The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

슬라브主義에 나타난 民主主義理念의 特性

千亨均 군산대학교 1975 群山水産專門大學 硏究報告 Vol.9 No.3

Thiocholine ester 기질을 이용한 Acetylcholinesterase 활성부위의 구조특성

이천배,주은희,최수라,석대은,명평근 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

The inhibition pattern of three inhibitors(tacrine, decamethonium and propidium) on the hydrolysis of various thiocholine ester substrates by eel acetylcholinesterase was comparatively examined. When the substrate was acetylthiocholine, it showed a similar competitive inhibition by tacrine inhibitor, and a mixed type inhibition by decamethonium and propidium inhibitors. When the substrate was pentanoylthiocholine, it showed an uncompetitive inhibition by tacrine, and a noncompetitive inhibition by decamethonium. When the substrate was laurylthiocholine, it showed mixed type and uncompetitive inhibition by tacrine, and a competitive inhibition by decamethonium and propidium. Those results suggest that the active of acetylcholinesterase has the existence of hydorphobic site besides the anionic and esteratic site.

원격 다중 PLC 제어용 다중 통신 모듈 개발

현웅근,유춘식 호남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.8 No.-

A wireless communication module for multiple PLC was developed. The function of the developed system are dual communication , concurrent multiple communication, PLC control ,d interpretation of PLC command, and A/D D/A converting. The multiple communication approach Is based on master-slave control concept. To show validity of the developed module, severial experiments are illustrated.

임대아파트의 수선비용 관리시스템을 위한 데이터베이스 구축

이춘경,정영한,박태근,김광우,김창덕,김옥규,황영삼,박홍석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

It shows that a variety of side effects result from the housing-supply policy focused in quantity. In a rental apartment for the low-income group, a shortage of maintenance cost in facilities become aggravated. The rapid progress of obsolete leads the lessor to the economic loss as well as a baneful influence to national economy. Also, the maintenance skill brings inadequacy by reason of technique development focused on new construction. As a result, the cost which needs for maintenance should get accomplished to application toward economy analysis and attempt considered management and financial affairs. Simultaneously, it should keep and administer a lot of data of maintenance affairs during life cycle in apartment and set up the way for aiding the situation of maintenance process.

고등학생의 여가활동 참가가 학교생활적응에 미치는 영향

신근춘,문병용 江原大學校附設體育科學硏究所 2004 江原大學校附設體育科學硏究所論文集 Vol.- No.27

The purpose of this study would likt to find out reached influence of school life adaptation for senior high school students'leisure activity participation. And I established to attain aim of research such like research subject. First, difference of type for leisure activity about area-sexual distinction·monthly income·pocket money, second, difference of school life adaptation about area·sexual sistinction·monthly income·pocket money, third, reached influence of school life adaptation for leisure activity participation extent, fourth, I established to attain the subject for research that would like to find out relation leisure activity type and school life adaptation. I set up seniou high school make and female students of Kangwon Do Yeongseo area with a population for this research Moreover, the selected group was drawn samples each 2 schools second grade 150 students of C·H area malefemale with stratified cluster random sampling . I used questionnaire totally 496 students for material analysis. I used questionnaire that was made recomposition with proper research. I also used measuring implement that used it - P.E. activity participation of Juvenile and questionnaire for adaptation of school life> (Park Jeong Hyeon 1999) <Survey of type for leisure activity> (Ministry of Culture P.E. 1995). Reliance data of questionnaire for school life adaptation used 'Cronbach'α, the coefficient for reliance data was mentioned 6812_6935. Statistics methods - which were used this research are χ^(2)-test, t-test, one-ANOVA, correlation analysis, multiple regression analysis, two-way ANOVA etc. First, when I analyzed difference of type for leisure activity by area·sexual distinction·monthly income·pocket money, I found that it doesn't have any gap by it besides sexual distinction made difference between sports activity for male students and viewing appreciation for female students. Second, differences of adaptation for school life by area·sexual distinction·monthly income·pocket money made several situations. For instance, areamonthly income made difference teacher's relation·friends relation·classes in school, sexual sistinction made it friends relation·school class, and pocket money made it school rules. Third, reached influence of school life adaptation for leisure activity participating extent showed that participating period affected friends relation, participating frequency did teacher's relation, and participating strength did school classes. Fourth, difference leisure activity type and school life adaptation showed that student who did activity for hobbygeneral education made significant difference.

