척추 경막외 농양의 임상분석

손동욱 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

老齡者의 建康을 위한 生活習慣 實態調査

申東敏,長鳳愚,李揆文,金賢俊,金昌範,崔宗洙,崔鍾晥,李鍾珏,金圭碩,趙庚旭,金鍾聲,朴鍾振 平生體育硏究所 論文集 1987 平生體育硏究所 論文集 Vol.2 No.-

With a view to examining the habit and method in which old people maintain and improve their health, we consulted 551 men and 444 women who were over 65 years old, and concluded as follows: 1. Habit of eating It's been revealed that most of the old people keep a regular eating hour, control the quantity of meal or eat a little food, and prefer vegetables to meat. 2. Mental health Old people feel the necessity for mental health and most of them (about 80%) keep good mental health without becoming pessimistic. Their chief concenrs have turned out to be about their sons and daughters and their own health and finance. 3. Managing Good Health It has been revelaed that old people do not possess any special kind of secret to keep good health but taking plenty of rest, regular meal, and proper exercise, and many of them (about 60%) have been shown to eat invigorant food that they can easily get around their living area. 4. Physical Functions It has been revealed that many old people are conscious of some symptoms about their parts of body and about to percent of them suffers from neuralgia. Some measures like medical aid especially for old people need to be taken as soon as possible. As we have found that the health-keeping method of old people consists plenty of rest, proper exercise, and appropriate nutrition, we can conclude that the recreatonal and culture facilities and programs for keeping old people in good health should be developed. It is also necessary to study food that contribute to their health and develop ways to get rid of the stresses to which they are exposed in their daily life. Presides, it is natural that government found an administrative department to deal with these matters effectively.

RFLP법을 이용한 사상체질의 유전적 분석 연구

趙東郁,趙皇晟 현곡학회 1997 제3의학 Vol.2 No.1

In Sasang medicine, humen are classified into four constitutions which are Taevang, Sovang, Taeum and Soum. Depending on each different constitution, the clinical and pharmacological application for the same disease might be different. In this study, genomic DNA of different constitutions(Taeum, Soyang and Soum) were analyzed by Restriction Fragment Length Polymorphism(RFLP) to provide scientific and objective references for Sasang classification. The DNA polymorphism for each constitution detected as differences in the length of DNA fragments, after digestion with restriction enzyme Hae III, was investigated using YNH24 as DNA probe. The allele size of Taeum, Soyang and Soum group detected by YNH24 ranged from 1.3 to 3.8 kb, 1.5 to 3.9 kb and 1.3 to 4.6 kb, respectively. However, the allele size distribution of YNH24 loci of different constitutions was shown to be too variable to be classified as 3 different constitution groups investigated. For further study, it is suggested that the number of each constitution samples for RFLP analysis should be increased and statistical analysis of the allele size distribution should be carried out.

RAPD 다형성 및 ABO 유전자형 분석을 통한 사상체질간 유전적 거리에 관한 연구

이휘철,조동욱,조중호,이창수 건국대학교 자연과학연구소 1999 建國自然科學硏究誌 Vol.10 No.2

본 연구는 사상체질인 태음인, 소음인 그리고 소양인간의 유전적 상관관계를 Random Amplified Polymorphic DNA(RAPD) 분석법 및 ABO 유전자형의 대립유전자의 빈도 분석을 통하여 실시하였다. 체질간 DNA 다형성을 검출할 수 있는 RAPD 분석용의 primer의 빠른 선별을 위해 체질별 혼합된 DNA 시료를 분석에 사용하였다. 일차적으로 200종류의 RAPD primer로부터 다형성을 보이는 7종류의 primer를 선별하였다. RAPD밴드로터 체질간 bandsharing(BS) 값은 0.68에서 0.71범위에 있었고, 유전적 거리는 BS 값에 의해 구했다. RAPD의 BS값에 의한 태음인과 소음인간 유전적 거리는 0.002이고, 이 두 체질과 소양인간에는 0.005로 분석되었다. 또한 체질별 ABO 유전형을 조사하여 그들의 대립유전자 빈도를 추정해 체질간 유전적 거리를 산출했다. 그 결과 태음인과 소음인사이에 유전적 거리는 0.014이고, 소양인과 나머지 두 체질간에는 0.032였다. 위와 같이 RAPD 분석법과 ABO 대립유전자의 빈도분석에 의한 체질간 상관관계는 두 방법 모두에서 태음인과 소음인이 소양인 체질보다 유전적으로 더 가깝게 나타났다. This study was carried out to be establish genetic understanding of Sasang constitutions of Teaumin, Soumin and Soyangin by Random Amplified Polymorphic DNA(RAPD) and ABO alleles frequencies analysis. We have applied RAPD analysis to pooled DNA sample as a means to achieve rapid screening of large numbers of primers for their capacity to reveal constitutions-specific polymorphisms. From initial 200 primers, 7 polymorphic primers between different constitutions were selected. The RAPD bandsharings(BS) values ranged from 0.68 to 0.71 for between three constitutions. The genetic distance between three constitutions was measured by BS values. Genetic distance by RADP analysis was 0.002 between Teaumin and Soumin, and 0.005 between Soyangin and the others. Three Sasang constitutions were investigated by the genotyping of ABO genotypes, and genetic distance was calculated from ABO allele frequencies in each constitution. As a result, genetic distance was 0.014 between Teaumin and Soumin, and 0.032 between Soyangin and the others. In conclusion, the genetic distance of Teaumin and Soumin was closer than that of Soyangin in the analysis of RAPD and ABO alleles frequencies.

