The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

생태계의 생명부양 능력과 환경신학

전헌호 가톨릭대학교 사목연구소 2000 司牧硏究 Vol.8 No.-

환경문제에 관한 교회의 발표가 환경문제 극복에 미친 영향에 관한 小考

全憲浩 대구효성가톨릭대학교 1996 연구논문집 Vol.52 No.1

광주·전남 지역에서 발생한 스포로트리쿰증 103예에 대한 임상적 고찰(1967-1996)

전인기,김훈희,김성진,이승철,원영호 대한의진균학회 1997 대한의진균학회지 Vol.2 No.2

Background : Sporotrichosis is most common deep cutaneous fungal disease in Korea. It is markedly variable in incidence, distribution, and colinical pattern. The epidemiology have been well known to change over years, and to be influenced by environmental condition and geographic location. Sporotrichosis in Kwangju and Chonnam area is more prevalent compared to other areas in Korea. Objective : Our purpose was to describe the prevalence, epidemiological and clinical features, regional distribution and the change of the incidence according to the climate in different regions. Method : the observation of 103 sporotrichosis patients confirmed by clinical, histopathological and mycological studies had been made in the Department of Dermatology at Chonnam National university Hospital for thirty years from 1967 to 1996. Results and Conclusion : The sporotrichosis patient was 0.09% of 108, 680 outpatients, prevalent at the age of thirties and forties(20%), and 3.2:1 in the ration between male and female. The 58% of total cases visited us within 3 months after the beginning of skin lesion. Almost all initial lesions appeared on the exposed areas, especially upper extremities(68%). The 74% of the total cases was lymphocutaneous type, and the rest was fixed cutaneous type. Most popular occupation of patients was farmer(60%). The 69% of patients visited in spring and winter, especially in february(17%). The incidence of sporotrichosis per average 100,000 population in districts of Kwangju and Chonnam area revealed a difference with the amount of rainfall in winter; average 1.8 person in an area above 125㎜,0.8 person in an area between 100㎜ and 125㎜, and 0.2 person in an area below 100㎜ These rsults suggest that the incidence and clinical features of sporotrichosis are influenced by occupations, leisure and occupational activities, living conditions, and the climate.

발달장애유아의 언어행동발달 연구

김춘희,변찬석,서경희,유은정,이효신,전헌선,최성규 大邱大學校 特殊敎育·再活科學硏究所 2000 特殊敎育硏究 Vol.23 No.1

본 논문은 발달장애유아 언어행동발달 컨텐츠 개발에 요구되는 기초연구로써 연구의 이론적 바탕을 확립하고자 하였다. 연구에 사용된 방법은 문헌조사 방법 및 현장 인터뷰기법을 병행하였다. 본 연구의 주된 목적은 크게 3가지로 구분된다. 첫째, 발달장애유아의 가정 내 교육 욕구를 분석하고자 하였다. 둘째, 발달장애유아의 언어발달 양상을 검토하고자 하였다. 세 번째 목적은 유아의 언어행동발달 컨텐츠 구성의 주요 지침을 설정하고자 하였다. 이상의 연구목적에 따른 연구결과를 요약하면 다음 몇 가지로 제시된다. 1.가정 내 부모-유아간 관계형성이 우선 고려 되어야하고, 이에 따른 상호작용은 발달적 애착형성이 가능한 방향으로 정립되어야한다. 2.발달장애유아의 언어행동발달양상을 검토한 결과에 따르면, Piaget는 언어행동발달에 있어 감각운동기적 환경탐색 기능을 강조하고 있고, 이는 현재까지 중요한 유아 발달의 기저로 정착되었다. 따라서, 발달장애유아의 언어행동발달을 위한 컨텐츠 개발 내용에 있어서도 이러한 기저에 충실함이 합리적이다. 3.발달장애유아들에게는 언어행동 양상의 갈래가 크게 운동장애 및 감각결함으로 나누어질 수 있으므로, 이 두 가지 갈래의 각기 다른 특성이 컨텐츠 구성에서 충분히 고려되어야한다. 4.자폐성과 같은 중증 전반적 발달장애 유아를 위한 언어행동발달 컨텐츠 구성에서 특히 고려해야 할 두 가지 요소는 모방행동기능과 반복단순훈련기능 향상으로 결론지을 수 있다. This article planed to establish the theoretical foundations of the research as a basic study needed for developing the contents of verbal(language) behavior development for the infants with developmental disabilities. The methods used in the study was previous literature researches and interviews with subjects. The purposes of the study were divided into three parts. First, it was to analyze the educational needs of the infants with developmental disabilities in home settings. Second, it was to review and discover the language patterns of the infants with developmental disabilities. The third purpose was to devise the major guidelines of their language development's contents. The results of the study were described as the followings. 1.The relationships between the infants with developmental disabilities and their parents and their interactions should move to build the developmental attachments between them. 2.According to the literature findings of their language development patterns, Piaget concentrated on the functions of sensory motor environmental exploring in the language behavior development. The functions have been settled down into the important foundation for the infants' development. Thus, it was resonable that the content's development of the language behaviors for the infants with developmental disabilities should based on the function addressed by Piaget. 3.The language behaviors' patterns of the infants with developmental disabilities generally were classified into motor disabilities and sensory defect(deficiency). Therefore, the two factors should be considered for the constructions of the contents. 4.Constructing the contents of the language behavior developments for the infants with severely pervasive developmental disabilities such as autism, the improvements of two functions, imitative behavior and repetitive simple training should be considered.

