Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

음곡 상응부위에 시술한 방기 약침이 LPS로 유도된 흰쥐의 염증성 신손상에 미치는 영향

김기춘 ( Ki Choon Kim ),임윤경 ( Yun Kyoung Yim ) 경락경혈학회 2013 Korean Journal of Acupuncture Vol.30 No.4

Objectives: This study aimed to evaluate the effects of Stephaniae tetrandrae Radix Herbal-acupuncture (SR-HA) at KI10 (Umgok) on nephritis induced by lipopolysaccharide (LPS) in rats. Methods: Male SD rats (n=32) were allocated to four groups; normal, LPS, saline, and SR-HA. The rats in the LPS, saline, and SR-HA groups were injected intra-peritoneally with LPS (2 mg/kg) to induce nephritis. The rats in the saline and SR-HA groups were treated with saline injection and SR-HA respectively at KI10 three times a week. To evaluate the effects of SR-HA at KI10 on nephritis in rats, the authors evaluated the numbers of WBC and neutrophils in blood, and the levels of creatinine, TNF-α, CINC-1 in serum, creatinine, total protein in urine and renal MPO. Results: SR-HA at KI10 significantly inhibited the increase of WBC and neutrophils in blood, TNF-α, CINC-1 in serum, creatine, total protein in urine and MPO in kidney of LPS-stimulated rats. Conclusions: SR-HA at KI10 may have therapeutic effects on nephritis in LPS-stimulated rats.

한국 근대 자유시의 미적 전략과 근대적 서정의 형식

노춘기(Noh, Choon-Ki) 한국시학회 2015 한국시학연구 Vol.- No.44

The purpose of this article is investigation of the formation and the esthetic attitude of Korean modern free-verse. In 1900’s the poem written in Korean language was recognized as an effective means of enlightenment campaign. But in the respect of subject consciousness and linguistic form, the writers of free-verse showed a different intention point from the senior writers. Since the emergence of the magazine of HakJiKwang, the younger writers presented the pioneering realization of the modern literature and the artistic expression. The modern free-verse in Korean appeared for the first time in the middle of 1910’s. And Hwang, Seok-Woo declared the aesthetic theory of the free-verse as a form of modern poetry in late 1910’s. He insisted that the starting point of Korean modern poetry must be in the free-verse. It meant that the free-verse is the essence of modern poetry. And he stipulated that the word of soul-nonusual and unpopular expression-is the essentials of poetic language. He denied the form of presentation of the previous pattern of poetry as a chantable lyrics. The writers of the freeverse advocated the realistic portrayal of the torturous world and the wounded subject. They defended the modern free-verse and suggested the aesthetic goal for the future of Korean modern poetry.

만성 호중구성 백혈병 1례

기장도,박치영,류보열,김태종,모성환,곽재정,문철호,박근홍,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

Chronic neutrophilic leukemia is a very rare myeloproliferative disorder which is characterized by severe sustained mature neutrophilic leukocytosis in peripheral blood (PB), hepatosplenomegaly, elevated leukocyte alkaline phosphatase, serum uric acid. serun viatmin B 12 and the bleeding tendency despite normal platelet count and coagulation time, absence of philadelphia chromosome, and the absence of fever or underlying infection or disease sufficient to mimic a leukemoid reaction. We have experienced a 34-years-old male patient with atypical chronic neutrophilic leukemia. On admission, the leukocyte count was 56,860/uL. severe neutrophic leukocytosis with 76% neutrophils and rare immature forms on PB smear. Many neutrophils had toxic granules, and vacuoloes. Leukocyte alkaline phosphatase score was decreased. The cytogenetic study showed Philadelphia chromosome negative with normal karyotype.

도시소각회의 재활용 기술로서 내부발열소결법의 적용성

나춘기,정일현 木浦大學校 應用科學硏究院 2001 應用科學硏究誌 Vol.1 No.-

This paper describes the use of municipal solid waste incinerator (MSWI) ashes to manufacture synthetic aggregates using an innovative sintering technique, which was named "inner exothermic sintering (IES) method", characterized by co-treatment of sewage sludge as an inner heating source. The results of sintering experiments indicate that the added sewage sludge has powerfully acted as an inner heating source and a bloating agent in the sintering process of pellet, and thus could be manufacture of a double layered MSWI ash aggregate with fusion-bloated inner part and nonfusion dense outer surface. Futhermore, in this process, the fly ash also be co-treated without any particular change of the sintering condition. The synthetic aggregates are tested according to US regulation and fulfill regulatory limits, therefore, confirming their safe applications, although the results show some higher leaching from sintered aggregates than from melted slags. An overall conclusion from this is that the IES method represents an environment friendly and a solution for stabilising and converting MSWI ashes and sewage sludges into useful products.