고추의 品質을 결정하는 成分形質에 對한 Heterosis表現과 組合能力

朴載福,韓東旭 서울市立大學校 1984 論文集 Vol.18 No.-

Since the discovery of genetic-cytoplasmic made sterility of hot pepper, many F₁varieties were come out in Korea. Thus, to clarify the inheritance mode of some important quality characters of hot pepper, heterosis, combining ability and gene actions were estimated with diallel crosses among 6 hot pepper varieties. The materials were grown in the field and were served for genetic analysis. The results of F₁diallel cross for some quality characters were as follows. 1. In carotenoid content, significant heterosis was showed, while it was not showed in capsaicin, vitamine C, sugar and free amino acid content. 2. The continuous variation was obserbed in the quality characters of hot pepper such as capsaicin, carotenoid, vitamine C, sugar, and free amino acid content. 3. Besides the additive control, some combinations showed the epistasis or complementary control of polygene, and dominant major gene seemed to be effect, too. 4. The mean squares of general combining ability (GCA) for all studied characters were highly significant, but those of specific combining ability (SCA) were not significant. 5. Capsaicin content were positively correlated with carotenoid content while it was not negatively correlated with vitamin C, sugar, and free amino acid content. 6. Fruit weight was negatively correlated with capsaicin and carotenoid content, while it was negatively correlated with vitamine C, sugar, and free amino acid content. 7. To improve the F₁ hybrid with large fruit and high capsaicin content, selection of breeding materials was indispensable problem. This work was also showed possibility to breed the high quality varieties contained a plenty of vitamine C and amino acid.

뇌출혈 동물모델에서 육미지황원의 효과 연구

강봉주,조동욱 한국한의학연구원 2001 한국한의학연구원논문집 Vol.7 No.1

Intracerebral hemorrhage(ICH) is associated with a considerable proportion of strokes and head injuries. The mechanism of brain cell injury associated with hemorrhage may be different from that due to pure ischemia. Therefore, it is essential that models of intracerebral hemorrhage be developed and well characterized in animal model. Yukmijihwangwon (YM) has been known to reinforce the vital essence and have antioxidant activities. In this study, the protective effects of YM was investigated against ICH in animal models. Adult rats had 2 microliters saline containing 0.5 and 5 unit bacterial collagenase ifused into the right caudate nucleus. It was found out that YM was effective in protecting brain against ICH.

참깨 立枯性 病原菌에 관한 硏究

李斗珩,梁壯錫,韓東旭 서울市立大學校 1986 論文集 Vol.20 No.2

Damping-off and wilting diseases in sesame plant(Sesamum indicum L.) occurring severely in Korea have been studied for incidences of disease from seeds, diseased plants and soils. Alternaria longissima, A. tenuis, Aspergillus. spp, Cephalosporium sp., Cuvuaria lunata, Drechslera sp.,sp., Fusarium equiseti, F. moniliforme, F. semitectum, F. solani, Mucor sp., Myrothecium roridum Penicillium spp., Phoma sp., Rhizopus sp., were saprophytic fungi and Alternaria sesami, A. sesamicola, Macrophomina phaseolina, Rhizoctonia solani were pathogenic fungi detected from 66 seed samples of sesame. Initial symptoms caused by pathogenic fungi on seedlings from naturally infected seed by the test tube agar method have been described and discussed. Except Alternaria sesamicola, A. tenuis and Macrophomina phaseolina, all fungi were completey wiped out the infection by pretreatment with Benlate-T. The pathogenic fungi isolated from sesame soil were Fusarium oxysporum and Rhizoctonia solani. F.oxysporum occurred less frequently in upland rice and ground nut soils than in sesame soil, but R. solani not decreased by upland rice and ground nut soils. The differences of the isolation frequency of F.oxysporum and the incidence of the wilting plants were found among the fields which varied in their cropping histories. F.oxysporum isolated most frequently from stems and roots of wilting sesame plants followed by R. solani. Nematodes also isolated from the wilting sesame plants.

