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Song, Bang Ho,Fuente, Veronica De La,Glaser, Philippe,Danchin, Antoine 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
A spectionmycin cassette harboring the I-SceI meganuclease recognition site was inserted into the open reading frame of the Bacillus subtilis narA gene. This fusion vector was integrated into the narA locus of the chromosome and selected by marker rescue with spectinomycin resistance. Subsequent integration of the I-SceI site near the gerB region was performed in B. subtilis (pSO19) which already had the I-SceI ate in the narA gene. The transformant, B. subtilis (pSO19/20), containing I-SceI sites both in the locus narA and in the vicinity of gerB was isolated by selection with spectinomycin and chloramphenicol resistance. Direct isolation of the 70 kbp of narA-gerB stretch from the nested chromosome embedded in an agar plug was obtained by pulsed field gel electrophoresis after cutting both I-SceI sites. By this method, the precise location of these genes and the distance between gerB and narA were determined This unique method is very useful and applicable as a biochemical tool for isolating any intended fragments, for blocking specific loci Eke ate directed mutagenesis or large deletion, and for precise mapping of unknown genes by determination of their chromosomal distance and orientation.
Song, Bang Ho,Fuente, Veronica De La,Glaser, Philippe,Danchin, Antoine 경북대학교 유전공학연구소 1996 遺傳工學硏究所報 Vol.11 No.1
A spectionmycin cassette harboring the I-SceI meganuclease recognition site was inserted into the open reading frame of the Bacillus subtilis narA gene. This fusion vector was integrated into the narA locus of the chromosome and selected by marker rescue with spectinomycin resistance. Subsequent integration of the I-SceI site near the gerB region was performed in B. subtilis (pSO19) which already had the I-SceI site in the narA gene. The transformant, B. subtilis (pSO19/20), containing I-SceI sites both in the locus narA and in the vicinity of gerB was isolated by selection with spectinomycin and chloramphenicol resistance. Direct isolation of the 70 kbp of narA-gerB stretch from the nested chromosome embedded in an agar plug was obtained by pulsed field gel electrophoresis after cutting both I-SceI sites. By this method, the precise location of these genes and the distance between gerB and narA were determined. This unique method is very useful and applicable as a biochemical tool for isolating any intended fragments, for blocking specific loci like site directed mutagenesis or large deletion, and for precise mapping of unknown genes by determination of their chromosomal distance and orientation.
The contribution of microbial biotechnology to sustainable development goals
Timmis, Kenneth,de Vos, Willem M.,Ramos, Juan Luis,Vlaeminck, Siegfried E.,Prieto, Auxiliadora,Danchin, Antoine,Verstraete, Willy,de Lorenzo, Victor,Lee, Sang Yup,Brü,ssow, Harald,Timmis, James Ke John Wiley and Sons Inc. 2017 MICROBIAL BIOTECHNOLOGY -BLACKWELL- Vol.10 No.5
<P>The signature and almost unique characteristic of microbial technology is the exceptional diversity of applications it can address, and the exceptional range of human activities and needs to which it is and can be applied. Precisely because sustainability goals have very diverse and complex components and requirements, microbial technology has the ability to contribute substantively on many levels in many arenas to global efforts to achieve sustainability. Indeed, microbial technology could be viewed as a unifying element in our progress towards sustainability. </P>
Toward unrestricted use of public genomic data
Amann, Rudolf I.,Baichoo, Shakuntala,Blencowe, Benjamin J.,Bork, Peer,Borodovsky, Mark,Brooksbank, Cath,Chain, Patrick S. G.,Colwell, Rita R.,Daffonchio, Daniele G.,Danchin, Antoine,de Lorenzo, Victor American Association for the Advancement of Scienc 2019 Science Vol.363 No.6425
<P>Despite some notable progress in data sharing policies and practices, restrictions are still often placed on the open and unconditional use of various genomic data after they have received official approval for release to the public domain or to public databases. These restrictions, which often conflict with the terms and conditions of the funding bodies who supported the release of those data for the benefit of the scientific community and society, are perpetuated by the lack of clear guiding rules for data usage. Existing guidelines for data released to the public domain recognize but fail to resolve tensions between the importance of free and unconditional use of these data and the “right” of the data producers to the first publication. This self-contradiction has resulted in a loophole that allows different interpretations and a continuous debate between data producers and data users on the use of public data. We argue that the publicly available data should be treated as open data, a shared resource with unrestricted use for analysis, interpretation, and publication.</P>
Genomic Encyclopedia of Bacteria and Archaea: Sequencing a Myriad of Type Strains
Kyrpides, Nikos C.,Hugenholtz, Philip,Eisen, Jonathan A.,Woyke, Tanja,Gö,ker, Markus,Parker, Charles T.,Amann, Rudolf,Beck, Brian J.,Chain, Patrick S. G.,Chun, Jongsik,Colwell, Rita R.,Danchin, An Public Library of Science 2014 PLoS biology Vol.12 No.8
<▼1><P>This manuscript calls for an international effort to generate a comprehensive catalog from genome sequences of all the archaeal and bacterial type strains.</P></▼1><▼2><P>Microbes hold the key to life. They hold the secrets to our past (as the descendants of the earliest forms of life) and the prospects for our future (as we mine their genes for solutions to some of the planet's most pressing problems, from global warming to antibiotic resistance). However, the piecemeal approach that has defined efforts to study microbial genetic diversity for over 20 years and in over 30,000 genome projects risks squandering that promise. These efforts have covered less than 20% of the diversity of the cultured archaeal and bacterial species, which represent just 15% of the overall known prokaryotic diversity. Here we call for the funding of a systematic effort to produce a comprehensive genomic catalog of all cultured Bacteria and Archaea by sequencing, where available, the type strain of each species with a validly published name (currently∼11,000). This effort will provide an unprecedented level of coverage of our planet's genetic diversity, allow for the large-scale discovery of novel genes and functions, and lead to an improved understanding of microbial evolution and function in the environment.</P></▼2>