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      • 타이어의 동특성 분석을 위한 소프트웨어 개발

        尹大遠,朴鍾演,張牧淳,朴贊元 江原大學校 産業技術硏究所 1993 産業技術硏究 Vol.13 No.-

        To analyze dynamic characterics for a motorcar, the creating and the hammering test methods are introduced. This paper has studied the method of computing the natural frequency of the impulse wave on a tire, the decay constant which determines the absorption capability for the impulsive force. Moreover we have examined the reliability for the decay constant calculated, in this paper theoritical results are agreed with the simulated ones.

      • 실무개발자를 위한 BSD 소켓프로그래밍에 관한 연구

        최대석,권중장,최재원 경성대학교 공학기술연구소 2002 공학기술연구지 Vol.9 No.-

        네트워크 응용 프로그램을 개발하기 위해서는 Socket API를 많이 이용한다. Socket API를 이용해 정상적인 동작을 하는 것은 아주 쉽다. 그러나 실제 개발 과정에서 정상적인 상황이 아닌 여러 가지 비정상적인 오류에 처하게 됨에 따라 속수무책일 때가 많다. 따라서 본 논문에서 각 플랫폼별로 비정상적인 오류들의 사례 연구를 통하여 효율적인 응용시스템 개발에 도움을 주고자 한다.

      • RAPD(Random Amplified Polymorphic DNA)법을 이용한 한약재의 판별 연구

        김대원,김도균,안선경,조동욱 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

        Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition. Using these methods, however, is not satisfying for the precise identification of natural products because they are often variable in the compositions, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notohinseng, were genetically analyzed by RAPD. Also, DNA isolated form Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctors, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification of other natural products.

      • SCOPUSKCI등재

        Cyanobacterium Synechocystis PCC6803 내에서 Bacillus thuringiensis subsp. morrisoni PG-14 cryIVD 유전자의 발현

        이대원,박현우,진병래,정영호,박영목,강석권 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

        미생물 살충제로 사용되고 있는 B. thuringiensis를 모기유충방제에 적용하기 위한 시도는 B. thuringiensis가 수서생태계에서 부유하지 못하고 가라앉으며, 생활환을 이루지 못한다는 문제점으로 인해 적용에 어려움이 있다. 따라서 본 연구는 모기유충에 강한 독성을 갖는 B. thuringiensis subsp. morrisoni PG-14의 cryIVD 유전자를 모기유충의 먹이인 cyanobactrium에 도입하기 위하여 발현벡터 pCYASK 5-1을 제작하고, cyanobacterium Synechocystis PCC6803에 형질전환시켜, 세포내에서 cryIVD 유전자의 발현과 뇌염모기에 대한 독성을 조사하였다. 그 결과 형질전환체 내에서 cryIVD 발현은 immunoblot 분석을 통해 B. thuringiensis subsp. morrisoni PG-14에서 발현된 단백질과 같은 분자량으로 발현되는 것을 확인하였으며, 모기유충에 대한 형질전환체의 독성은 1.40×10^6 cells/ml 농도에서 약 80%의 치사율을 나타내었다. Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASk5-1 was transformed into the cyanobacterium Synechocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of cryIVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

      • 로봇 포도수확기를 위한 엔드이펙터 개발

        이대원,김현태,이용국 성균관대학교 생명공학연구소 2001 生命工學硏究 Vol.7 No.1

        Most of fruits need laboring greatly to harvest fruit in the period of harvest season. Grape also needs requiring a lot of labor to harvest in time, since harvesting of grape is cut and graped by hand. At abroad, especially, a harvester of grape, which is not eat freshly raw grape fruit but is processed to be made wine, was developed in France. However, a harvester of grape which can eat freshly raw grape fruit haven't not been developed. Therefore, this study was designed and constructed to develope an end effector of the robotic harvesting system. In order to find average and standard deviation of size and shape of a bunch grape, the grape farm was visited and investigated to find out the important factors for end effector to be designed and built. The efficient end-effecter should reach very well a bunch of grape without touching obstacles, such as leaf and stem in the grape vine. Also, it was made as simple, small and light as possible. Therefore, It was made of duralumin, which was lighter material than iron, to reach easily fresh grape fruit and reduce a torque. After finding the optimum initial speed, slew speed, ramp speed, the end effector proved to be a smooth operating end effector. For verifying efficiency of the end-effecter, the control board was designed and manufactured, and also developed the software to control the motors of the end effector.

