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Human Liver Specific Transcriptional Factor TCP10L Binds to MAD4
( Dao Jun Jiang ),( Hong Xiu Yu ),( Sa Yin Hexige ),( Ze Kun Guo ),( Xiang Wang ),( Li Jie Ma ),( Zheng Chen ),( Shou Yuan Zhao ),( Long Yu ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCPIOL in human hepatocarcinomas.
Human Liver Specific Transcriptional Factor TCP10L Binds to MAD4
Jiang, Dao-Jun,Yu, Hong-Xiu,Hexige, Sa-Yin,Guo, Ze-Kun,Wang, Xiang,Ma, Li-Jie,Chen, Zheng,Zhao, Shou-Yuan,Yu, Long Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.4
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.
Experimental study on performance of steam-water injector with central water nozzle arrangement
Wen Jun Li,Dao Tong Chong,Jun Jie Yan,Wei Xiong Chen,Jin Shi Wang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9
The steam-water injector (SI) is a simple mechanical device that has been widely used in industry. Wedid an experimental study to find the influence of physical and geometrical parameters on performance of the SI. Thephysical parameters studied were steam inlet pressure, water inlet pressure and water inlet temperature. Whereas, thegeometrical parameters studied were steam nozzle area ratio, area ratio of steam nozzle to water nozzle and the mixingsection converging angle. Pump head was introduced to evaluate the lifting-pressure performance of the SI under dif-ferent operating and geometrical conditions. Optimal values of steam nozzle area ratio and mixing section convergingangle were 1.3 and 11.6orespectively, for the present work, and optimal value of area ratio of steam nozzle to waternozzle increased with increasing water inlet pressure. Two head-capacity curves were introduced to highlight the effectof various physical and geometrical parameters on the performance of SI.
Proper efficiency for set-valued optimization problems and vector variational-like inequalities
Xian Jun Long,Jing Quan,Dao-Jun Wen 대한수학회 2013 대한수학회보 Vol.50 No.3
The purpose of this paper is to establish some relationships between proper efficiency of set-valued optimization problems and proper efficiency of vector variational-like inequalities under the assumptions of generalized cone-preinvexity. Our results extend and improve the corresponding results in the literature.
Oh, Hyo-Jun,Umapathi, Reddicherla,Omelianovych, Oleksii,Dao, Van-Duong,Jeong, Jun-Kyo,Lee, Ga-Won,Choi, Ho-Suk Elsevier S.A. 2019 Thin Solid Films Vol.676 No.-
<P><B>Abstract</B></P> <P>Dry plasma reduction under atmospheric pressure is a unique approach for stably, continuously, easily, and uniformly fabricating Ag layer on the metal seeds-polyethylene terephthalate (PET) substrate. In this study, effects of particle size, surface uniformity, surface coverage, and film thickness of metal seeds such as Ag, Cu, Fe and Ni on the formation of uniform Ag layer were studied on the basis of their surface free energies and critical radius, which further leads to variations in the sheet resistance levels of the silver layers. The root mean square roughness and sheet resistance of the Ag layer PET substrate were decreased with the deposition of metal seeds. Among the added seed metals, Ni seed has shown best uniformity. Furthermore, the fabricated films were applied for electromagnetic interference (EMI) shielding. Among the added seed metals, Ni has shown the highest electromagnetic interference shielding effectiveness. The present study will be useful for the development of various low-cost metal films in the field of EMI shielding.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform silver layer successfully fabricated with the aid of dry plasma reduction. </LI> <LI> Fabricated films were applied as electromagnetic interference shielding materials. </LI> <LI> Surface of silver layer formed more smoothly and uniformly by applying metal seeds. </LI> <LI> The findings may be useful for the development of various low-cost metal films. </LI> </UL> </P>
PROPER EFFICIENCY FOR SET-VALUED OPTIMIZATION PROBLEMS AND VECTOR VARIATIONAL-LIKE INEQUALITIES
Long, Xian Jun,Quan, Jing,Wen, Dao-Jun Korean Mathematical Society 2013 대한수학회보 Vol.50 No.3
The purpose of this paper is to establish some relationships between proper efficiency of set-valued optimization problems and proper efficiency of vector variational-like inequalities under the assumptions of generalized cone-preinvexity. Our results extend and improve the corresponding results in the literature.
