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사전령,김진규,권동주,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-
강원대학교 구내림에서 오동나무 잎을 채취하여 아세톤-물(7:3, v/v)로 추출하고 n-hexane, methylene chloride, ethylacetate 및 H_(2)O 등 네 개의 분획으로 분리한 후 에틸아세테트용성 분획물을 Sephadex LH-20으로 칼럼 크로마토그래피를 수행하였으며 용리용매는 메탄올 수용액과 에탄올-핵산 혼합액을 사용하였다. 그 결과 flavonoid류 화합물인( +)-catechin, ( - )-epicatechin, apigenin, luteolin, taxifolin과 phenolic acid류 화합물인 p-hydroxybenzoic acid, vanillic acid 및 p-coumaric acid를 단리 하였다. 단리된 화합물은 NMR 및 MS 스펙트럼을 이용하여 구조를 결정하였다. The leaves of Paulownia Coreana Uyeki were collected, extracted with acetone-H_(2)O(7:3, v/v), fractionated with n-hexane, methylene chloride and ethylacetate, and freeze dried to give some dark brown powder. The ethylacetate soluble mixture was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Spectrometric analysis such as NMR and MS From the ethylacetate fraction, five flavonides and three phenolic acids were isolated and determined.
사전령,권동주,김진규,황병호,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-
일본잎갈나무 낙엽 (8.5kg)을 채취하여 9.5% EtOH 용액으로 추출하고 농축된 추출용액은 분획깔때기로 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성과 수용성 분획에 대하여 칼럼크로마트크래피를 실시하였고, 충진물질로는 Sephadex LH-20을, 용리용매로는 메탄올 수용액 및 에탈올-핵산 혼합용액을 사용하였다. 단리된 화합물들은 TLC로 확인한 후 NMR 스펙트럼을 사용하여 정확한 구조규명을 하였고 FAB-MS와 EI-MS 스펙트럼으로 분자량을 측정하였다. 일본잎갈나무 낙엽으로부터 5개의 화합물을 단리하였으며, 각 분획물과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화 시험을 실시하였다. Fallen neeldes (8.5kg) of Larix kaempferi were separately collected. extracted with 95% EtOH. EtOH extract was evaporated under reduced pressure, concentrated then successively fractionated with a series of hexane. methylene chloride, ethylaectate and water on a separatory funnel. Then, each fraction was freeze dried. A portion of ethylacetate and water soluble powder were packed on a column chromatography (Sephadex LH-20) eluting with aqueous MeOH and EtOH-hexane mixture. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds.
Chuan-Te Tsai,Yung-Ming Chang,Shu-Luan Lin,Yueh-Sheng Chen,Yu-Lan Yeh,Viswanadha Vijaya Padma,Chin-Chuan Tsai,Ray-Jade Chen,Tsung-Jung Ho,Chih-Yang Huang 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.3
Angiotensin II (Ang II) is a very important cardiovascular disease inducer and may cause cardiac pathological hypertrophy and remodeling. We evaluated a Chinese traditional medicine, alpinate oxyphyllae fructus (AOF), for therapeutic efficacy for treating Ang II-induced cardiac hypertrophy. AOF has been used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. We investigated its protective effect against Ang II-induced cytoskeletal change and hypertrophy in H9c2 cells. The results showed that treating cells with Ang II resulted in pathological hypertrophy, such as increased expression of transcription factors NFAT-3/p-NFAT-3, hypertrophic response genes (atrial natriuretic peptide [ANP] and b-type natriuretic peptide [BNP]), and Gαq down-stream effectors (PLCβ3 and calcineurin). Pretreatment with AOF (60–100 μg/mL) led to significantly reduced hypertrophy. We also found that AOF pretreatment significantly suppressed the cardiac remodeling proteins, metalloproteinase (MMP9 and MMP2), and plasminogen activator, induced by Ang II challenge. In conclusion, we provide evidence that AOF protects against Ang II-induced pathological hypertrophy by specifically inhibiting the insulin-like growth factor (IGF) II/IIR-related signaling pathway in H9c2 cells. AOF might be a candidate for cardiac hypertrophy and ventricular remodeling prevention in chronic cardiovascular diseases.
Roles of mTOR and p-mTOR in Gastrointestinal Stromal Tumors
Li, Jun-Chuan,Zhu, Hong-Yu,Chen, Ting-Xuan,Zou, Lan-Ying,Wang, Xiao-Yan,Zhao, Hui-Chuan,Xu, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Objective: This study aimed to examine the relationship between expression of mammal target of rapamycin (mTOR) and phosphorylation of mTOR (p-mTOR) protein in the PI3K/Akt/mTOR signaling pathways in gastrointestinal stromal tumors and relatiuonships with clinical factors. Methods: Immunohistochemistry was used to detect the expression of the associated proteins mTOR, p-mTOR, and phosphorylation of the tumor suppressor genes PTEN, P27, VEGF, and EGFR in 40 cases of gastrointestinal stromal tumors, with division into a very low and low risk group as well as a moderate and high risk group. Results: The positive rate of mTOR and p-mTOR was significantly increased in the moderate and high risk group compared with the very low and low risk group. The difference was statistically significant (P<0.05). When grouped according to size, the positive mTOR expression rate exhibited a statistical difference (P<0.05), which was significantly increased in the group of tumors larger than 5 cm. The difference in the positive mTOR and p-mTOR expression rate exhibit no statistical significance among the PTEN, P27, VEGF, and EGFR expression subgroups (P>0.05). Conclusion: The different expressions of mTOR and p-mTOR in the signal transduction pathway of gastrointestinal stromal tumor in the different degree-of-risk groups suggested that the mTOR and p-mTOR of the signal transduction pathway serve an important function in the occurrence and development of gastrointestinal stromal tumors.
