Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

住宅의 熱管理論

鄭震景 忠南大學校 1962 論文集 Vol.2 No.-

우리 人類는 自然環境에서 卽 雨雪寒暑에서 우리自身을 保護하기 爲하여 住居生活을 營爲하고 있는 것이다. 따라서 特히 寒暑에 對한 效果的 對策이라 하는 것은 住居生活에서 가장 重要한 것이라 하겠다. 故로 우리는 住宅의 暖房, 室內의 保溫, 防暑. 日射와 住宅과의 關係 等을 充分히 硏究하여 效果的인 方法으로서 우리環境을 좋게하며 또 燃料를 節約 함으로서 우리의 經濟生活을 나아가서는 國家經濟에 이바지하여야 할 것이다.

A Reconfigurable CMOS Power Amplifier Using Adaptive Biasing Technique for Wireless Monitoring Applications

Chung-Ching Lin,Nahid Mirzae,Ahmed Alzahmi,Howard Cam Luong,Gyung-Su Byun 대한전자공학회 2019 Journal of semiconductor technology and science Vol.19 No.6

A fully integrated CMOS power amplifier (PA) using the adaptive biasing technique for wireless monitoring is presented. The adaptive biasing scheme is employed to compensate the input capacitance of both driver stage and power stage. Utilizing the adaptive biasing technique along with the low loss 2:1 voltage-controlled switch can enable dual-modes operation and eliminate the nonlinear effect at the common source of the conventional PAs and inherently enable the PAs to become immune to the capacitive variation of transistors. In the driver bypass (DB) and high gain (HG) modes, the PA has achieved an output power (Pout) of 0 dBm, drain efficiency (DE) of 35.1% and 26% and the power gain of 1 dB, 9.6 dB, respectively, under 0.7 voltage supply. The PA is implemented in a 0.13 μm CMOS process with core area of 0.65 mm2.

An Empirical Study on the Correlation among Innovative Strategies, Motivation, Level of Implementing Innovative Activities and Business Performance in Taiwan's High-tech Manufacturers

Chung, Yi-Chan,Hsu, Yao-Wen,Chiu, Chung-Ching,Chen, Ching-Piao,Tsai, Chih-Hung The Korean Society for Quality Management 2008 The Asian Journal on Quality Vol.9 No.3

This study explores the influence of Taiwan's high-tech manufacturers' innovative strategy and innovation motivation concerning the implementation of innovative activities, as well as the influence of innovative activities implementation on business performance. The two intermediate variables, industry group and enterprise scale are also considered. Through a review of the relevant literature, a theoretical model of the influence relationship is developed, while an empirical analysis is simultaneously conducted on Taiwan's high-tech manufacturers. The research result shows that the internal driving force of innovative activities has a significant impact on the level of implementing technological innovative activities and cultural innovative activities. The external driving force of innovative activities has a significant impact on the level of implementing market innovative activities and management innovative activities. Companies adopting self-developed technology and purchased as well as self-developed technology strategies, perform better than those adopting purchased new technology or those with neither purchased nor self-developed technology strategies, at implementing technological innovative activities and cultural innovative activities. The level of implementing innovative activities has a significant influence on business performance (cost reduction and product/service differentiation). For the intermediate variables of "industry group" and "enterprise scale", it is proven in this study that they have no significant influence on the level of innovative activity implementation or business performance.

Research on the Relationship Between the OHSAS 18000 System Implementation and Competitiveness in Taiwan's Industries

Chung, Yi-Chan,Chiu, Chung-Ching,Tsai, Chih-Hung,Hsu, Yau-Wen The Korean Society for Quality Management 2006 The Asian Journal on Quality Vol.7 No.3

In recent years, the labor consciousness is gradually valued. During the process of pursuing sustainable operation, the enterprises treat occupational hazard risk as the waste of cost. The purpose of OHSAS 18000 occupational security and health management system is to systematically establish the business managerial measures in terms of occupational security and calamity prevention. This research proceeded with questionnaire analysis with respect to the relationship between the OHSAS 18000 system implementation and competitiveness in Taiwan's industries. The research result revealed that there was significant relationship between Taiwan firms' execution of OHSAS 18000 system and industry competitiveness. Thus, the enterprises can decide the proper managerial plans according to the result of their own risk evaluation to prevent the happening of accidents and reduce the operational cost. They can thus completely control business occupational hazard risk through "systematic" management.

