Identification of Biomarkers for Diagnosis of Gastric Cancer by Bioinformatics

Wang, Da-Guang,Chen, Guang,Wen, Xiao-Yu,Wang, Dan,Cheng, Zhi-Hua,Sun, Si-Qiao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Background: We aimed to discover potential gene biomarkers for gastric cancer (GC) diagnosis. Materials and Methods: Genechips of 10 GC tissues and 10 gastric mucosa (GM, para-carcinoma tissue, normal control) tissues were generated using an exon array of Affymetrix containing 30,000 genes. The differentially expressed genes (DEGs) between GC tissues and normal control were identified by the Limma package and analyzed by hierarchical clustering analysis. Gene ontology (GO) and pathway enrichment analyses were performed for investigating the functions of DEGs. Receiver operating characteristics (ROC) analysis was performed to measure the effects of biomarker candidates for diagnosis of GC. Results: Totals of 896 up-regulated and 60 down-regulated DEGs were identified to be differentially expressed between GC samples and normal control. Hierarchical clustering analysis showed that DEGs were highly differentially expressed and most DEGs were up-regulated. The most significantly enriched GO-BP term was revealed to be mitotic cell cycle and the most significantly enriched pathway was cell cycle. The intersection analysis showed that most significant DEGs were cyclin B1 (CCNB1) and cyclin B2 (CCNB2). The sensitivities and specificities of CCNB1 and CCNB2 were both high (p<0.0001). Areas under the ROC curve for CCNB1 and CCNB2 were both greater than 0.9 (p<0.0001). Conclusions: CCNB1 and CCNB2, which were involved in cell cycle, played significant roles in the progression and development of GC and these genes may be potential biomarkers for diagnosis and prognosis of GC.

A Parametric Method of Linear Functional Observers for Linear Timevarying Systems

Da-Ke Gu,Long-Wen Liu,Guang-Ren Duan 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.3

This paper considers the design of a Luenberger observer to estimate the linear multiple states functionalfor linear time-varying (LTV) systems. Based on the solutions to a type of full actuated homogeneous generalizedSylvester matrix equations and the conditions for the existence of observers for LTV systems, general parametricsolutions to Luenberger functional observers are established. With the proposed approach, the functional observerscan be achieved at desired convergence rate of the observation error, and also without any particular transformationfor LTV systems. Finally, a numerical example shows the feasibility and effectiveness of the proposed parametricdesign method for the functional observers in LTV systems.

Static Output Feedback for Discrete-time Switched Linear Systems under Arbitrary Switching

Guang-Hong Yang,Da-Wei Ding 제어·로봇·시스템학회 2010 International Journal of Control, Automation, and Vol.8 No.2

This paper considers the problem of static output feedback (SOF) control for discrete-time switched linear systems under arbitrary switching. By the aid of switched quadratic Lyapunov functions combined with Finsler’s lemma, new sufficient conditions for SOF stabilization are obtained which guarantee a r-performance of the closed-loop switched systems subject to input disturbances. The proposed method can work successfully in situations where the existing ones fail. Three examples are given to illustrate its effectiveness.

Preparation and properties of ZrB2-YAG-Al2O3 ceramics

Jie-Guang Song,Gang-Chang Ji,Shi-Bin Li,Yang-Liang Li,Da-Ming Du,Lian-Meng Zhang,Yin-Yan Ju 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.4

ZrB2, YAG and Al2O3 are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To achieve better properties in ZrB2 ceramics, high-density ZrB2-YAG-Al2O3 ceramics were prepared. Below 1000℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is less than that of ZrB2-YAG ceramics. From 1000℃ to 1600℃, the second largest shrinkage occurs. Above 1600℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is more than that of ZrB2-YAG ceramics. The fracture toughness of sintered ceramics from coated raw materials is higher than that of sintered ceramics with mixed raw materials with the same phases and phase content, the fracture toughness of ZrB2-YAG-Al2O3 ceramics is higher than that of ZrB2-YAG ceramics with the same raw materials. The weight gain of all types of ceramics is increased with all increase in the oxidation temperature, the weight gain of ceramics is reduced with all increase in the YAG-Al2O3 content and Al2O3 proportion, especially above 1500℃. ZrB2, YAG and Al2O3 are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To achieve better properties in ZrB2 ceramics, high-density ZrB2-YAG-Al2O3 ceramics were prepared. Below 1000℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is less than that of ZrB2-YAG ceramics. From 1000℃ to 1600℃, the second largest shrinkage occurs. Above 1600℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is more than that of ZrB2-YAG ceramics. The fracture toughness of sintered ceramics from coated raw materials is higher than that of sintered ceramics with mixed raw materials with the same phases and phase content, the fracture toughness of ZrB2-YAG-Al2O3 ceramics is higher than that of ZrB2-YAG ceramics with the same raw materials. The weight gain of all types of ceramics is increased with all increase in the oxidation temperature, the weight gain of ceramics is reduced with all increase in the YAG-Al2O3 content and Al2O3 proportion, especially above 1500℃.

