Antispasmodic Effects of Junsibaekchul-San In Vivo and In vitro

Hur, Jin-Il,Byun, Joon-Seok,Kim, Dae-Jun The Physiological Society of Korean Medicine and T 2010 동의생리병리학회지 Vol.24 No.1

In Vivo and In vitro antispasmodic effects of Jun-Si-Baek-Chul-San, a Traditional Korean Polyherbal Medicineconsisted of 7 types of herbs were observed in the present study. To clarify the effects of Jun-Si-Baek-Chul-San, on accelerating small intestinal movement induced by the stimulation of cholinergic neurotransmission, we evaluated the effects of Jun-Si-Baek-Chul-San on In vivo carbachol (an acetylcholinergic agent)-accelerated mice small intestinal transit and on In vitro contractions induced by low-frequency electrostimulation, KCl, histamine or acetylcholine using isolated guinea pig ileum. To induce the acceleration of mice small intestinal transit, Carbachol 1 mg/kg was once subcutaneously dosed 15min before last administration of the test drugs. In the present study, Jun-Si-Baek-Chul-San 500, 250 and 125 mg/kg or domperidone 20 mg/kg were orally pretreated on the carbachol-accelerated mice small intestinal transit once a day for 7 days and the small intestinal transit rateof activated charcoal powder were monitored. In vitro assays, Jun-Si-Baek-Chul-San1, 0.1, 0.01 and 0.001 mg/ml or domperidone $2{\times}10^{-5}M$ were treated 10min before ileal contraction was induced by filed stimulation, acetylcholine, KCl and histamine, and the % changes of contractions were observed compared to the treatment of inducer alone. In spontaneous contraction, the % changes of contractions were observed compared to treatment of vehicle alone at 10min after Jun-Si-Baek-Chul-San or domperidone treatment. The efficacy of Jun-Si-Baek-Chul-San was compared to those of domperidone. High concentration, 1 mg/ml of Jun-Si-Baek-Chul-San was found to decrease the spontaneous contraction of the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San decrease contractions induced by electrostimulation, acetylcholine, histamine and KCl in the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San effectively inhibited the accelerated small intestinal movement induced by carbachol stimulation of cholinergic neurotransmission in In vivo. Based on the results, although the exact molecular or action mechanism and which herbs or compound in Jun-Si-Baek-Chul-San are responsible for actions, it was concluded that Jun-Si-Baek-Chul-San normalization in the accelerated intestinal motility might be interfere with a variety of muscarinic, adrenergic and histaminic receptor activities or with the mobilization of calcium ions required for smooth muscle contraction non-specificly. Therefore, it is expected that Jun-Si-Baek-Chul-San will be promising as a prescription of clinical treatment of digestive tract disorders such as accelerated the motility of intestine, diarrhea or intestinal painful contractions.

보성산 유기농 녹차의 품질에 따른 카테킨 함량과 항산화능 비교 분석

박경련(Kyung Ryun Park),이상길(Sang Gil Lee),남태규(Tae Gyu Nam),김영준(Young Jun Kim),김영록(Young-Rok Kim),김대옥(Dae-Ok Kim) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.1

