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물-공기 계면에서 긴 사슬 양 말단 작용기 분자인 α,ω-13,16-Dimethyloctacosanedioate Dimethylester(C_30DME)의 거동에 관한 연구
이정근,손대원,이관수,송석호,김현명,정선호 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-
물-공기 계면에서 표면압력의 증가에 따른 C30DME 분자의 구조 변화를 살펴보았다. 표면압력(π)-표면적(A) 등온선을 통해 표면적이 줄어들 때 C30DME 분자가 선형으로부터 역 U 자형 구조로 변하는 것을 확인하였다. C30DME의 역 U 자형 구조의 형성을 확인하기 위하여 Surface Plasmon Resonance (SPR)와 ellipsometry를 이용하여 Langmuir-Blodgett (LB) film 의 두께를 측정하였고 이 두께는 C30DME 분자의 역 U 자형 구조의 높이와 일치함을 관찰하였다. 또한 Atomic Force Microscope (AFM)을 이용한 표면 거침도 측정의 결과는 선형으로부터 역 U자형 구조로의 구조적 변화가 불연속이라는 것을 보여주었다. We investigated the conformational change of C30DME molecule with an increase on surface pressure at the air-water interface. The surface pressure (π)-area (A) isotherm indicates that the structure of C30DME changed from linear to reverse U-shape as the surface area was compressed. In order to confirm the formation of reverse U-shape, we examined the thickness of Langmuir-Blodgett (LB) film by a surface plasmon resonance (SPR) and an ellipsometry. The result of surface roughness measurement using an atomic force microscope (AFM) showed that the conformational change from linear to reverse U-shape is discontinuous.
장상피화생과 Helicobactor pylori와의 상관 관계에 관한 연구
이복희,김대수,이현숙,진영주,윤세진,송형근,성노현,정현용,이동호 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2
In 1965 Lauren divided advanced gastric carcinoma into two main types-namely, "intestinal" and "diffuse" type carcinoma, which differ not only morphologically but also in their clinical and epidemiological characteristics. Moreover, a different histogenetic process has been postulated by many authors that intestinal type gastric carcinoma might arise from areas with intestinal metaplasia. Since Warren and Marshall reported the presence of Helicobacter pylori in the stomach in 1983, strong evidence has been obtained that there is a close relationship between Helicobacter pylori and chronic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal type gastric carcinoma. To study the relationship between intestinal metaplasia and Helicobacter pylori infection, we performed gastroscopic biopsies and CLO tests taken from patients with epigastric pain. Only 87 patients with macroscopically suspected antral gastritis were included. The prevalence of intestinal metaplasia in gastric antral biopsies increased from 33.3% in the age group<30 years to 63.6% in the age group 60 years. When all patients were divided into two age groups-namely, (i)<50 years(n=41) and (ii)≥ 50 years(n-=46), intestinal metaplasia was found significantly more often in patients ≥ 50 years. ((i) 39.2%, (ii) 54.3%, p<0.0001). The prevalence of Helicobacter pylori in gastric antral biopsies decreased from 66.7% in the age group<30 years to 62.5%, in the age group in the age group 50 to 59 years. When all patients were divided into the same two age groups-namely(i)<50 years and (ii)≥50 years, the prevalence of Helicobacter pylori did not differ between two groups in a statistically significant way. The mean age of intestinal metaplasia positive patients who were also Helicobacter pylori positive was 48±11.0(mean±S.D.)years, whereas the mean age of intestinal metaplasia positive patients who were Helicobacter pylori negative was 52±9.7(mean±S.D.)years. In conclusion. although our data do not prove a causal relationship between Helicobacter pylori infection and the histogenesis of intestinal metaplasia, we suggest that Helicobacter pylori plays an important role in the development of intestinal metaplasia in the gastric mucosa. Whether Helicobacter pylori has to be present at all times during this process or is only necessary as a 'trigger', needs further research.
세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구
이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1
Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.
