Agrobacterium tumefaciens의 Ti plasmid 재조합에 관한 연구

주충노,이희봉,김영명 연세대학교 자연과학연구소 1987 學術論文集 Vol.19 No.-

Crown gall의 병원체로 알려진 Agrobacterium tumefaciens가 함유하고 있는 Tumor inducing(Ti) plasmid가 식물 hormone과 opine의 합성원인이라는 것이 밝혀지고 식물 세포의 crown gall에는 Ti plasmid가 존재하며 Ti plasmid의 일부가 식물세포의 핵 genome에 삽입되어 형질 변화가 일어남이 보고 되었고 Ti plasmid의 식물체로의 gene도입 vector로서의 연구가 최근 활발히 진행되고 있으며 현재 PLGV vector와 PMON vector 등이 개발되고 있으나 조작이 복잡하고 아직은 초보적 단계인 것으로 알려져 있다. 본 연구에서는 원하는 식물 gene을 식물체에 도입하는 유용한 vector개발의 초보단계로서 Ti plasmid의 T-DNA의 일부인 nos gene을 분리하여 pBR322와의 재조합을 기도한 것이다. pBR 322를 포함하는 E. coli로 부터 Horwitz(1979)의 방법으로 pBR 322를 분리하였고 A. tumefaciens C58에서 Kado(1979)의 방법으로 Ti plasmid를 분리한 후 Ti plasmid 내의 nos gene을 분리하였다. 분리된 pBR 322를 Hind Ⅲ로 절단한 후 CIP로 처리하고 T_4 DNA ligase를 이용하여 Hind Ⅲ로 처리한 nos gene fragment와 재조합하였다. 이와같이 하여 얻은 recombinant DNA로 인한 E. coli HB101의 형질 변화를 Mandel과 Higa의 방법에 따라 조사한 결과 pBR 322의 tetracyclin gene 사이에 nos gene이 삽입된 것으로 확인되었다. 즉 ampicillin 배지에서는 92개의 colony가 형성되었는데 그중 59개는 tetracyclin 배지에서 colony를 형성하지 못하였다. 이러한 tetracyclin 감수성이면서 ampicillin 저항성인 colony를 배양하여 얻은 recombinant plasmid를 전기이동법으로 분리한 결과 예상대로 크기가 7.5kb 정도였고 Hind Ⅲ로 절단하였을 때 2개의 band가 확인되었다. 본 연구에서 얻은 nos gene-pBR 322 hybrid plasmid는 원하는 식물 gene을 이것에 삽입하여 A. tumefaciens 내에서 homologous recombination을 행한 후 식물체에 감염시켜 원하는 gene의 발현여부를 검출하는데 이용될 것으로 기대된다. It is mow realized that the tumor-inducing(Ti) plasmid in Agrobacterium tumefaciens is responsible for the induction of crown gall tumors in dicotyledonous plants and it has been demonstrated that a segment of the Ti plasmid, so called T-DNA, is stably integrated into and expressed in the genome of transformed plant cells. Recently, the use of A. tumefaciens Ti plasmid as a vector to introduce a foreign gene has been intensively studied and several vectors such as PLGV vector and PMON vector has been reported. However, their practical use is still at a primitive stage. It was attempted in the present study, therefore, to conduct the recombination of nos gene of T-DNA from A. tumefaciens Ti plasmid and E. coli pBR 322 as a vector it introduce foreign plant gene into dicotyledon plant cells. pBR 322 was isolated from E. coli containing pBR 322 accdrding to Horowitz(1979) and nos gene of T-DNA in Ti plasmid from A. tumefaciens C58 was obtained according to Kado(1979). The isolated pBR 322 was cut using Hind Ⅲ followed by the addition of CIP·pBR 322 fragments and Hind Ⅲ treated nos gene fragments were than recombined using T_4 DNA ligase and the E. coli cells were then transformed by recombinant DNA according to Mandel and Higa(1970). It was confirmed that nos gene was inserted into tetracyclin gene of pBR 322 by the finding that when the transformed cells were incubated in the ampicillin medium, 92 colonies were formed, in which 59 were tetracyclin sensitive. The above tetracyclin sensitive but ampicillin resistant cells were then cultured and the recombinant pBR-nos gene was isolated and subjected to electrophoresis. It was found that size of the recombinant DNA was approximately 7.5 Kb as expected and two bands were appeared on electrophoretogram when the recombinant DNA was treated with Hind Ⅲ. It might be possible to insert the foreign plant genes to the pBR-nos gene hybrid plasmid obtained in the present study and the resultant DNA could be then homolgously recombined with Ti plasmid of A. tumefaciens and the final recombined Ti plasmid could be used for the introduction of the foreign plant genes into dicotyledon plant cells.