유기농 녹차의 등급별 총페놀 함량과 항산화능은 각 추출 용매에 따른 수율을 확인하였을 때 물 추출에 비하여 수용성 유기용매의 추출 수율이 유의적으로 높았다. 유기용매를 이용한 추출수율은 물을 이용할 때 보다 총페놀 함량은 1.5에서 3.2배, 항산화능은 1.8에서 3.8배 정도 높게 나타났다. HPLC 분석을 통하여 총페놀 함량과 항산화능의 상관관계를 비교 시 총페놀 함량이 증가함에 따라 항산화능이 1차 선형관계로 증가하는 경향을 보였다. HPLC분석을 통하여 유기농 녹차에 함유되어 있는 다양한 카테킨의 함량이 채엽시기에 따라 변화가 나타났다. 유기농 녹차의 카테킨 성분 중 가장 많이 존재하는 EGCG가 건조중량당 5.8-7.7%의 함량을 보였고, caffeine은 1.7-2.9%의 함량을 보였다. 유기농 녹차의 카테킨 함량은 일반적으로 채엽 시기가 늦어짐에 따라서 점차 감소하였다. 유기농 녹차의 등급에 따른 항산화능은 최상급인 우전에서 가장 높았으며 최하등급인 엽차에서 가장 낮게 나타났다. HPLC 분석에 의한 유기농 녹차의 등급에 따른 총 카테킨 함량(㎎/g)은 우전(155.4), 세작(147.7), 중작(143.2), 엽차(135.1), 대작(130.5)의 순서로 감소하였다. 이를 통해 녹차의 채엽 시기가 유기농 녹차의 카테킨 성분 및 항산화능에도 영향을 미치는 것을 확인하였다. 전반적으로 상급의 유기농 녹차 일수록 총페놀 및 카테킨 함량이 많으며 또한 더 높은 항산화능을 가지는 것으로 나타났다. The objective of this study was to evaluate the effect of various solvents on extraction of bioactive phenolics and to analyze the antioxidant capacity and contents of individual catechins in various grades of green teas organically grown in Boseong, Korea. The organic green teas, based on their harvest seasons, were categorized into five grades such as Woo-Jeon, Se-Jak, Jung-Jak, Dae-Jak, and coarse tea. Solvents used to extract phenolics from these teas included water at 23℃ and 70℃ as well as 80% (v/v) aqueous methanol and ethanol. In general, aqueous organic solvents of methanol and ethanol led to higher extraction yields of phenolics than water at 23℃ and 70℃. Total phenolics and antioxidant capacity of the teas extracted with the aqueous organic solvents were approximately 1.5 to 3.2 and 1.8 to 3.8 times higher than those with water at 23℃ and 70℃, respectively. Coarse tea, the lowest grade of green tea, showed approximately 30-60% lower total phenolics and antioxidant capacity compared with the higher grade ones. Reversed-phase HPLC analysis was performed quantitatively to identify individual catechins, gallic acid, and caffeine in teas extracted with 80% (v/v) aqueous methanol. Based on their dry weights, the organic green teas contained about 1.7 to 2.9% of caffeine. Content (㎎/g dry weight) of tea catechins decreased in the following order: Woo-Jeon (155.4) > Se-Jak (147.7) > Jung-Jak (143.2) > coarse tea (135.1) > Dae-Jak (130.5). (-)-Epigall℃atechin gallate was the most abundant among the catechins analyzed. The highest grade of green tea, Woo-Jeon, had the highest amount of (-)-epigall℃atechin gallate at 77.4 ㎎/g dry weight. Overall, the higher grade of organic green teas tended to have the higher level of antioxidant capacity and catechins.

대구한의대 비만클리닉 내원 환자 62명에 대한 임상적 관찰

허진일,김동우,박경,김대준,변준석,최빈혜,Hur, Jin-Il,Kim, Dong-Woo,Park, Kyung,Kim, Dae-Jun,Byun, Joon-Seok,Choi, Bin-Hye 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.2

Objectives : The purposes of this study are to evaluate the effect of treatment in obesity clinic in Dae-Gu Hanny University and to improve the oriental medical methods of treatment in obesity patients. Methods : 62 patients were classified into four groups by Body Mass Index(BMI) : normal, overweight, 1st obesity and 2nd obesity groups. Weight, BMI, body fat rate and abdominal fat rate were compared in each group by t-test. Results : In distribution of sex, male were 10(16.1%), female were 52(83.9%). Mean age was $31.0{\pm}2.59$ and the patients in twenties and thirties were the most. Mean weight was $71.80{\pm}4.08kg$ and 1st obesity group(23) was the most. Mean BMI was $27.11{\pm}1.20(kg/m^2)$. BMI Changes in obesity groups treated by obesity clinic were $0.95{\pm}0.68kg/m^2$ in normal group, $1.45{\pm}0.38kg/m^2$ in overweight group, $2.67{\pm}0.48kg/m^2$ in 1st obesity group and $4.01{\pm}1.10kg/m^2$ in 2nd obesity group. The changes in all groups were significant. The more the obesity degree was. the more weight loss was. Conclusions : The weight and BMI in patients were almost decreased significantly when they treated. It resulted that the effect of oriental medical treatment in obesity was positive. This study is the effect of treatment in obesity by several medical methods and it is thought that the study of the effects of treatment in obesity by each medical methods that were used in this study is necessary.

