Identification of thioredoxin domain-containing protein 17 from big-belly seahorse <i>Hippocampus abdominalis</i>: Molecular insights, immune responses, and functional characterization

Liyanage, D.S.,Omeka, W.K.M.,Yang, Hyerim,Godahewa, G.I.,Kwon, Hyukjae,Nam, Bo-Hye,Lee, Jehee Elsevier 2019 Fish & shellfish immunology Vol.86 No.-

<P><B>Abstract</B></P> <P>Thioredoxin domain-containing protein 17 (TXNDC17) is a small protein (∼14 kDa) involved in maintaining cellular redox homeostasis via a thiol-disulfide reductase activity. In this study, TXNDC17 was identified and characterized from <I>Hippocampus abdominalis</I>. The open reading frame (ORF) consisted of 369 bp and 123 amino acids. Similar to the other thioredoxins, TXNDC17 contained a conserved WCXXC functional motif. The highest spatial mRNA expressions of <I>HaTXNDC17</I> were observed in the muscle, brain, and intestine. Interestingly, the mRNA expression of <I>HaTXNDC17</I> in blood showed significant upregulation at 48 h against all the pathogen associated molecular patterns (PAMPs) and bacteria. Further, <I>HaTXNDC17</I> transcripts in the trunk kidney were significantly upregulated at 24–48 h by bacterial endotoxin lipopolysaccharides (LPS), viral mimic polyinosinic: polycytidylic acid (poly I:C), and gram-negative bacteria (<I>Edwardsiella tarda</I>). The DPPH assay showed that the radical scavenging activity varies in a concentration-dependent manner. The insulin reduction assay demonstrated a significant logarithmic relationship with the concentration of rHaTXNDC17. Moreover, FHM cells treated with recombinant HaTXNDC17 significantly enhanced cellular viability under oxidative stress. Together, these results show that HaTXNDC17 function is important for maintaining cellular redox homeostasis and that it is also involved in the immune mechanism in seahorses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Big-belly seahorse thioredoxin domain containing protein 17 maintain redox homeostasis. </LI> <LI> TXNDC17 is ubiquitously found in cytosol and extracellular space. </LI> <LI> Thiol active CXXC conserved motif consists in HaTXNDC17. </LI> <LI> Spatial and temporal mRNA expression was evaluated. </LI> <LI> Radical scavenging ability, antioxidant activity and cellular viability were measured. </LI> </UL> </P>

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

비타민 D3 의 경구투여 또는 자외선 조사가 브로일러 병아리의 증체 및 비타민 D3 대사에 미치는 영향

장윤환(Y . H . Chiang),황선일(S . I . Hwang),김중달(J . D . Kim),M . F . Holick(M . F . Holick) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.6

