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장현봉,김대중 목원대학교 건축도시연구센터 2003 建築·都市環境硏究 Vol.11 No.-
This paper investigates efficient policy programs for the traffic safety in Dae-Jeon Metropolitan area. For this, the characteristics of traffic environment in Metropolitan are reviewed and traffic accidents, traffic safety education and related policies are analyzed. Results are as follows; 1) The efficient co-operation scheme among traffic safety agencies and the specialization for traffic safety officers are needed. 2) Reinforcement of traffic safety education, especially for violation group. 3) Improvement of traffic safety facilities including the efficient operation of ITS.
두경부 종양세포주에 대한 Mithramycin A의 세포자멸사 유도성 및Cisplatin 항암작용 증가효과
이강대,손성환,박종욱,김영록,이봉주,김주연 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1
Background and Objectives : Cisplatin (CP) is a major chemotherapeutic agent for head and neck cancer. However, cancer cells can get drug resistance through recovering the DNA damage by CP. Mithramycin A (MA) can inhibit gene expression of multidrug resistance gene 1, c-myc or h-ras. The goal of this study is to evaluate the therapeutic effect of MA alone and combination of MA and CP on head and neck cancer cell lines. Materials and Methods : Two head and neck cancer cell lines (AMC-HN4, AMC-HN7) were treated with MA. The cellular proliferation, apoptosis and protein expression were measured with XTT assay, FACS analysis, and western blotting, respectively after MA treatment in cell lines. In order to know the combination effect of MA with CP, the cancer cell lines were treated with MA, CP, and MA/CP combination, and cellular proliferation, apoptosis and expression of apoptosis-associated proteins were measured. Results : MA inhibited the proliferation of head and neck cancer cells and induce dapoptosis. Increase of caspase activation, PLC-?1 fragmentation and percent apoptosis by MA treatment was blocked by z-VAD-fms, but not blocked by N-Acetyl Cystein. Combination of MA and CP significantly inhibited cancer cell growth and increased apoptosis compared to MA and CP alone, and it also suppressed the expression of anti-apoptotic proteins such as XIAP and Mcl-1. Conclusions : The present study suggests that MA alone andMA/CP combination chemotherapy could be a potential regiment for the control of the head and neck cancer.
RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
Oligodeoxyribonucleotides의 합성을 위한 유기염기의 보호기 도입
노봉오,조재흥,신대현 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1
In this study the starting materials, 2'-deoxyriboadenosine and 2'-deoxyribocytidine, were protected selectively as the tritylation of the 5'-hydroxyl group and the N-acylation of the amino group with 4,4'-dimethoxytrityl chloride and benzoyl chloride, in a oneflask procedure. Protection of the amino group and the 5'-hydroxyl group in 2'-deoxyguanosine can be performed directly by isobutyric anhydride and 4,4'-dimenthoxytrityl chloride pyridine to obtain 2-N-isobutyryl-5'-O-dimethoxytrityl-2'-deoxyguanosine in a one-flask procedure. The protected deoxyribonucleotides have been crharacterized on UV, IR and ^1H-NMR spectroscopy.
임대봉,박천익 대구대학교 경제경영연구소 2002 경제경영연구 Vol.1 No.2
우리는 과거에 고금리에만 너무 익숙해져 있어서인지 오늘날 금리는 거의 제로(0) 나아가서는 마이너스(-)의 금리수준에 직면해 있다. 본 논문에서는 회사채금리에 대해서 분석을 하였다. 우리는 그동안 저금리를 부러워만 했지만 이제 금리가 떨어지고 경기침체가 지속되자 과도한 저금리의 문제가 부각되고 있는 실정이다. 저금리의 영향으로 말미암아 소비와 경기침체는 물론이고 한계기업의 구조조정 지연 등 저금리의 부작용이 나타나고 있다. 그러면 이와 같은 저금리는 어떤 요인에 의해서 영향을 받게 되는지를 그랜져(Granger)인과관계검정, 충격반응함수, 그리고 마지막으로 예측오차의 분산분해 방법을 통해서 실증분석을 하였다. 분석결과에 의하면 회사채금리의 경우에 국내경제가 개방됨으로 인해 대외적인 요인인 주가, 환율, 미국의 회사채금리 수준 등에도 영향을 받는다는 결론을 얻을 수 있다. 저금리는 이자생활자에게 치명적인 영향을 미칠 뿐만 아니라 경제전체에도 저축률하락에 따른 투자재원 부족, 경상수지 악화 등 악영향을 미치게 된다. 그리고 경제주체들은 갑작스런 저금리 수준에 적응할 시간적 여유가 불충분했던 관계로 부동산 시장이 과열되는 현상을 초래할 수도 있다. 만약에 부동산 시장의 거품이 빠지면 가계부실과 금융기관의 부실로 이어질 수 있으므로 금리정책을 보다 신중하게 접근해야 할 것으로 보인다.
