Proteomic Profiling of Serum from Stage I Lung Squamous Cell Carcinoma Patients

Li, Xin-Ju,Wu, Qi-Fei,He, Da-Lin,Fu, Jun-Ke,Jin, Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Objectives: This study employed proteomic profiling to identify specific tumor markers that might improve early diagnosis of lung squamous cell carcinoma. Methods: Serum samples were isolated from 30 patients with stage I lung squamous cell carcinoma and 30 age-and gender-matched healthy controls, and proteomic profiles were obtained by matrix-assisted laser desorption ionization time of flight mass spectrometry. Results: Three highly expressed potential tumor markers were identified in the sera of stage I lung squamous cell carcinoma patients, with molecular weights of 3261.69, 3192.07, and 2556.92 Da. One protein peak with molecular weight 3261.69 Da was chosen as the candidate biomarker and identified as a fibrinogen alpha chain through a search of the IPI, NCBI or SWISS-PROT protein databases. Conclusion: As a potential tumor biomarker, fibrinogen alpha chain may be applicable for the early diagnosis and prognosis of lung squamous cell carcinoma patients.

Enhancement of Virus-induced Gene Silencing in Tomato by Low Temperature and Low Humidity

Da-Qi Fu,Ben-Zhong Zhu,Hong-Liang Zhu,Hong-Xing Zhang,Yuan-Hong Xie,Wei-Bo Jiang,Xiao-Dan Zhao,Yun-Bo Luo 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1

Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDSinoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature (15°C) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.

Excess trehalose and glucose affects chitin metabolism in brown planthopper (Nilaparvata lugens)

Qi-Da Shen,Meng-Meng Yang,Guo-Qiang Xie,Hui-JuanWang,Lu Zhang,Ling-Yu Qiu,Shi-GuiWang,Bin Tang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Trehalose is a non-reducing disaccharide that is hydrolyzed by trehalase to yield two glucose molecules. Trehalase is the first enzyme involved in the chitin biosynthesis pathway, and it plays a pivotal role in insect growth and molting. In this study, Nilaparvata lugens, an economically important rice pest in Southeast Asia, was injectedwith trehalose or glucose to investigate their effects on chitin metabolism. Excess trehalose and glucose significantly increased the rate of deformity (molting and/or wing deformities) and mortality in N. lugens. Trehalose, glycogen, and glucose contents were also significantly decreased in N. lugens treated with trehalose or glucose. Chitin content and the expression of NlHK, NlUAP, NlG6PI1, NlGFAT, NlGNPNA, NlPGM1, NlPGM2, NlCHS1, NlCHS1a, NlCHS1b, NlCht3, NlCht4, NlCht6, and NlCht7 were significantly decreased, whereas the expression of NlCht2, NlIDGF, and NlENGase was significantly increased in treated insects. Furthermore, a significant increase in the expression of NlTRE1-1, NlTRE2, and NlTPS1 and a decrease, in the expression of NlTPS2 were observed. Results of this study suggested that excess trehalose and glucose could affect chitin metabolism by regulating the expression of pivotal genes to decrease the chitin content, resulting in the inability of N. lugens to complete its molting process.

Applying Multifractal Spectrum Theory to Fingerprint Features Recognition

Hai Ming Ni,Da Wei Qi,Hongbo Mu 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1

Fingerprint features recognition which can be used to distinguish between individuals is an intriguing study with many potential applications. In this paper, a new method for fingerprint recognition based on multifractal spectrum theory was proposed. The recognition process can be divided into the following main steps: (1) Extracting the core point in fingerprint; (2) Fragmenting the fingerprint image to get a subimage with fixed size; (3) Thinning the fingerprint image by using an improved OPTA algorithm; (4) Segmenting the curves in fingerprint image into digital straight segments with normalized straight length threshold; (5) Selecting the appropriate dividing scale to segment the processed fingerprint image; (6) Calculating and analyzing the multifractal spectrum curve - f (a) ; (7) Fitting curve equation and extracting the characteristic parameters of a - f(a); (8) Finally, the parameters matching and fingerprint feature recognition. A large number of experimental results show that our method is effective.

FOUR LOGARITHMICALLY COMPLETELY MONOTONIC FUNCTIONS INVOLVING GAMMA FUNCTION

Qi, Feng,Niu, Da-Wei,Cao, Jian,Chen, Shou-Xin Korean Mathematical Society 2008 대한수학회지 Vol.45 No.2

In this paper, two classes of functions, involving a parameter and the classical Euler gamma function, and two functions, involving the classical Euler gamma function, are verified to be logarithmically completely monotonic in $(-\frac{1}{2},\infty)$ or $(0,\infty)$; some inequalities involving the classical Euler gamma function are deduced and compared with those originating from certain problems of traffic flow, due to J. Wendel and A. Laforgia, and relating to the well known Stirling's formula.