소적정원산(消積正元算)으로 호전된 적증(積證)환자 1례 보고

허진일,조동희,김동우,임희용,오중한,김대준,변준석,최빈혜,Hur, Jin-Il,Cho, Dong-Hee,Kim, Dong-Woo,Lim, Hee-Yong,Oh, Jung-Han,Kim, Dae-Jun,Byun, Joon-Seok,Choi, Bin-Hye 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

This study was designed to evaluate the effects of the oriental medicine(Sojeockjeongwonsan) on a Jeock-Jeung patient. The clinical data was analyzed on a patient with Jeock-Jeung, deemed due to Gangiulgyul(肝氣鬱結) and Junggiheoyak(正氣虛弱). The patient’s main symptoms were abdominal pain and abdominal mass. The patient was admitted to Dae-Gu Hanny University Dae-Gu Oriental Medicine Hospital. Clinical symptoms, including abdominal pain, abdominal mass and general condition, improved after treatment. This study suggests that Sojeockjeongwonsan is significantly effective in treatment of a Jeock-Jeung.

모의지진동을 사용한 고층 건축물의 내진해석에서 탄소성 응답스펙트럼법의 적용

전대한 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

This paper examined various aspects of a linear and a nonlinear response spectrum method in seismic response analysis of biilding structures subjected tosimulated earthquake motions compatible design response spectra. The simulated motions has constant velocity response spectra with range of period from 0.5 to 5.0 seconds. The response spectrum method that has been widely used in seismic analysis of linear structures was proposed different mode superposition method by several ivestigators, and the differences between combination with an elastic modal analysis reviwed closely. It is the purpose of this paper to propose an alaternative method by means of which a nonlinear MIDOF structure with long period may be analysed by an extention of response spectrum method. For nonlinear seismic analysis of high-rise building structures using technique proposed in this study, it is intended to serve primarily as a tool in preliminary designs replace time history analysis.

관골골절의 새로운 외부견인법

전대우,한기환,강진성 大韓成形外科學會 1989 Archives of Plastic Surgery Vol.16 No.5

In the modern era of increasing motorization and industrialization, traffic and industrial accidents are becoming more frequent and serious, which in turn have brought about an increase in facial bone fractures and the pattern of fractures have become more compound and comminuted. The malar eminence is so prominent in oriental people that the zygomatic bone is more frequently fractured than other facial bones. Even a markedly depressed zygomatic fracture can be well reduced with three point interosseous wire fixations including a zygomaticomaxillary buttress, a late depression of the malar eminence occurs frequently due to the pull of the masseter muscle. In order to antagonize the pull of the masseter muscle the authors have devised a new method of external traction using an Ⅳ pole and weights which were all available on the wards. Fifty-seven patients who were admitted at Keimyung University Dong-San Medical Center from November 1987 to August 1988, were treated with 3 point interossous wire fixations and myotomy of the insertion of the masseter muscle. Of the 57, 32 were then managed with the authors' external traction method and the remaining 25 were managed with external traction using Georgiade's halo apparatus. The following results are obtained by comparing our method to Georgiade's : 1. Although the authors' external traction method tends to produce a slight overcorrection(P〉0.05), the results should be improved with experience. 2. Advantages of the authors method are as follows: 1) There is no cost because all needed equipments are obtainable on the wards. 2) The traction apparatus can be easily assembled. 3) It does not annoy the patients and does not necessitate scalp wounds. 4) It is useful for treatment of the patients who have concomitant cranial or cervical bone fractures. 3. Disadvantages are: 1) The authors method is contraindicated in the patient who is not cooperative because the direction of traction can be varied. 2) There is a brief limitation of sustained traction whenever the patient is moved. In summary, the authors external traction method using an IV pole and weights can be used alone and/or as aid to Georgiade's halo apparatus in the post-operative management of depressed zygomatic fractures.