혈액투석중인 말기신부전 환자에서 Fludrocortisone acetate (FCA) 투여 후 혈청 칼륨 저하 효과
류봉관,강대웅,정지용,윤나라,신병철,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1
Background: Hyperkalemia is a commonly encountered problem in dialysis patients with end-stage renal disease. In this study we evaluated the effect of mineralocorticoid therapy (fludrocortisono acetate) on serum potassium level in the serum of hyperkalemic end-stage renal disease patients. Methods: Fourteen patients on hemodialysis receiving fludrocortisone acetate (FCA) 0.1 mg/day were observed for 2 months periods. Consecutive monthly biochemical profiles were compared for the druration of the pre-and post-treatment of FCA. Result: Fourteen patients with mean age (±SE) of 51.5 years (4 males and 10 females) and mean hemodialysis period of 51.9 months were studied. Mean serum potassium levels significantly fell (p<0.05) during the post-FCA period (5.2±0.66 mEq/L) compared with potassium levels during the pre-FCA (5.8±0.43 mEq/L) period. Pre-and post-FCA values were not different for sodium, chloride, protein, albumin, AST/ALT, glucose, blood nitrogen, creatinine, phosphate and calcium. Conclusions: FCA appears to decrease serum potassium value in patients with end-stage renal disease. These results suggested that FCA could be effective to treat hyperkalemia without any adverse effect in patients undergoing hemodialysis.
정봉용,주대헌,박주승,김명호 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.9
This paper reports an investigation of the characteristics of plasma duplex treated ductile cast irons during thermal fatigue tests on a thermal cycling rig. The thermal fatigue cracking behaviors and microstructure of untreated and plasma surface treated ductile cast irons have been investigated. Both ferrite and pearlite matrix ductile cast irons were evaluated. During the tests, 60 mm long cylinders with 20 mm diameter were subjected to 1,000 high frequency induction heating and water cooling cycles, lasting 7 and 5 sec, respectively. The thermal fatigue damage was evaluated by analyzing different crack dimensions and distribution by light microscopy. The results showed that the plasma surface treatment increased thermal fatigue cracking resistance. When thermal fatigue cracking occurs, the cracks always initiate at the surface of the specimen. The major path of crack propagation is generally along the ferrite grain boundaries. The hardened layer had an effect on the thermal fatigue property. In addition, the ductile cast iron with an pearlite matrix has a better resistance to thermal fatigue than that of ferrite matrix.
申奉戴 慶北專門大學 (영주경상전문대학) 1981 慶北專門大學 論文集 Vol.3 No.-
Compared with the advanced nations of world, however, bank card is not yet common phenomenon in our country. Thus, the proper understanding of the nature, development process, and function of the card is essential for businessmen and consumers. What is more important, the fact that credit card sales by card issuing company not to affliate with bank in our country has greatly increased over the past years is more urgent to understand the role of bank card. So objective of the thesis is to consider the historical growth of bank card plans, to explore the role of the card in consumer credit, to examine the contents of bank card business and to present the desirable directions for developing the cards in our country. In our country, credit card issuing companies have so many problems to improve in itself; the problems of financial companies, the organization of credit card center in case of departments, credit bureau or credit reporting service, improvement in collections, consumer credit protection, and security and fraud. But with the bank card, the consumer has the ability to purchase an infinite variety of goods and services from any one of many thousands of merchants of his choice. He need not carry many cards and checks and need not carry large amounts of cash, moreover, he need not worry about loss of cash as a result of theft, fire or other misfortune. He can obtain cash with his bank card from any participating bank, thus expanding the purchasing power of his card even bcyond the hundreds of thousands of retail establishments which honor the card itself. And also, with the bank card, the merchants will have the following merits. (1) The system requirements are minor compared to a store's own plan since there is no need for billing, maintaining customer files, mailing statement adjustments, etc. (2) A well-run basic plan is already a better budgeting tool for a consumer rather than a series of bills of from many different outlets. (3) In time, bank plan will expand the financial information services now included and make their plans even more attractive and useful. (4) The merchant has strong promoting backing from most bank plans can be expected to strengthen and increase their alliances. In conclusion, the most desirable way to develop consumer credit in our country is to introduce the bank card to have a support of the publish on the point of decreasing operation capitals from the merchant, operating an effective customer services and credit authorization, enlarging the inerchant-menibcrs and cardholders, encouraging savings deposits and aiming at checkless or cashless society.
뇌기저동맥단동맥류 소견을 보인 뇌혈관 조영술로 보이지 않는 중뇌-뇌교부위 혈관기형 : 증례보고 A Case Report
김봉수,한대희 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12
We present a case of mesencephalic-pontine vascular malformation which mimicks a basilar tip aneurysm. The patient showed headache. vomiting. confusion and nuchal rigidity after trauma. On the CT scan subarachnnoid hemorrhage in the prepontine and prepeduncular cistern and irregular high density in the mesencephalic-pontine area were observed. Cerebral angjography showed no definite aneurysm shadow but magnetic resonance angiogaphy revealed a round aneurysmal shadow in the basilar tip area. But the aneurysm was absent in the operative field and an entangled abnormal vascular mass was observed in the posterior basilar tip area. It was coagulated and wrapped with tissel. The patient died of rebleeding from the vascular malformation 11 days after the operation. Angiographically occult mesencephalic-pontine vascular malformation should be differentiated from the lesion mimicking basilar tip aneurysm and MR angiography has a limitation in the diagnosis of aneurysm when conventional angiography revealed no such a lesion.