Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Extended use of Prostate Health Index and percentage of [-2]pro-prostate-specific antigen in Chinese men with prostate specific antigen 10–20 ng/mL and normal digital rectal examination

Peter Ka-Fung Chiu,Jeremy Yuen-Chun Teoh,Wai-Man Lee,Chi-Hang Yee,Eddie Shu-Yin Chan,See-Ming Hou,Chi-Fai Ng 대한비뇨의학회 2016 Investigative and Clinical Urology Vol.57 No.5

Purpose: We investigated the extended use of Prostate Health Index (PHI) and percentage of [-2]pro-prostate-specific antigen (%p2PSA) in Chinese men with prostate-specific antigen (PSA) 10–20 ng/mL and normal digital rectal examination (DRE). Materials and Methods: All consecutive Chinese men with PSA 10–20 ng/mL and normal DRE who agreed for transrectal ultrasound (TRUS)-guided 10-core prostate biopsy were recruited. Blood samples were taken immediately before TRUS-guided prostate biopsy. The performances of total PSA (tPSA), %free-to-total PSA (%fPSA), %p2PSA, and PHI were compared using logistic regression, receiver operating characteristic, and decision curve analyses (DCA). Results: From 2008 to 2015, 312 consecutive Chinese men were included. Among them, 53 out of 312 (17.0%) men were diagnosed to have prostate cancer on biopsy. The proportions of men with positive biopsies were 6.7% in PHI<35, 22.8% in PHI 35–55, and 54.5% in PHI>55 (chi-square test, p<0.001). The area under curves (AUC) of the base model including age, tPSA and status of initial/repeated biopsy was 0.64. Adding %p2PSA and PHI to the base model improved the AUC to 0.79 (p<0.001) and 0.78 (p<0.001), respectively, and provided net clinical benefit in DCA. The positive biopsy rates of Gleason 7 or above prostate cancers were 2.2% for PHI<35, 7.9% for PHI 35–55, and 36.4% for PHI>55 (chi-square test, p<0.001). By utilizing the PHI cutoff of 35 to men with PSA 10–20 ng/mL and normal DRE, 57.1% (178 of 312) biopsies could be avoided. Conclusions: Both PHI and %p2PSA performed well in predicting prostate cancer and high grade prostate cancer. The use of PHI and %p2PSA should be extended to Chinese men with PSA 10–20 ng/mL and normal DRE.

A Cross-Cultural Comparative Study of Cultural Characteristics and Informa tion Search Behaviours of Business Travellers

Che-Chao Chiang,Min-chi Chang,Po-Tsang Chen,Fu-Ming Chiang 세계문화관광학회 2010 Conference Proceedings Vol.11 No.0

This study examines the information searching of business travelers and their behavior based on the cultural differences. Despite growing academic interest in business travel i n recent years, there has been little investigation of the information search behaviors of business travelers in a cross-culture context. The above themes are rational assumed that information search behaviour are necessary to understand. To address the identified gap s in the literature, the proposed relationships are examined and presented through using chi-square method with a survey sample. The research sample included Japanese, English and Chinese business travellers. A self-administrated questionnaire was distributed to 211 business travelers attending international conventions in Taipei. The research findings reveal that information preferences of business travelers are significantly differed from the three recognized groups in the case of Taiwan. Some significant differences in information search behaviors were recognized amongst the three recognized respondent groups. Marketing implications were also proposed and presented.

물리치료사의 직무유형이 직무만족도와 조직몰입도에 미치는 영향 : 부산지역을 중심으로

김성구 ( Seong Gu Kim ),노정운 ( Jung Woon No ),박민규 ( Ming Yu Park ),하용목 ( Yong Mok Ha ),김지혁 ( Chi Hyok Kim ),배원식 ( Won Sik Bae ) 대한통합의학회 2014 대한통합의학회지 Vol.2 No.4

