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Whole-genome resequencing analysis of 20 Micro-pigs
Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.
백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구
김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2
In this study, we tested the influences of several κ opioid ligands on the [<sup>3</sup>H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [<sup>3</sup>H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [<sup>3</sup>H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [<sup>3</sup>H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [<sup>3</sup>H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ<sub>1</sub>, and κ<sub>2</sub> receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [<sup>3</sup>H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ<sub>1</sub>, and κ<sub>2</sub>, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ<sub>2</sub> site which is dominant subtype of K receptor in rat cortex.
( Sang-Cheol Bae ),( Jin-Hye Cha ),( Jung-Yoon Choe ),( Sung Jae Choi ),( Soo-Kyung Cho ),( Won-Tae Chung ),( Chung-Il Joung ),( Young-Ok Jung ),( Young Mo Kang ),( Dong-Wook Kim ),( Jinseok Kim ),( Y 대한류마티스학회 2018 대한류마티스학회지 Vol.25 No.2
Objective. Productivity loss was compared by 3-stage of disease activity and associations between higher disease activity and high productivity loss were identified. Methods. Data were extracted from Rheumatoid Arthritis (RA) Patient-reported Outcomes Research, which enrolled 2,000 RA patients (>20-year) on disease-modifying-antirheumatic-drugs (DMARDs) (≥ 6-month) from December 2012 to June 2013. This included 1,457 RA patients with the disease activity score (DAS-28-ESR) in their medical charts. Productivity loss in time and indirect cost was estimated using The World Health Organization Health and Work Performance Questionnaire (HPQ). Baseline characteristics and productivity loss outcomes were compared according to DAS-28-ESR groups. Results. 84.4% were females, 54.2% had low DAS-28-ESR (<3.2), and 38.2% and 7.6% had moderate (3.2∼5.1) and high DAS-28-ESR (>5.1). Patients with moderate to high DAS-28-ESR had higher lost productivity time (LPT) and monthly costs of LPT than those with low DAS-28-ESR (time in hours: 110.0±58.4 vs. 132.4±57.2 vs. 71.5±52.0, p<0.0001; monthly costs of LPT in 1,000 Korean won: 1,097±607 vs. 1,302±554 vs. 741±531, p<0.0001). Multiple regression analyses revealed significant associations with high LPT in high (adjusted odds ratio [OR]=3.87, 95% confidence interval [CI]: 2.18∼6.87) and moderate DAS-28-ESR (adjusted OR=1.88, 95% CI: 1.41∼2.52) compared to low DAS-28-ESR. In addition, positive associations with high monthly costs of LPT were observed in high (adjusted OR=3.45, 95% CI: 1.98∼5.99) and moderate DAS-28-ESR (adjusted OR=1.93, 95% CI: 1.43∼2.54) compared to low DAS-28-ESR. Conclusion. Timely therapeutic strategies should be taken into consideration given that the RA patients with moderate to high DAS-28-ESR showed strong associations with high productivity loss for effective management of RA. (J Rheum Dis 2018;25:122-130)
Characterization of effector memory CD8+ T cells in the synovial fluid of rheumatoid arthritis.
Cho, Bon-A,Sim, Ji Hyun,Park, Ji Ah,Kim, Hye Won,Yoo, Wan-Hee,Lee, Seung-Hyun,Lee, Dong-Sup,Kang, Jae Seung,Hwang, Young-Il,Lee, Wang Jae,Kang, Insoo,Lee, Eun Bong,Kim, Hang-Rae Springer 2012 Journal of clinical immunology Vol.32 No.4
<P>Little is known about the cellular characteristics of CD8(+) T cells in rheumatoid arthritis (RA). We addressed this by investigating whether the frequency of the CD8(+) T cell subsets and their phenotypic characteristics are altered in the peripheral blood and synovial fluid (SF) from patients with RA. In this study, CD8(+) T cells, mainly CD45RA(-) effector memory (EM) CD8(+) T cells, were increased significantly in the SF, but not in the peripheral blood from RA patients, compared with healthy controls. The synovial EM CD8(+) T cells were activated phenotypes with high levels of CD80, CD86, and PD-1, and had a proliferating signature in vivo upon Ki-67 staining, whereas the Fas-positive cells were prone to apoptosis. In addition, EM CD8(+) T cells in the SF were less cytotoxic, as they expressed less perforin and granzyme B. In particular, the proportions of synovial fluid mononuclear cells that were CCR4(+)CD8(+) T cells and IL-4-producing CD8(+) T cells (i.e., Tc2 cells) were significantly higher than those in peripheral blood mononuclear cells of patients with RA and healthy controls. In addition, the number of IL-10-producing CD8(+) suppressor T (Ts) cells increased significantly in the SF of RA patients. Especially, CD8(+) T cells were inversely correlated with disease activity. These findings strongly suggest that EM CD8(+) T cells in the SF are increased, likely because of inflammation, and they may be involved in modulating inflammation, thereby affecting the development and progression of RA.</P>