Dae‑Geun Song,김은미,Jung Weon Lee 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.11
Cancer metastasis involves diverse cellular functionsvia bidirectional communications between intracellularand extracellular spaces. To achieve development of theanti-metastatic drugs, one needs to consider the efficacy andmode of action (MOA) of the drug candidates to block themetastatic potentials of cancerous cells. Rather than undertwo-dimensional environment, investigation of the metastaticpotentials under three-dimensional environment wouldbe much pharmaceutically beneficent, since it can mimic thein vivo tumor lesions in cancer patients, leading to allowanceof drug candidates analyzed in the 3D culture systems tolower failure rates during the anti-metastatic drug development. Here we have reviewed on the analyses of metastaticpotentials of certain cancer models in 3D culture systemssurrounded with extracellular matrix proteins, which couldbe supported by TM4SF5- and/or EMT-mediated actions. We particularly focused the initial events of the cancermetastasis, such as invasive outgrowth and disseminationfrom the cancer cell masses, spheroids, embedded in the 3Dgel culture systems. This review summarizes the significanceof tetraspanin TM4SF5 and Snail1 that are related to EMTin the metastatic potentials explored in the 3D gel systems.
Song, Dae-Geun,Kim, Yoon Suk,Jung, Byung Chul,Rhee, Ki-Jong,Pan, Cheol-Ho Humana Press 2013 Applied biochemistry and biotechnology Vol.171 No.7
<P>Parkin was originally identified as a protein associated with Parkinson's disease. Recently, numerous research studies have suggested that parkin acts as a tumor suppressor. In accordance with these studies, we previously reported that overexpression of parkin in HeLa cells induced growth inhibition. To elucidate possible mechanisms by which parkin may inhibit cell growth, HeLa cells were infected with adenoviruses expressing either the parkin gene or adenovirus alone for 72 h and a total proteomic analysis was performed using 2-D gel electrophoresis followed by LC-MS/MS. We identified three proteins whose expression changed between the two groups: the 40S ribosomal protein SA (RPSA) was downregulated in parkin virus-infected cells, and cytokeratins 8 and 18 exhibited an acid shift in pI value without a change in molecular weight, suggesting that these proteins became phosphorylated in parkin virus-infected cells. The changes in these three proteins were first observed at 60 h postinfection and were most dramatic at 72 h postinfection. Because upregulation of RPSA and dephosphorylation of cytokeratins 8/18 have been linked with tumor progression, these data suggest that parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18.</P>
Inhibitory effects of polyphenols isolated from Rhus verniciflua on Aldo-keto reductase family 1 B10
( Dae Geun Song ),( Joo Young Lee ),( Eun Ha Lee ),( Sang Hoon Jung ),( Chu Won Nho ),( Kwang Hyun Cha ),( Song Yi Koo ),( Cheol Ho Pan ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.4
Aldo-keto reductase family 1 B10 (AKR1B10) is a member of the NADPH-dependent aldo-keto reductase (AKR) superfamily, and has been considered to be a potential cancer therapeutic target. Total extract from the bark of Rhus verniciflua (Toxico-dendron vernicifluum (Stokes)) showed AKR1B10 inhibitory activity. To identify the active compounds from R. verniciflua responsible for AKR1B10 inhibition, nine compounds were isolated via bioactivity-guided isolation and tested for their effects against recombinant human AKR1B10 (rhAKR1B10). Results showed that butein, isolated from the ethyl acetate fraction, was most able to inhibit rhAKR1B10. The inhibitory rate of butein against rhAKR1B10 was 42.86% at 1 μM with an IC50 value of 1.47 μM, and enzyme kinetic analysis revealed its inhibition mode to be uncompetitive. [BMB reports 2010; 43(4): 268-272]
Amplification of Sulforaphane Content in Red Cabbage by Pressure and Temperature Treatments
( Song Yi Koo ),( Kwang Hyun Cha ),( Dae Geun Song ),( Dong Hwa Chung ),( Cheol Ho Pan ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Red cabbage, a Brassica vegetable, has glucoraphanin that is hydrolyzed by myrosinase to form sulforaphane, which has received attention due to its cancer chemopreventive activity. High hydrostatic pressure (HHP) treatment (100~400MPa) and subsequent incubation (20~80℃) were employed to amplify health beneficial sulforaphane content in red cabbage. The highest quantity of sulforaphane was 99.7 μmol/kg fresh weight from HHP treatment at 400MPa, followed by standing at 60℃. Cytotoxicity was determined to evaluate the side effect of HHP-treated red cabbage.