Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

대중음악 음원제작과정에서의 분쟁발생과그 개선점에 대한 고찰

강다혜 한국중재학회 2017 중재연구 Vol.27 No.2

본 연구는 대중음악 음원제작과정에서 일어나는 분쟁에 대한 해결점을 도출하는 것에 목적을 둔다. 우선 분쟁의 배경이 되는 음반형태의 시대적 흐름과 음원제작 과정을 살펴보았으며 그 과정 속에서의 문제점 및 해결방안을 제시하였다. 음원제작과정에서 발생할 수 있는 문제로는 창작자간의 표절 문제, 보조창작자에 대한 주창작자의 저작권권리침해 문제, 음향엔지니어의 권익 문제, 유통사 중심의 과점형성에 따른수직계열화 문제가 있다. 연구결과, 이들 문제를 해결하는데는 전문성, 비공개성, 우호성, 경제성, 신속성 등의 장점을 갖는 대체적 분쟁해결제도(ADR)가 적합하다는 결론에 이르렀다. 구체적인 ADR 활용방안으로는 한국저작권위원회 분쟁조정제도의 활성화, 표준계약서에 기재된 중재조항의 적극적 활용, 그리고 상호간 협상을 통한 인건비 및 음원 수익 재책정 등이있다. The purpose of this study is to find a solution to disputes in the process of producing digital music sources. At present, the center of the world music market including the domestic market has been completely transformed from the tangible musical record market to the intangible sound source market. Due to these environmental changes, the music production process becomes industrialized and specialized, causing conflicts of interest among the individuals in the process. First of all, this study examined changes in the music market which is the background of the dispute, identified the problems of the process and suggested solutions while summarizing the meaning and role of each process of producing a sound source that may arise during the sound production process. This study covers plagiarism between producers, copyright infringement of the creator against assistant creator caused by the industrialization and division of the production environment, issues related to the rights of sound engineers whose role and importance become bigger as acoustic technology develops and music genres become more diverse, and vertical hierarchy due to the formation of oligopoly by several distributors with huge capital. As a result of the study, it was concluded that Alternative Dispute Resolution (ADR) system is suitable for solving these problems. Specific methods of using ADR include activation of the dispute settlement system of the Korea Copyright Commission, active use of the arbitration clause specified in the standard contract, and recalculation of labor costs and earnings from copyright through mutual negotiations. This paper can be differentiated from previous studies in that it studied overall problems that might arise in the process of digital music source production and suggested ADR utilization as the solution.

결합조직형성 법랑모세포종의 방사선학적 소견 : 증례보고 3례

최다혜,허경회,문제운,이원진,허민석,이삼선,최순철,박관수 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Desmoplastic ameloblastoma is a rare histologic variant of ameloblastoma. It shows important differences in anatomic distribution, histologic appearance, and radiographic findings compared with the general type of ameloblastoma. It is histologically characterized by an abundance of densely collagenous stroma and radiographically a mixed radiolucent-radiopaque lesion. We present three cases of desmoplastic ameloblastoma. All the patients complained on buccal swelling with or without pain and the sites of occurrence were the anterior and the premolar region. Plain radiographs showed mixed radiopaque-radiolucent lesion with ill-defined or diffuse sclerotic margin and no external root resorption. Additionally, CT scans revealed buccal expansion and relatively well-defined margin of the lesions. The clinical and radiographic features of the presented cases were compared with those of the desmoplastic ameloblastoma in the previous literatures. (Korean J Oral Maxillofac Radiol 2006; 36 : 63-8)