공구 수명의 신뢰성 예측 프로그램 개발

이수훈,김봉석,강태한,송준엽,강재훈,서천석 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

This paper deals with a prediction method of tool life in view of the reliability assessment. In this study, the flank wear was studied among multi-factors deciding the tool wear state. Firstly, tool life was predicted by correlation between flank wear and cutting time, based on the extended Taylor tool life equation of turning data, including parameters of cutting speed, feed rate, and cutting depth. Secondly, each of cutting conditions of endmilling was equivalently converted to apply ball endmill data to the extended Taylor equation. The web-based reliability prediction program for tool life is being developed as one of reliability assessment programs to for the machine tools.

반도체 공정에서의 기상 세정장비 개발에 관한 연구

박헌휘,이춘수,최승우,함승주 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

저압 기상 영역에서 Anhydrous HF 가스와 Methanol vapor를 사용하는 산화막 식각공정을 수행하기 위하여 (1) 반응기 부피의 최소화, (2) 공정압력의 최소화, (3) 고순도 알루미나 Reactor 적용, (4) Cluster화의 개념을 적용한 VPC 장치를 제작하였다.Wafer의 온도, HF의 분압 및 Working Pressure 등의 공정변수에 따른 Oxide Wafer의 식각특성의 변화를 확인하였다 또한 Etch Uniformity를 향상시키기 위하여 Shower Head의 구조를 변경시켜서 실험하였으며, CFD Simulation을 이용하여 Reactor 내에서의 HF gas 및 Methanol vapor의 분율을 예측하였다.

전자산업 협력업체 안전관리 개선방안 연구

김헌수,박해천 조선대학교 생산기술연구소 2005 生産技術硏究 Vol.27 No.2

Lately, electronics industries manufacture electronic products in cooperation between mother companies producing finished goods and sub-contractors delivering parts to them. So, in electronics industries, not only are the manufacturing activities of mother companies important but also are the manufacturing activities of sub-contractors supplying various components to their customers important. With this environment, it is difficult to survive in the global competitions not only for the parts suppliers but also for the customers being supplied with parts by their part sub-contractors. But, it is getting specialized so the customers could not survive without their sub-contractors. So, the customers need to consider their suppliers as their extended production lines and introduce a comprehensive safety management support system to prevent industrial accidents by implementing systematic safely management support activities.

Cyclosporine이 同種皮膚移植瓣의 生存에 미치는 影響에 關한 實驗的 硏究

金世泳,趙尙憲,丁銓殷 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

With efficient but nontoxic immunosuppression, allogenic skin could be used clinically on early treatment of severe burns or transfer of composite tissue allograft. Unlike organ grafts, where cyclosporine is superior to previously used agents (namely, azathioprine, steroid, 6-mercaptopurine etc.) in clinical setting, the behavior of allogenic skin under prolonged cyclosporine therapy has rarely been studied. The aim of this study is to prolong significantly the survival of skin allografts in rat model using the lowest possible doses of cyclosporine. Author studied the immunosuppressive effects of short courses of the drug, the efficacy of various maintenance doses in producing long-term survival, and the posibility of reversing episodes of rejection phenomenon. The results were obtained as follows: 1. Mean survival times (MST) of skin graft in untreated recipients were similar in both strain combinations tested (MST ± SD=8 ±1 days in Sprague Dawley to Gunns rat and 7 ±1 days in Gunns to Sprague Dawley rat). The short daily courses of 7,14 and 21 days of cyclosporine therapy significantly prolonged the survival in all groups. 2. Striking prolongation of skin graft survival was produced using maintenance doses of 8 or 15 ㎎/㎏ given every fourth day following 14 days of daily doses; lower maintenance doses were ineffectual. 3. Even long-standing allografts could undergo complete rejection following cessation of the drug. Within 2 to 4 weeks after discontinuation of the drug, early signs of rejection were noted. For reversing such rejection episodes, resuming cyclosporine administration in regular doses of 15㎎/Kg/day for 7 days,followed by the same dose given every second day, then every third day, and finally maintenance schedule every fourth day was done. Such early rejection were stopped within a week and the graft was healed completly by the third week.

Pseudosynovial Sheath (가골액초)와 Polytetrafluoroethylene (Goretex)를 通한 神經再生의 實驗的 硏究

楊斗炳,趙尙憲,丁銓殷 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.1

The experimental study was performed to determine whether autogenous pseudosynovial sheath and polytetrafluoroethylene (Goretex), teflon-derivatives might serve as a conduit for nerve regeneration. Also, two different experimental conduits were compared with that of normal control group by gross external findings, histologic and electrophysiologic examinations at intervals. The following results were obtained; 1. Effective nerve regeneration was seen through conduits within 2 months (5-mm nerve gap group) and 3 months (10-mm nerve gap group) after repair. 2. Results of nerve conduction study revealed average 75% (Pseudosynovial group) and 33% (Goretex group) of normal control value in case of 5-mrn nerve gap at 3 months after repair. 3. Pseudosynovial sheath group demonstrated more functional recovery and histologic regeneration than Goretex group.