殺蟲性 O,O-Diethylphenylphosphate 誘導體들에 의한 Acetylcholinesterase의 Phosphorylation에 關한 量子藥理學的 硏究

明平根,成洛道,柳柄泰,李天培 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-

Quantum pharmacological study has been carried out for the phosphorylation process of acetylcholinesterase (Ach E) and hydrolysis reaction of O,O-diethylphenylphosphate (DPP) derivatives. Quantum variables of DPp molecule were calculated by extended Hu¨ckel theory (EHT) molecular orbital calculation method and linear free energy relationship(LFER) parameters were applied to the Hamett, Taft & Swain-Luption's simple and dual substituents parameter equation. The results shows that the field effect (F) was larger than resonance effect (R) and the values of percent field (%F) and percent resonance (%R) were about %F=34 and %R=66, respectively, yielding the ratio of F to R of 2:1. From the regression analysis, the various quantum chemical parameters, the net charge of phosphorus atom and that of meta carbon atom, the LUMO energy and the electrophilic superdelocalizability were influenced significantly on the phosphorylation of Ach E. The charge-controlled and orbital-controlled reactions proceed via trigonal bypyramidal penta-coordinated intermediate with pseudorotation.

DNA Conformation Probe로서의 Anionic Potential Reporter 분자의 구조 연구 : Ⅰ. Nalidixic Acid의 공간군 결정 Ⅰ. The Space Group Determination of Nalidixic Acid

오인근,고동성,이천배,서정인,서일환 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.1

The space group of nalidixic acid(1-ethyl-1, 4-dihydro-7-methyl-4-oxo-1, 8-naphthyridine-3-carboxylic acid, C_12H_12N_2O_3), a clinically important antibiotics, has been determined by single crystal X-ray photographic data collected three-dimensionally using Weissenberg method with Cu Kα radiation (λ=1.5418 Å), as the structural data is required and important for the elucidation of the molecular mechanism of the inhibitory action of the antibiotics on DNA synthesis. The space group is P2_1/c with four molecules in the unit cell of dimensions, a=8.98±0.15, b=13.30±0.03, c=9.50±0.16Å, and β=99.5±0.1°.

어린 가토에서 자가 연골 이식후 연골성장에 관한 실험적 연구

권택근,김태연,정전은,이동진 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

Does free transplanted autologous cartilage grow? Until now many autors have published different results about the growth potential of cartilage. Some authors have produced in their study that transplanted cartilages were able to grow, but others showed contradicting results. In this study, authors obtained cartilages from young rabbits'ear, 10 by 10mm in size and divided them into two groups ; the first group was cartilages with perichondrium and the second group was cartilages stripped perichondrium. We transplanted both groups of cartilages in the posterior nuchal area of rabbits. Then numbers of specimens of each group were evaluated and compared for assessment of cartilage growth. After observaton for six months, we took the cartilages from the posterior nuchal area and examined the surface area, the thickness, and the weight and finally confirmed the growth potential of the cartilage by histologic study. The results are as follows : 1, The surface area, the thickness, and the width of the transplanted cartilages were increased significantly in gross specimens and their measured values were statistically significant and cartilages with perichondrium showed more growth than cartilages stripped perichondrium 2. In histologic studies, we observed increased surface activities with numerous proliferatng cells and found no abnormal features compared with normal cartilages. Cartilages with pericondrium were thicker than cartilages with stripped perichondrium

Thiocholine ester 기질을 이용한 Acetylcholinesterase 활성부위의 구조특성

이천배,주은희,최수라,석대은,명평근 충남대학교 기초과학연구소 1999 忠南科學硏究誌 Vol.26 No.1

The inhibition pattern of three inhibitors(tacrine, decamethonium and propidium) on the hydrolysis of various thiocholine ester substrates by eel acetylcholinesterase was comparatively examined. When the substrate was acetylthiocholine, it showed a similar competitive inhibition by tacrine inhibitor, and a mixed type inhibition by decamethonium and propidium inhibitors. When the substrate was pentanoylthiocholine, it showed an uncompetitive inhibition by tacrine, and a noncompetitive inhibition by decamethonium. When the substrate was laurylthiocholine, it showed mixed type and uncompetitive inhibition by tacrine, and a competitive inhibition by decamethonium and propidium. Those results suggest that the active site of acetylcholinesterase has the existence of hydrophobic site besides the anionic and esteratic site.