우리 憲法上 大統領 彈劾制度에 관한 小考

宋基春 한국공법학회 2004 공법연구 Vol.32 No.5

The National Assembly passed motions for the impeachment of President Roh Moo-hyun on March 12, 2004 for election law violations, responsibility for the corruptions by his aides and alleged incompetence, and the Korean Constitutional made a decision to dismiss the impeachment motion on May 14, 2004. Under current laws, the National Assembly may pass motions for the impeachment of a president only when the president have violated the Constitution or other Acts in the performance of his or her official duties. In case a motion for impeachment against a president has been passed, the president shall be suspended from exercising his or her power until the impeachment has been adjudicated. The power of institution of impeachment is delegated to the National Assembly to check on the other branches. The effect of the resolution of the National Assembly to pass motions for the impeachment is different from that of the other countries in that the president is suspended from exercising his or her power until the impeachment has been adjudicated. There are some controversial issues in the interpretation of the conditions and proceedings of the impeachment against the President. The constitutional language of "in the performance" of "dfficial duties"(Article 65 Section 1) is ambiguous. Whether the National Assembly may pass the motion only when the violations of the Constitution and laws are clear and serious, what is the constitutional limit of the power to institute impeachment against the President, and whether it is possible for the National Assembly to withdraw the impeachment before the decision of the Constitutional Court, for example, are controversial. Another point of conflict is that there are no regulations to punish those who refuse to follow the court's order to take the witness stand. This thesis aims to clarify the ambiguous meaning of the provisions, and to solve the controversial issues of impeachment in Korean Constitutional Law.

여고생의 건강상태와 체력·성적에 관한 연구

민춘기,김동건 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.1

In the present study we employed the CMI to analyse the health status data for the 111 students from the C Girls High School in Daejeon City. The following results have been obtained as regards body strength and academic scores of the students in each group on mental health. 1. Of the students tested, 61.3% were classified into the group Ⅰand Ⅱ(normal and near normal respectively) and the remaining 38.7% into the group Ⅲ and Ⅳ(near neurosis and neurosis respectively). 2. The differences in body strength and academic scores of the students among the various groups were not significant.

점토벽돌제조용 원료로서 석탄회의 재활용성

나춘기,이무성,홍순강 木浦大學校 工業技術硏究所 1997 工業技術硏究誌 Vol.7 No.-

The purpose of this study is to examine the reusability of fly ashs produced by coal-fired power plants as an raw material in the manufacturing process of building resources such as brick, by investigating, in first, the mineralogical and chemical properties of raw materials including fly ashs of the Honam and the Samcheonpo thermal power plants and clay of the Hwangdeung area, and then examining the quility of pilot productions of fly ash-clay brick having various mixing proportions of fly ash. In general, the coal fly ashs separated in grain size under 200mesh and of Honam power plant have higher alkali contents but lower unburned carbon residues than those over 200mesh and of Samcheonpo power plant. This higher contents of alkali components such as CaO and Na2O and lower contents of unburned carbon have contributed to make dense structure in fly ahs-clay brick manufactured. Therefore, the clay brick manufactured by mixture of the Honam fly ash have more dence structure and much higher compressional strength than that of the Samcheonpo, and shows the trend of increasing the compressional strength from 1100-1300kg/㎠ to 170kg/㎠ with increasing the proportion of fly ash part from 10% to 50%. In contrast to Honam fly ash, the compressional strength of clay brick produced by the Samcheonpo fly ash decrease from 600-720kg/㎠ to 270-300kg/㎠ with increasing the mixing ratio of fly ash from 10% to 50%, due to high unburned carbon contents and low alkali contents of Samcheonpo fly ash.

성화의 교육적 가능성과 수단에 관한 연구

한춘기 總神大學校 1998 總神大論叢 Vol.17 No.-

<三韓拾遺>의 豫備的 考察

李春基 한양대학교 한국학연구소 1992 韓國學論集 Vol.20 No.-

This paper is only a part of the general study of the novel 'Samhan Seup-yu'(三韓拾遺). The purpose of this paper is to examine the original texts of the novel, current study on the novel, the family lineage and personal life of the author Kim So-haeng(金紹行) and the motive and process of his writing the book. Study on these subjects is believed to be of great help to understanding the novel 'Samhan Seup-yu' and its author. The conclusion of this study are as follows. Firstly, so far there has not been enough study on 'Samhan seup-yu'. There should be more study on the novel. Secondly, the extant texts of the novel can be classified into there at large; Seoul National University library original, Kora University library original and Karam Library original edition. But there are not any noticeable differance between them. Thirdly, the autheor Kim so-haeng was born in the influential Ahandong Kim family in the Choson Dynasty. but as he was a illegitimate child, he was restrained from getting any position in the government. So he devoted himself to learning and writting as a scholar and writer all his life. Lastly, the motive of his writing 'Samhan Seup-yu' can be traced back to his determined historical view and self-compensation phychology for his limited social status.