직업훈련 프로그램이 장애인 직업준비도에 미치는 효과

이달엽,김다혜,이승욱 한국장애인재활협회 2004 재활복지 Vol.8 No.1

직업재활을 위해서는 무엇보다 장애인의 직업적 능력을 최대한으로 개발하고 적응시키기 위한 직업훈련이 필수적이며, 이러한 훈련을 통해 직업인으로서의 바탕이 되는 자질과 기능을 충분히 갖도록 해야 한다. 직업훈령에 있어서 단순한 직업적 기능의 훈련만으로는 효과를 거둘 수 없고 직업인으로서의 전인적 훈련이 필요하다. 따라서 장애인에게 적용되고 있는 직업훈련프로그램이 직업적응, 직업유지를 위해 가장 바탕이 되는 직업준비도에 어느 정도 영향을 미치는 효과가 있는지를 파악하여 직업훈련서비스 제공에 있어서 개선을 위한 기초자료로 활용하고자 하였다. 구체적인 연구문제로 직업훈련프로그램이 장애인의 직업태도, 대인관계 및 사회기술, 직업생활 유지에 필요한 직업에 대한 인식과 직업적응에 미치는 효과와 장애인 직업준비도 요인간의 영향에 대해서 알아보았다. 본 연구에서는 WPP, VALPAR 17, 진로태도성숙도, 자기효능감 측정 도구를 사용하여 대구직업전문학교 입학생 16명을 대상으로 4-5개월 동안 직업훈련프로그램 적용 전과 적용 이후 직업인성, 대인관계 및 사회기술, 직업인식, 자기효능감을 측정하여 직업준비도의 변화정도를 파악하였다. 직업준비도에 미치는 효과를 알아보기 위해 실시한 연구를 통해 나타난 결과들은 다음과 같다. 첫째, 직업준비도 요인 변화에 있어서 직업에서 요구하는 일에 적응하는데 중요한 요인이 되는 직업인성은 직업훈련프로그램 적용 이후 향상되었고, 직업준비를 위한직업인성 형성에 효과가 있는 것으로 나타났다. 둘째, 직업훈련프로그램 적용이 장애유형에 따른 직업준비도에 미치는 영향은 직업인성과 직업인식, 자기효능감에서 장애유형에 따라 효과의 차이가 발생하였다. 셋째, 직업준비도 요인간의 상관관계는 직업인성이 높으면 자기효능감도 높아지며, 또한 자기효능감이 높을수록 직업인식이 높아지는 것으로 나타났다. 넷째, 직업준비도 요인에 대한 독립변수들의 상대적 기여도를 알아보기 위해 실시한 회귀분석 결과에서 대상자들의 나이와 장애유형, 대인관계와 사회기술이 직업인성에 큰 영향을 미치는 것으로 나타났으며, 대인관계 및 사회기술은 아니와 직업인성이 큰 영향을 미치는 것으로 나타났다. 결론적으로 직업생활 유지에 있어서 직업적응을 위한 작업준비도를 높이기 위해서는 직업인성을 향상시킬 수 있는 직업훈련프로그램이 필요하며, 이러한 직업인성을 향상시킬 수 있는 방법은 대인관계 및 사회기술 영역에 비중을 둘 수 있는 직업훈련프로그램이 필요하다. 그리고 직업훈련프로그램의 직업유지나 직업적응에 대한 효과성 조사와 훈련교사들에 대한 교육도 지속적으로 이루어져야 하며, 이러한 것들이 프로그램구성이나 교과과정 작성 시 반영하는 연구설계가 필요하다. It is essential for rehabilitating persons with disabilities, to develop employment skills. To maximize vocational potential and abilities of persons with disabilities, it is required for them to obtain work attitudes and skills. However, the work skills training alone may not be enough for persons with severe disabilities, requiring them t o have work preparation services. Therefore, this study was aimed to understand whether the vocational training program provided by Dae-Gu Metropolitan Training Institute for Persons with Disabilities had impact on the improvement of both work skills and job readiness skills for them. Data gathered included the following areas. The effect of the disabled's vocational behaviour. the personal relations, the social skills, the vocational consciousness and the job readiness areas. The WPP, VALPAR 17, Course Attitude Maturity, Self-Efficacy Feeling Measurement Tool were utilized for the data gathering purposes. For vocational Rehabilitation, first of all, it is essential for disabled trainees to develop and adapt through individualized service provisors. They are required to have enough appropriate attitudes and skills which basic to average workers. For the vocational training, a simple vocational skills training alone may not be enough. Therefore, this study was aims to figure out whether the vocational training program which have applied to the trainees in Dae-Gu Vocational Training Institute (DGVTI) was effective or not. This training program was composed of the work adaptation training and job readiness training. Initial study questions posed are as follows. Whether this training program had effect on the research participants' vocational behaviours, personal relationship skills, social skills, the vocational consciousness and the job readiness skills. The current researcher utilized the WPP, VALPAR 17, Course Attitude Maturity. Self-Efficacy Feeling Measurement Tool. They measure types of vocational personality. personal relations and social skills, vocational and self-efficacy feeling in comparison with before and after the application of training program. The 16 research participants had exposed to 4-5 months in Dae-Gu Vocational Training Institute(DGVTI), operated by Korea Employment Promotion Agency for the Disabled (KEPAD). Statistical analyses focused on the means, standard deviations, and frequencies. Futher analyses included t-tests, correlation Analyses, regression analyses. The results of this study can be summarized as follows. First, in the job readiness skills area, after training, vocational personality (which is the important factor adapting to assignment demanding occupation) is improved, and reveals that the formation of vocational personality for job readiness and, in the vocational recognition and self-efficacy feeling are positively changed. Second. job readiness skills had effect to improve research participants' vocational adaptation skills, vocational recognition and self-efficacy feeling. Third. "correlation between job readiness factors" appeared that if scores of the vocational personality is high, then the self-efficacy feeling also rises. When the self-efficacy scores are high, the vocational recognition rises. Fourth, the results of regression analyses indicated that the age and the types of disabilities of the research participants had influenced on the occupational personality. Also, research participants who showed good personal relation skills and social skills had good job readiness. It is conclusive that the method of improving occupational personality would influence on personal relations and social skills. In conclusion, it is necessary for the rehabilitation practitioners to provide skills and interpersonal skills with their clients and trainees. The implication of this study is that there is a strong need to improve quality of vocational training programs.