HCV : PE-098 ; Myasthenia gravis triggered by antiviral therapy for chronic hepatitis C
( Hye In Kim ),( Tae Hun Kim ),( Il Hwan Moon ),( Kwon Yoo,),( Sun Young Yi ),( Sung Ae Jung ),( Ki Nam Shim ),( Hye Kyung Jung ),( Seong Eun Kim ),( Won Yung Cho ),( So Yoon Yoon ),( Eun Mi Song ),( 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Myasthenia gravis (MG) is an uncommon disease characterized by muscular weakness and fatigability, caused in 85% of the cases by Acetylcholine receptor (AChR) antibodies. And it is reported as a rare side effect of interferon administration which can develop during chronic hepatitis C treatment. And about 15% of MG patients have thymoma while half of the patients with thymoma eventually develop MG. But it is hardly reported that MG was triggered by antiviral therapy in patient with thymoma. We experienced a rare case of myasthenia gravis induced by antiviral therapy for chronic hepatitis C in patient with mediastinal thymoma. A 47-year-old male complained of diplopia after 9 weeks of administration of peginterferon and ribavirin. A brain imaging study showed no abnormality but we diagnosed as MG by positive repetitive nerve stimulation test and positive anti AChR antibody. And we found thymoma after chest CT scan.
Won-Ho Ha,Jae-Hyun Ahn,A-Ram You,Ji-Hye Kim,Min-Jeong Cho,Seung-Chul Shin 대한예방치과학회 2013 International Journal of Clinical Preventive Denti Vol.9 No.4
Objective: The purpose of this study was to investigate the stain removal, calculus deposit inhibition, and cleaning effect for teeth surface and interdental region by using toothpaste containing Pyrophosphate and soft granule (Zeolite-M). Methods: 1) For the study, general toothpaste was prepared as control and 2 kinds of test toothpastes were prepared. Test toothpaste-1(T1) containing Pyrophosphate and test toothpaste-2 (T2) containing Pyrophosphate and soft granule (Zeolite-M, Ultra White BallⓇ). 2) In-vitro study: The stain removal and interdental cleaning effect were measured using stained Hydroxyapatite tablet, and 20 subjects’ teeth surface and interdental cleaning effect by brushing was evaluated with test toothpaste. 3) Clinical study: The study was performed on 88 subjects. Calculus index and Stain index were measured during 8 week. Results: 1) T2 showed the highest stain removal and cleaning effect for teeth surface and interdental region. The results of the experiment were statistically significant (p<0.05). 2) After using toothpastes 4 weeks, subjects using T2 showed less tartar deposit than using control, and after 8 weeks, T2 statistically inhibit tartar formation than T1 and control (p<0.05). 3) Using T2 longer than 4 weeks could be accepted as a highly effective way to stain removal. Conclusion: T2 showed significantly effective on plaque, stain removal, calculus deposit inhibition and cleaning for teeth surface and interdental region than control toothpastes. Therefore, toothpaste containing pyrophosphate and soft granule is recommendable to prevent calculus formation and teeth staining. The subjects using T 2 can feel whitening efficacy and satisfaction of cleaning more than placebo and T1.
Hye-Won Jang,Seongman Bae,Youngmin Ko,Seong Jun Lim,Hye Eun Kwon,Joo Hee Jung,Hae yon Cho,Heounjeong Go,Hyun Wook Kwon,Young Hoon Kim,Sung-Han Kim,Sung Shin 대한이식학회 2021 Korean Journal of Transplantation Vol.35 No.4
The impact of the coronavirus disease 2019 (COVID-19) vaccination on humoral and cellular immunity in transplant recipients remains unknown. We report the case of a 78-year-old kidney transplant recipient who experienced acute T cell-mediated rejection after receiving the second dose of the BNT162b2 mRNA COVID-19 vaccine (Pfizer-BioNTech). She had no history of acute rejection throughout the 13 years after deceased donor kidney transplantation. Fifteen days after receiving the second dose of the BNT162b2 vaccine, the recipient visited our center with a mild headache and fever. Her serum creatinine level had increased from 0.61 to 4.95 mg/dL. Kidney allograft biopsy indicated acute T cell-mediated rejection (grade IB) with no pathologic evidence of antibody-mediated rejection. Anti-severe acute respiratory syndrome coronavirus 2 spike-immunoglobulin G and -immunoglobulin M measurements were weak positive and negative, respectively. Careful monitoring of kidney allograft function is vital for transplant recipients undergoing COVID-19 vaccination.