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Modulation of nuclear PI-PLCbeta1 during cell differentiation
Cocco, L.,Manzoli, L.,Faenza, I.,Ramazzotti, G.,Yang, Y.R.,McCubrey, J.A.,Suh, P.G.,Follo, M.Y. Elsevier 2016 Advances in biological regulation Vol.60 No.-
PI-PLCbeta1 plays an important role in cell differentiation, and particularly in myogenesis, osteogenesis and hematopoiesis. Indeed, the increase of PI-PLCbeta1, along with Cyclin D3, has been detected in C2C12 mouse myoblasts induced to differentiate, as well as in human cells obtained from myotonic dystrophy. Also in the case of osteogenic differentiation there is a specific induction of PI-PLCbeta1, but in this case the role of PI-PLCbeta1 seems to be independent from Cyclin D3, so that a different mechanism could be involved. As for the hematopoietic system, PI-PLCbeta1 has a peculiar behavior: it increases during myeloid differentiation and decreases during erythroid differentiation, thus confirming the role of PI-PLCbeta1 as a modulator of hematopoiesis.
Phosphoinositide-specific phospholipase C in health and disease
Cocco, Lucio,Follo, Matilde Y.,Manzoli, Lucia,Suh, Pann-Ghill American Society for Biochemistry and Molecular Bi 2015 Journal of lipid research Vol.56 No.10
<P>Phospholipases are widely occurring and can be found in several different organisms, including bacteria, yeast, plants, animals, and viruses. Phospholipase C (PLC) is a class of phospholipases that cleaves phospholipids on the diacylglycerol (DAG) side of the phosphodiester bond producing DAGs and phosphomonoesters. Among PLCs, phosphoinositide-specific PLC (PI-PLC) constitutes an important step in the inositide signaling pathways. The structures of PI-PLC isozymes show conserved domains as well as regulatory specific domains. This is important, as most PI-PLCs share a common mechanism, but each of them has a peculiar role and can have a specific cell distribution that is linked to a specific function. More importantly, the regulation of PLC isozymes is fundamental in health and disease, as there are several PLC-dependent molecular mechanisms that are associated with the activation or inhibition of important physiopathological processes. Moreover, PI-PLC alternative splicing variants can play important roles in complex signaling networks, not only in cancer but also in other diseases. That is why PI-PLC isozymes are now considered as important molecules that are essential for better understanding the molecular mechanisms underlying both physiology and pathogenesis, and are also potential molecular targets useful for the development of innovative therapeutic strategies.</P>
Development of a nonlinear seismic response capacity spectrum method for intake towers of dams
Cocco, Leonardo,Suarez, Luis E.,Matheu, Enrique E. Techno-Press 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.36 No.3
The seismic-induced failure of a dam could have catastrophic consequences associated with the sudden release of the impounded reservoir. Depending on the severity of the seismic hazard, the characteristics and size of the dam-reservoir system, preventing such a failure scenario could be a problem of critical importance. In many cases, the release of water is controlled through a reinforced-concrete intake tower. This paper describes the application of a static nonlinear procedure known as the Capacity Spectrum Method (CSM) to evaluate the structural integrity of intake towers subject to seismic ground motion. Three variants of the CSM are considered: a multimodal pushover scheme, which uses the idea proposed by Chopra and Goel (2002); an adaptive pushover variant, in which the change in the stiffness of the structure is considered; and a combination of both approaches. The effects caused by the water surrounding the intake tower, as well as any water contained inside the hollow structure, are accounted for by added hydrodynamic masses. A typical structure is used as a case study, and the accuracy of the CSM analyses is assessed with time history analyses performed using commercial and structural analysis programs developed in Matlab.
Development of a nonlinear seismic response capacity spectrum method for intake towers of dams
Leonardo Cocco,Luis E. Suárez,Enrique E. Matheu 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.36 No.3
The seismic-induced failure of a dam could have catastrophic consequences associated with the sudden release of the impounded reservoir. Depending on the severity of the seismic hazard, the characteristics and size of the dam-reservoir system, preventing such a failure scenario could be a problem of critical importance. In many cases, the release of water is controlled through a reinforced-concrete intake tower. This paper describes the application of a static nonlinear procedure known as the Capacity Spectrum Method (CSM) to evaluate the structural integrity of intake towers subject to seismic ground motion. Three variants of the CSM are considered: a multimodal pushover scheme, which uses the idea proposed by Chopra and Goel (2002); an adaptive pushover variant, in which the change in the stiffness of the structure is considered; and a combination of both approaches. The effects caused by the water surrounding the intake tower, as well as any water contained inside the hollow structure, are accounted for by added hydrodynamic masses. A typical structure is used as a case study, and the accuracy of the CSM analyses is assessed with time history analyses performed using commercial and structural analysis programs developed in Matlab.