한국산 하루살이목(目)의 카탈로그

윤일병,배연재,전동준 고려대학교 한국곤충연구소 1994 昆蟲硏究誌 Vol.20 No.1

韓國産 하루살이目의 카탈로그. 裵淵宰 · 尹一炳^1 · 田東埈^2 (서울女子大學校, ^1高麗大學校 生物學科, ^2러시아科學院 動物學硏究所) 昆蟲硏究誌, 20: 31~50, 1994. 韓國産 하루살이目의 關한 硏究는 南北韓에 걸친 綜合的인 硏究가 이루어지지 못하였고, 1940년 以來 各種 文獻에 記錄된 種들에 있어서도 分類上 혹은 命名上 變動이 많았으나 이를 體系的으로 收錄하지 못하였다. 이 論文에서는 種合的인 文獻 및 模式標本 調査를 통하여 다음과 같이 韓國産으로 記錄된 56種의 命名상 有效한 種들에 대한 카탈로그를 작성하였다. Acerella longicaudata(U′eno), *Ameletus costalis (Matsumura), A. montanus Imanishi, *Baetiella japonica (Imanishi), *B, tuberculata (Kazlauskas), *Baetis thermicus U′eno, Bleptus fasciatus Eation, Choroterpes altioculus Kluge, Cincticostella castanea (Allen), C. tshernovae(Bajkova), * Cinygmula grandifaliz Tshernova, *C. hirasana (Imanisht), Cloeon dipterum (Linnaeus), Drunella aculea (Allen), *D. cryptomeria (Imanishi), *D. lepnevae (Tshernova), D. triacantha (Tsher-nova), *D. trispina ezoensis (Gose), Ecdyonurus bajrovae Kluge, E. dracon Kluge, **E. Joernensis mon-golicus Bajkova & Varychanova, E. kibunensis Imanishi, E. levis (Nava′s), E. scalaris Kluge, **E. subspinosa (Braasch & Solda′n), Epeorus curvatulus Matsumura, *E. hiemalis Imanishi, E. latifolium Ue′no, E. pellucidus (Broadsky), *Ephemeralla aurivillii Bengtsson, *E. imanishii Gose, E. keijoensis Allen, *E. notofascia Yoon & Base, Ephemera orientalis McLachlan, E. separigata Bae, E. strigata Eaton, Ephoron shigae (Takahashi), Heptagenia guranica Belov, H. kihada Matsumura, *H. Kyotoensis Gose, *Iron aesculus (Imanishi), *I. koreanicus Braasch & Solda′n, I. maculatus Tshernova, Isonychia japonica (Ulmer), **I. ussurica Bajkova, Paraleptophlebia chocolata Imanishi, *Potamanthellus rarus tiunova & Levanidova, **Potamanthus formosus Eaton, P. yooni Bae & McCafferty, *P. luteus oriens Bae & McCafferty, *Phithrogena japonica Ue′no, Rhoenanthus coreanus (Yoon & Bae), *Serratella setigera (Bajkova), Siphlonurus chankae Tshernova, *S, sanukensis Takahashi 및 Uracanthella rufa (Imanishi). 별표한 種들은 幼蟲만이, 쌍별표한 種들은 成蟲만이 韓國産으로 記載되었다. (C. tshernovae와 E. keijoensis는 암컷 成蟲과 幼蟲만이, 그리고 P. luteus oriens는 亞成蟲만이 韓國産으로 記載되었다.) 다음 10종은 韓國에서 幼蟲만이 記載되었고, 아직 命名上으로 留保된 未決定種으로 남아있다. Baetis Kua: Yoon & Bae, Baetis nla: Imanishi, Brachycercus Kua: Yoon & Bae, Caenis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Cinygmula na: Imanishi, Ecdyonurus Kub: Yoon & Bae, Ecdyonurus na: Imanishi, Paraleptophlebia nb: Imanishi 및 Rhithrogena na: Imanishi. 각 種에 있어서 原記載 및 再記載 文獻, 記載에 使用된 標本, 分布, 模式標本에 대한 情報, 異名과 命名上의 變動을 年代順으로 整理하였다. 附錄으로 南韓種만의 checklist와 分類學的 變動 및 國名의 變動事項을 表로 作成하였다. Mayflies (Ephemeroptera) of Korea have been described in various literatures since 1940, but there have been many taxonomic or nomenciatural changes in the members of the order. A comprehensive literature and type material review of the Ephemeroptera resulted herein the recognition of 56 nomenclaturally valid species previously described from Korea : Acerella longicaudata (Ue´no), ^*A meletus costalis (Matsumura), A. montanus Imanishi, ^*Baetis thermicus Ue´no, Bleptus fasciatus Eaton, Choroterpes altioculus Kluge, Cincticostella castanea (Allen), C. tshernovae (Bajkova), ^*Cinygmula grandifolia Tshernova, ^*C. hirasana (Imanishi), Cloeon dipterum (Linnaeus), Dyunella aculea (Allen), ^*D. cryptomeria (Imanishi), ^*D. tepnevae (Tshernova), D. triacantha (Tshernova), ^*D. trispina ezoensis (Gose), Ecdynurus bajrovae Kluge, E. dracon Kluge, ^*^*E. joernensis mongolicus Bajkova & Varychanova, E. kibunensis Imanishi, E. levis (Nava´s), E. scalaris Kluge, ^*^*E. subspinosa (Braasch & Solda´n), Epeorus curvatulus Matsumura, ^*E. hiemalis Imanishi, E. latifolium Ue´no, E. notofascia Yoon & Bae, Ephemera orientalis McLachlan, E. separigata Bae, E. strigata Eaton, Ephoron shigae (Takahashi), Heptagenia guranica Belov, H. kihada Matsurnura, ^*H. kyotoensis Gose, ^*Iron aesculus (Imanishi), ^*I. Koreanicus Braaich & Solda´n, I. Maculates Tshernova, Isonychia japonica (Ulmer), ^*^*I. Ussurica Bajkova, Paraleptophlebia chocolate Imanishi, ^*Potamanthellus rarus Tiunova & Levanidova, ^*^*Potamanthus formosus Eaton, P. yooni Bae & McCafferty, ^*^*P. luteus oriens Bae & MeCafferty, ^*Rhithrogena japonica Ue´no, Rhoenanthus coreanus (Yoon & Bae), ^*Serratella setigera (Bajkova), Siphlonurus chankae Tshernova, ^*S. sanukensis Takahashi, and Uracanthella rufa (Imanishi), The asterisked species were known to larva only from Korea, and the double asterisked were adults only (C. tshernovae and E. keijoensis were females and larvae only, and P. luteus oriens was subimagos only), The following 10 species were known to larvae only from Korea, but have not been determined: Baetis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Baetis nla: Imanishi, Brachycercus Kua: Yoon & Bae, Caenis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Cinygmula na: Imanishi, Ecdyonurus Kub: Yoon & Bae, Ecdyonurus na: Imanishi, Paraleptophlebia nb: Imanishi, and Rhithrogena na: Imanishi. For each species, original and secondary sources of the descriptions, materials used, type information, distributions, synonyms, aid nomenclatural changes were chronologically provided. A checklist of the Ephemeroptera previously recorded from South Korea was appended with their proposed Korean name.

