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Bao Yanan,Cui Yue,Luan Yumin 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.2
Background Circular RNA hsa_circ_0011292 (circ_0011292) participates in NSCLC resistance to paclitaxel (PTX). Here, we further clarify the mechanism of circ_0011292 regulating non-small cell lung cancer (NSCLC) resistance to PTX. Objectives Expression patterns of circ_0011292, microRNA (miR)-3619-5p, and CDCA4 mRNA were verifi ed by qRT-PCR. The IC 50 (half-maximal inhibitory concentration) value, proliferation, migration, invasion, and apoptosis were analyzed by MTT, transwell, and fl ow cytometry assays. Protein levels were measured by western blotting (WB). The regulation mechanism of circ_0011292 was analyzed by bioinformatics analysis, dual-luciferase reporter, and/or RNA pull-down assays. Results Circ_0011292 was highly expressed in PTX-resistant NSCLC. Functionally, circ_0011292 inhibition lowered cell IC 50 value, induced cell apoptosis, and repressed cell proliferation, migration, and invasion in PTX-resistant NSCLC cells in vitro. Mechanically, circ_0011292 acted as a decoy for miR-3619-5p, which targeted CDCA4 in PTX-resistant NSCLC cells. Both miR-3619-5p inhibitor and CDCA4 overexpression overturned circ_0011292 inhibition-mediated infl uence on PTX sensitivity and malignant behaviors of PTX-resistant NSCLC cells. Importantly, circ_0011292 adsorbed miR-3619-5p to regulate CDCA4 expression. Conclusions Circ_0011292 facilitates PTX resistance in NSCLC partially through the miR-3619-5p/CDCA4 pathway, highlighting a new mechanism responsible for PTX resistance in NSCLC.
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Development and Validation of a Low-Cost and Simple Simulator for Microlaryngeal Surgery
Pengcheng Yu,Jia Luan,Xidong Cui,Xumao Li,Xinqi Hu,Guangbin Sun 대한이비인후과학회 2020 Clinical and Experimental Otorhinolaryngology Vol.13 No.1
Objectives. The simulation of microlaryngeal skills is rarely seen in surgical training, but it is particularly important in phonomicrosurgery. This study described and validated the laryngeal surgical simulator through surgical training. Methods. A simple and low-cost simulator was developed for the fixation of the suspension laryngoscope and porcine larynges. Twenty participants with work skills and experience did preparation before training, and performed suture and carbon dioxide (CO2) laser cordectomy for simulator evaluation. The results were proposed by the aspects of time taken for each procedure, the global rating scale, a procedure-specific assessment, and a post-simulation questionnaire. Results. All participants completed the preparation within 9 minutes and reached the conclusion that the microlaryngeal surgical simulator was helpful in improving their surgical skills. The performance of experts was superior to that of novices in both suture and CO2 laser cordectomy. Conclusion. This simulator could be easily assembled and was successfully validated by microlaryngeal surgical training both subjectively and objectively. It may be helpful to clinicians in microlaryngeal skills.
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Yu-Cai He,You-Yan Liu,Cui-Luan Ma,Jian-He Xu 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5
We successfully modified a ferric hydroxamate spectrophotometry method for assaying glycolic acid. Comparable to the high-performance liquid chromatography (HPLC)-based method, ferric hydroxamate spectrophotometry can be used to accurately monitor the time course of glycolonitrile bioconversion. Glycolic acid was assayed simply and rapidly at room temperature (25 ~35℃). Optimum culture conditions were obtained using this method to assay the glycolonitrile-hydrolyzing activity of Rhodococcus sp. CCZU10-1. The preferred carbon and nitrogen sources and ideal inducer were glucose (10 g/L),a composite of peptone (10 g/L) plus yeast extract (5 g/L),and ε-caprolactam (2 mmol/L), respectively. The optimal growth temperature and initial medium pH for Rhodococcus sp. CCZU10-1 glycolonitrile-hydrolyzing activity were 30℃ and pH 7.0. Modified ferric hydroxamate spectrophotometry could potentially be employed to assay other carboxylic acids.
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