Alcohol extract of Geum Chung Cho alleviates alcohol-induced short-term memory impairment in gerbils

Han-Sam Cho,Tae-Woon Kim,Sang-Seo Park,Mal-Soon Shin,Jae-Min Lee,Eun-Sang Ji,Chang-Ju Kim 한국운동재활학회 2015 한국운동재활학회 학술대회 Vol.2015 No.10

Chronic ethanol consumption leads to memory impairment and also induces brain damage. The hippocampus has been particularly regarded as a possible target organ of alcoholic intoxication, since this structure plays a prominent function in memory processes. Cordyceps has been used as a traditional medicine in Asian countries for a long time. Different types of Cordyceps extract were reported to have various pharmacological activities including anti-tumor, anti-inflammatory, anti-viral, and immune modulatory effects. Guem Chung Cho is an artificial cultivated Cordyceps, and was supplied from GCordy (Seoul, Korea). Ethanol extract of Cordycep (50 g) was made by rotary evaporate, and resulting powder 8.29 g was obtained (yield 17%). In the present study, we investigated the effects of ethanol extract of Geum Chung Cho on short-term memory, cell proliferation and apoptosis in the hippocampus of ethanol-treated gerbils. The gerbils were divided into 5 groups (n = 10 in each group): Control group, alcohol-treated group, alcohol-treated and 0.001 mg/kg Geum Chung Cho-applied group, alcohol-treated and 0.01 mg/kg Geum Chung Cho-applied group, and alcohol-treated and 0.1 mg/kg Geum Chung Cho-applied group. Gerbils in the alcohol-treated groups were orally administered ethanol (1 g/kg) once a day for 2 weeks. Gerbils in the Guem Chung Cho-applied groups were orally administered Guem Chung Cho extract at each dosage 1 hour before ethanol consumption. Our results revealed that consumption of alcohol for 2 weeks deteriorated short-term memory, induced apoptosis, and inhibited cell proliferation, and reduced expressions of brain-derived neurotrophic (BDNF) and tyrosine kinase B (TrkB). However, Guem Chung Cho treatment alleviated alcohol-induced short-term memory impairment through enhancing cell proliferation and BDNF expression with inhibiting apoptosis in the hippocampus. Guem Chung Cho showed maximum effects at 0.1 mg/kg. Based on these results, alcohol extract of Guem Chung Cho may provide therapeutic value for the treatment of chronic alcohol-inducted brain injury. This study was supported by GCordy company.

수요지향 교과과정 개발을 위한 3차원 기반의 메타모델 설계 기법

조은숙,박수희,장준호 한국컴퓨터교육학회 2005 컴퓨터교육학회 논문지 Vol.8 No.6

KS-62 균주에 의한 펄프 표백 폐액처리에 관한 연구

조준형,은주영 江原大學校 附設 環境硏究所 2000 環境硏究 Vol.17 No.-

High colored Kraft bleaching effluent is one of the main constrains in pulp and paper indus-try due to its dissloved lignin derivatives. The degradation of lignin in pulp and paper mill effluent is mainly caused by white-rot fungi. This paper showed that the treatment with KS-62 fungus significantly reduced the color and chemical oxygen demand in the effluent. The amounts of Mn ions in the wastewater would play roles in the induction and activity of MnP (managanese peroxidase). Extracellular MnP was isolated from the fungus KS-62. The treat-ment with the MnP had the most effective decolorization in the wastewater treatment using nutrients medium.

교내 자판기와 터치스크린에 분포하는 세균의 실태조사

조주영,박창은 남서울대학교 2012 남서울대학교 논문집 Vol.18 No.1

The distributed species and the kinds of viable microorganism in button or touch screen of machine was monitored according to coffee & soda machine and automated teller machine(ATM). Our purpose was to investigate the distribution of bacteria in button or touch screen of machine. Analysis of dispenser contamination was sampled from 8 location in Namseoul University for 3 months from September to November in 2011. The isolated bacteria were identified by Gram stain and biochemical test using VITEKⅡsystems. The experiment on microorganism concentration of contact parts was carried out and the average of total microorganism was measured. The Coffee & soda machine and ATM was isolated and identified. The isolation rates of Gram positive cocci, Gram negative bacilli were 40.1%, 26.9% respectively. The frequently isolated bacteria were S. epidermidis, S. haemolyticus, S. warneri, Micrococus luteus/ lylae, Sphinogomonas paucimobilis . Further systematic studies are necessary with an emphasis on species identification Key Words : Microorganism, Coffee & soda machine, ATM

