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        • KCI등재SCOPUS

          Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases

          Xinxia,Zhao,Wei,Ni,Chuangfu,Chen,Wujiafu,Sai,Jun,Qiao,Jingliang,Sheng,Hui,Zhang,Guozhong,Li,Dawei,Wang,Shengwei,Hu 아세아·태평양축산학회 2016 Animal Bioscience Vol.29 No.3

          Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in tandem with singlestranded DNA oligonucleotides (ssODNs). We designed a pair of TALENs to target a highly conserved sequence in the coding region of the sheep MSTN gene. The activity of the TALENs was verified by using luciferase single-strand annealing reporter assay in HEK 293T cell line. Co-transfection of TALENs and ssODNs oligonucleotides induced precise gene editing of myostatin gene in sheep primary fibroblasts. MSTN gene-edited cells were successfully used as nuclear donors for generating cloned embryos. TALENs combined with ssDNA oligonucleotides provide a useful approach for precise gene modification in livestock animals.

        • SCIESCOPUSKCI등재

          Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases

          Zhao,,Xinxia,Ni,,Wei,Chen,,Chuangfu,Sai,,Wujiafu,Qiao,,Jun,Sheng,,Jingliang,Zhang,,Hui,Li,,Guozhong,Wang,,Dawei,Hu,,Shengwei Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3

          Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in tandem with single-stranded DNA oligonucleotides (ssODNs). We designed a pair of TALENs to target a highly conserved sequence in the coding region of the sheep MSTN gene. The activity of the TALENs was verified by using luciferase single-strand annealing reporter assay in HEK 293T cell line. Co-transfection of TALENs and ssODNs oligonucleotides induced precise gene editing of myostatin gene in sheep primary fibroblasts. MSTN gene-edited cells were successfully used as nuclear donors for generating cloned embryos. TALENs combined with ssDNA oligonucleotides provide a useful approach for precise gene modification in livestock animals.

        • SCIESCOPUSKCI등재

          A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus

          Yanqiu,Wei,Baozhi,Yang,Yunlong,Li,Yongcheng,Duan,Deyu,Tian,Baoxiang,He,Chuangfu,Chen,Wenjun,Liu,Limin,Yang 대한수의학회 2020 JOURNAL OF VETERINARY SCIENCE Vol.21 No.4

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

        • KCI등재SCOPUS

          A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

          Junbo,Zhang,Shuanghong,Yin,Fei,Guo,Ren,Meng,Chuangfu,Chen,Hui,Zhang,Zhi-qiang,Li,Qiang,Fu,Huijun,Shi,Shengwei,Hu,Wei,Ni,Tiansen,Li,Ke,Zhang 한국미생물학회 2014 The journal of microbiology Vol.52 No.8

          Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

        • KCI등재SCOPUS

          Prevalence of Hydatid Cysts in Livestock Animals in Xinjiang, China

          Meng,Qingling,Wang,Guanglei,Qiao,Jun,Zhu,Xinquan,Liu,Tianli,Song,Xuemei,Zhang,Jinsheng,Wang,Huisheng,Cai,Kuojun,Chen,Chuangfu 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.3

          Hydatid worms, hosted by humans and animals, impose serious human health risk and cause significant live-stock production loss. To better understand the disease infection status in Xinjiang, China, we investigated the disease epidemics in 4 livestock animals, i.e., cattle, sheep (both sheep and goat), camels, and horses, slaughtered at the abattoirs in Urumqi, Yining, Tacheng, and Altay areas. The results showed that the animals were infected at different rates, in the order of sheep (9.8%), cattle (8.4%), camels (6.8%), and horses (4.3%). The infection rates were found to be different between the abattoirs in various regions even for the same animals. For sheep, the rates increased significantly as the animals grew older. It was 1.9% before 1 year of age and increased to 8.2% in the age of 1-2 years, and further increased to 12.3% when the animals were 3-4 years old, and reached 17.2% when they were 5-6 year old. Sheep older than 6 years had an infection rate of 19.5%. This study demonstrates that the 4 livestock animals in the pastoral areas in Xinjiang were infected by the parasites to various extend. This study is the first systematic investigation of the hydatid worms in various livestock animals in Xinjiang, China, which provides epidemiological information about the infection of hydatid worms in livestock, and is valuable in developing strategies for prevention and control of the hydatid disease.

        • KCI등재SCOPUS

          Immunization of BALB/c mice with Brucella abortus 2308DwbkA confers protection against wild-type infection

          Zhi-qiang,Li,Dan,Gui,Zhi-hua,Sun,Jun-bo,Zhang,Wen-zhi,Zhang,Hui,Zhang,Fei,Guo,Chuangfu,Chen 대한수의학회 2015 JOURNAL OF VETERINARY SCIENCE Vol.16 No.4

          Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.

        • KCI등재SCOPUS

          Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells

          Qiang,Fu,Huijun,Shi,Yan,Ren,Fei,Guo,Wei,Ni,Jun,Qiao,Pengyan,Wang,Hui,Zhang,Chuangfu,Chen 한국미생물학회 2014 The journal of microbiology Vol.52 No.7

          Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

        • SCIESCOPUSKCI등재

          Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

          Yan,,Bin,Liu,,Xiafei,Wu,,Junyuan,Zhao,,Shanshan,Yuan,,Wumei,Wang,,Baoju,Wureli,,Hazi,Tu,,Changchun,Chen,,Chuangfu,wang,,Yuanzhi The Korean Society for Parasitology 2018 The Korean Journal of Parasitology Vol.56 No.4

          Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.

        • KCI등재SCOPUS

          Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

          Bin,Yan,Xiafei,Liu,Junyuan,Wu,Shanshan,Zhao,Wumei,Yuan,Baoju,Wang,Hazi,Wureli,Changchun,Tu,Chuangfu,Chen,Yuanzhi,wang 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.4

          Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.

        • SCIESCOPUSKCI등재

          Prevalence of Hydatid Cysts in Livestock Animals in Xinjiang, China

          Qingling,,Meng,Guanglei,,Wang,Jun,,Qiao,Xinquan,,Zhu,Tianli,,Liu,Xuemei,,Song,Jinsheng,,Zhang,Huisheng,,Wang,Kuojun,,Cai,Chuangfu,,Chen The Korean Society for Parasitology 2014 The Korean Journal of Parasitology Vol.52 No.3

          Hydatid worms, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. To better understand the disease infection status in Xinjiang, China, we investigated the disease epidemics in 4 livestock animals, i.e., cattle, sheep (both sheep and goat), camels, and horses, slaughtered at the abattoirs in Urumqi, Yining, Tacheng, and Altay areas. The results showed that the animals were infected at different rates, in the order of sheep (9.8%), cattle (8.4%), camels (6.8%), and horses (4.3%). The infection rates were found to be different between the abattoirs in various regions even for the same animals. For sheep, the rates increased significantly as the animals grew older. It was 1.9% before 1 year of age and increased to 8.2% in the age of 1-2 years, and further increased to 12.3% when the animals were 3-4 years old, and reached 17.2% when they were 5-6 year old. Sheep older than 6 years had an infection rate of 19.5%. This study demonstrates that the 4 livestock animals in the pastoral areas in Xinjiang were infected by the parasites to various extend. This study is the first systematic investigation of the hydatid worms in various livestock animals in Xinjiang, China, which provides epidemiological information about the infection of hydatid worms in livestock, and is valuable in developing strategies for prevention and control of the hydatid disease.

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