Cytogenetic assessment of <i>Lilium longiflorum × L. hansonii</i> revealed by genomic in situ hybridization (GISH)

Mazharul, I.M.,Reshma, Y.,Jung, J.M.,Mohammad, D.D.,Lim, K.B. International Society for Horticultural Science 2019 Acta Horticulturae Vol.1237 No.-

<P> Martagon (<I>Lilium hansonii;</I> MM) and <I>Longiflorum</I> (LL) are two major groups under the family <I>Liliaceae</I>, used for modern breeding to introduce new inter-genomic lily cultivars. Interspecific F<SUB>1</SUB> hybrids (LM) introduced through cut-style method between two diploid <I>Lilium longiflorum (2n=2x=24)</I> and <I>Lilium hansonii (2n=2x=24)</I> were evaluated cytogenetically by genomic in situ hybridization (GISH) technique. However, GISH analysis of F<SUB>1</SUB> interspecific (LM) hybrids showed equal chromosomal contribution from both female <I>Lilium longiflorum</I> (LL) and male <I>Lilium hansonii</I> (MM). Each of the parent contributed 12 chromosomes except three crosses i.e., two of <I>L. longiflorum</I> 'White Tower' × <I>L. hansonii;</I> (2x-1) and one of <I>L. longiflorum</I> 'Bright Tower' × <I>L. hansonii;</I> (2x-1). Among 11 inter-genomic crosses, 3 crosses failed (False hybrid) and 8 crosses (True hybrids) showed different ploidy level i.e., 2n=2x=24, 2n=2x-1=23 and 2n=2x-1=23 respectively. Recombinant chromosome usually not found in F<SUB>1</SUB> interspecific lily hybrids. Most often, genomic recombination occurred in the cross between two genetically different parents. Chromosome pairing and crossing over normally occurred during meiosis in backcross progenies. However, in this study, genome analysis (GISH) of F<SUB>1</SUB> hybrids (<I>L. longiflorum</I> 'White Tower' × <I>L. hansonii</I>) showed four recombinant sites including two M/L and two L/M recombinant chromosomes that denotes high genetic relationship between <I>L. longiflorum</I> and <I>L. hansonii.</I> </P>

Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

The Effect of Spray Dried Plasma, Lactose and Soybean Protein Sources on the Performance of Weaned Pigs

Liu, H.,Kim, I.B.,Touchette, K.J.,Newcomb, M.D.,Allee, G.L. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.9

A total of 371 weaned pigs were used in three experiments to evaluate the effects of spray dried plasma (SDP), soybean protein sources, and lactose on growth performance. In Exp. 1, 128 pigs (5.99 kg, $18{\pm}2d$) were used to evaluate the effect of SDP (0 vs 7%), lactose (0 vs 30%), and two soybean protein sources [soybean meal (SBM) and extruded soybean protein concentrate (ESPC)] in phase I (d 0 to 14) diets on pig performance in a $2{\times}2{\times}2$ factorial arrangement. Spray-dried plasma increased phase I ADG (p<0.01) and ADFI (p<0.05) in the SBM diets, but not in the ESPC diets. Lactose improved ADG and gain/feed ratio (G/F) in phase I (p<0.01). In Exp. 2, 144 pigs (5.50 kg, $17{\pm}3d$) were used to evaluate the effect of SDP (0 vs. 3.5%) and three soybean protein sources [SBM, ESPC, and soybean protein concentrate (SPC)] in phase I diets, and the effects of two different phase II (d 14 to 28) diets (simple vs complex) in a $2{\times}3{\times}2$ factorial arrangement of treatments. In phase I, SDP increased ADG (p<0.01) and improved G/F (p<0.05). Pigs fed SBM had the highest ADG and ADFI, with a G/F similar to the pigs fed ESPC. In phase II, pigs fed the complex diet had improved ADG (p<0.01), ADFI (p<0.05), and G/F (p<0.05) compared to the simple diet. In Exp. 3, 99 weaned pigs (5.77 kg, $17{\pm}3$d) were used to evaluate the effect of SBM, ESPC, and ESPC with SDP in the phase I diets. Pigs fed SBM with no blood product in the diet had the lowest ADG (p<0.01), ADFI (p<0.01), and G/F (p<0.05) in the first week of phase I. There were no differences in soybean protein sources fed in phase I diets on overall pig performance. These experiments are indicated that SDP and lactose improve the phase I performance. Soybean meal can be used as the major protein source in phase I diets with SDP.