現代 産業技術의 特質과 科學敎育

朱忠魯 연세대학교 교육대학원 1977 연세교육과학 Vol.11 No.-

We are all living in the age of science and it is now well recognized that politics, economic prosperity and civilization of our society anti even philosophical problems cannot be dealt with without considering science and technology today. It is also true that science and technology became an indispensale strategic tool for the rapid development of society, particularly for the developing country like Korea. Therefore, science education and its development became one of the serious facing issues of this country. Science of today is growing rapidly and it is one of the characteristics of modern science that it is finely dividing into a number of specialized fields but on the other hand they are going to group into several catagories. Furthermore, industrial technology is so intimately interlinked to science that no industrial development can be made without the background of science and scientific research. Actually science today acts a leading role in industry and few people would venture to assert that the prosperity and industrial development of any society can expect otherwise the rational science education and scientific research being carried out steadily. Science today occupies the heart of modern industry and it is rapidly spreading over industry through scientific production and administration. This rejects the social significance of modern Science. Historical events tell us that well organized and planned policy of science and technology did catalytic role unexceptionally for the prosperity of society in the past. Furthermore, it should he reminded that new technologies were brought by scientific findings and it is extremly dangerous to consider science and technology separately. It cannot he overestimated to emphasize the signifance of fundamental science for industrial development and prosperity of society. We know that science and technology have been developed before industrial prosperities were made, and well organized science education and policies were prepared before the development of science and technology. It is clear therefore that improvement of science education, both teaching contents and methods, must be urgently required to meet the development of industry and prosperity of thin society. There is no doubt that the purpose of science education today is not any more to teach systematic scientific knowledge as before. Rather I think it is necessary to teach fundumental concepts governing today's science and technology and scientific methods so that they may handle their own problems in the future and enjoy their lives rationally in the highly developed scientific society. Accordingly the content of the text of science education must be carefully selected to meet their lives in the future. It is realized that modern science governing today's industry would be consisted of nuclear physics, electronics, polymer chemistry and molecular biology. Baaed on the above fields of science, what it is called universe development has been initiated. From the above considerations, it is suggested that we all, educators, scientists as well as industrial people and government officials have to pay their extreme attention to set up rational programs for science education and scientific research in the very near future, and hoped the programs being carried out properly and steadily for the prosperity of this country.

q진 LCZ 수열군의 일반화된 확장 생성 방법

정정수(Jung-Soo Chung),김영식(Young-Sik Kim),장지웅(Ji-Woong Jang),노종선(Jong-Seon No),정하봉(Ha-Bong Chung) 한국통신학회 2008 韓國通信學會論文誌 Vol.33 No.11C

[1]에서 LCZ 수열군의 2배 확장을 제안하였다. 본 논문에서는 [1]에서의 2배 확장방법을 일반화하는 새로운 확장방법을 제안한다. 이 생성방법을 사용하면 인수가 (N,M,L,ε)인 q진 LCZ 수열군은 인수가(pN,pM,p「(L+1)/p」-,pε)인 q진 LCZ 수열군이 된다. 이 때, p는 소수이고 p는 q의 약수다. 특히 L≡p-1modp 일 때, 확장된 q진 LCZ 수열군의 인수는 (pN,pM,L,pε)이 된다. In this paper, a new extending method of q-ary low correlation zone(LCZ) sequence sets is proposed, which is a generalization of binary LCZ sequence set by Kim, Jang, No, and Chung. Using this method, q-ary LCZ sequence set with parameters (N,M,L,ε) is extended as a q-ary LCZ sequence set with parameters (pN,pM,p「(L+1)/p」-1,pε), where p is prime and p|q.

다운 증후군과 동반된 그레이브스병 1예

노혜정,신진호,김하영,김용성,백승훈,박병현,조정구 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

麻砂骨材에 對한 試驗的 硏究

鄭魯益,吳仁煥 釜山工業大學校 1969 論文集 Vol.9 No.-

광전도성 N-알킬카르바졸 폴리머의 합성에 관한 연구

정평진,박노훈 단국대학교 1998 論文集 Vol.33 No.-

The N­alkylcarbazole polymers were synthesized by cationic polymerization. These polymerizations were reacted under different condition for 3hrs or 24 hrs. These reactions between N­unsaturatedalkylcarbazole monomers and boron trifluoride diethyl etherate(BF_3ㆍEt_2O) were conducted in toluene at ice water bath under N_2 atmosphere or in benzene at room temperature under vacuum atmosphere. At htis time the starting materials were monomer 1.0g and initiator 1.42㎎, respectively. All of these compounds of substrates and products were characterized by melting point. FT­IR and ^1H­NMR spectroscopy.

