Genetic variation of Colletotrichum magnum isolated from Carica papaya as revealed by DNA fingerprinting

Daisy Pérez-Brito,Alberto Cortes-Velázquez,Teresita Valencia-Yah,Anuar Magaña-Álvarez,Cuauhtémoc Navarro,Blanca Moreno,Steven Quiroga,Raúl Tapia-Tussell 한국미생물학회 2018 The journal of microbiology Vol.56 No.11

Mexico is one of the five largest producers of papaya worldwide, but losses caused by pathogens, mainly fungus, at the pre- and post-harvest stages are often more than 50% of the crop. Papaya anthracnose, caused by three different species of the Colletotrichum genus in Mexico, occupies a preponderant place in this problem. Although two of these species, C. gloeosporiodes and C. truncatum, have been characterized morphologically and genotypically, this has not occurred with C. magnum, the third species involved, about which there is very little information. Because of this, it is vital to know its genetic characterization, much more so considering that the studies carried out on the other two species reveal a wide genetic diversity, differences in pathogenicity and in the response to fungicides of the different strains characterized. In this work, Colletotrichum spp. isolates were collected at different papaya orchards in the south-southeast of Mexico. C. magnum isolates identified by species-specific primers were characterized by morphological and molecular approaches. Differences in colony characteristics resulted in five morphological groups. AP-PCR, DAMD and ISSR markers were found to be very efficient for revealing the interspecific variability of this species. The high genetic variability found in the accessions of C. magnum was linked to the geographical area where they were collected. Isolates from Chiapas State were the most variable, showing point mutations in the ITS1- ITS2 region. These results will enable a better phytosanitary management of anthracnose in papaya in this region of Mexico.

Effect of D-003 on Isoproterenol-Induced Myocardial Necrosis in Rats

Daisy Carbajal,Miriam Noa,Vivian Molina,Maria Lourdes Arruzazabala,Rosa M?,Sarah?Mendoza,Jorge Gonz?ez 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.1

D-003 is a mixture of high-molecular-weigh t aliphatic primary acids purified from sugar canewax with antiplatelet and cholesterol-lowering effects. Cardiac lesions induced by isopro-terenol (ISO) are characterized by myocardial necrosis and exudative infiltration. The objec-tive of this study was to determine whether D-003 shows protective effects against ISO-in-duced myocardial necrosis in rats. Effects of orally administered single doses of D-003 (25 40mg/kg) and acetylsalicylic acid (ASA, 30 mg/kg), as well as repeated doses of D-003 (5 20mg/kg), on characteristic markers of ISO-induced myocardial necrosis in rats were investi-gated. D-003 administered as single doses dose-dependen tly decreased necrosis area, percentof infarct area, and the presence of polymorphonuclear cells (PMNs) in myocardial tissue, butonly the reductions induced by 200 and 400 mg/kg were significant. Oral acute treatment withASA also decreased necrosis area and percent of infarct area, but the occurrence of PMNs wasunchanged. D-003 administered repeatedly for 10 days also decreased all myocardial necro-sis indicators in a dose-dependen t maner, with results effective from 25 mg/kg to the high-est dose tested, indicating that the repeated dose scheme was more effective to prevent thedamage. It is concluded that D-003 shows a protective effect on the myocardial necrosis in-duced by ISO in rats.13

THE EFFECT OF FEAR-OF-MISSING-OUT (FOMO) LADEN INFLUENCER MARKETING ON TRAVEL DESTINATION PROMOTION

Daisy Lee,Tai Ming Wut,Calvin Wan 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07

Fear of missing out (FOMO) refers to the customer's perception of being anxious for not engaging in an experience. FOMO is an anxiety feeling positively associated with social media usage that one cannot catch up on something important in life. Fear of missing out (FOMO) marketing appeals initiated from social media usage were found to significantly affect consumer purchase decisions including choice of destination. Consumers usually browse social media and social networking sites such as forums and reviews in online tourism agents (OTAs) when they make travel decisions. Although FOMO is expected to affect tourists' perception and urgency in making a tourism decision, the use of FOMO-laden message to promote travel destination through different types of influencers has not yet been widely studied. This study fills this research gap by examining the effect of using FOMO laden content to promote travel destination through different types of influencers. An online experiment was conducted with four experimental conditions in which different influencers share about a destination using the same FOMO-laden message: (1) travel KOL, (2) tourists who post user-generated-content (UGC), (3) personal friends, and (4) a control condition with the absence of influencer and FOMO message. The 984 respondents were randomly assigned into one of the four experimental conditions. Data collected was analysed using PLS-SEM and PLS-MGA. Results indicated that anticipated elation, anticipated envy, and social influence predicted 30.2% variance of FOMO and FOMO explained 31.6% of variance of intention to visit the destination promoted. Multi-group analysis (PLS-MGA) found that exposure to message shared by travel KOL and personal friends significantly strengthen the FOMO feeling of participants resulting in strong intention to visit the destination promoted. UGC posted by tourists showed similar effect as the non-FOMO laden control group and are less significant in driving the FOMO feeling that leads to visit intention. Findings of this study provide insights into how effectiveness of destination promotion can be enhanced by using FOMO-laden message on social media through influential influencers.

Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting

Daisy Pérez-Brito,Anuar Magaña-Alvarez,Patricia Lappe-Oliveras,Alberto Cortes-Velazquez,Claudia Torres-Calzada,Teófilo Herrera- Suarez,Alfonso Larqué-Saavedra,Raul Tapia-Tussell 한국미생물학회 2015 The journal of microbiology Vol.53 No.1

This study characterized Clavispora lusitaniae strains isolatedfrom different stages of the processing and early fermentationof a henequen (Agave fourcroydes) spirit produced inYucatan, Mexico using a molecular technique. Sixteen strainsidentified based on morphological features, obtained fromdifferent substrates, were typed molecularly. Nine differentversions of the divergent D1/D2 domain of the large-subunitribosomal DNA sequence were identified among theC. lusitaniae strains. The greatest degree of polymorphismwas found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% ofthe isolates. This study provides significant insight into thegenetic diversity of the mycobiota present during the henequenfermentation process, especially that of C. lusitaniae,for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealingpolymorphisms between isolates of this species.

Sequence Analysis and Potential Action of Eukaryotic Type Protein Kinase from Streptomyces Coelicolor A3(2)

Daisy R. Roy,Sathees B.C.Chandra 한국유전체학회 2008 Genomics & informatics Vol.6 No.1

Protein kinase C (PKC) is a family of kinases involved in the transduction of cellular signals that promote lipid hydrolysis. PKC plays a pivotal role in mediating cellular responses to extracellular stimuli involved in proliferation, differentiation and apoptosis. Comparative analysis of the PKC-α,β,ε isozymes of 200 recently sequenced microbial genomes was carried out using variety of bioinformatics tools. Diversity and evolution of PKC was determined by sequence alignment. The ser/thr protein kinases of Streptomyces coelicolor A3 (2) , is the only bacteria to show sequence alignment score greater than 30% with all the three PKC isotypes in the sequence alignment. S.coelicolor is the subject of our interest because it is notable for the production of pharmaceutically useful compounds including anti-tumor agents, immunosupressants and over two-thirds of all natural antibiotics currently available. The comparative analysis of three human isotypes of PKC and Serine/ threonine protein kinase of S.coelicolor was carried out and possible mechanism of action of PKC was derived. Our analysis indicates that Serine/ threonine protein kinase from S. coelicolor can be a good candidate for potent anti-tumor agent. The presence of three representative isotypes of the PKC super family in this organism helps us to understand the mechanism of PKC from evolutionary perspective.

Self-evaluated knowledge of pharmacy customers in South-Estonia about the use and safety of herbal products

Daisy Volmer,John Lilja,David Hamilton 셀메드 세포교정의약학회 2011 TANG Vol.1 No.1

Medicinal plants and their products are popular in Estonia. There are two approaches to use of herbal products: first, that based on traditions and practical experience and, second, that supported by scientific evidence. It is important to marry these two approaches. One place where traditions and new knowledge could meet is the pharmacy. In this study we evaluated knowledge about the use and safety of herbal products of pharmacy customers in South-Estonia. A convenience sample of pharmacy customers in south Estonia (n = 196) participated in the study. Of the survey participants, 76% were frequent or occasional users of herbal products and considered these products safe (75%) and effective (73%). Herbal products were mostly (91%) consumed for prophylaxis or treatment of minor illnesses. Main information sources about herbal products were pharmacists (75%) and package information leaflets (65%). Mode of action (95%), administration (81%) and indication (77%) were the information details more frequently sought from the pharmacy about herbal products. Of the survey participants, 22% described some problems connected with the use of herbal products. Herbal products are popular in Estonia and pharmacists have an important role in counselling on these products. Despite knowledgeable use of herbal products and infrequent experience of side effects with these products, safety issues should be more stressed in the providing of information details to pharmacy customers.

