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Antonio Montes,Chandrasekar Chinnarasu,Clara Pereyra,Lourdes Casas,María Teresa Fernández-Ponce,Casimiro Mantell,Sangma Pattabhi,Enrique Martínez de la Ossa 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2
Various extracts from olive leaves have been precipitated by a supercritical antisolvent (SAS) process to evaluate the possibility of producing polyphenol fine particles with controlled size and size distribution. Olive leaves were initially extracted with subcritical fluids using mixtures of CO2+ethanol at 10% and 50%, by pressurized liquid extraction (PLE) with water, ethanol and a hydroalcoholic mixture (50 : 50) (v/v), and also by conventional ethanol extraction (CE). PLE gave the extract with the highest yield and the best antioxidant activity. SAS precipitation was unsuccessful for the extracts obtained with pressurized water and with the hydroalcoholic mixture (50 : 50) (v/v). The SAS precipitates with the smallest particle sizes were produced from extracts obtained with subcritical fluids. The SAS precipitates obtained after the conventional ethanol extraction of olive leaves showed the best antioxidant activity.