Chlorhexidine gel associated with papain in pulp tissue dissolution

Couto De Oliveira, Gabriel,Ferraz, Caio Souza,Andrade Junior, Carlos Vieira,Pithon, Matheus Melo The Korean Academy of Conservative Dentistry 2013 Restorative Dentistry & Endodontics Vol.38 No.4

Objectives: This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution. Materials and Methods: Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min. Results: The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively. Conclusions: The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite.

Formation of ghost images due to metal objects on the surface of the patient’s face: A pictorial essay

Bárbara Couto Ramos,Bruna Raquel da Silva Izar,Jéssica Lourdes Costa Pereira,Priscilla Sena Souza,Claudia Scigliano Valerio,Fabrício Mesquita Tuji,Flávio Ricardo Manzi 대한영상치의학회 2016 Imaging Science in Dentistry Vol.46 No.1

Panoramic radiographs are a relatively simple technique that is commonly used in all dental specialties. In panoramic radiographs, in addition to the formation of real images of metal objects, ghost images may also form, and these ghost images can hinder an accurate diagnosis and interfere with the accuracy of radiology reports. Dentists must understand the formation of these images in order to avoid making incorrect radiographic diagnoses. Therefore, the present study sought to present a study of the formation of panoramic radiograph ghost images caused by metal objects in the head and neck region of a dry skull, as well as to report a clinical case in order to warn dentists about ghost images and to raise awareness thereof. An understanding of the principles of the formation of ghost images in panoramic radiographs helps prevent incorrect diagnoses.

Intradermal Tests for Diagnosis of Drug Allergy are not Affected by a Topical Anesthetic Patch

Mariana Couto,Diana Silva,Ana Ferreira,Josefina R. Cernadas 대한천식알레르기학회 2014 Allergy, Asthma & Immunology Research Vol.6 No.5

The use of topical anesthesia to perform intradermal tests (IDTs) for drug allergy diagnosis was never investigated. We aimed to determine the effectsof a topical anesthetic patch containing prilocaine-lidocaine on wheal size of IDT with drugs. Patients who had positive IDT as part of their investigationprocess of suspected drug hypersensitivity were selected. IDT were performed according to guidelines. Anesthetic patch (AP) wasplaced and the same prior positive IDT, as well as positive histamine skin prick test (SPT) and negative (saline IDT) controls, were performed in theanesthetized area. Patients with negative IDT were also included to check for false positives with AP. Increase in wheals after 20 minutes both withand without AP was recorded and compared. 45 IDT were performed (36 patients), of which 37 have been previously positive (14 antibiotics, 10 generalanesthetics, 6 non-steroidal anti-inflammatory drugs, 3 iodinated contrasts, 3 anti-Hi-histamines and 1 ranitidine). Mean histamine SPT sizewithout the AP was 4.7 mm [95%CI (4.4-5.1)], and 4.6 mm [95%CI (4.2-5.0)] with anesthesia. Mean wheal increase in IDT for drugs without the anesthesiawas 4.5 mm [95%CI (3.3-5.7)] and with anesthesia was 4.3 mm [95%CI (2.8-5.8)]. No statistical significant differences were observed betweenskin tests with or without AP for histamine SPT (P=0.089), IDT with saline (P=0.750), and IDT with drugs (P=0.995). None of the patientswith negative IDT showed positivity with the AP, or vice-versa. The use of an AP containing prilocaine-lidocaine does not interfere with IDT to diagnosedrug allergy, and no false positive tests were found.

Enhancement of nitrate electroreduction using BDD anode and metal modified carbon fiber cathode

A.B. Couto,S.S. Oishi,N.G. Ferreira 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.39 No.-

Silver and copper were electrodeposited on carbon fiber (CF) with heat treatment temperatures of1000 and 2000 8C. Cu/CF1000 and Ag/CF1000 presented more uniform particles with higher densitiesthan those for CF2000. This behavior was attributed to electrostatic force between Cu2+/Ag+ and C55Ofunctional group present on CF1000 surface. Nitrate electroreduction was carried out using Ag/CF or Cu/CF cathode and BDD anode. The rate-determining step of nitrate reduction was its transformation intonitrite ions. The best performance for electrochemical nitrate reduction was achieved using Cu/CF1000resulting in 100% of nitrate reduction not to mention its low by-product formation.