저산소상태에서 육미지황원의 뇌신경세포 보호효과에 대한 연구

강봉주,홍성길,조동욱 한국한의학연구원 2001 한국한의학연구원논문집 Vol.7 No.1

Yukmijinhwangwon(YM) has been known to reinforce the vital essence and have antioxidant activities. This study was designed to examine the inhibitory effects of YM against in vitro hypoxia/reperfusion-induced inflammatory response. We have characterized the production of prostaglandin E₂and arachidonic acid during hypoxia/reperfusion in the human neuroblastoma SK-N-MC and human monocytic macrophage U937 cells and the ingibitory effect of YM on these inflammation-related substance formation has been found out in this study. To investigate inhibition of COX expression by YM during hypoxia in vitro. This result suggested that YM used in this experiment reinforced antiinflammatory potentials and protected cells against hypoxia/reperfusion induced inflammatory response.

한국인의 신뢰 수준 및 영향 요인 : 단체참여 및 개인의 사회,경제적 배경과 대인신뢰, 단체신뢰, 대중매체 신뢰

박희봉,이희창,김동욱,김철수,박병래 한국정책학회 2003 韓國政策學會報 Vol.12 No.3

본 연구는 한국인의 신뢰수준과 단체참여를 중심으로 신뢰에 영향을 미치는 요인을 분석하여, 이를 토대로 신뢰 증진을 위한 방안을 논의하는데 목적이 있다. 이를 위하여 대인신뢰, 단체신뢰, 대중매체신뢰와 관견된 23개 항목에 대한 신뢰를 Likert 5점 척도로 각각 질문하여 항목별 평균을 신뢰 측정치로 이용하였다. 각종 신뢰 변수와 단체참여 변수는 요인분석을 통하여 유형화 하였다. 본 연구의 측정을 위한 모집단은 우리나라 14개 광역 자치단체에 거주하는 20세 이상의 성인이다. 분석 결과 우리나라 국민들의 전반적인 신뢰수준은 유형별 큰 편차를 보였다. 즉 개인적 네트워크를 통해 이미 알고 있는 지인에 대한 사적신뢰는 매우 높은 반면 개인적인 친분이 없는 일반인 신뢰는 매우 낮게 나타났고, 사회단체에 대한 신뢰와 대중매체 신뢰는 비교적 높게 나타난 반면 공공기관에 대한 신뢰는 상대적으로 낮게 나타났다. 한편, 수평적 단체 및 수직적 단체 변수가 대인 및 단체 신뢰에 영향을 미치고 있었다. 대부분의 단체참여가 각종 신뢰에 영향을 미치고 있음을 나타내었다. 개인의 사회경제적 배경에 따라 긍정적 또는 부정적 형태로 다양한 영향을 나타내었다. 이는 우리나라가 아직 공동사회에 기초한 신뢰에 의존하고 있어 시민사회 및 2차사회 중심의 신뢰가 제대로 형성되지 못하고 있음을 시사하고 있다. 본 연구에서는 이러한 결과를 토대로 신뢰증진의 필요성에 관해 논의하였다. This study examines the trust levels of Korean people, and clarifies the factors to affect the Korean people's trust. For the purpose of this study, how the factors including horizontal association participation, hierarchical association participation and individuals' socio-economic status affect Korean people's trust is analyzed. More than 7,000 surveys are used for data in 14 provinces in Korea. The major findings of this study as followings : First, the degree of Korean people's trust has distinct difference according to the relationship with them and the subjects of the trust. Korean people show high levels of trust for their family members, friends and colleagues who they know already, while they hardly trust the general people. They show comparatively high levels of trust for civil association and mass media, while they show comparatively low levels of trust for public institutions. Second, hierarchical social association participation as well as the horizontal participation positively affect personal, associational, and meas medial trust. Third, according to the individuals' socio-economic status, the levels of trust shows strikingly contrast. Some variables of individuals' socio-economic status positively affect some kinds of trust, and others negatively affect other kinds of trust. This means that Korean peoples' trust is formed mainly by the gemeinschaft, not by the gesellschaft yet. Overall, this paper discusses how to improve various kinds of trust and suggests research implications for further research.

RAPD(Random Amplified Polymorphic DNA)법을 이용한 한약재의 판별 연구

김대원,김도균,안선경,조동욱 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition. Using these methods, however, is not satisfying for the precise identification of natural products because they are often variable in the compositions, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notohinseng, were genetically analyzed by RAPD. Also, DNA isolated form Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctors, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification of other natural products.