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Nogueira, Daniele Rubert,Rolim, Clarice M. Bueno,Farooqi, Ammad Ahmad Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
Nanotechnology is an emerging field with many promising applications in drug delivery systems. Because of outstanding developments in this field, rapidly increasing research is directed to the development of nanocarriers that may enhance the availability of drugs to the target sites. Substantial fraction of information has been added into the existing scientific literature focusing on the fact that nanoparticles usually generate reactive oxygen species to a greater extent than micro-sized particles. It is worth mentioning that oxidative stress regulates an array of cell signaling cascades that resulted in cancer cell damage. Accumulating experimental evidence over the years has shown that wide-ranging biological mechanisms are triggered by these NPs in cultured cells due to the unique properties of engineered nanoparticles. In this review, we have attempted to provide an overview of the signaling cascades that are activated by oxidative stress in cancer cells in response to different kinds of nanomaterials, including quantum dots, metallic and polymeric nanoparticles.
Podcasting and Politics in Singapore: An Experimental Study of Medium Effects
Skoric, Marko M.,Sim, Clarice,Juan, Han Teck,Fang, Pam World Association for Triple Helix and Future Stra 2009 Journal of Contemporary Eastern Asia Vol.8 No.2
A ban on political podcasting during the General Elections 2006 in Singapore was justified by the Singaporean government on the grounds that the new medium had a greater power to influence voters than traditional modes of political discourse. A between-subjects controlled experiment was conducted to test whether podcasts of political speeches had a greater power to influence voters' evaluations of political candidates and likelihood of voting for them than online text-based transcripts of the same speeches. The study also examined whether mere exposure to political speeches online, irrespective of the modality, had an effect on voters' more general political preferences, i.e. the likelihood of support and voting for the opposition. The findings suggest that political podcasts were no more persuasive than text-based websites and that the effects on political preferences, if any, were likely due the exposure to political content online, not because of the nature of the medium. The implications of the findings are discussed.
Kim-Hung Huynh,홍명기,Clarice Lee,Huyen-Thi Tran,이상희,안예진,차선신,강린우 한국미생물학회 2015 The journal of microbiology Vol.53 No.11
Acinetobacter baumannii, which is emerging as a multidrugresistant nosocomial pathogen, causes a number of diseases, including pneumonia, bacteremia, meningitis, and skin infections. With ATP hydrolysis, the D-alanine-D-alanine ligase (DDL) catalyzes the synthesis of D-alanyl-D-alanine, which is an essential component of bacterial peptidoglycan. In this study, we determined the crystal structure of DDL from A. baumannii (AbDDL) at a resolution of 2.2 Å. The asymmetric unit contained six protomers of AbDDL. Five protomers had a closed conformation in the central domain, while one protomer had an open conformation in the central domain. The central domain with an open conformation did not interact with crystallographic symmetry-related protomers and the conformational change of the central domain was not due to crystal packing. The central domain of AbDDL can have an ensemble of the open and closed conformations before the binding of substrate ATP. The conformational change of the central domain is important for the catalytic activity and the detail information will be useful for the development of inhibitors against AbDDL and putative antibacterial agents against A. baumannii. The AbDDL structure was compared with that of other DDLs that were in complex with potent inhibitors and the catalytic activity of AbDDL was confirmed using enzyme kinetics assays.
Luquet Martin,Moulin Clarice,Cortesero Anne-Marie,Anton Sylvia,Jaloux Bruno 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
Experience of host-associated olfactory stimuli during development affects subsequent foraging decisions in many parasitoids, leading to host fidelity. We have recently shown that odours emitted by an alien host-plant complex (HPC) may affect this learning process. However, the consequences of this olfactory experience on parasitoid host foraging decisions is unknown. Here, we investigated if olfactory preferences induced by ex perience might drive parasitoid HPC choice and oviposition decisions. We presented two HPCs in dual choice experiments to Aphidius ervi females that had been reared on each HPC, either in a simple (one HPC) or complex (two HPCs) olfactory environment. HPC choice, time before landing on a HPC and number of aphid attacks were recorded. Early experience had contrasting effects on each of these parameters. Regardless of their origin, parasitoids did not land preferentially on any HPC, but they more frequently attacked one of the two hosts (Acyrthosiphon pisum) once they had landed on its HPC. Females emerging from the A. pisum/faba bean HPC attacked more aphids, regardless of the host species. Finally, the olfactory complexity of the environment had inconsistent effects on foraging decisions. These observations, contrasting with previous results obtained in olfactometer assays, indicate that olfactory preferences induced in early stages are not sufficient to predict parasitoid oviposition decisions. Moreover, the integration of multiple signals at several scales might generate different foraging patterns at each step of the host selection process.