Purpose : The purpose of this survey was to investigate the effect on job satisfaction and organizational commitment according to the job types of Pusan area physical therapists figuring out structural causality between job satisfaction and organizational commitment. Method : This survey was conducted through structured questionnaire for the physical therapists working in Pusan area, and data from 217 therapists were utilized in the final analysis. Data collection was deployment questionnaire using Google Docs (online survey), The SPSS 21.0 program was used according to for research purposes independent t-test was used for individual and job characteristics, was used according to job types and job satisfaction and organizational commitment are logistic regression analysis, correlation analysis and chi-square test. Result : The results of this study were as follows. 1. Overall job satisfaction according to the job types was appeared musculoskeletal physical therapist more 4 times. 2. Job types according to the organizational commitment did not make direct effect. Conclusion : Number of musculoskeletal physical therapist than neurological physical therapist was fewer and there were many per day treatment of patients, but overall job satisfaction was appeared that is higher. This is thought that job types affects more than working environment.

ON V-RINGS, P-V-RINGS AND INJECTIVITY

Ming, Roger Yue Chi Department of Mathematics 1992 Kyungpook mathematical journal Vol.32 No.2

Predisposing Factors of Pressure Sore in Patients with Spinal Surgery

Ming Chi Sung,Chun Jung Peng,Ming Feng Kang 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

ON GENERALIZATIONS OF SELF-INJECTIVE AND STRONGLY REGULAR RINGS

Ming, Roger Yue Chi Department of Mathematics 1986 Kyungpook mathematical journal Vol.26 No.2

Isolation of triterpenic acid-rich extracts from Hedyotis corymbosa using ultrasound-assisted supercritical carbon dioxide extraction and determination of their fictitious solubilities

Ming-Chi Wei,Show-Jen Hong,Yu-Chiao Yang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

The present study investigated a green ultrasound-assisted supercritical carbon dioxide (USC–CO2)process for extracting oleanolic acid (OA) and ursolic acid (UA) from Hedyotis corymbosa. Chrastil, Bartleand Kumar and Johnston models were used to correlate the experimentalfictitious solubility data. Theoverall average absolute relative deviation (AARD (%)) in solid solubility ranged from 3.632% to 6.529%,which indicates good agreement between the experimentalfictitious solubility data and the modeledresults. Using the correlation results, the total heat of solution and the enthalpies of vaporization andsolvation for OA and UA in the USC–CO2 extraction process were also estimated.

Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells

Ming, Lim Chi,Halim, Murni,Rahim, Raha Abd,Wan, Ho Yin,Ariff, Arbakariya Bin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirredtank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBCs and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher ($10.7{\times}10^{10}CFU/mL$) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher ($11.3{\times}10^{12}CFU/g_{Glucose}$) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.

The effect of gait training with an elastic ankle-foot orthosis on balance and walking ability of persons with chronic stroke: a randomized controlled trial

Chi, Ming Hao,Yim, Jong Eun,Yi, Dong hyun korean Academy of Physical Therapy Rehabilitation 2020 Physical therapy rehabilitation science Vol.9 No.4

Objective: The purpose of this study was to investigate the effect of treadmill exercise on the posture and walking speed of chronic stroke survivors with an ankle-foot orthosis. Design: Randomized controlled trial. Methods: Twenty-four chronic persons with chronic stroke admitted to Bobath Memorial Hospital in Seongnam city were divided into two groups by random blind method. Treadmill exercise with an elastic ankle-foot orthosis was performed in the experimental group and treadmill exercise was performed in the control group. The experiment was carried out for 6 weeks, and the experiment was carried out three times a week for 20 minutes per session. To measure the effect, static balance was measured using the MTD system before and after training, and the Berg Balance Scale (BBS) was used to measure functional balance. Results: There was a statistically significant difference between the 2 groups in the BBS measurement results for confirming the functional balance (p<0.05). Also, there was a significant difference between the 2 groups in single limb support time, step time and step length (p<0.05). Conclusions: In this study, it was found that treadmill exercise with an elastic ankle-foot orthosis in persons with chronic stroke was effective in maintaining functional balance, walking ability, step length, and step time. Therefore, it is necessary to use a flexible ankle-foot orthosis with proper treadmill exercise as a method of improving balance and walking speed of chronic stroke survivors.