Effect of cell senescence on the impedance measurement of adipose tissue-derived stem cells
Jun, H.S.,Dao, L.T.M.,Pyun, J.C.,Cho, S. IPC Science and Technology Press ; Elsevier Scienc 2013 Enzyme and microbial technology Vol.53 No.5
Label-free and real-time monitoring of stem cells based on electrical impedance measurement is increasingly utilized for the quality control of the isolated stem cells to be used in stem cell-based tissue therapy or regenerative medicine. In spite of that the proliferative capacity and multipotency of stem cells are dependent on the type and age of the source tissue, however, the effect of the cell senescence on the impedance measurement of stem cells has not yet been studied. We investigated whether the senescence of adipose tissue-derived stem cells (ADSCs) can be detected by electrical impedance spectroscopy. For this, ADSCs at passage 9 and 31 were prepared and those genetic characteristics and growth kinetics were evaluated by quantitative polymerase chain reaction and cell counting. While the identified ADSCs were grown on the indium tin oxide electrodes, the impedance spectra were measured and interpreted by fitting analysis with an equivalent circuit model. ADSCs at passage 9 adhered on the electrode were small and spindle-shaped whereas the cells at passage 31 were flattened and larger than younger cells. At the beginning of culture time when the cell adhesion occurred, the resistance at 4.6kHz of passage 31 cells was higher than passage 9 due to the larger size of older cells. Afterwards, the value of passage 9 cells increased higher than passage 31, since younger cells proliferated more than old cells. Therefore, the impedance measurement could characterize the proliferative capacity of ADSCs during expanded culture.
Dao Sik Son(孫斗植),Ka Aeng Ha(河佳鶯),Sang Jun Park(朴相俊),Jae Woo Hwang(黃在寓),Sang Rak Lee(李相錄) 한국육종학회 1992 한국육종학회지 Vol.24 No.3
The area of red pine forest stand damaged by pine gall midge insect has been wide spreading in the country. The damage causes a red pine tree to severe growth reduction and sometimes withering. To prevent the damage, it is needed to improve resistance of pinus densiflora to pine gall midge by introducing resistant factor. To see whether natural hybrid of P. densiflora and P. thunbergii founded in the area of coastal region in Kyungpook have fair resistance to the insect, the rate of growth reduction and gall forming on the needle were observed and the results were compared with those of parent species as follows. 1. The mean annual growth rates of ten year period before and after the year of 1983 were compared. That is, the comparison of mean annual growth rate of ten years period without and with pine gall midge damage, the growth reduction in P. densiflora was twice higher than P. thunbergii and their natural hybrid shows intermediate value of those parent species, i. e. the decrease of growth after attack to mean of annual growth of 10 years before attack in height and DBH was 70-80% and 40-70% in P. densiflora, 30-40% and 30-40% in P. thunbergii, 40-60% and 50-60% in hybrid, respectively. 2. Though P. densiflora has more needles containing pine gall midge eggs than P. thunberii by 15%, the rate of gall forming from them was 51.4% in P. densiflora and 21.5% in P. thunbergii. That is twice higher rate of gall forming in P. densiflora compare to P. thunbergii. 3. When the damage were classified into three grades, heavy, medium and light in mixed forest of two species, the value of above three grades were 32%, 67%, 1% in P. densiflora and 2%, 89%, 9% in P. thunbergii respectively.
Dao, Lan T.M.,Park, Eun-Young,Lim, Sang-Min,Choi, Yong-Soo,Jung, Hye Seung,Jun, Hee-Sook by Lippincott Williams Wilkins 2014 Transplantation Vol.98 No.10
BACKGROUND: Periosteum-derived progenitor cells (PDPCs) isolated from the adult periosteum can differentiate into several specific cell types. In this study, we examined the characteristics of human PDPCs and insulin-producing cells (IPCs) differentiated from PDPCs and their ability to ameliorate hyperglycemia when transplanted into streptozotocin-induced nonobese diabetic–severe combined immunodeficiency diabetic mice. METHODS: Periosteum-derived progenitor cells were isolated from patients, expanded in culture, and subjected to a three-step differentiation protocol to produce IPCs. The expression of immunogenic, pluripotent, and pancreatic markers was examined, and glucose-stimulated insulin release in vitro was also assessed. Insulin-producing cells that differentiated from PDPCs were transplanted under the kidney capsule of streptozotocin-induced diabetic mice, and glucose levels and glucose tolerance were measured. RESULTS: We found that PDPCs expressed the mesenchymal stem cell markers CD73, CD90, and CD105 and the pluripotent markers, octamer-binding transcription factor 4 and Nanog, but not sex-determining region Y-box 2 or Rex1. Periosteum-derived progenitor cells expressed human leukocyte antigen–ABC but did not express human leukocyte antigen–DR or the costimulatory molecules CD80 and CD86. Differentiated IPCs expressed pancreatic hormones (insulin, glucagon, somatostatin, and glucose transporter 2), hormone processing, and secretion molecules (prohormone convertase-1 and convertase-2, Kir6.2), and pancreatic transcription factors (neurogenin 3, pancreatic and duodenal homeobox 1, sex-determining region Y-box 17). When IPCs were stimulated with glucose in vitro, insulin secretion was elevated. Transplantation of IPCs under the kidney capsules of diabetic mice improved hyperglycemia and glucose tolerance. Human insulin was detected in the serum and kidney sections of mice transplanted with IPCs differentiated from PDPCs. CONCLUSION: These results suggest that IPCs differentiated from PDPCs might be an alternative source of &bgr; cells for treating diabetes.