Aromatic Toxicants에 의해 유도된 간기능 장해에 미치는 쑥의 영향

조경열,손경선,윤수홍,박은주 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

간장해 물질인 phenylbutazone, aniline, benzo(a)pyrene을 이용하여 유도한 rats의 간독성발현에 미치는 쑥 추출액의 효과와 간장해 예방 효과를 관찰하여 다음과 같은 결과를 얻었다. 1. 성분 분석 결과 8종의 무기성분이 검출되었으떠 특히 potassium의 함량이 가장 높았고magnessium과 sodium의 함량이 높았다. 2. 총 14증의 지방산이 확인되었으며 그중 필수지방산인 linoleic acid와 linolenic acid의 함량이높았다. 3. 아피노산 분석 결과 총 16종이 검출되었으며 특히 aspartic acid, glutamic acid와 같은 산성아미노산의 함량이 높았다. 4. 쑥 추출액의 전처리는 phenylbutazone의 투여로 증가한 혈청과 조직중의 AST, ALT, ALP함량 증가를 현저히 회복신켰으나 혈청 LDH의 활성과 bilirubin 함량에는 효과를 나타내지않았다. 5. Aniline의 투여로 증가한 혈청과 간장중의 AST, ALT, LDH, ALP 활성을 쑥 추출액의 투여로크게 감소시킬 수 있었다. 그러나, bilirubin 함량에는 큰 변화가 없었다. 6. 쑥 추출액을 투여함으로씨 benzo(a)pyrene의 독성으로 증가한 혈청과 조직중의 AST, ALT,LDH, ALP 활성을 크게 감소시킬 수 있었으며 특히 LDH의 활성 회복 효과가 현저했다. In search for new drugs and methods of pharmacotherapy of liverdamage, we measured the chemical compositions of mugwort. And the mugwortextract was administrated to the experimental animal and investigated its bioche-mical effects in the serum and liver when aromatic hepatotoxicants - phenylbuta-zone, aniline and benzo(a)pyrene- was administrated. As the results are as follows : 1. 14 kinds of fatty acids,16 amino acids and 8 kinds of minerals were quantita-tively analyzed at mugwort. And major components of them were linoleic acid,aspartic acid, potassium and calcium. 2. The increase of serum and liver aminotransferase activity and bilirubin con-tent in hepatotoxicants treated group was markedly decreased by mugwortpretreatment.

HIV-1 Non-Nucleoside 역전사 효소 억제제의 생물학적 활성과 3차원 구조사이의 정량적 상관관계(3D-QSAR) 연구

조효영,윤은진,윤창주 가톨릭대학교 자연과학연구소 2003 자연과학논문집 Vol.24 No.-

본 연구에서는 AIDS라고 잘 알려진 후천성 면역 결핍(부전)증후군(Acquired Immuno Deficiency Syndrome)의 치료제로 사용될 수 있는 NNRTIs(Non-nucleoside Reverse Transcriptase Inhibitors)에 대해, 3D-QSAR방법으로 이들의 약리단(phrmacophore model)을 정의하고자 한다. NNRTIs는 HIV의 유전물질이 건강한 세포로 옮겨 갈 수 없도록 역 전사효소에 달라붙어 DNA합성을 방해함으로써 HIV의 증식을 막는다. 이들의 대표적인 화합물들은 HEPT(Hydroxyethoxymethylphenylthiothymine), TIBO(tetrahydroimidazo-benzodiazepinone), Nevirapine 등으로, 이들이 이번 연구에 사용된 화합물들이다. 지금까지의 연구에서는 이들의 결합 모형을 각각 연구 해 왔지만 X-ray 구조를 보면 NNRTI들이 역 전사 효소의 결합부위와 상호 작용할 때 유사한 입체구조를 갖는다고 할 수 있다. 따라서 이번 연구에서는 이러한 사실을 확인하기 위해 3D-QSAR 방법을 이용하여 NNRTIs 각각의 약리성 자리와 3차원 입체구조를 X-ray 구조와 비교, 분석하였다. 마지막으로, 이렇게 해서 얻은 활성가설을 하나의 '분자 체'로 이용하여 National Cancer Institute(NCI)의 AIDS관련 화합물들로 이루어진 데이터 베이스를 검색함으로써 선도물질이 될 수 있는 구조들을 검색해 보았다. A definition of pharmacophore for the NNRTIs(Non-Nuceoside Reverse Transcripase Inhibitors) was carried by considering a 3D-QSAR model. HEFT (hydroxyethoxy -methylphenylthiothymine), TIBO(tetrahydroimidazobenzodiazepinone) and Nevirapine among NNRTIs were used in this study. Pharmacophore models generated for the NNRTI binding pocket have been usually limited so far to a single class of compound. But NNRTIs have all been shown by X-ray crystallography to share a common binding location. Then, these inhibitors seem general similar conformation when bound to the pocket of RT. It has been confirmed that the similar interactions between the three class inhibitors have been shown by superimposing of pharmacophore models. The 3D-pharmacophore models as molecule sieve was applied in NCI database in order to find the potent inhibitors. The results provided a good basis to design new inhibitors of HIV-1 RT.