da Silva Suéllen Pedrosa,da Costa Clarice Barbosa Lucena,de Freitas Anderson Felipe Soares,da Silva José Dayvid Ferreira,Costa Wêndeo Kennedy,da Silva Wênio Sandoval Filho Lima,Machado Janaina Carla B 한국독성학회 2023 Toxicological Research Vol.39 No.2
The present study aimed to evaluate saline extracts from the leaves (LE) and stem (SE) of Portulaca elatior in relation to their phytochemical composition and photoprotective and antioxidant effects, as well as to evaluate the toxicity of the leaf extract. The extracts were characterized for protein concentration and phenol and flavonoid contents, as well as for thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) profiles. Total antioxidant capacity and DPPH and ABTS+ scavenging activities were determined. In the photoprotective activity assay, the sun protection factor (SPF) was calculated. The toxicity evaluation of LE included in vitro hemolytic assay and in vivo oral and dermal acute toxicity assays in Swiss mice. LE showed the highest protein, phenol, and flavonoid (8.79 mg/mL, 323.46 mg GAE/g, and 101.96 QE/g, respectively). TLC revealed the presence of flavonoids, reducing sugars, terpenes, and steroids in both extracts. In HPLC profiles, LE contained flavonoids, while SE contained flavonoids and ellagic tannins. The antioxidant activity assays showed the lowest IC50 values (34.15–413.3 μg/mL) for LE, which presented relevant SPF (> 6) at 50 and 100 μg/mL. LE demonstrated low hemolytic capacity, and no signs of intoxication were observed in mice treated orally or topically at 1000 mg/kg. However, at 2000 mg/kg, an increase in the mean corpuscular volume of erythrocytes and a reduction in lymphocytes were observed; animals treated topically with 2000 mg/kg displayed scratching behavior during the first hour of observation and showed edema and erythema that regressed after six days. In conclusion, LE did not present acute oral or dermal toxicity in Swiss mice at a dose of 1000 mg/kg and showed slight toxicity in animals treated with 2000 mg/kg.
Skoric, Marko M.,Ji, Pan,Fu, Wayne Wei-Jen,Sim, Clarice Chwei Lin,Park, Yongjin World Association for Triple Helix and Future Stra 2015 Journal of Contemporary Eastern Asia Vol.14 No.1
This study examines how different uses of social network sites (SNS) and mobile phones (MP) to communicate with friends and business associates are related to the acquisition of job-related information, job mobility, and entrepreneurial intentions, using social capital as its main theoretical lens. To this end, a nationally representative, random digit dialing (RDD) survey was conducted in Singapore. Path analyses show that SNS interactions with friends are positively related to both bonding and bridging social capital. The former is linked with greater job mobility, the latter with entrepreneurship, and both are associated with more job-related information. SNS interactions with business contacts are directly positively related to job-related information and entrepreneurship. For mobile phones, interactions with friends are positively related to social capital, job information and entrepreneurship. Professional networking is associated with more bridging social capital, job information and job mobility. Bonding capital is found to be linked with greater job mobility, while bridging capital has a positive relationship with both entrepreneurship and job mobility.
Roberta Gomes Coelho,Neli Kika Honda,Maria do Carmo Vieira,Rosenei Louzada Brum,Fernando Roge´rio Pavan,Clarice Queico Fujimura Leite,Claudia Andre´a Lima Cardoso 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
Bromelia balansae (Family Bromeliaceae) is a medicinal plant commonly used in the central region of Brazil as a cough syrup and also eaten roasted. The methanolic extract of ripe fruits was analyzed by chromatographic methods and spectrometrically. Four glycoside flavonols were isolated: kaempferol-3-O-α-l-rhamnopyranoside (1), kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (2), quercetin-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (3), and kaempferol 3,7-di-O-α-l-rhamnopyranoside (4). The resazurin microtiter assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis. The results showed a moderate activity of the methanolic extract with a minimal inhibitory concentration of 128μg/mL. Antioxidant activity was evaluated as free radical scavenging capacity and inhibition of peroxidation. Free radical scavenging capacity was assessed by measuring the scavenging activity of methanolic extract and methanolic fraction on 2,2-diphenyl-1-picrylhydrazyl radical. The methanolic extract showed low values of antioxidant activities, whereas the methanolic fraction exhibited free radical scavenging activity ranging from 20.2% to 91.1%, and the inhibition of peroxidation values ranging from 5.6% to 27.5%. This is the first chemical study reported in the literature about this species.
권순재,Allan F. Brown,Jinguo Hu,Rebecca McGee,Chasity Watt,Ted Kisha,Gail Timmerman-Vaughan,Michael Grusak,Kevin E. McPhee,Clarice J. Coyne 한국유전학회 2012 Genes & Genomics Vol.34 No.3
Genetic diversity, population structure and genome-wide marker-trait association analysis was conducted for the USDA pea (Pisum sativum L.) core collection. The core collection contained 285 accessions with diverse phenotypes and geographic origins. The 137 DNA markers included 102 polymorphic fragments amplified by 15 microsatellite primer pairs,36 RAPD loci and one SCAR (sequence characterized amplified region) marker. The 49 phenotypic traits fall into the categories of seed macro- and micro-nutrients, disease resistance,agronomic traits and seed characteristics. Genetic diversity,population structure and marker-trait association were analyzed with the software packages PowerMarker, STUCTURE and TASSEL, respectively. A great amount of variation was revealed by the DNA markers at the molecular level. Identified were three sub-populations that constituted 56.1%, 13.0% and 30.9%, respectively, of the USDA Pisum core collection. The first sub-population is comprised of all cultivated pea varieties and landraces; the second of wild P. sativum ssp. elatius and abyssinicum and the accessions from the Asian highland (Afghanistan, India, Pakistan, China and Nepal); while the third is an admixture containing alleles from the first and second sub-populations. This structure was achieved using a stringent cutoff point of 15% admixture (q-value 85%) of the collection. Significant marker-trait associations were identified among certain markers with eight mineral nutrient concentrations in seed and other important phenotypic traits. Fifteen pairs of associations were at the significant levels of P≤ 0.01when tested using the three statistical models. These markers will be useful in marker-assisted selection to breed pea cultivars with desirable agronomic traits and end-user qualities.