Maslow욕구 단계에 따른 속옷 추구혜택과 소비행동에 대한 질적 연구

봉규희;안혜진;이다미 한양대학교 2012 韓國 生活 科學 硏究 Vol.32 No.1

The purpose of this research was to examine the influence of basic needs on benefit segmentation and consumption behavior of underwear market. Maslow’s hierarchy of needs was the theoretical grounding of this study. For empirical investigation focus group interviews were conducted. A total of 12 women participated. Analysis of the qualitative data indicated that underwear consumption behavior was originated from basic need stages, also it is evidenced that the basic needs stage operated simultaneously, not progressively. There was a correlation between basic needs and benefits sought. At the same time various mediators had effect on underwear consumption behavior, and the mediators brought about several different consumption behaviors. It showed that a specific benefit sought was formed by each consumer’s psychological background, not by only single basic need.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

The applicability assessment of non-invasive bio-monitoring method for PFCs in place of existing invasive bio-monitoring method

Da-Hye Kim(김다혜),Jong-Hyeon Lee(이종현),Jeong-Eun Oh(오정은) 환경독성보건학회 2015 한국독성학회 심포지움 및 학술발표회 Vol.2015 No.10

Predictive Factors on Blood Donation Intention in Middle Aged Public Officials Base on the Theory of Planned Behavior

Da jung Lee,Hye-Kyung Lee,Hye-Sun Jeong,Hyun-Kyung Kim,Suyon Baek 한국성인간호학회 2021 성인간호학회 학술대회 Vol.2021 No.8

Development of BrOR based Marker for Selection of Carotenoid-enriched Chinese Cabbage Brassica rapa

Da-Hye Kim,GyuMin Park,Sun-Hyung Lim 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.5

Genetic Association Between the Native Color(Red, Red-line) and The Ogol in Korean Native Chickens Using RAPD Marker

Hye-Mi Park,Da-Hye Shin,Sang-Hwan Kim,Chan-Seong Jeong 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

This study was conducted to evaluate the genetic distances and specific DNA makers by the randomly amplified polymorphic DNA (RAPD)-PCR method for the Korean native chickens(red plumage, red-line plumage, Ogol) and white leghorn. Genomic DNA was extracted from plumage from chickens after they were slaughtered. The extracted DNA was observed by nano-spectrometer. RAPD analysis was performed using 13 different primers. Statistical analysis was made for the estimation of the genetic distance among the chicken’s and the cluster tree was drawn by using MEGA 5.05 software. Genetic relations among them were determined by RAPD analysis. The polymorphic bands were observed 72% and the rest of 28% was monomorpic. The largest genetic distance (2.266) was found between the native chickens(red, red-line) and the ogol chickens by UPGMAP method and the closest distance was observed between the ogol in korean chickens as expected. The highest genetic distance between them was estimated 2.266 and in the dendrogram analysis, among I and II within cluster II, most of the ogol chickens were in IIB, indicating the expression of the ogol color could be due to original and the ancestral genetic crossing. Thus, this genetic distance can be useful as the differential genomic information in the normal(red plumage, red-line plumage) and ogol of korean native chickens. This work was supported by a grant from the “Livestock Preservation of Genetic Resources", Rural Development Administration, Republic of Korea.