신생아 중증 세균성 감염에 대한 Augmentin(clavulanate potentiated amoxycillin)의 치료효과 및 안전성 연구

성인경,강진한,고대균,이경수 대한감염학회 1995 감염 Vol.27 No.1

Combination therapy of β-lactam antibiotics has been used widely to treat severe infections in neonates. The widespread use of β-lactam antibiotics has resulted in emergence of a broad variety of plasmid or chromosome mediated β-lactamases. Also, there is a great propensity for these enzymes to spread among various species of bacteria. Thus, the use of β-lactamase inhibitor antibiotics may be of great interest and has potential therapeutic value in severe neonatal infections. We tried to find out the efficacy and safety of clavulanate potentiated amoxycillin (Augmentin) in treatment of severe neonatal infections. Total thirty-five neonates with sepsis(26) or severe organ infections(9) were involved. Each 30mg/kg dose of Augmentin was injected at 12hr-or 8hr-or 6hr-intervals according to the age and weight of neonates. Clinically 77.1% of the cases were cured, and microbiologically 80.0% were eliminated. 88.9% of eighteen gram positive organisms(S. aureus, S. epidermidis, a -hemolytic strep., S. pneumoniae , E. faecalis) isolated from study group were susceptible to Augmentin. 47.0% of gram-negative organisms (K. pneumoniae, E.coli, P. mirabilis, S. marcescens, C. freundii) were suceptible. Thus, it was confirmed that Augmentin was very effective in ampicillin-or ceftriaxone-resistant gram positive cocci infections, but less effective in isolated K. pneumoniae and S.marcescens cases. The serum levels of amoxycillin in the neonates were maintined about 6㎍/ml until 1hr. at each injection. We observed that alkaline phosphatase level was elevated above 1,000IU/ℓ in three neonates and that skin rash developed after injection in one neonate. But, these side effects were mild and transient. These results suggest that Augmentin may be tolerable and effective in treating severe gram positive cocci infections in neonates.

N-벤조일-이미다졸 유도체의 가수분해반응

성대동,엄태섭,이종팔,박유미,박현석 東亞大學校 1992 東亞論叢 Vol.29 No.1

The pseudo first order rates for hydrolyses of N-benzoyl-(2,4,5-triphenyl)-imidazole and N-benzoyl-(4,5-diphenyl)-imidazole have been measured by means of spectrometry in over all pH range at 30˚C with the ionic strength of 0.5M KC1. In the case of N-benzoyl-(2,4,5-triphenyl)-imidazole, the general acid catalytic reaction was not observed, while the general base catalytic reaction showed in near the range of pH=7. In the case of N-benzoyl-(4,5-diphenyl)-imidazole, the general acid and base catalytic reaction showed at the regions of less than pH=4 and more than pH=10. The rate constants for pH independent reaction of N-benzoyl-(2,4,5,-triphenyl)-imidazole were shown a little bit faster than those of the corresponding to N-benzoyl-(4,5-diphenyl)-imidazole. The reaction mechanism of pH independent reaction was interpreted by means of thermodynamic parameters, and the isotope effect. The magni-tude of ΔH??, ΔS??, ??/?? values indicated that the bond breaking had been progressed more than bond formation in the transition state.

Au를 공침한 SnO₂후막센서의 가스 감응 특성

성정훈,강대원,이윤수,임준우,홍영호,이덕동 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

High selective and sensitive thick film type methane gas sensors were fabricated and their sensing characteristics for various hydrocarbon gases were investigated. Raw material, SnO_(2)/Au, prepared by coprecipitation was even grain size. SEM, TEM, BET and XRD analyses were carried out for investigation of surface morphology and crystalline structure. The thick film devices using the above materials exhibited high sensitivity to methane gas at the operating temperature of 400°C. The sensitivity of SnO_(2)/Au+Pd thick film devices to methane gas was higher than that to the other hydrocarbon gases such as iso-butane and propane. The optimal adding amounts of Au and Pd were 1wt.% and 3wt.%, respectively. The sensitivity, S, is defined as {(R_(A)-R_(G))/R_(A)}><100, which R_(A) and R_(G) are resistance in air and that in the gas ambient, respectively.

