The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

인삼(Panax ginseng C.A. Meyer) 신품종 고풍의 육성경과 및 생육특성

권우생(Woo-Saeng Kwon),이장호(Jang-Ho Lee),박찬수(Chan-Soo Park),양덕춘(Deok-Chun Yang) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.2

To develop a new ginseng variety with good quality and high yielding, a lot of individual ginseng plant were selected in the farmers' fields in 1968. Among them, a promising line, 680-83-4, has been developed through comparative cultivation of several lines selected with pure line separation from local races in KT&G Central Research Institute, preliminary and advanced yield trials were performed for 8 years. 680-83-4 was designated as KG103, which was then registered as a new variety “Gopoong” with the regional yield and adaptation trials for 10 years (1981-1990) on December<br/> 27, 2000 in Korea. Gopoong has different phenotype of a dark violet stem and dark red fruit and inverted triangle shape of berries cluster as compared with other varieties. Taproot of Gopoong was longer than local race Jakyungjong, and root yield was 4.5% higher than local race Jakyungjong. In red ginseng quality, the rates of Chun-Jeesam grade(Chun and Jee means 1st and 2nd grade, respectively) were 16.6% and 9.4% for Gopoong and Jakyungjong, respectively. In these results, it was that Gopoong was superior ginseng line with good quality for manufacture of red ginseng.

외과의사의 기술적 요인과 선천성 식도폐쇄증 수술후 문합부 협착

전용순,김우기 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Background : Esophageal stricture is a common complication of anastomosis of the esophagus in esophageal atresia(EA). The factors that have been implicated in the pathogenesis in the esophageal stricture include the poor anastomotic technique(excessive tension, two-layered anastomosis, silk suture material), ischemia at the end of the esophagus, anastomotic leak, and gastroesophageal reflux. Method : To evaluate the importance of the surgeon's technique affecting anastomotic stricture after primary repair in EA, we reviewed 69 patients with EA operated at Department of Pediatric Surgery, Seoul National University Hospital from April 1978 to June 1995 and analyzed statistically the effects of such variables as sex, age at admission, age at corrective surgery, birth weight, combined anomaly, duration from admission to operation, anastomotic leakage, postoperative complication except leakage and gastroesophageal reflux, on stricture. Results : Anastomotic stricture was developed in 36(52.2%) of 69 patients who underwent primary repair of EA. There was no statistically significant factor affecting anastomotic stricture. Conclusion : The importance of cautious surgical technique should be emphasized to prevent anastomotic stricture.