고비중 Bupivacaine과 Tetracaine에 의한 척추마취의 비교

조남수,유병식,박은주 최신의학사 1989 最新醫學 Vol.32 No.11

莞島治岸海域의 海洋環境特性

曺炷煥,姜恩淑 朝鮮大學校 自然科學硏究所 1995 自然科學硏究 Vol.18 No.1

An investigation on the physical and chemical conditions of the sea water off Wando Island was carried out during a period from February to November, 1992, with the following results. Water temperature ranged from 6.03 to 24.52 ℃. The inner sea water temperature was lower than the outer sea water by as much as 1 ℃ in winter, but higher by as much as 1~3 ℃ in summer. Salinity ranged from 29.70 to 34.95 ‰, with the higher figure occuring in April, and the lower occuhng in August, when the salinity in the inner sea was at its lowest on account of the influx of rainfall. pH ranged from 8.05 to 8.46, lowest in winter, the average was 8.20. Dissolved oxygen content ranged from 3.91 to 7.03 ㎖/ℓ, the higher occuring in winter, and the lower occuhng in summer when the water temperature was highest. COD ranged from 0.23 to 2.51 ㎎/ℓ, with the inner sea water lower than the outer sea water except in winter. Suspended solids were in the range of 2.5-41.5 ㎎/ℓ, the higher occuring in winter, the lower occuring in fall. Average distribution in the inner sea water was higher than the outer except winter. Transparency ranged from 0.5 to 6.1 m, the lower occuring in winter, the higher occuring in summer. The relation between suspended solids and transparency was S=26.012-6.439T (r=0.813) in the outer sea water. The phosphate concentration ranged from 0.05 to 0.48 ㎲-at/ℓ, the higher occuring in winter, the lower occuring in summer with a wide variation. The dissolved inorganic nitrogen (DIN) ranged from 0.71 to 6.29 ㎲-at/ℓ, higher in the bottom and the outer sea water than in the surface and the inner sea water. From the above investigation the sea area showed a wide variation with time and place or season. These factors are very important for the inhabitance and laying eggs of aquatic life. The sea adjacent the coast of Wando is comparatively pure as yet. But recently, on account of the increasing pollution by materials flowing from inland, the environment of aquatic life gradually worsening. From the data collected in this research it is possible to conclude that 'A countermeasure for the preservation of oceanic environment' with a purpose of decreasing the level of pollution materials which affects the ocean is necessary.