Oxametallaborane 및 Metallacarborane과 Phenyl Carbene 반응에 대한 반응성과 반응메카니즘

성대동,이용희,심경옥 동아대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.17 No.1

거대고리 뭉치 붕소화합물 중 산소원자를 포함하는 oxametallaborane과 탄소원자를 포함하는 metallacaborane을 이용하여 새로운 metallacarborane을 합성할 수 있는 방법을 알아보고 [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10]과 [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H12]를 합성한 후 이들에 phenyl diazomethane을 반응시켰다. [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10]과 phenyl diazomethane의 반응생성물은 예상과는 달리 cis-및 trans-stilbene과 trans, trans-benzaldehyde azine이었다. 카르벤의 이량화 반응은 [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10]의 촉매 작용에 의해 촉진 되었다. Oxametallaborane의 Pt-원자에 있는 d-오비탈이 카르벤의 삼중항 상태의 T1과 T2전자상태를 안정화 시켰다. [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H12]와 phenyl diazomethane의 반응생성 oxametallaborane의 결과와 유사하였고 동시에 미량의 C-카르벤 삽입 반응생성물이 생성되었다. Metallacaborane의 탄소 원자궤도함수인 SOMO와 phenyl diazomethane의 LUMO가 반응하여 분자궤도함수 에너지를 낮추는데 기여하였다. The reaction mechanism and a new synthetic method using oxametallaborane and metallacarborane compounds are investigated. [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10] and [9,9-(PMe2Ph)2-arachno-9,6-PtCB8H12] are synthesized then, reacted with phenyl diazomethane to find out a new synthetic method corresponding to the metallacarborane cage compound. Unexpectedly cis- and trans-stilbene and trans, trans-benzaldehyde azine are produced from the reaction of [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10] with phenyl diazomethane. [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H10] is operated as a kind of catalyst to yield dimerization product of carbene. The d-orbital in Pt-atom of oxametallaborane stabilizes the two triplet states of carbene, T1 and T2. A similar reaction products are produced from the reaction of [9,9-(PMe2Ph)2-arachno-9,6-PtOB8H12] with phenyl diazomethane and a trace amount of a new product is also produced such as C-carbene insertion reaction product simultaneously. The molecular orbital energy is lowered by the SOMO energy of metallacarborane and the LUMO energy of phenyl carbene.

R&D의 산업화와 지식집약형 기계산업 육성

성태현,정대철 경남대학교 경남지역문제연구원 2003 경남지역연구 Vol.8 No.-

Drosophila busckii의 韓國集團에서 있어서 同位酵素 多形現象

成耆昌,尙椿植,金彧,金大鎰 성균관대학교 기초과학연구소 1985 論文集 Vol.36 No.1

Genetic structure and variability were investigated in four Korean populations of Drosophila busckii. Eight allozyme loci were analysed by means of starch gel electrophoresis. The proportions of polymorphic loci and loci heterozygous per individual were estimated to be 0.25 and 0.051, respectively. The average genetic similarity between four populations was found to be S―=0.977. This value is similar to the other Drosophila populations reported. However, the gene variation of D. busckii in this study appeared to be the least variable among 28 Drosophila species reported. It is suggested that the low gene variation in D. busckii might be due to adaptation of this species to a narrow niche, but the possibility of occurrence of the random drift cannot be excluded.

Drosophila quinaria Species Group의 遺傳的 近緣關係

成耆昌,金正洙,金大鎰,金彧,金溶聲 성균관대학교 기초과학연구소 1985 論文集 Vol.36 No.2

The genetic relatedness among the Korean Drosophila quinaria species group, including D. nigromaculata, D. unispina, D. brachynephrose, and D. angularis, has been examined by using horizontal starch gel electrophoresis. The results showed that none of the ten isozyme patterns examined for these four species was identical. The average genetic similarity among the four species was found to be 0.440. The similarity among three species. D. unispina, D. brachynephrose, and D. angularis was 0.466. The relatedness was found to be closest between D. nigromaculata and D. unispina, showing 0.640. D. nigromaculata and D. angularis showed relatedness of 0.258. The results suggested that these four species appear to be genetically related at the level of sibling species. The percent polymorphism in D. brachynephrose was found to be 76.9%, showing significant genetic variations.