Resource allocation for device-to-device communications underlaying LTE-advanced networks

Phunchongharn, P.,Hossain, E.,Kim, D. I. IEEE 2013 IEEE wireless communications Vol.20 No.4

<P>The Long Term Evolution-Advanced (LTEAdvanced) networks are being developed to provide mobile broadband services for the fourth generation (4G) cellular wireless systems. Deviceto- device (D2D) communications is a promising technique to provide wireless peer-to-peer services and enhance spectrum utilization in the LTE-Advanced networks. In D2D communications, the user equipments (UEs) are allowed to directly communicate between each other by reusing the cellular resources rather than using uplink and downlink resources in the cellular mode when communicating via the base station. However, enabling D2D communications in a cellular network poses two major challenges. First, the interference caused to the cellular users by D2D devices could critically affect the performances of the cellular devices. Second, the minimum quality-of-service (QoS) requirements of D2D communications need to be guaranteed. In this article, we introduce a novel resource allocation scheme (i.e. joint resource block scheduling and power control) for D2D communications in LTE-Advanced networks to maximize the spectrum utilization while addressing the above challenges. First, an overview of LTE-Advanced networks, and architecture and signaling support for provisioning of D2D communications in these networks are described. Furthermore, research issues and the current state-of-the-art of D2D communications are discussed. Then, a resource allocation scheme based on a column generation method is proposed for D2D communications. The objective is to maximize the spectrum utilization by finding the minimum transmission length in terms of time slots for D2D links while protecting the cellular users from harmful interference and guaranteeing the QoS of D2D links. The performance of this scheme is evaluated through simulations.</P>

A systematic investigation of the thermoelectric stability of Pt–Rh thermocouples between 1300 °C and 1500 °C

Pearce, J V,Edler, F,Elliott, C J,Greenen, A,Harris, P M,Izquierdo, C Garcia,Kim, Y-G,Martin, M J,Smith, I M,Tucker, D,Veltcheva, R I BUREAU INTERNATIONAL DES POIDS ET MESURES 2018 METROLOGIA -BERLIN- Vol.55 No.4

<P>By using a simple model to relate the electromotive force drift rate of Pt–Rh thermoelements to d<I>S</I>/d<I>c</I>, i.e. the sensitivity of the Seebeck coefficient, <I>S</I>, to rhodium mass fraction, <I>c</I>, the composition of the optimal pair of Pt–Rh wires that minimizes thermoelectric drift can be determined. The model has been applied to four multi-wire thermocouples each comprising 5 or 7 Pt–Rh wires of different composition. Two thermocouples were exposed to a temperature of around 1324 °C, one thermocouple to around 1492 °C, i.e. the melting points of the Co–C and Pd–C high temperature fixed points, respectively, and one thermocouple to a series of temperatures between 1315 °C and 1450 °C. The duration of exposure at each temperature was several thousand hours. By performing repeated calibrations <I>in situ</I> with the appropriate fixed point during the high temperature exposure, the drift performance has been quantified with high accuracy, entirely free from errors associated with thermoelectric homogeneity. By combining these results it is concluded that the Pt-40%Rh versus Pt-6%Rh is the most stable at the temperatures investigated. A preliminary reference function was determined and is presented.</P>

Potential roles of D-serine and serine racemase in experimental temporal lobe epilepsy

Ryu, H.J.,Kim, J.-E.,Yeo, S.-I.,Kim, D.-S.,Kwon, O.-S.,Choi, S.Y.,Kang, T.-C. Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of neuroscience research Vol.88 No.11

<P>To confirm the roles of D-serinergic gliotransmission in epilepsy, we investigated the relationship between spatiotemporally specific glial responses and the D-serine/serine racemase system in mesial temporal structures following status epilepticus (SE). In control animals, D-serine and serine racemase immunoreactivities were detected mainly in astrocytes. After SE, D-serine and serine racemase immunoreactivities were increased in astrocytes. Double-immunofluorescence study revealed that up-regulation of serine racemase immunoreactivity was relevant not to D-serine immunoreactivity but to nestin or vimentin immunoreactivity. Neither D-serine nor serine racemase was found in naïve or reactive microglia. In addition, phosphorylated N-methyl-D-aspartate (NMDA) receptor subunit 1 (pNR1-Ser896) immunoreactivity in the hippocampus was increased compared with controls. Increased D-serine immunoreactivity showed direct correlation with the phosphorylation of Ser896 of NR1. Given the findings of our previous study, these findings suggest that D-serine and serine racemase in astrocytes may play roles in neuronal hyperexcitability via a cooperative activation of NMDA receptors. Furthermore, serine racemase may be involved in migration and differentiation of immature astrocytes, which is relevant to reactive astrogliosis. © 2010 Wiley-Liss, Inc.</P>