고 탁도 건설 폐수 응집 특성 분석

이준호, 김명호, 고대현 韓國交通大學校 2020 한국교통대학교 논문집 Vol.55 No.-

In this study, we tried to find an efficient coagulation agent by comparing and analyzing the coagulation properties of each coagulation agent by conducting a Jar-test on various coagulation agents used for the efficient treatment of high turbidity construction wastewater. The Jar test was conducted with wastewater with a turbidity of 1,150 NTU and SS 1500 mg/L using yellow soil. PACS-2, PACSS, PACSM, PACS300, Chitosan, and Alum were tested in Jar-test and the turbidity, pH, SS, standing analysis, filtration time, etc. were analyzed to compare the cohesive characteristics of each floc. PACS-2 ranges from turbidity 36.9~124 NTU (average 82.24 NTU), pH 6.934~7.100 (average 7.025) and SS 20~70 mg/L (average 45.2 mg/L). PACSS appeared to range from turbidity 6.59~59.3 NTU (average 21.29 NTU), pH 5.166~7.010 (average 6.331), and SS 6~28 mg/L (average 13.6 mg/L). Also PACSM was found to range from turbidity 4.16~94 NTU (average 35.18 NTU), pH 5.742~6.908 (average 6.582), and SS 4~118 g/L (average 34 mg/L). PACS300 ranged from turbidity 5.22~85.7 NTU (average 31.07 NTU), pH 5.968~6.875 (average 6.603) and SS 4~66 mg/L (19.6 mg/L on average). Chitosan was found to be in the range of turbidity 7.3~50.1 NTU (19.58 NTU on average), pH 6.738~7.097 (6.900 on average), and SS 4~32 mg/L (13.6 mg/L on average). Finally, the Alum was found to range from turbidity 22~217 NTU (average 132.72 NTU), pH 6.878~7.113 (average 6.984), and SS 22~224 mg/L (average 115.2 mg/L). As a result of the experiment, Chitosan is considered to have a high coagulation characteristics as a coagulation agent of high turbidity construction wastewater due to low change in pH and excellent coagulation characteristics.

철근콘크리트 벽식 구조물에서 전단벽의 탄소성 해석용 모델화 방법의 검토

전대한 東西大學校 2002 동서논문집 Vol.8 No.-

In this paper a relatively simple and reliable wall models are investigated, which are suitable to be efficiently incorporated in a practical nonlinear seismic analysis of reinforced concrete shear wall structural systems. Four types of analogous frames have been selected for the elastic stress analysis. Three types of macro-elements model which include wide-column model, truss model and Kabeyasawa model, are chosen for the use in nonlinear analysis. A numerical analysis is carried out for six stories plane coupled wall structure. Analysis results indicate that macro-elements wall model is effective and suitable for simulating stress in elastic analysis. In inelastic analysis, the yielding strength have little effect on different wall model, and the effect on post-hielding stiffness in story shear-drift relationship depend on force-deformation properties of macro-elements.

등가 1자유도계에 의한 철근콘크리트 건물의 층 변위응답 산정

전대한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

To evaluate the seismic capacity of a multi story building structures in performance based seismic design, it is need to convert MDOF model into equivalent SDOF model. This paper presents predictions for story displacements of multistorey structures using method of converting a MDOF system into an equivalent SDOF model. The principal objective of this investigation is to evaluate appropriateness of converting method through perform nonlinear time history analysis of a multistory building structures and an equivalent SDOF model. Comparing the story displacements of multistorey structures calculated by time history response analysis and those evaluated by an equivalent SDOF model, the adequacy and the validity of converting method is verified. The conclusion of this study is following; A method of converting a MDOF system into an equivalent SDOF model through the nonlinear time history response analysis is valid. Inelastic first mode shapes are expected to be more accurate than elastic first mode shapes in obtaining story displacement of multistorey structures from equivalent SDOF model.

WIPI 기반 모바일 3D 게임엔진의 설계와 구현

형대진,김승구,조현준,박경환 동아대학교 정보기술연구소 2006 情報技術硏究所論文誌 Vol.14 No.1

In this paper, we introduce the method for developing the mobile 3D game engine based on the WIPI platform which has been proposed as the mobile standard platform. Our game engine is designed so that it supports OpenGL-ES and JSR-184 that are mobile 3D graphic APIs. Our game engine has a mobile emulator operated on PC and SDK to support mobile programming in PC environments. Mobile 3D game developers can use the APIs at various levels. Also, the game engine is developed in modular in order to support various game genres.