Genetic Variants at 6p21.1 and 7p15.3 Identified by GWASs of Multiple Cancers and Ovarian Cancer Risk: a Case-control Study in Han Chinese Women

Li, Da-Ke,Han, Jing,Liu, Ji-Bin,Jin, Guang-Fu,Qu, Jun-Wei,Zhu, Meng,Wang, Yan-Ru,Jiang, Jie,Ma, Hong-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

A recent study summarized several published genome-wide association studies (GWASs) of cancer and reported two pleiotropic loci at 6p21.1 and 7p15.3 contributing to multiple cancers including lung cancer, noncardia gastric cancer (NCGC), and esophageal squamous-cell carcinoma (ESCC) in Han Chinese. However, it is not known whether such genetic variants have similar effects on the risk of gynecologic cancers, such as ovarian cancer. Hence, we explored associations between genetic variants in 6p21.1 and 7p15.3 and ovarian cancer risk in Han Chinese women. We performed an independent case-control study by genotyping the two loci (rs2494938 A > G at 6p21.1 and rs2285947 A > G at 7p15.3) in a total of 377 ovarian cancer cases and 1,034 cancer-free controls using TaqMan allelic discrimination assay. We found that rs2285947 at 7p15.3 was significantly associated with risk of ovarian cancer with per allele odds ratio (OR) of 1.33 [95% confidence interval (CI): 1.08-1.64, P=0.008]. However, no significant association was observed between rs2494938 and ovarian cancer risk. Our results showed that rs2285947 at 7p15.3 may also contribute to the development of ovarian cancer in Han Chinese women, further suggesting pleiotropy of 7p15.3 in multiple cancers.

A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer

Ji-Guang Han,Yong-Dong Jiang,Chun-Hui Zhang,Yan-Mei Yang,Da Pang,Yan-Ni Song,Guo-Qiang Zhang 대한외과학회 2017 Annals of Surgical Treatment and Research(ASRT) Vol.92 No.2

Purpose: Insufficient sensitivity and specificity prevent the use of most existing biomarkers for early detection of breast cancer. Recently, it was reported that serum microRNAs (miRNAs) may be potential biomarkers in many cancer diseases. In this study, we investigated whether serum levels of 5 miRNAs including miR-21, miR-125b, miR-145, miR-155, and miR-365 could discriminate breast cancer patients and healthy controls. Methods: Serum levels of miRNAs were measured by using quantitative real-time polymerase chain reaction in 99 breast cancer patients and 21 healthy controls. The abundance change of serum miRNAs were also evaluated following surgical resection in 20 breast cancer patients. Receiver operating characteristic (ROC) curve analysis was performed to assess the sensitivity and specificity of miRNAs as diagnostic biomarkers. Results: Serum levels of miR-21 and miR-155 was significantly higher, while miR-365 was significantly lower in breast cancer as compared with healthy controls. The serum levels of miR-21 and miR-155 significantly decreased following surgical resection. Additionally, the serum level of miR-155 at stages I and II was significantly higher compared to stage III. The serum miR-145 level was remarkably higher in progesterone receptor (PR)-positive patients than PR-negative. The positivity of miR-21, miR-155, and miR-365 was high compared to CA 153 and CEA in breast cancer. ROC curve analyses of a combination of miR-21, miR-155, and miR-365 yielded much higher area under curve and enhanced sensitivity and specificity in comparison to each miRNA alone. Conclusion: The combination of serum miR-21/miR-155/miR-365 may potentially serve as a sensitive and specific biomarker that enables differentiation of breast cancer from healthy controls.