Chuan Liu,Jia-bin Shen,Chang-hua Lin,Jian-fei Wang,Jian-xin Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5
Ultrasonic impact treatment (UIT) was carried out on three specimens with different strengths and, to measure the subsurface stress distribution of the specimens, the layer-by-layer removal x-ray diffraction method was applied. The stress redistribution due to material removal was corrected by finite element analysis. The effect of material strength on the after-UIT subsurface stress was investigated. The results show that the UIT can cause a compressive stress layer with a depth of over 2.5 mm for specimens with different strengths, while the material strength has no significant effect on the depth of UIT-induced stress. The UIT-induced peak compressive stresses in the three specimens exceed the material yield strength, appearing at the depth of 0.2 mm beneath the surface and increasing with the increase of material yield strength. The ratio of UIT-induced peak compressive stress magnitude to material yield strength decreases with the increase of material yield strength.
Chuan Zhang,Liwen Cui,Chonghuai Liu,Xiucai Fan,Jinggui Fang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3
The distal end of grape berries preferentially cracks during the soaking process in vitro, which may be related to preferentialwater uptake and cell anatomy. Thus, the relationship between water uptake and cell anatomical structure was assessed using49 grape varieties. In vitro immersion experiments were performed on mature berries to measure the diff erences in berrycrackingproperties. A whole-berry in vitro staining method was used to trace the water uptake of the vascular bundles, andparaffi n sections were used to observe and analyze the structural parameters of the diff erent tissues. The results showed thatthe berry-cracking rate and water uptake of the distal end of berries prone to cracking were signifi cantly higher than that ofthe resistant berries. Compared with the berries that were resistant to cracking, the berries that were prone to cracking possesseda thin cuticle, epidermis and sub-epidermis. Dye accumulated in the distal end of the cracking-prone berries, whicheven exhibited cracking during the dyeing process. However, less dye accumulated on the surface of the cracking-resistantvarieties and was only sporadically distributed on the surface. In addition, cracking of the distal end of the berry may alsobe related to other shape and size parameters of the cells in cell layer. Analysis of vascular bundle water transport and thediff erences in the cell structure characteristics of the diff erent varieties, off ered a preliminary explanation for why the distalend of the berry is prone to cracking, providing theoretical support for further research on the mechanisms of berry cracking.
Chuan He,Caixia Su,Wentong Zhang,Qin Zhou,Xu Shen,Junjie Yang,Naixian Shi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.3
Purpose: This study aimed to elucidate whether lncRNA ZFAS1 is involved in neuronal apoptosis and inflammation in temporal lobe epilepsy (TLE). Materials and Methods: Ninety-six TLE patients were recruited, and their peripheral venous blood was gathered to determine Zfas1 expression with polymerase chain reaction. Neurons were separated from hippocampal tissue of newborn SD rats, and siZfas1 or pcDNA3.1-Zfas1 was transfected into the neurons. Inflammatory cytokines released by neurons were determined, and neuronal activities were evaluated through MTT assay, colony formation assay, and flow cytometry. Results: Serum levels of Zfas1 were higher in TLE patients than in healthy controls (p<0.05). Furthermore, Zfas1 expression in neurons was raised by pcDNA3.1-Zfas1 and declined after silencing of Zfas1 (p<0.05). Transfection of pcDNA-Zfas1 weakened the viability and proliferation of neurons and increased neuronal apoptosis (p<0.05). Meanwhile, pcDNA3.1-Zfas1 transfection promoted lipopolysaccharide-induced release of cytokines, including tumor necrosis factor-α, interleukin (IL)-1, IL-6, and intercellular adhesion molecule-1 (p<0.05), and boosted NF-κB activation by elevating the expression of NF-κB p65, pIκBα, and IKKβ in neurons (p<0.05). Conclusion: Our results indicated that lncRNA ZFAS1 exacerbates epilepsy development by promoting neuronal apoptosis and inflammation, implying ZFAS1 as a promising treatment target for epilepsy.
Heping District, Taichung City, Central Taiwan D045&D046 Large-scale Landslide Area Investigation
( Chuan Ding ),( Ho-hsun Chen ),( Shu-ken Ho ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
The survey area, Songbo settlement, is located at Lishan area, Heping District, Taichung City near Techi Reservoir watershed, central Taiwan. There are many geomorphological features related to landslide and were divided into two large-scale landslide areas numbered as D045 and D046 by Central Geological Survey, MOEA. D046 has higher landslide-potential than D045. In order to ensure the operation of the Techi Reservoir and the safety of the Songbo settlement, the Taichung Branch of the Soil and Water Conservation Bureau promoted the project and carried out investigation and planning work. The stratum that produced the displacement at that time was explained to be the BIMROCK layer and the stratum is relatively stable. The unstable stratum is distributed in the colluvium (CV) and its thickness from a few meters to more than 50 meters. Through the field survey results including surface seepage points investigating, road cracks and subsidence sketching, gully and drainage ditch conditions investigating, construction, poles and trees investigating, and cracks in retaining walls sketching, etc., only local area shows higher landslide potential but whole area. Besides, monitoring data such as inclinometers casing, GPS, groundwater level observation and field survey results can identify the anomalies found in the activity of potential landslide bodies.