Image-processing Based Adaptive Human-computer Interaction System

Qi-Jie Zhao,Da-Wei Tu,Zhi-Hua Huang 동국대학교 정보융합기술원 2007 International Journal of Assistive Robotics and Sy Vol.8 No.3

Comparison of the Efficacy and Safety of EFGR Tyrosine Kinase Inhibitor Monotherapy with Standard Second-line Chemotherapy in Previously Treated Advanced Non-small-cell Lung Cancer: a Systematic Review and Meta-analysis

Qi, Wei-Xiang,Shen, Zan,Lin, Feng,Sun, Yuan-Jue,Min, Da-Liu,Tang, Li-Na,He, Ai-Na,Yao, Yang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Purpose: To compare the efficacy and safety of epidermal growth factor receptor tyrosine kinase inhibitormonotherapy (EFGR-TKIs: gefitinib or erlotinib) with standard second-line chemotherapy (single agent docetaxel or pemetrexed) in previously treated advanced non-small-cell lung cancer (NSCLC). Methods: We systematically searched for randomized clinical trials that compared EGFR-TKI monotherapy with standard second-line chemotherapy in previously treated advanced NSCLC. The end points were overall survival (OS), progression-free survival (PFS), overall response rate (ORR), 1-year survival rate (1-year SR) and grade 3 or 4 toxicities. The pooled hazard ratio (HR) or risk ratio (RR), with their corresponding 95% confidence intervals (CI) were calculated employing fixed- or random-effects models depending on the heterogeneity of the included trials. Results: Eight randomized controlled trials (totally 3218 patients) were eligible. Our meta-analysis results showed that EGFR-TKIs were comparable to standard second-line chemotherapy for advanced NSCLC in terms of overall survival (HR 1.00, 95%CI 0.92-1.10; p=0.943), progression-free survival (HR 0.90, 95%CI 0.75-1.08, P=0.258) and 1-year-survival rate (RR 0.97, 95%CI 0.87-1.08, P=0.619), and the overall response rate was higher in patients who receiving EGFR-TKIs(RR 1.50, 95%CI 1.22-1.83, P=0.000). Sub-group analysis demonstrated that EGFR-TKI monotherapy significantly improved PFS (HR 0.73, 95%CI: 0.55-0.97, p=0.03) and ORR (RR 1.96, 95%CI: 1.46-2.63, p=0.000) in East Asian patients, but it did not translate into increase in OS and 1-year SR. Furthermore, there were fewer incidences of grade 3 or 4 neutropenia, febrile neutropenia and neutrotoxicity in EGFR-TKI monotherapy group, excluding grade 3 or 4 rash. Conclusion: Both interventions had comparable efficacy as second-line treatments for patients with advanced NSCLC, and EGFR-TKI monotherapy was associated with less toxicity and better tolerability. Moreover, our data also demonstrated that EGFR-TKImonotherapy tended to be more effective in East Asian patients in terms of PFS and ORR compared with standard second-line chemotherapy. These results should help inform decisions about patient management and design of future trials.

Single-phased silicate-hosted phosphor with 660 nm-featured band emission for biological light-emitting diodes

Qi-fei Lu,Jian Li,Da-jian Wang 한국물리학회 2013 Current Applied Physics Vol.13 No.7

A single-phased (Ba,Sr)3MgSi2O8:Eu2þ, Mn2þ phosphor with 660 nm-featured dual band-emission is investigated upon optimizing composition to simulate the artificial photosynthetic action spectrum (PAS)for near-ultraviolet (NUV) biological light-emitting diodes (bio-LEDs). A specific composition range in Ba eSr binary solid solution of (Ba,Sr)3MgSi2O8 is found to be capable of obtaining single-phased host in the absence of an easily formed orthosilicate impurity, leading to a 660 nm-featured red band emission of Mn2+ induced by an efficient energy transfer from a co-doped blue-emitting Eu2+ sensitizer. This dual broad band emission phosphor has a 72 nm full width at half maximum (FWHM) for red band that covers fairly well to the absorption spectrum of chlorophyll and PAS for most plants, enabling a flexible option in the application of bio-illumination for artificial photosynthesis.

Image-processing Based Adaptive Human-computer Interaction System

Qi-Jie Zhao,Da-Wei Tu,Zhi-Hua Huang 한국과학기술원 인간친화 복지 로봇 시스템 연구센터 2007 International Journal of Assistive Robotics and Me Vol.8 No.3

  In this paper, we have taken into account some impact factors on Infrared TVbased eye gaze input system, and proposed some solutions to these problems. In order to improve the adaptation of eye-gaze input modality to large variation of environment light and its lighteness, a set of image capturing equipment and image thresholding model has been put forward. Furthermore, some control strategies and methods have been proposed to decrease the influences on the eye gaze system caused by users head movement. At last, some experiments have been done to verify the feasibility of the strategies and methods. The results have shown that the system permits user’s head freely move in certain conditions. Moreover, higher accuracy can be gotten for eye gaze input.

Prospective UNCITRAL Technical Assistance for Asian Countries in International Arbitration

( Qi Sheng He ),( Da Yong Zhou ) 고려대학교 법학연구원 2011 The Asian Business Lawyer Vol.7 No.-

The United Nations Commission on International Trade Law (UNCITRAL) is a core legal body established by the United Nations, which takes the mandate to harmonize the international and regional trade law, and to create an environmentfacilitating international trade and commercial transactions. In the field of international commercial arbitration, UNCITRAL conducts cooperation programs and provides technical assistance to facilitate its mission. In Asia, facing a rapid regional economic boost but divergent legal systems, improving the professional quality and credibility of regional arbitral institutes shall meet the continual increased regional need for commercial arbitrations and guarantee the accurate application of international law. This article introduces the function of UNCITRAL, gives a brief analysis of the demand for improving international commercial arbitration in Asia, and suggests that UNCITRAL as an appropriateorganization may prospectively contribute to promote the competences of regional arbitral institutes and arbitral participants by means of providing active technical assistance and cooperative projects. Two tentative proposals are put forward in this article from a practical perspective.