분편 인플루엔자백신(split influenza vaccine)의 임상효과 및 면역원성에 관한 연구

우흥정,김동림,정희진,천병철,이주연,안정배,김지희,박찬,신영규,김우주,김민자,박승철 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 아단위 인플루엔자 백신 접종 후 백신의 인플루엔자 예방효과, 인플루엔자 방어 항체형성, 인플루엔자 백신의 안전성을 조사하고자 하였다. 방법 : 총 571명을 대상으로 인플루엔자 백신 접종을 하였고, 이들 접종자에서 인플루엔자 양질환의 이환을 조사하여 인플루엔자 백신의 인플루엔자예방효과를 알아보았고, 백신의 접종 전 및 접종 4주 후 혈청에서 혈구응집억제물(Hemagglutination Inhibition : HAI) 항체 검사를 실시하여 백신의 방어항체생성을 조사하였고, 백신의 안전성을 알아보기 위해 백신접종 후 1주일 이내의 부작용을 조사하였다. 결과 :백신 접종군과 백신 비접종군에서 인플루엔자 양 질환의 이환을 조사한 결과 접종군 28.35%, 비접종군 35.88%으로 나왔으며, p 값이 0.001로 통계적으로 접종군에서 유의하게 낮았고, 인플루엔자 양 질환의 예방 효과는 20.97%를 보였다. 백신의 방어항체 형성의 평가를 위해 유럽의 인플루엔자 백신 허가 기준을 조사하였는데 B/Guangdong/5/94균주의 백신접종 후 항체가 40이상의 비율을 제외한 다른 기준은 모두 만족 시켰다. 부작용은 전체 조사자 521명중 149명(29%)으로 주로 접종 부위의 국소 부작용을 호소했고, 전신 부작용은 2% 내외였으며 특별히 심각한 부작용은 발견되지 않았다. 결론 : 분편 인플루엔자 백신은 인플루엔자양질환의 예방과 방어항체생성에 효과 있으며 안전한 것으로 사료된다. Background : The safety and effectiveness of influenza vaccine are well known in developed country. The influenza vaccination has been recommended as one of the tentative immunization schedule for indicated persons since 1997 in Korea. But there are still no available data about them, even though nearly 5 million doses of influenza vaccine were used in 1997-1998 season. So it is immediately needed to investigate the safety. efficacy and immunogenicity of influenza vaccine among Korean. Methods : We studied the clinical efficacy of influenza vaccine by monitoring Occurrence of influenza-like illness in influenza risk group(vaccination ; 300, non-vaccination; 215) from December in 1997 to March in 1998. We used the split quadrivalent influenza vaccine containing 15 microgram of hemagglutinin of A/Beijing/262/95(HlNl), A/Wuhan/359/95(H3N2), B/Mie/1/93 and B/Guangdong/5/94. Hemagglutination inhibition(HA1) antibody titers were determined before immunization and 1 months after vaccination And we evaluated adverse effect of influenza vaccination at 7 days after vaccination. Results : Influenza vaccination was associated with si@icant reductions in influenza-like spptoms(vaccination group; 28.35%, non-vaccination group, 35.88%, p=0.001). The preventive effect of influenza-like i3lne.s among influenza risk goup was 20.97%. And immunogenicity of influenza A and B exceeded all of the European licensure criteria for immunogenicity except postvaccination proportion of titers 240 of B/Guangdong/5/94 strain. And the adverse effects were mainly local injection site problem and no serious adverse effect was noted. Conclusion : Split influenza vaccine is safe, inmunogenic and eff'tive in influenza risk group in Korea.

조직과 문화의 관점에서 접근한 한국형 지식경영 모델 : IMF 사태에 대한 사례 분석을 중심으로 A case study of the IMF stand-by arrangement in Korea

전기정,현우식,장승권 한국생산성학회 1999 生産性論集 Vol.13 No.3

Using the knowledge management perspective, this study investigates the top level decision making process, which brought the financial crisis in Korea at late 1997. To analyze the characteristics of a Korean knowledge management, this study applies knowledge spaces for meta-knowledge, formalhformal organizations, and cultural spaces. Implicit knowledge and the role of informal organizations are regarded to be relatively more important in Korean knowledge management models. It, then, suggests the introduction of meta-knowledge mechanisms for the fluent circulation of knowledge.

수종의 항진균제 치료에도 호전을 보이지 않는 만성 전신성 체부백선 1예

전세정,심우철,이은소,강원형 대한의진균학회 1998 대한의진균학회지 Vol.3 No.1

Chronic dermatophyte infection rarely fails to respond to topical or systemic antifungal therapy. Such refractory condition relates to many factors and one of them is the decreased response of delayed type hypersensitivity. A plausible mechanism by which the delayed hypersensitivity response may cause dermatophyte inhibition has been proposed already. Our patient had skin rashes for 6 years. It was diagnosed as tinea corporis and treated with various systemic antifungal agents, such as griseofulvin, itraconazole, fluconazole, terbinafine and topical forms of econazole and terbinafine. But the skin lesions did not resolve completely and reaggravated frequently. Numerous verrucae planae were found on face, neck and both extremities. Trichophyton rubrum was identified by fungus culture study. Laboratory examination showed no response against multi-CMI test, DPCP sensitization and prick test for trichophytons. We challenged the therapy with the combined antifungal agents and immune stimulatory drugs. This case is thought to be a chronic dermatophyte infection due to the defects in the both cell mediated immunity and immediate type hypersensitivity which is crucial for the host defence mechanisms against fungal infection. [Kor J Med Mycol 3(1): 43∼48]