      • 왕방산 남사면의 식물상에 관한 연구

        강대성,백원기 대진대학교 2002 大眞論叢 Vol.10 No.-

        This study was to establish the floristic composition of resources vascular plants of the southern slope area at Mt. Wangbang(736.3 m; 127˚ 08' 11" -127˚ 12' 19" E, 3 7˚ 51' 29" ~37˚ 55' 32" N). This work was investigated during the period of 1996 & 2002. Resources vascular plants were composed of 100 families, 301 genera, 409 species, 47 varieties and 9 formae, Totaling 465 taxa. Among the investigated resources plants, 7 taxa(l.2%) were Korean endemic species. The special plants based on floral region by Ministry of Environment(1999) were 27 taxa; Ⅳ rank species 2 taxa, Ⅲ rank species 4 taxa, Ⅱ rank species 4 taxa, Ⅰrank species 17 taxa. A naturalized plants were 44 species, correspond to 20.2% of totaling 218 species appeared in South Korea. Life form spectra was H-D₄-R_(5)-e type and, useful resources plants are as follows; edible source(45.2%), pasture source (41.9%), medicinal source(34.0%) and ornamental source(l6.1%) in the investigated region.

      • 다동온실의 복합환경 제어시스템을 위한 소프트웨어 개발

        이대원,김채웅 성균관대학교 생명공학연구소 1999 生命工學硏究 Vol.5 No.1

        A hybrid environmental control system was designed and built in the multi-greenhouses. After the hardware system was developed, the software was designed and constructed for this project. A software was adequate for reading data from many sensors and operating side windows, top windows, curtains and fans in the two greenhouses. Separate programs for control of the hardware, written Visual basic, Turbo-C and Assembler computer language, were developed to make the hybrid environmental control system operate. The program was a menu driven program within Windows environment. The initial menu was easily implemented by pressing a keypad key(KPK) on the keyboard or a mouse. Continued manipulation of the KPK or a mouse enable the user to reach easily any of the sub modes in the many modes. This software proved to be a reliable program for the hybrid environmental control system in the two greenhouse.

      • 오이 특징점 인식을 위한 영상처리 알고리즘 개발

        이원희,이대원,이용국 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

        An image processing algorithm was designed and constructed to recognize and measure the geometric shapes of a cucumber for a short real time using a computer vision system. The main components of the vision system included fluorescent lighting equipment as a light source, CCD (charge coupled device) camera, VGA (video graphics array), PC computer to grade a stationary cucumber. The algorithm was developed to recognize geometric features of a cucumber and also to reduce the processing time of them. Total image processing time was about 0.5 second, where only grabbing time was required more than 0.3 second. Geometric features include straight length, chord length, bending length, area, bending angle, maximum thickness and minimum thickness. Qualitative performance of the algorithm proved to be a reliable algorithm for geometric features of a cucumber.

      • 한국산 뱀독이 개구리 심장심실 근육 세포의 활동전압에 미치는 영향

        정해성,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.2

        Action potential of a frog ventricular cell was studied by conventional 3M KCI glass microelectrodes to investigate the effect of freeze-dried Korean snake venom. Following results were obtained; 1. Time to repolarize 50% of its action potential increased from 756±26mS to 796±17mS. 2. The notch which would be seen after the fast depolarization disappeared and the overshoot potential significantly decreased from 24.4±0.9㎷ to 17.8±2.3㎷. 3. Positive depolarization rate (dV/dt max) decreased from 22.8±0.8V/s to 18.8±0.8V/s. 4. Resting membrane potential was -95±3.0㎷ and showed little change. Above results would be interpreted that the Korean snake venom blocked the fast sodium channel and slow calcium channel simultaneously to reduce the contractility and activation of potassium channel which could result the prolongation of action potential duration.

      • ROBOT식 多目的 播種機 開發

        李鎔國,李大源,黃憲,吳永鎭 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

        Planting, transplanting, and harvesting are important processes for the successful production of farm products in Korea because those require the short term high labor intensity. In the automatic production system of the field or the green house, planting is the first process required to be automated considering subsequent operations such as transpalnting and harvesting. A proto type experimintal robotized seeder was designed and built to handle various sizes and shape of seeds. The developed seeder is automatically controlled with one chip micro-controller. System is composed of the transfer arm, pecker, and the equipments integrated with sensor such as hopper, feeder, counter, and shutter. System allows to control the number of seeds to be planted. Results of the developed experimental robotized seeder are as following : 1. For the adequate feeding and counting of seeds, components such as hopper, feeder, counter, and shutter were designed and built. 2. Kinematic motion of the transfer arm was analyzed and designed. 3. Picker was designed to dig and drop seeds into the soil by the open and close action of the bottom gate controlled by DC solenoid. Digging depth of the pecker was controlled by the transfer arm.

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