오늘은 바게트

Daisy Kim 고요아침 2023 열린시학 Vol.28 No.4

Evolving the Cybersecurity of Clinical Photography in Plastic Surgery

Spoer Daisy L.,Junn Alexandra,Bovill John D.,Haffner Zoë K.,Abadeer Andrew I.,Baker Stephen B. 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.4

Point-of-care photography and photo sharing optimize patient outcomes and facilitate remote consultation imperative for resident surgeons. This literature review and external pilot survey study highlight the risks associated with current practices concerning patient privacy and biometric security. In a survey of 30 plastic surgeon residents and attendings, we found that themajority took photos of patients with their iPhones and shared them with colleagues via Apple iMessage. These findings corroborate previous reports and highlight a lack of physician user acceptance of secure photosharing platforms. Finally, we frame a successful example from the literature in the context of a postulated framework for institutional change. Prioritizing the privacy and safety of patients requires a strategic approach that preserves the ease and frequency of use of current practices.

An optimized index for cold tolerance assessment in rice during germination and early seedling stage

Monzón Daisy Leticia Ramirez,Cantero Jorge,Danielowski Rodrigo,da Luz Viviane Kopp,Venske Eduardo,Mota Monalize Salete,da Silva Raissa Martins,de Oliveira Victoria Freitas,de Oliveira Antonio Costa,de 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2

Cold tolerance at germination and seedling stage is one of the most seeked traits in Southern Brazilian rice (Oryza sativa L.) cultivars, thus, the availability of improved methods for germplasm assessment is of high importance for breeding programs. The aim of this study was to validate an optimized Average Tolerance Index (ATI) for rice germplasm assessment under cold at germination and early seedling stage. A diverse panel composed by 124 rice accessions was assayed. Four genotypes with known performance under cold were used as controls (two tolerant and two sensitive). The genotypes were sown in roll papers, and evaluated at 13 °C for 28 days (cold stress) and 25 °C for seven days (control). Four traits were measured, germination percentage, coleoptile length, root length and shoot length, which were converted in terms of relative perfor‑ mance (cold/control). A PCA analysis was carried out. The ATI was calculated as a linear combination of all trait’s relative performance, weighted by the importance of each trait in explaining the genetic variability in the set, through including the PC1 vectors as trait coefcients. K means was applied for genotype classifcation. The panel showed expressive genetic variability for performance under low temperature. The ATI successfully distinguished the controls regarding their cold tolerance and allowed the formation of four classes of genotypes, sensitive, moderately sensitive, moderately tolerant and tolerant. The assessment of rice germplasm for cold tolerance at germination and seedling stage through ATI is a viable alternative for the identifcation of tolerant genotypes.

Sequence Analysis and Potential Action of Eukaryotic Type Protein Kinase from Streptomyces coelicolor A3(2)

Roy, Daisy R.,Chandra, Sathees B.C. Korea Genome Organization 2008 Genomics & informatics Vol.6 No.1

Protein kinase C (PKC) is a family of kinases involved in the transduction of cellular signals that promote lipid hydrolysis. PKC plays a pivotal role in mediating cellular responses to extracellular stimuli involved in proliferation, differentiation and apoptosis. Comparative analysis of the PKC-${\alpha},{\beta},{\varepsilon}$ isozymes of 200 recently sequenced microbial genomes was carried out using variety of bioinformatics tools. Diversity and evolution of PKC was determined by sequence alignment. The ser/thr protein kinases of Streptomyces coelicolor A3 (2), is the only bacteria to show sequence alignment score greater than 30% with all the three PKC isotypes in the sequence alignment. S.coelicolor is the subject of our interest because it is notable for the production of pharmaceutically useful compounds including anti-tumor agents, immunosupressants and over two-thirds of all natural antibiotics currently available. The comparative analysis of three human isotypes of PKC and Serine/threonine protein kinase of S.coelicolor was carried out and possible mechanism of action of PKC was derived. Our analysis indicates that Serine/ threonine protein kinase from S. coelicolor can be a good candidate for potent anti-tumor agent. The presence of three representative isotypes of the PKC super family in this organism helps us to understand the mechanism of PKC from evolutionary perspective.