Macrophages in cardiac repair: Environmental cues and therapeutic strategies

Geoffrey de Couto 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Mammals, in contrast to urodeles and teleost fish, lose the ability to regenerate their hearts soon after birth. Central to this regenerative response are cardiac macrophages, which comprise a heterogeneous population of cells with origins from the yolk sac, fetal liver, and bone marrow. These cardiac macrophages maintain residency in the myocardium through local proliferation and partial replacement over time by circulating monocytes. The intrinsic plasticity of cardiac macrophages in the adult heart promotes dynamic phenotypic changes in response to environmental cues, which may either protect against injury or promote maladaptive remodeling. Thus, therapeutic strategies promoting myocardial repair are warranted. Adult stromal cell-derived exosomes have shown therapeutic promise by skewing macrophages toward a cardioprotective phenotype. While several key exosomal non-coding RNA have been identified, additional factors responsible for cardiomyocyte proliferation remain to be elucidated. Here I review cardiac macrophages in development and following injury, unravel environmental cues modulating macrophage activation, and assess novel approaches for targeted delivery.

Formation of ghost images due to metal objects on the surface of the patient's face: A pictorial essay

Ramos, Barbara Couto,da Silva Izar, Bruna Raquel,Pereira, Jessica Lourdes Costa,Souza, Priscilla Sena,Valerio, Claudia Scigliano,Tuji, Fabricio Mesquita,Manzi, Flavio Ricardo Korean Academy of Oral and Maxillofacial Radiology 2016 Imaging Science in Dentistry Vol.46 No.1

Panoramic radiographs are a relatively simple technique that is commonly used in all dental specialties. In panoramic radiographs, in addition to the formation of real images of metal objects, ghost images may also form, and these ghost images can hinder an accurate diagnosis and interfere with the accuracy of radiology reports. Dentists must understand the formation of these images in order to avoid making incorrect radiographic diagnoses. Therefore, the present study sought to present a study of the formation of panoramic radiograph ghost images caused by metal objects in the head and neck region of a dry skull, as well as to report a clinical case in order to warn dentists about ghost images and to raise awareness thereof. An understanding of the principles of the formation of ghost images in panoramic radiographs helps prevent incorrect diagnoses.

Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

da Silva Danilo Couto,Vaz Leonardo Gomes,Tavares Warley Luciano Fonseca,Vieira Leda Quercia,de Oliveira Ricardo Reis,Sobrinho Antônio Paulino Ribeiro 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.3

Objectives This study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation. Materials and Methods Cell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not. Results After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS. Conclusions Our results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.

Improving accessibility and distinction between negative results in biomedical relation extraction

Sousa, Diana,Lamurias, Andre,Couto, Francisco M. Korea Genome Organization 2020 Genomics & informatics Vol.18 No.2

Accessible negative results are relevant for researchers and clinicians not only to limit their search space but also to prevent the costly re-exploration of research hypotheses. However, most biomedical relation extraction datasets do not seek to distinguish between a false and a negative relation among two biomedical entities. Furthermore, datasets created using distant supervision techniques also have some false negative relations that constitute undocumented/ unknown relations (missing from a knowledge base). We propose to improve the distinction between these concepts, by revising a subset of the relations marked as false on the phenotype-gene relations corpus and give the first steps to automatically distinguish between the false (F), negative (N), and unknown (U) results. Our work resulted in a sample of 127 manually annotated FNU relations and a weighted-F1 of 0.5609 for their automatic distinction. This work was developed during the 6th Biomedical Linked Annotation Hackathon (BLAH6).

Chlorhexidine gel associated with papain in pulp tissue dissolution

Matheus Melo Pithon,Gabriel Couto De Oliveira,Caio Souza Ferraz,Carlos Vieira Andrade Júnior 대한치과보존학회 2013 Restorative Dentistry & Endodontics Vol.38 No.4

Objectives This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution. Materials and Methods Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min. Results The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively. Conclusions The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite. Objectives This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution. Materials and Methods Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min. Results The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively. Conclusions The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite.

Uptake of Reactive Black 5 by pumice and walnut activated carbon: Chemistry and adsorption mechanisms

Behzad Heibati,Susana Rodriguez-Couto,Abdeltif Amrane,Mohd. Rafatullah,Alaa Hawari,Mohammad A. Al-Ghouti 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

The potential of using pumice and walnut wood activated carbon as low-cost adsorbents for the removal of the diazo dye Reactive Black 5 (RB5) from aqueous solutions was investigated. The Langmuir isotherm fit to the data specified the presence of two different natures of adsorption sites with different binding energies on the AC-W surface. Kinetic modelling showed that the adsorption behaviour and mechanism of RB5 for both adsorbents is believed to happen via surface adsorption followed by diffusion into the pores of the AC-W and pumice. The main adsorption mechanisms are hydrogen bonding, electrostatic bonding and n–π interactions.