disease 2019 (COVID-19) pandemic has become a public health emergency of global concern. In China, traditional Chinese medicine has been widely administered to COVID-19 patients without sufficient evidence. To evaluate the efficacy of Shenhuang Granule (SHG) for treating critically ill patients with COVID-19, we included in this study 118 patients who were admitted to the ICU of Tongji Hospital between January 28, 2020 and March 28, 2020. Among these patients, 33 (27.9%) received standard care plus SHG (treatment group) and 85 (72.1%) received standard care alone (control group). Enrolled patients had a median (IQR) age of 68 (57-75) years, and most (79 [67.1%]) were men. At end point of this study, 83 (70.3%) had died in ICU, 29 (24.5%) had been discharged from ICU, and 6 patients (5.2%) were still in ICU. Compared with control group, mortality was significantly lower in treatment group (45.4% vs. 80%, p < .001). Patients in treatment group were less likely to develop acute respiratory distress syndrome (ARDS) (12 [36.3%] vs. 54 [63.5%], p = 0.012) and cardiac injury (5 [15.1%] vs. 32 [37.6%], p = 0.026), and less likely to receive mechanical ventilation (22 [66.7%] vs. 72 [84.7%], p = 0.028) than those in control group. The median time from ICU admission to discharge was shorter in treatment group (32 [20-73] days vs. 76 [63-79] days, p = 0.0074). These findings suggest that SHG treatment as a complementary therapy might be effective for critically ill adults with COVID-19 and warrant further clinical trials.