Using Balanced Scorecard to Explore Learning Performance of Enterprise Organization

( Chung Ching Chiu ),( Chih Hung Tsai ),( Yi Chan Chung ) 한국품질경영학회 2007 The Asian Journal on Quality Vol.8 No.1

In the early industrial age which with high intensity of machine and labor, using financial measurement index was good enough to tie in company`s mechanization and philosophy of management and been in efficiency. But being comply with "New Economic age," a new economic environment is full of knowledge and information, the enterprise competition had changed from tangible assets, plants to intangible innovation ability of knowledge. As recognizing the new tendency by enterprise, they value gradually the growth and influence from learning. Practice of organization learning not only needs firm structure and be in coordination with both hardware and software, but also needs an affect measurement model to offer enterprise to estimate learning performance. It`s a good instrument of financial performance measure mold in the past years, But it`s for measuring the past, couldn`t formulate enterprise trend to future, hard to estimate investment for future, such as development of products, organization learning, knowledge management etc, as which intangible assets and knowledge ability just the key factors of being win around competition environment in the future. In 1992, Kaplan and Norton brought up Balance Scorecard (BSC) on Harvard Business Review, as an instrument helping enterprise to measure performance, which is being considered to be a most influence management instrument. It added non-financial index such as customer, internal process and learning growth besides traditional financial index, as offering enterprise an index to measure and manage intangible assets and intellectual property. As being aware of organization learning is hard to be ignored in the new economic age, this research is based on learning and growth of BSC, and citing one national material company try to let the most difficult measurement performance of organization learning, to be estimate through BSC, analyze of factor and individual case, to discuss the company how to make the related strategy and vision of organization learning to develop learning and growth of the structure of BSC, subject the matter of out put factors to be discussed, and measure the outcomes as a result of research. The research affect offers (1) the base implement procedure of carrying out BSC; (2) the reference of formulating measurement index while enterprise using BSC to estimate performance of organization learning; (3) the possibility bottleneck maybe forcing while carrying out BSC, to be an improvement or preventive for enterprise.

Iceberg in Small Pulmonary Embolism

Ching-Wei Lee,Fa-Po Chung,Kang-Ling Wang,Ching-Lan Wu,Tse-Min Lu 대한심장학회 2013 Korean Circulation Journal Vol.43 No.3

Functional and Anatomic Outcome of Uterovaginal Prolapse Repair with Transvaginal Mesh

( Ching Chung Liang ) 대한산부인과학회 2011 서울심포지움 Vol.16 No.-

Staged or concomitant surgery for correcting pelvic organ prolapse and stress urinary incontinence

( Ching Chung Liang ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

A Modified Mutation Detection Method for Large-scale Cloning of the Possible Single Nucleotide Polymorphism Sequences

( Ming Chung Jiang ),( Pao Chu Jiang ),( Ching Fong Liao ),( Ching Chiu Lee ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.2

Although the human genome has been nearly completely sequenced, the functions and the roles of the vast majority of the genes, and the influences of single nucleotide polymorphisms (SNPs) in these genes are not entirely known. A modified mutation detection method was developed for large-scale cloning of the possible SNPs between tumor and normal cells for facilitating the identification of genetic factors that associated with cancer formation and progression. The method involves hybridization of restriction enzyme-cut chromosomal DNA, cleavage and modification of the sites of differences by enzymes, and differential cloning of sequence variations with a designed vector. Experimental validations of the presence and location of sequence variations in the isolated clones by PCR and DNA sequencing support the capability of this method in identifying sequence differences between tumor cells and normal cells.