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Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.
Chi,Hyung-Joon,Yoon,Jae-Ho,Yang,Deok-Chun,Song,Won-Seob 한국자원식물학회 2003 Plant Resources Vol.6 No.3
The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.
흰쥐 적출간 관류법을 이용한 벤지딘계 염료 Direct Black 38의 대사
원종욱,노재훈,김춘성,박윤정,김치년 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.1
Direct Black 38, a kind of benzidine-based azo dye, is widely used as a dye for fabric, leather, cotton, cellulosic material, paper, wool, silk, and so on. Benzidine-based azo dyes are proven as a mutagen and linked to bladder cancer. In 1978, National Institute for Occupational safety and Health recommended that three widely used benzidine-based dyes(Direct Black 38, Direct Blue 6, and Direct Brown 95) should be treated as carcinogens. In this experiment, metabolism of the benzidine-based dye, Direct Black 38 was examined by using an isolated liver perfusion system. To measure the metbolites of Direct Black 38, 8.0μM, 30.5μM and 63,3μM of Direct Black 38 was added into the recirculating perfusate of the isolated perfused rat liver. Samples were collected at 0, 10, 20, 30, 60, 90, 120 minute. They were treated with sep-pak and methanol, and the metabolites were detected and quantified with high performance liquid chromatography(HPLC). Residual non-reactive dye in the perfusate and liver was reduced to benezidine and then analyzed by HPLC. Detected metabolites of Direct Black 38 were benzidine, N-acetybenzidine, and N,N'-diacetylbenzidine. The average concentration of benzidine was 0.1μM and this concentration was maintained throughout the experimental period. The average concentration of Nacetylbenzidine was 0.22μM and took the same pattern of benzidine. When 30.5μM of Direct Black 38 was added to the perfusate, only N,N'-diacetylbenzidine increased slightly with time. From the above results we suggest that only small amount of Dirst Black 38 might be metabolized to benzidine regardless of the s, amount of the Direct Black 38. There are some possible explanations. The liver was damaged during the perparation. And the function of the perfused liver decreased rapidly because adsorbing of dye. The benzidine, acetybezidine and diacetylbenzidine metabolized to other metabolites were not detected in this experiment.
원치문 한라대학교 2007 論文集 Vol.10 No.-
This paper presents the results of a test program for flexural strengthening characteristics of continuous unidirectional flexible carbon-fiber sheets(CFS) bonded or/and developed to reinforced concrete(RC) beams. A total of three 300 mm × 500 mm × 6,400 mm concrete beams were tested. Beam without any strengthening, beam bonded with CFS, and beam bonded and developed with CFS using development device were studied to determine their effects on flexural strength of the beams. From the tests, it was found that the strength increases with the development of sheets at development device. Among the three beams, beam bonded and developed with CFS using development device provided the most effective strengthening for concrete beam. Beam strengthened using this scheme showed 97.8% increase in flexural capacity as compared to the control beam without any strengthening.
위의 상피성 종양에서 p53 암억제 유전자 및 EB 바이러스 RNA의 발현에 관한 연구
원치규,이중달 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2
Background: Mutant p53 gene and Epstein-Barr virus genome (EBER) are expressed in adenocarcinoma cells of the stomach, and their biologic significance involving in gastric carcinogenesis is evaluated. Method: A variety of precancerous and cancerous lesions including intestinal metaplasia(three cases), dysplasia(four cases), adenoma(three cases) and adenocarcinoma(11 cases) were investigated in formalin-fixed, paraffin-embedded tissue sections. Immunohistochemical reaction using monoclonal antibody for p53 gene and in situ hybridization using anti-sense probe for EBER were studied. Results: One of three adenomas and two of 11 adenocarcinomas expressed the p53, and EBER were expressed in one of 11 adenocarcinomas. Conclusions: Relaiton between their overexpression and histologic type of gastric adenocarcinomas was not found. Since the p53 and EBER wer overexpressed in different cases, their cooperative action involving gastric carcinogenesis was believed to be not present.
Girder의 강성을 고려한 복합 재료 교량 상판의 구조 거동
원치문,박제선,이정호,심도식 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-
Many of the bidge and building floor systems, including the girders and cross-beams, also behave a similar special orthotropic plates. Such plates are subject to the concentrate masses in the form of traffic loads, or the test equipments such as the accelerator in addition to their own masses. Analysis of such problems is usually very difficult. Most of the bridge slabs on girders have large aspect ratios. Finite difference method is used for this purpose, in this paper. The reuslt is compared with that of the beam theory.