Effect of the sintering technology on the properties of fired brick from quartz sands

Jie-Guang Song,Fang Wang,Xiao-Bo Bai,Da-Ming Du,Yin-Yan Ju,Ming-Han Xu,Gang-Chang Ji 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.4

River sands as low grade natural raw materials are used to prepare the fired brick to replace clay fired bricks, which aims to save land resources and use low grade natural resources to realize high cost performance. In this paper, the effect of sintering technology of bricks on their performance is discussed. The compressive strength of fired bricks is first increased and then decreased with an increase in the sintering temperature, the compressive strength of bricks prepared by the isostatic compaction shaping is higher than that of bricks prepared by the plastic shaping under the same conditions. The compressive strength of bricks reaches 28 MPa when sintered at 1150 oC by using a 20MPa isostatic compaction shaping method. The compressive strength of bricks is first increased and then decreased by prolonging the holding time, the compressive strength of bricks reaches 28.9MPa when sintered at 1150 oC for 2 h.

Oxidation behavior of YAG-ZrB2 ceramics prepared by spark plasma sintering

Jie-Guang Song,Da-Ming Du,Yin-Yan Ju,Yang-Liang Li,Shi-Bin Li,Gang-Chang Ji 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4

ZrB2 and YAG are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To make ZrB2 ceramics have better oxidation resistance, high-density YAG-ZrB2 ceramics were prepared. The oxidation behavior of YAG-ZrB2 ceramics is investigated at different temperatures. The results indicate that the enhanced weight change differs according to the temperature range, slightly in the temperature 900-1000oC, sharply from 1100-1300 and smoothly above 1300oC. The weight change rate of YAG-ZrB2 ceramics is increased by prolonging the oxidation time, but the weight change rate of YAG-ZrB2 ceramics is gradually reduced. The oxidation layer is porous as compared to the YAG-ZrB2 ceramics layer.

The effect of the oxidation conditions on the surface microdomains of ZrB2-YAG ultra-high temperature ceramics

Jie-Guang Song,Min-Han Xu,Da-Ming Du,Fang Wang,Shi-Bin Li,Gang-Chang Ji 한양대학교 세라믹연구소 2013 Journal of Ceramic Processing Research Vol.14 No.4

Zirconium diboride is widely applied because of some excellent properties. The effect of the oxidation temperature on the surface microdomain of ZrB2-YAG multi-phase ceramics was studied, it helps to improve the performance of ultra-hightemperature ceramics. The results show the oxidation layer thickness is increased with an increase in the oxidation temperature, the oxidation layer thickness is decreased by increasing the density of multi-phase ceramics at the same oxidation temperature. The ceramic surface shows the (m)ZrO2, YAG, B2O3 and ZrB2 phases below the the oxidation temperature at 1300 ο C, but the ceramic surface do not show the ZrB2 phase above the oxidation temperature at 1300 ο C that is to say, the ZrB2 phase of the ceramic suface is entirely oxidized. The oxidation layer thickness is increased with an increase in the oxidation time, however, the ratio of increasing thickness is less and less. The oxidation layer shows a loose structure during the initial stage of the oxidation, which lacks a barriers for the diffusion of the oxygen.

Effect of raw materials on the properties of ZrB2-YAG-Al2O3 multi-phase ceramics

Jie-Guang Song,Fang Wang,Da-Ming Du,Yin-Yan Ju,Shi-Bin Li,Gang-Chang Ji,Lian-Meng Zhang 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.2

ZrB2 belongs to a class of ceramics defined ultra-high-temperature ceramics with extremely high melting temperatures, but ZrB2 ceramics is difficultly sintered and easily oxidized. To make ZrB2 ceramics possess the high relative density and the better oxidation resistance. The effects of raw materials on the properties of ZrB2 composite were investigated. YAG and Al2O3 help for the densification of ZrB2 ceramics. Fracture toughness of sintered ceramics with coated powder is higher than that of sintered ceramics with mixed powder. The mechanical property of ZrB2-YAG-Al2O3 materials is higher than that of ZrB2-YAG materials. Oxidation layer thickness of sintered ceramics with coated powder is thinner than that of sintered ceramics with mixed powder. These results show the sintered ZrB2 based multi-phase ceramics with coated microstructure help to increase the mechanical properties and oxidation resistance. The oxidation resistance of ZrB2-YAG-Al2O3 ceramics is better than that of ZrB2-YAG materials.