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윤대환 ( Dae Hwan Youn ),박찬규 ( Chna Kyu Park ),김정상 ( Jeong Sang Kim ),채우석 ( Woo Seok Chae ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1
Objectives: This research was performed to investigate the effect of acupuncture at the KI2, KI10, HT8, HT3 on Aquaporin-2(AQP2) expression related with the renal functions in rats. Methods: Acupuncture was performed during 100-seconds, 6-times, at 150-seconds intervals under anesthesia in rats. We observed rats` mean arterial pressure(MAP), heart rate(HR), renal sympathetic nerve activity(RSNA) during acupuncture and AQP2 expression by western blot method and atrial natriuretic peptide(ANP), renin, norepinephrine of plasma after decapitation. Results: The AQP2 expression was significantly increased in HT8 group, but decreased in KI10 group. Average MAP during 6-times acupuncture was significantly increased in HT8 group. Average HR was significantly increased in HT8 group, Average RSNA was increased in KI10 group, but that was marginally increased in KI10 group. Plasma renin concentration was increased in KI2 , HT3 groups. Plasma ANP show a tendency to decrease in KI10, HT3 groups, increased in KI2 , HT8 but not significant. Plasma norepinephrine concentration was significantly decreased in KI10, HT3 groups. Conclusions: These results suggested that acupuncture at HT8 activate renal function to reuptake, but KI10 show a decline on effect of AQP2 expression, blood pressure, nerve activity and renin.