우리나라 시 · 군 · 구 지역보건의료계획의 비전(Vision) 문구 분석
안치영(Chi-Young Ahn),김현수(Hyun-Soo Kim),김원빈(Won-bin Kim),오창훈(Chang-hoon Oh),홍지영(Jee-Young Hong),김은영(Eun-Young Kim),이무식(Moo-Sik Lee) 한국농촌의학 지역보건학회 2017 농촌의학·지역보건 Vol.42 No.1
지역보건의료계획서에서 비전은 각 지역의 보건의료계획을 나타내는 핵심요소임에도 불구하고 작성방법에 대한 구체적인 지침이 부재하여 각 지역의 비전은 매우 다양하게 표현되어 있다. 이에 이 연구에서는 제6기 지역보건의료계획에서 제시한 비전 문구를 보건소 유형별, 권역별로 분석하였다. 전국 229개 지역의 제6기 지역보건의료계획서의 비전 문구와 이와 관련된 미션, 전략체계도, 중장기 추진과제 등을 대상으로 하였다. 지역을 권역별 4개, 읍·면·동 구성에 따른 보건소 유형을 4개로 분류하였고, 각 지역 비전의 글자 수, 문장 평가, 단어 빈도, 문구 평가를 빈도분석과 카이제곱검정, 일원배치분산분석을 이용하여 분석하였다. 비전의 글자 수가 17자 이하인 보건소가 172개소(75.1%)였고, 보건소 유형별로 유의한 차이가 있었다(p<0.05). 문구에 대한 수사표현에서 비유법(37.1%)이, 한글 외에 표현 언어 사용에서는 특수문자(43.2%)가 많았으며, 보건소 유형별로 유의한 차이가 있었다(p<0.01). 비전에 사용된 단어들은 ‘건강’, ‘행복’, ‘함께’, ‘군민’ 또는 ‘시민’, ‘도시’, ‘100세’ 등이었다. 문구평가 점수에서는 특·광역시 및 수도권에서 가장 높았으며, 보건소 유형 및 권역별로 유의한 차이를 보였다(p<0.05). 보건소 유형별로 과정 및 평가 영역, 공유가능성 등에서 유의한 차이가 있었다(p<0.01). 방향성은 충청권, 수도권 순으로 권역별로 유의한 차이가 있었으며(p<0.01), 미래지향성 및 공유가능성은 영남권이 가장 높아 권역별로 유의한 차이가 있었다(p<0.01). 비전은 지역보건의료계획에 가장 중요 요소 중 하나이다. 이 연구는 지역사회 특성별로 비전의 설정이 다름을 확인하였으며, 이러한 결과를 통하여 지역보건의료계획 등 보건소 비전 수립시 참고가 될 수 있는 기초적인 자료가 될 것으로 판단된다. Objectives: In this study, we analyzed vision statements of the 6th community health plan of local government in Korea. Methods: We examined vision statements letters, missions and strategy plans, and long-term missions of 6th community health plans of 229 local government in Korea. We also analyzed the numbers of vision letters, sentence examination, word frequency, each vision statement with frequency analysis, chi-square test, and one-way ANOVA. Results: Among 229 local government, 172(75.1%) of local government had the number of letters (Korean) less than 17 of vision statements, and there were a significant differences according to type of community health centers (p<0.05). Figuration (37.1%) were the most used in an expression of vision statement sentence, and special characters (43.2%) were the most used language except Korean. The most commonly used words of vision statement in order of frequency were ‘health’, ‘happiness’, ‘with’, ‘citizen’, ‘city’, ‘100 years old’ etc. Chungcheong provinces and Daejeon metropolitan city had a highest score in directionality on phrase evaluation, and there were a significant differences according to regional classes of local government (p<0.01). Gyeongsang provinces, Ulsan, Daegu, and Busan metropolitan cities had a highest score in future orientation and sharing possibilities on phrase evaluation, and there were a significant differences according to regional classes of local government (p<0.01). Conclusions: Vision is one of the most important component of community health plan. We need more detailed ‘vision statement guideline’ and the community health care centers of local government should effort to make more clear and complete their vision.