폴리카보네이트와 폴리메틸메타크릴레이트 혼합물의 상 분리 거동

천우종,안영철 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

본 연구에서는 메틸렌클로라이드(MC)와 테트라하이드로푸란(THF)을 용매로, 헵탄을 비용매로 사용하여 용액 캐스팅법과 용액 침전법으로 비스페톨-A계 폴리카보네이트(PC)와 폴리메틸메타크릴레이트(PMMA)의 혼합물을 만들어 그의 상거동을 미분주사열량계(DSC), 광학현미경, 주사전자현미경(SEM), 융점측정장치를 사용하여 관찰하고 이들의 혼합특성을 규명하였다. 높은 분자량의 폴리카보네이트와 폴리메틸메타크릴레이트는 테트라하이드로푸란을 용매로, 헵탄을 비용매로 한 용액 침전법을 사용하면 저온에서는 단상의 혼합물을 형성하지만 고온에서는 상분리가 일어난다. 흐림점의 측정에서 나타나는 상거동은 저분자 물질들로 구성된 혼합물에서는 좀처럼 관찰되지 않는 하한임계용액온도(LCST) 현상을 보이고 폴리카보네이트 함량이 아주 많거나 적을 경우를 제외하고는 180~190℃사이의 온도에서 상분리가 일어난다. Blends of bisphenol-A polycarbonates and poly(methy1 methacrylate) have been made by both the solution casting method and the solution precipitation method using methylene chloride and tetrahydrofuran as solvents and heptane as a non-solvent, and their phase behavior and miscibility have been observed and identified by differential scanning calorimetry, optical microscope, scanning electron microscope, and melting point apparatus. The high molecular weight polycarbonate and the poly(methyl methacrylate) possibly form a blend of single phase at low temperature if prepared by the solution precipitation method using tetrahydrofuran as a solvent and heptane as a non-solvent, though phase separation occurs at high temperature. The cloud point of the blend shows a lower critical solution temperature(LCST) behavior which is hardly observed in a blend composed of low molecular weight materials, and the ranges of its LCST have been found to be 180-190 C except for the blends having particularly high or low content of polycarbonate

山野地에 있어서 標高別 施肥水準의 改良草地의 生産性 및 植生變化에 미치는 影響

全宇福,金元鎬 全南大學校 農漁村開發硏究所 1987 農業科學技術硏究 Vol.22 No.-

This experiment was carried out to evaluate the effect of three rates of N-P2O5-K2O (Light; 140-100-100. Standard: 280-200-240, Increase: 420-400-4007kg/ha), altitude(350m and 500m) and slope exposure(north and south) on dry matter yield, quality and botanical composition of orchardgrasa dominated mountain pasture. The results obtained were summarized as follows: 1. As fertilizer application increased. the dry matter yield of grasses was increased, and average dry matter yield on the north slope was more increased than that on the south slope and 500m than 350m, respectively. 2. The increase of NPK rate resulted in increment of crude protein content of grasses, and crude protein content on the north slope was higher than that on the south slope and 500m than 350m, respectively. However, NDF and ADF content and in-vitro dry matter digestibility was inconsistently affected by their application rates. 3. By increasing the NPK rate, the yield of crude protein of grasses was increased. and the yiels of crude protein on the north slope was more increased than that on the south slope and 500m than 350m, regpectively. 4. The yield of NDF, ADF and in-vitro digestible dry matter was increased as fertlllzer application increased but their differences between standard and increase plot were not significant. The yield of those on the north slope was more increased than that on the south slope and 500m than 350m, respectively. 5. By increasing the NPK rate, the percentage of orchardgrdgrass in botanical composition was increased greatly at all plots, whereas that of the weed and ladino clever was increased at light fertilization plot.

다공성 폴리에틸렌 삽입물(Medpor^(�))을 이용한 새로운 세포학적 창상치유 실험모델 : Porous Polyethylene Implantation

전경욱,한승규,김우경 大韓成形外科學會 2004 Archives of Plastic Surgery Vol.31 No.2

1910년대 객주 통제와 '朝鮮會社令'

전우용 역사문제연구소 1997 역사문제연구 Vol.- No.2