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      • 수지근에서 Neural Communication 의 전기생리학적 연구

        이상암,김광국,이명종 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.2

        It is well known that variations of the innervation in intrinsic hand muscles are common, A detailed anatomic study revealed that Riche -Cannieu anastomosis, a neutral anastomosis between the recurrent branch of the median nerve and the deep branch of the ulnar nerve, was in 77% of cadaveric dissections. Few electrophysiologic study of such anastomosis, however, has been done in a large population. The first group studied consisted of 88 limbs of 44 unselected subjects without Martin-Gruber anastomosis(MGA). The compound muscle action potentials(CMAP) were recorded by surface electrodes placed over the abductor pollicis brevis(APB), first dorsal interosseous(FDI), and abductor digiti quinti(ADQ) muscles with the stimulation at the wrist. The innervation of FDI by the median nerve was detected in 81(92.1%) of 88 limbs, and the innervation of APB by the ulnar nerve in 77(87.5% ). In no case, innervation of ADQ by the median nerve was found. The mean median nerve innervation ratio(MNIR) of FDI was 8.8%, and mean ulnar nerve innervation ratio(UNIR) of APB 23.5%. The second group, 37 MGA -detected limbs of 27 subjects, was also studied in order to evaluate the contribution of MGA to the innervation in intrinsic hand muscles. There was no significant difference in the incidence of anomalous innervation of each muscle in both groups.

      • Variation of Transition State in Reaction of Naphtalenesulfonyl Chlorides with Anilines in MeOH-MeCN Solvents

        KOO, In-sun,LEE, Chong-kwnag 慶尙大學校 1985 論文集 Vol.24 No.2

        1- 및 2-염화나프탈렌술포닐과 아닐린과의 친핵성 치환반응의 속도론적 연구를 MeOH-MeCN 혼합용매계에서 수행하였다. 본 연구에서 다음과 같은 결론을 얻었다. (ⅰ) 반응속도상수는 메탄올 100~90% 일 때 가장 컸고 친핵체의 치환기가 전자주계 친환기 일수록 증가하였다. (ⅱ) 1-NSC에서 S_N2형 전이상태에서 인접기 수소효과로 인한 입체장애 효과가 나타났다. (ⅲ) ρ값과 β값의 크기로 보아 전이상태에서 결합형성이 우세한 반응임을 알았다. (ⅳ) 생성물 닮은 전이상태를 수반하는 S_N2반응 메카니즘으로 진행됨을 알았다.

      • SCIESCOPUSKCI등재

        재조합 베타갈락토시다제 - 프리 S2 융합단백질에 존재하는 두 개의 메치오닌 위치에 올리고뉴클레오티드를 이용한 변이유도

        박종광,이상철,최영철,한문희,유명희 ( Chong Kwnag Park,Sang Chul Lee,Young Chul Choi,Moon H . Han,Myeong Hee Yu ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

        E. coli JM109 cells harboring pCTHB20 plasmid overproduced β-galactosidase-preS2 (βgal-preS2) fusion proteins with a yield of 50% of total cellular proteins (Choi et al., 1988). In order to facilitate the isolation of preS2 peptide out of the fusion protein after CNBr cleavage, the lacZ sequence was rearranged to construct pCTHB30 plasmid. Two internal methionine codons in the lacZ region of pCTHB20 and pCTHB30 was also substituted with valine codons by oligonucleotide-directed mutagenesis. The EcoRI-XbaI restriction fragment of lacZ was isolated from the plasmid pCTHB20 and was inserted into multicloning site of M13 mp18 vector. Two synthetic complementary oligonucleotides (17-mer each) with desired nucleotide changes were used simultaneously as primers in synthesizing a complementary strand. Mutant clones were screened by DNA hybridization with probes of ^(32)P-labeled mutagenic oligonucleotides, and the nucleotide substitutions were confirmed by DNA sequencing. E. coli JM109 cells transformed with pCMHB20 and pCMHB30 in which the lacZ regions were substituted with the mutant counterpart produced βgal-preS2 fusion proteins as efficiently as those with pCTHB20 and pCTHB30.

      • SCOPUSKCI등재

        N-Acetyl ${\alpha}$-aminoacrylic Acid의 자유라디칼 중합 및 혼성중합

        박일현,이종광,최재호,진정일,Il Hyun Park,Chong Kwnag Lee,Jae Ho Choi,Jung-Il Jin 대한화학회 1982 대한화학회지 Vol.26 No.4

        본 연구에서는 자유라디칼 메카니즘에 의한 N-acetyl ${\alpha}$-aminoacrylic acid의 단독중합 반응속도와 혼성중합 반응성을 연구하였으며, DMF 용매를 사용하여 $60^{\circ}C$에서 단독중합 시켰을 때의 중합속도 ($R_p$)는 단위체 농도 [M]와 개시제 농도 [I]에 대하여 $R_p$ = $k_p[M]^{0.97}[I]^{0.59}$의 관계를 나타내었다. 또한 이 단위체의 단독중합 총괄 활성화 에너지는 25.2 kcal/mole이었다. N-acetyl ${\alpha}$-aminoacrylic acid를 아크릴산 및 스티렌 단위체와 혼성중합 시켰을 때의 단위체 반응성비는 아래와 같았다. $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.49, $r_2$(acrylic acid) = 1.41; $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.44, $r_2$(styrene) = 0.91. Alfrey-Price 식을 이용하여 계산한 N-acetyl ${\alpha}$-aminoacrylic acid의 Q와 e값은 두 경우 모두 Q=0.51, e=0.16이었다. 단독중합체 및 혼성중합체들의 열적성질을 DTA 및 TGA법으로 분석하여 비교하여 보았다. The free radical polymerization and copolymerization of N-acetyl ${\alpha}$-aminoacrylic acid were investigated. From the result of kinetic investigation of N-acetyl ${\alpha}$-aminoacrylic acid in DMF at $60^{\circ}C$, a rate equation of $R_p$ = $k_p[M]^{0.97}[I]^{0.59}$ was obtained. The overall activation energy for the polymerization was found to be 25.2 kcal/mole. Copolymerization of N-acetyl ${\alpha}$-aminoacrylic acid with acrylic acid and styrene was carried out for the determination of monomer reactivity ratios. The monomer reactivity ratios for the monomer pairs determined at 70.0{\pm}0.1^{\circ}C$ using benzoyl peroxide as an initiator are; $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.49, $r_2$(acrylic acid) = 1.41, $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.44, $r_2$(styrene) = 0.91. The values of Alfrey-Price's Q and e parameters for N-acetyl ${\alpha}$-aminoacrylic acid were calculated to be 0.51 and 0.16 for the both systems. Differential thermal analysis and thermogravimetry showed that acrylic acid copolymers have poorer thermal stability as compared with the homopolymer of N-acetyl ${\alpha}$-aminoacrylic acid.

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