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( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.
Xue-fen Wan(만설분),Yi Yang(양의),Jian Cui(최검),Tao Zheng(정도),Li Ma(마려) 대한전자공학회 2012 전자공학회논문지 Vol.49 No.12
본 논문에서는 중국 현지 염색 기업의 열 센서망에 적용 가능한 원격 가상 현실 제어 플랫폼에서 실행할 수 있는 데이터베이스 액세스를 가상 현실 모델링 언어 (VRML)를 소개하였다. 또한, 3차원 실시간 자료 시각화를 위한 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP)를 소개하였다. 나아가 이와 관련된 스크립트도 다루었을 뿐만 아니라, 열 센서 노드와 센서 영역에서의 움직임을 분석하였다. 이 데이터베이스 프레임워크는 마이크로칩에서 발표한 마이와이<SUP>TM</SUP>(MiWi<SUP>TM</SUP>)의 표준에 최적화되었다. 이러한 분석 결과를 근거로, 시스템 구성에 따라 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP) 데이터 액세스 프레임워크가 가상 현실 원격 산업 공정 제어 시스템을 위한 하나의 경쟁력 있는 데이터 관리 해결책이 될 수 있음을 확인할 수 있었다. A Virtual Reality Modeling Language (VRML) database access in remote virtual reality control platform for dyeing enterprise MiWi<SUP>TM</SUP> thermal sensor network is presented in this paper. The VRML-ASP framework is introduced for 3D real-time data plotting in this application. The activities of thermal sensor nodes and sensor area are analyzed. The database access framework is optimized for MiWi<SUP>TM</SUP> wireless sensor networks. The experimental results show that VRML-ASP database access framework could be a reliable and competitive data-manage candidate for targeted virtual reality remote industrial visualization application.
Yang, Xue-Xi,Li, Fen-Xia,Zhou, Cui-Ping,Hu, Ni-Ya,Wu, Ying-Shong,Li, Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
Objective: Data from a recent genome-wide association studiesy of gastric cancer (GC) and oesophageal squamous cell carcinoma in Chinese living in the Taihang Mountains of north-central China suggest that 1q22 and 10q23 are susceptibility-associated regions for GC. However, this has not been confirmed in southern Chinese populations. The aim of this study was to investigate whether these polymorphisms at 1q22 and 10q23 are associated with the risk of GC in a southern Chinese population. Methods: We selected seven top significant associated single nucleotide polymorphisms (SNPs) at 1q22 and 10q23 and conducted a population-based case-control study in a southern Chinese population. Genotypes were determined using MassARRAYTM system (Sequenome, San Diego, CA). Results: Two SNPs at 1q22, rs4072037 and rs4460629, were significantly associated with a reduced risk of GC, best fitting the dominant genetic model. Logistic regression models adjusted for age and sex showed that rs4072037 AG and GG (OR=0.64, P=0.017, compared with AA) and rs4460629 CT and TT (OR=0.54, P=0.0016, compared with TT) significantly reduced the risk of GC. However, no significant results for the five SNPs at 10q23 were obtained in this study. Conclusion: These outcomes indicate that 1q22 is associated with GC susceptibility in this southern Chinese population, while an association for the locus at 10q23 was not confirmed.
Yun-yun Chen,Fen-ping Cui,Fang Gu,Ying-ying Zhang 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.11
In this paper, the feasibility of using a one-temperature model to determine plasma’s temperatureby measuring its refractive index is studied based on the consideration that many plasma flow fieldsare not in local thermodynamic equilibrium (LTE). We compare the refractive indices between theone- and the two-temperature models of an arc plasma, in order to discuss the applicability ofthe one-temperature model in optical diagnosis. We found that the applicability is determined bythe pressure, the temperature ratio between electrons and heavy particles, the probe wavelength,and the chosen optical method’s refractive index measurement precision. Consequently, a commonmodel is deduced to describe the refractive index of plasma whether it be in LTE or not. In a word,this study is crucial to better apply optical methods based on measurements of the refractive indexto diagnose plasma flow fields.
Exploration of Molecular Mechanisms of Diffuse Large B-cell Lymphoma Development Using a Microarray
Zhang, Zong-Xin,Shen, Cui-Fen,Zou, Wei-Hua,Shou, Li-Hong,Zhang, Hui-Ying,Jin, Wen-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Objective: We aimed to identify key genes, pathways and function modules in the development of diffuse large B-cell lymphoma (DLBCL) with microarray data and interaction network analysis. Methods: Microarray data sets for 7 DLBCL samples and 7 normal controls was downloaded from the Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified with Student's t-test. KEGG functional enrichment analysis was performed to uncover their biological functions. Three global networks were established for immune system, signaling molecules and interactions and cancer genes. The DEGs were compared with the networks to observe their distributions and determine important key genes, pathways and modules. Results: A total of 945 DEGs were obtained, 272 up-regulated and 673 down-regulated. KEGG analysis revealed that two groups of pathways were significantly enriched: immune function and signaling molecules and interactions. Following interaction network analysis further confirmed the association of DEGs in immune system, signaling molecules and interactions and cancer genes. Conclusions: Our study could systemically characterize gene expression changes in DLBCL with microarray technology. A range of key genes, pathways and function modules were revealed. Utility in diagnosis and treatment may be expected with further focused research.