교사 학습공동체 내 지식창출 활동의 성격 및 촉진요인에 대한 사례 연구

주영주,조은아 이화여자대학교 사범대학 교과교육연구소 2006 교과교육학연구 Vol.10 No.1

지식기반사회를 맞아 지식과 학습의 관계에 대한 다양한 논의가 이루어지고 있는데, 그 중 사람들간의 협력적 관계나 실제 활동을 통해 생산되는 역동적 지식의 창출과 공유를 위해 현재 활발하게 시도되고 있는 학습방법 및 지식관리전략 중 하나가 학습공동체라 할 수 있다. 기업의 지식경영 영역에서 뿐만 아니라, 교육현장에서의 새로운 전문성과 경쟁력을 제고하는 방법으로서도 학습공동체를 통한 지식창출이 중요한 이슈가 되고 있다. 학습공동체에 대한 논의는 이미 교육현장에서부터 시작되어 왔으나, 그 실행에 있어 교육청의 행정적 관여로 인해 교사들의 자발적 참여가 확신되지 못했고, 교사들의 요구를 충족시키지 못했다는 문제점들이 있었다. 이에 본 연구는 문화관광부의 지원대상이 되며 문화예술분야의 교사들을 대상으로 구축된 학습공동체인 '교사자율연구모임'의 사례를 통해, 교사 학습공동체의 유형과 공동체 내에서 이루어지는 지식창출 활동의 성격을 분석하고, 교사의 지식창출 환경으로서의 학습공동체 촉진요인을 도출해 보고자 하였다. '교사자율연구모임'을 분석한 결과, 동 학습공동체는 동일 분야의 동료 교사들과 상호협력적 학습을 통해 지식을 공유함으로써 교사로서의 자신의 전문성을 개발하는 것이 주요 목적인 전문성을 위한 학습 공동체로 파악되었다. 또한 학습공동체들은 대부분 연구와 현장활동을 병행하고 있었다. 교사자율연구 모임 내에서 습득, 공유되는 지식과 경험은 주로 교육콘텐츠의 제작 및 창작 활동, 연구 및 이론 개발, 공동의 문제해결을 위한 학습으로서의 연수활동 등이었다. 교사자율연구모임을 활성화시키기 위해 공동체로서의 목표공유, 참여에 대한 자발성, 동료의식 등 사회적 요인 등이 주요 촉진 요인으로 도출되었다. 특히 공동체 의식, 동료의식 등 정의적 영역의 요인들이 교과연구를 통한 인지적 성과달성에 비해 실제 교사 학습공동체의 지식창출 및 공유활동을 촉진시키는 요인으로 도출되었다. Learning Community is the learning method and knowledge management strategy which is actively attempted of collaborative learning and creation and sharing of knowledge being produced in authentic context. Knowledge creation through Learning Community has become an important issue not only in the fi디d knowledge management, but also in the educational field. Many studies on Learning Community have already begun in the field of education. But, there has been some obstacles to voluntary participation of teachers due to administrative intervention of educational offices. Accordingly, this study tries to analyze the type of teachers' Learning Community and the characteristics of knowledge-creating activities occurring in Learning Community, and to discover promotive elements of Learning Community as teachers' knowledge-creating environment, by example of "Teachers' Voluntary Research Group", which is a Learning Community aimed at teachers in the field of arts and culture, supported by the Ministry of Culture and Tourism. After analyzing the "Teachers' Voluntary Research Group", I concluded; the group was considered as a Learning Community aimed at developing their competency as teachers, by sharing knowledge through collaborative learning with colleagues belonged to the same field. Also, most of communities conducted researched and practices at the same time. The main knowledge and experience being acquired and shared in the "Teachers' Voluntary Research Group" was production of educational contents, research and developing theory and collaborative learning for problem-solving. Major promotive elements of the "Teachers' Voluntary Research Group" were social elements such as joint enterprise of community, voluntary participation and sense of colleagues. Especially, the sense of community and the sense of colleagues were indicated as more effective elements to promote knowledge creation and sharing of teachers' learning community, rather than cognitive ones.

바터 팽대부에 생긴 유암종 1예

강명주,지삼룡,박석주,이재익,조영완,김준영,박성재,박은택,이연재,이상혁,설상영,배상균 인제대학교 2006 仁濟醫學 Vol.27 No.-

Carcinoid tumors have been reported in a wide range of organs but most commonly involve the lungs, bronchi and gastrointestinal tract. Within the gastrointestinal tract, appendix is the most common location for carcinoid, followed by the distal small intestine, the rectum, and the stomach. Among these, primary involvement of the ampulla of Vater is extremely rare. We report a case of carcinoid tumor of ampulla of Vater. A 62-year-old man presented with epigastric soreness. Gastroduodenal endoscopy showed hyperemic bulging mass on ampulla of Vater and diagnosed as carcinoid tumor by histologic finding, with immunohistochemical study. In ^(111)In-octreoscan, tumor had regional lymph node metastasis, but no evidence of distant metastasis. Pancreatoduodenectomy with lymph node dissection was performed.