中心壓軸力을 받는 高强度 鐵筋콘크리트 기둥의 耐力과 延性에 關한 실험적 硏究

곽노현,이영호,김남주,이경종,정헌수 중앙대학교 기술과학연구소 1997 기술과학연구소 논문집 Vol.27 No.-

A study on the behavior of short reinforced concrete columns was experimently described. Twenty-four concrete columns, with 20cm square and 60cm height, containing 8 longitudinal steel bars and different the amount of ties, spacing and concrete strengths, were subjected to concentric load by failure. Test results indicated that 1) It is incompatible in high-strength concrete to arrange stirrups to the AIK code of D/2 throughout the example of 10cm stirrup spacing following AIK codes. 2) As the amount of stirrup increased, the strength and ductily of reinforced concrete column improved. 3) As the concrete strength is high, the effect of the was reduced. 4) If strength enhancement factors increased, strain at maximum load also does and ductility ratios does almost linearliary. 5) The rate of strength enhancement factor does well with the modified kent & Park's theory. 6) As to the result of calculating the effective confined area which is suggested in Sheikh & Uzumeri's theory, the middle vertical steel is effective, only if the stirrups are arranged with spacing of 3cm.

一般人의 볼링에 관한 實態調査硏究

申定勳,노형철,박인환 조선대학교 스포츠과학연구소 1995 스포츠科學硏究 Vol.7 No.-

科學 Curriculum 改善의 動向

朱忠魯 연세대학교 교육대학원 1979 연세교육과학 Vol.14 No.-

Curriculum reform movement of Science education was initiated in 1976 and the Curriculum study has been made towards formulating "Concept and process centered Science Curriculum", what is called "investigative curriculum. " Recently developed integrated and/or individualized Science curricula are also based on the idea of the above concept and process centered curriculum but extended the area that the fundamental concept fevers and/or considered not only concepts and process of the Science but also the feeling side of the students. It is quite true that the purpose of science educntion cannot be accomplished successfully even with a perfect curriculum when the ability and feeling of the students are ignored. Therefore the individualized science curriculum will be ideal one toward which the currumt study is approching. For the successful achievement of the above developed curriculum, the role of teachers are of great significance. As pointed out through the experimental teaching of PSSC course at school, it is necessary to retrain school teachers to meet the new science curriculum. Particularly in a developing country like ours, we are suffering from the lack of qualified teachers. Of course, there are a number of well trained teachers, but most school teachers in this country have received old fashioned education at home and they have not enough experience of scientific experiments to meet the new curriculum requirements. It is urgent, therefore, to establish a sound and practical retraining courses for school science teachers to carry out the new science curriculum successfully. I suggest that it is possible to retrain the school teachers at academic institution such as graduate school of education. It is also realized that the number of experiments required in the current curriculum is far beyond the practically limited available teading time at middle school. This signified that a new experimental program must be organized under the full cooperation of scientist and school teachers. Finally it must be pointed out that the supply of laboratory equipments, reagents, materials and assistants should be satisfied and the teacher's over-load should be avoided. It is quite natural that the satisfactory science education cannot be expected without a sufficient educational investigation as the good products are not produced without properly organized instruments and machines. It is clear that improvement of science education, both teaching contents and methods, must be urgently required to meet the developing and properity of this society. There is no doubt that the purpose of science education today is not any more to teach systemtic scientific knowledge as before but to teach fundamental concepts grovering today's science and scientific methods so that they may handle their own problems in the future and enjoy their lives rationally in the highly developed scientific society. Accordingly the contents of the text of science education must be carefully reselected to meet the above requirment. From the above consideration, the government officials, industrial people and school authorities have to pay their atention to organized rational programs for science education.

高强度 콘크리트를 사용한 R.C 보 部材의 付着割裂性狀에 관한 實驗的 硏究

곽노현,정헌수 중앙대학교 기술과학연구소 1993 기술과학연구소 논문집 Vol.23 No.-

In order to quantify the effect of transverse reinforcement on the bond splitting behavior of reinforcement monotonic loading tests of 6 slmply beams were carried out. The reinforcing details and material properties were so determined that the bond splitting failure proceded the shear and flexural gailure. A bond splitting strength derived from the experimental data and it accounts for folloeing paramenters : 1) Concrete Strength 2) Transverse reinforcement tatio and shape 3) Thickness of concrete cover