Zhang, Jia,Chen, Lin-Ling,Guo, Zi-Fen,Peng, Cui-Ying,Liao, Duan-Fang,Li, Kai Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.6
The potential physiological role and technological application of the premature termination of DNA polymerization through the off-switch of exo+ polymerases were studied using 3' phosphorothioate-modified or unmodified primers with single base mismatch distal to the 3' terminus. With exonuclease-digestible unmodified primers, a gradient premature termination of DNA polymerization was observed when amplified with exo+ polymerases. With 3' allele specific phosphorothioate-modified primers, an efficient off-switch effect occurred in the discrimination of a single nucleotide polymorphism when directly using genomic DNA. Clearly, the off-switch of exo+ polymerases is useful in biomedical research.
( Jia Zhang ),( Lin Ling Chen ),( Zi Fen Guo ),( Cui Ying Peng ),( Duan Fang Liao ),( Kai Li ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.6
The potential physiological role and technological application of the premature termination of DNA polymerization through the off-switch of exo+ polymerases were studied using 3` phosphorothioate-modified or unmodified primers with single base mismatch distal to the 3` terminus. With exonuclease-digestible unmodified primers, a gradient premature termination of DNA polymerization was observed when amplified with exo+ polymerases. With 3` allele specific phosphorothioate-modified primers, an efficient off-switch effect occurred in the discrimination of a single nucleotide polymorphism when directly using genomic DNA. Clearly, the off-switch of exo+polymerases is useful in biomedical research.
만설분,양의,최검,정도,마려,Wan, Xue-Fen,Yang, Yi,Cui, Jian,Zheng, Tao,Ma, Li The Institute of Electronics and Information Engin 2012 전자공학회논문지 Vol.49 No.12
본 논문에서는 중국 현지 염색 기업의 열 센서망에 적용 가능한 원격 가상 현실 제어 플랫폼에서 실행할 수 있는 데이터베이스 액세스를 가상 현실 모델링 언어 (VRML)를 소개하였다. 또한, 3차원 실시간 자료 시각화를 위한 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP)를 소개하였다. 나아가 이와 관련된 스크립트도 다루었을 뿐만 아니라, 열 센서 노드와 센서 영역에서의 움직임을 분석하였다. 이 데이터베이스 프레임워크는 마이크로칩에서 발표한 마이와이$^{TM}$($MiWi^{TM}$)의 표준에 최적화되었다. 이러한 분석 결과를 근거로, 시스템 구성에 따라 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP) 데이터 액세스 프레임워크가 가상 현실 원격 산업 공정 제어 시스템을 위한 하나의 경쟁력 있는 데이터 관리 해결책이 될 수 있음을 확인할 수 있었다. A Virtual Reality Modeling Language (VRML) database access in remote virtual reality control platform for dyeing enterprise $MiWi^{TM}$ thermal sensor network is presented in this paper. The VRML-ASP framework is introduced for 3D real-time data plotting in this application. The activities of thermal sensor nodes and sensor area are analyzed. The database access framework is optimized for $MiWi^{TM}$ wireless sensor networks. The experimental results show that VRML-ASP database access framework could be a reliable and competitive data-manage candidate for targeted virtual reality remote industrial visualization application.
Yang, Li-Min,Li, Xiu-Hua,Bao, Cui-Fen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Osteosarcoma is the most common primary bone malignancy in children and adolescents, and its clinical outcome is poor. We evaluated the response of GSTP1, ERCC1 and ERCC2 to chemotherapy among osteosarcoma patients, and the role of these genes on the prognosis of osteosarcoma. 187 patients with osteosarcoma were administered with methotrexate, cisplatin/adriamycin, actinomycin D, cyclophosphamide, or vincristine treatment. GSTP1, ERCC1 and ERCC2 polymorphism was genotyped by PCR-RFLP assay. The results showed the average survival time of 187 patients were 38.4 months. 97 patients showed response to neoadjuvant chemotherapy. The GSTP1 Val and ERCC2 A/A genotypes had significantly higher rates of response to chemotherapy, with adjusted OR (95% CI) of 2.19 (1.15-6.21) and 2.88 (1.14-13.25). Individuals with ERCC2 A/A genotype were likely to have a lower risk of death from oseosarcoma, and the adjusted HR was 0.32 (0.13-0.95). Our study indicated test of GSTP1 and ERCC2 Lys751Gln polymorphisms might be a candidate pharmacogenomic factors to be explored in the future to identify the osteosarcoma patients who might benefit from chemotherapy.