위 MALT 림프종에서 다발성 림프절 전이로 오인된 동시성 림프절 결핵 1예

김성은,김규종,김도현,송준영,문대성,장리라,유찬희,문원,박무인,박선자,김영옥 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

28세 남자가 내시경적 점막절제술을 통해 저등급 위MALT 림프종으로 진단되었다. 병기설정을 위해 18F-FDG PET 검사를 시행하였고 우측 쇄골상부, 우측상/하부 기관주위, AP window 부위에 강한 섭취가 관찰되어 전이성 림프절로 판단하였다. 그러나, 우측 쇄골상부 림프절에서 시행한 절제생검에서 만성육아종성염증소견이 관찰되었고 조직의 결핵 중합효소연쇄반응에서도 양성 반응을 보였다. 2개월간 항결핵제의 사용 후 시행한 추적 컴퓨터단층촬영 검사에서 림프절 크기의 감소를 보여 최종적으로 위 MALT 림프종에서 광범위 림프절 전이로 오인된 동시성 림프절 결핵으로 진단되었다. A 28-year-old man was diagnosed as gastric MALT lymphoma in diagnostic EMR (endoscopic mucosal resection). He subsequently underwent an 18F-FDG PET. 18F-FDG PET showed intense multifocal uptake in right supraclavicular, right upper and lower paratracheal, and AP window areas that was considered radiologically to represent widespread metastatic lymphadenopathy. Excisional biopsy of a supraclavicular lymph node, however, revealed chronic granulomatous lymphadenitis with a positive reaction of Tb-nested PCR. After antituberculosis treatment for 2 months, a follow-up CT scan showed complete resolution of the lesions. Intense 18F-FDG uptake could be due to an infectious process such as synchronous tuberculous lymphadenitis. Therefore, this could mimic a malignant condition such as lymphoma with extensive lymph node metastasis due to a false-positive finding, which may lead to a misdiagnosis.

초병렬시퀀싱 방법을 이용한 인간 별아교세포의 MicroRNA 프로파일링

김대용,안성민 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background : Astrocytes have multifunctional properties including structural support for nervous tissue and maintaining metabolic environment. Most primary brain tumors such as astrocytomas and glioblastoma originate from astrocytes. MicroRNAs, a class of non-protein-coding RNAs that are ~22 nucleotides in length, have been implicated in the regulation of various biological processes including apoptosis, animal development, physiological functions such as insulin secretion, and differentiation. Recently, miRNAs have been implicated in cancer as important regulators of tumour suppressor genes and proto-oncogenes. The purpose of this study was to provide the fundamental data for molecular-biological understanding and development of novel diagnosis and therapies of diseases involved with astrocytes by profiling microRNAs of human primary astrocytes. Methods : Human primary astrocytes were prepared from human fetal brains of 12-15 weeks gestation. Astrocytes were purified from other brain cells containing neurons, microglia and oligodendrocytes. Unlike hybridization-based methods, sequencing-based methods allow for direct discovery of novel microRNAs and detection of variations in mature microRNAs. In this study, massively parallel sequencing (MPS) was applied to microRNA profiling in primary human astrocytes. Results : In total, genomic alignments from 32378 sequence reads were annotated as miRNA, piRNA, snRNA, snoRNA, scRNA, scaRNA, tRNA, rRNA, mRNA or unknown based on their genomic position. Among the 721 known human miRNAs registered in miRBase, 260 miRNAs were recovered in primary human astrocytes libraries. In addition, 27 novel miRNA candidates wered identified from unknown clusters of mapped sequence reads. The total set of novel miRNA candidates comprises 27 unique miRNA sequences from introns (12 sequences), intergenic regions (8 sequences), 5’ UTR of protein-coding genes (2 sequences), and 3’ UTR of protein-coding genes (5 sequence). Conclusion : To the best of our knowledge, this presented work is the first to discribe a miRNA profiling in human primary astrocytes using massively parallel sequencing. Our study might be fundamental for the comprehension of the molecular basis of the pathogenesis of such an aggressive tumor as glioblastoma, and give new clues to develop targeted therapies against this still untreatable cancer. Additional studies are now obviously needed to experimentally identify the targets of modulated miRNAs and to correlate them with brain tumor oncogenesis.

삼배위 황화합물의 산촉매 가수분해 반응

성대동,이종팔,엄태섭 東亞大學校 大學院 1995 大學院論文集 Vol.20 No.-

The acid catalyzed hydrolysis of N-substituted sulfinamides have been studied in agueous solution of perchloric acid at 25℃ by spectrophotometrically. The pH-rate profile for N-phenylbenzene-sulfinamide(a); N-p-chlorophenybenzenesulfinamide(d), N-phenyl-p-chlorobe enesulfinamide(f) and N-phenyl-o-chlorobenzenesulfinamide (h) shows linearity, while those for N-p-methoxyphenylbenzenesulfin-amide(b), N-p-methylphenylbenzenesulfinamide(c) and N-phenyl-p-ethylbenzenesulfinamide(e) have a break at about pH 3. The break of pH-rate profile is ascribed to a change in the rate-determining step of the reaction involving a hypervalent intermediate.