Deise Jaqueline Stroher,Micaela Federizzi de Oliveira,Patrıcia Martinez-Oliveira,Bruna Cocco Pilar,Marcia Denise Pavanelo Cattelan,Eliseu Rodrigues,Kalyne Bertolin,Paulo Bayard Dias Gonc¸alves,Jacquel 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.7
Obesity reaches an epidemic level worldwide, and this condition is associated with chronic low-grade inflammation and secondary comorbidities, largely driven by global changes in lifestyle and diet. Various dietary approaches are proposed for the obesity treatment and its associated metabolic disorders. Good taste, antioxidant functions, and vitamins have been attributed to virgin coconut oil (VCO). However, VCO contains a large amount of saturated fatty acids, and the consumption of this fat is associated with a number of secondary diseases. We evaluate the effects of VCO supplementation on biochemical, inflammatory, and oxidative stress parameters in rats fed with high-fat diet (HFD). After feeding with HFD for 12 weeks, the animals were supplemented with VCO for 30 days. HFD+VCO group increased in diet intake, weight gain, low-density lipoprotein cholesterol level, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. These findings were accompanied by increased in hepatic lipid profile and fat deposition in the liver. Adipocyte hypertrophy was observed in the HFD+VCO group, which was associated with elevated expression of tumor necrosis factor alpha (TNF-α) in adipose tissue. These results revealed that VCO associated with HFD induced important metabolic alterations, adipose inflammation, and hepatic lipid accumulation in rats.
PLCγ1: Potential arbitrator of cancer progression
Jang, Hyun-Jun,Suh, Pann-Ghill,Lee, Yu Jin,Shin, Kyeong Jin,Cocco, Lucio,Chae, Young Chan Elsevier 2018 Advances in biological regulation Vol.67 No.-
<P><B>Abstract</B></P> <P>Phospholipase C (PLC) is an essential mediator of cellular signaling. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP<SUB>3</SUB>) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca<SUP>2+</SUP>) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. PLCγ1, one of the primary subtypes of PLC, is directly activated by membrane receptors, including receptor tyrosine kinases (RTKs), and adhesion receptors such as integrin. PLCγ1 mediates signaling through direct interactions with other signaling molecules via SH domains, as well as its lipase activity. PLCγ1 is frequently enriched and mutated in various cancers, and is involved in the processes of tumorigenesis, including proliferation, migration, and invasion. Although many studies have suggested that PLCγ functions in cell mobility rather than proliferation in cancer, questions remain as to whether PLCγ regulates mitogenesis and whether PLCγ promotes or inhibits proliferation. Moreover, how PLCγ regulates cancer-associated cellular processes and the interplay among other proteins involved in cancer progression have yet to be fully elucidated. In this review, we discuss the current understanding of the role of PLCγ1 in cancer mobility and proliferation.</P>
Patrícia Martinez-Oliveira,Luísa Zuravski,Micaela Federizzi de Oliveira,Deise Jaqueline Ströher,Ritiéle Pinto Coelho,Bruna Cocco Pilar,Luís Flávio Souza de Oliveira,Jacqueline da Costa Escobar Piccoli 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.6
Yacon is an Andean plant that has been used in folk medicine for its medicinal properties. The beneficial effects of this plant are possibly due to the high content of phenolic compounds present in its leaves and roots. This study evaluated the in vitro toxicity of the hydroalcoholic extract of leaves and roots from yacon (1, 10, 50, and 100 μg/mL) through cell viability tests, genotoxic and mutagenic activity in leukocytes culture cells; and cytotoxicity and apoptosis cell death (1, 10, 50, 100, and 500 μg/mL) in cell line originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called 3T3 protocol “3-day transfer, inoculum 3 × 105 cells” (3T3 cell line). No mutagenic and cytotoxic activities were observed in leukocyte cultures. Cytotoxic activity was evidenced in the highest concentrations of yacon leaf extract (50 and 100 μg/mL), whereas all concentrations tested with yacon leaf extract there was induction for apoptosis in the 3T3 cells. Genotoxic potential was observed only at higher doses of leaf (50 and 100 μg/mL) and root (100 μg/mL) extract. These results suggest that yacon leaf at high concentrations may present toxic potential showing concentration-dependent behavior; however, in vivo studies should be performed to validate these results.