전자제어 스파크 점화 GIE의 연소 및 배기 특성에 관한 연구
김치원 慶南大學校 附設 工業技術硏究所 2003 硏究論文集 Vol.21 No.2
본문에서 스파크 점화 방식이며 흡기관 내 가솔린 다점 분사식인 4행정 사이클 엔진을 성능 시험장치로 설계 개조하였고, 퍼지제어기의 응답특성, 연소특성, 배기 배출물의 생성 그리고 엔진 성능특성 등을 엔진에 공급되는 당량비를 운전인자로 변화시키면서 시험하고 분석하였다. 등력발생장치에 공급되는 에너지 농도 즉 공기와 연료의 정밀도를 제어할 수 있도록 제어논리로부터 엔진동력의 부하와 속도에 따라 비례적분 이득을 결정하고, 요구되는 당량비를 제어하여 해석인자(열발생율, 질량연소율, 연소기간 및 점화지연기간, 배기생성)의 특성들을 규명하였다. In this paper, the response characteristics of fuzzy controller, combustion, emission formation and performance characteristics are analyzed with changing the equivalence ratio and engine load. The experimental phase of this investigation was performed and analyzed with 4-stroke, spark ignited, and gasoline injected engine which was modified for this experiment by fuzzy controlled injection system.
단기통 스파크 점화기관의 가솔린 분사제어 장치의 설계 및 성능 특성에 관한 연구
김치원,윤창식,김판호 경남대학교 환경문제연구소 1994 환경연구 Vol.16 No.-
최근 자동차용기관에 관한 연구 및 기술은 점차적으로 기관이 고속화되고 주행성의 쾌적, 유해배기가스의 규제강화, 제동연료 소비율의 향상을 동시에 만족하는 것이다. 따라서 기관의 압축비를 높이는 방법과 연료를 실린더내와 홉기관에 직적 분사시키는 방법이 활발히 연구 개발되고 있으며 대책의 일환으로 최적상태의 공연비로 분사시기와 분사량을 정확하게 제어함 수 있는 방법이 요구되고 있다. 본 연구에서는 분사시기와 분사량을 제어할 수 있는 전자제어 연료분사 장치를 설계 제작, 단기통 스파크 점화기관에 설치하여 기관의 연소 및 성능 특성을 고찰하였다. 실린더 압력경과 및 연소율은 연료분사시기 BTDC 50, 공연비 13:1 에서 향상 되었고 분사시기를 진각 또는 지각시키려면 HC배출량은 증가하였으나 CO의 배기농도는 큰 영향이 없었다. In rescent years, to improve the combustion characteristics of spark ignition engine has been studied about the electronic fuel injector instead of carburetor fuel supply. The experimental equipment of this study consisted of a circuit to detect injection timing and duration for investigating the effect of fuel injector. As the result of this analysis, the combustion characteristics and the hamful exhaust emission have been enhanced in the gasoline injection type SI engine
스파크점화 기관의 회전형 흡배기장치의 개발에 의한 체적효율 향상에 관한 연구
김치원,윤창식,김유식 경남대학교 신소재연구소 1995 論文集 Vol.5 No.-
최근 자동차용 기관의 연구에 있어서는 고성능, 고효율과가 추진되고 있으며, 본 연구에서도 기관의 체적효율 향상을 달성하기 위하여 회전밸브를 설계 제작하였으며 기존의 포핏밸브와 실험적 방법을 통하여 비교하였다. 본 연구의 결과 회전식 흡배기 밸브장치에서 유입유동 저항이 현저하게 낮아짐으로서 체적효율의 향상을 기할 수 있었으며, 따라서 자동차용 기관의 성능이 높게 나타남을 확인할 수 있었다. In recent years, the study on the high efficiency of the internal combustion engine has been mainly proceeding. In this study, we developed rotary valve to achieve the improvement of volumetric efficiency and to be simple construction. And then made a comparative analysis between rotary and poppet valve. In this experiment, rotary valve enlarged the flow area of valve port to minimize the resistance of the fluid flow and to flow smoothly in intake and exhaust process. Indeed, valve timing was controlled properly lest positive pressure in exhaust process should affect intake process. Motoring and firing experiments were using engine speed and air-fuel ratio a s the principle parameter and the full opening of throttle valve and minimum spark advance for best torque(MBT) as engine operating variables.