자궁경부암에서 혈관 내피 성장인자 ( VEGF ) 및 VEGF Mrna 의 발현에 대한 연구

이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi),염윤석(Yoon Seok Yum),이주희(Ju Hee Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : Angiogenesis, the formation of blood vessels by sprouting from pre-existing ones, is essential for the growth of solid tumors beyond 2~3mm in diameter and for tumor metastasis. Vascular endothelial growth factor (VEGF), is known as vascular permeability factor(VPF) and mediates vascularization and tumor-induced angiogenesis. This study examined the potential of growth, invasion, and metastasis of uterine cervical carcinomas associated with neovascularization. Methods : From January 1996 to December 1999, at the Department of Obstetrics and Gynecology, Kyung-Hee University Hospital, 37 uterine cervical carcinomas and 7 normal cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Immunohistochemical staining for VEGF was carried out to study VEGF localization, and the levels of VEGF subtype mRNAs were determined by quantitative RT-PCR in specimens. The relation between VEGF subtypes expression of cervical cancers was analysed. Results : The positive staining for VEGF is seen dominantly in the cytoplasm of the cancer cells, and faintly in interstitial cells. The intensity of staining was stronger in squamous carcinomas than in adenocrcinomas, but there was no significant difference (p>0.05). Quantitative RT-PCR analysis demonstrated significantly increased VEGF121/VEGF165 mRNA expression levels (>0.56/>0.72) in 21 (56.8%) and 15 (40.5%) of 37 cervical carcinomas comparing to control groups (mean: 0.28/0.36). There was no obvious relationship between VEGF121/VEGF165 mRNA expression levels and the clinical parameters examined including age, pathology, differentiation, tumor size, lymphovascular space invasion, LN involvement and invasion depth except clinical stage (p<0.05). Conclusions : The overexpression of VEGF mRNA may be an important contributing factor in cervical carcinomas. There is no significant differenece of VEGF mRNAs levels according to clinical parameters, so it seems that the expression of VEGF is involved in the promotion of angiogenesis on cervical cancer and plays an important role in early invasion.

실리카흄을 흔입한 고강도 콘크리트 개발

홍창우,김태경,김경진,김성환,김남윤,심도식,이정호,윤청호,백민경,원치문,박제선,이주형,정경일 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

콘크리트 구조물이 대형화됨에 따라 설계단면이 증대되어 상대적으로 많은 경제적 손실을 부담하게 된다. 따라서 단면을 감소시키면서도 소요의 하중에 안전한 구조물을 건설하기 위해서는 우선적으로 구조물 건설에 기초가 되는 고품질, 고내구성의 고강도 콘크리트 개발이 절실히 요구된다 본 연구에서는 일정 시멘트비 및 혼화재 비율하에서 소요의 워커빌리티가 확보되는 고강도 콘크리트를 시간과 온도의 변화에 따라 증기 양생하여, 압축, 인장, 휨강도 뿐만 아니라 파괴특성을 실험적으로 연구하였다. 시료 제작시 시멘트 입자 사이의 공극 및 불연속 영역을 충전하여 고밀도화하기 위한 콘크리트용 혼화재로 시멘트 비표면적이 상당히 작은 초미립 분말인 실리카흄을 이용하였다. 또한 AE감수제 및 고성능 유동화제를 사용하여 혼화재의 첨가에 의하여 발생될 수 있는 워커빌리티의 감소를 방지하였다. 실험결과에 의하면 일정 양생 지속 시간하에서 온도의 증가에 따라 콘크리트의 압축, 인장 및 휨강도가 전반적으로 증가하였다. 동일하게 일정 온도하에서 양생 지속시간이 커짐에 따라 강도들이 증가됨을 알 수 있었다. 따라서 상대적으로 높은 온도와 긴 지속 양생 조건하에서 파괴에 대한 저항력이 크게 나타났다. The compressible, tensible, and flexibleresistance of the high strength concrete is analyzed by the experimentation in the present study. For the test, we cure several samples with the silica fume as a mixture being become dense the spaces between the particle of cement under the variation of both the temperature and the curing-interval. Then, the superplasticizer and the ezcon are also used to satisfy the required workability for construction. The compressible, tensible, and flexible resistances to a stress are increased as increasing the temperature and the time interval for the curing. Therefore it is concluded that the overal fractural and mechanical properties is improved by mixing the silica fume into the cement.

알코올 의존과 후보 유전자들간의 연관성 연구

정인원,김헌,홍주봉,지경환,이규영 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.6

연구목적 : 알코올 대사와 중추신경계내의 알코올 작용부위와 관련한 일부 후보유전자들이 알코올 의존과 관련이 있는지를 확인하고자 하였다. 방 법 : DSM-IV로 진단된 알코올 의존 환자 128명과 정상 대조군 128명을 대상으로 후보 유전자들의 다형성을 중합효소 연쇄반응과 제한효소처리법을 이용하여 동정하였다. 여기서 분리된 대립유전자와 유전자형에 따른 빈도의 차이를 유전자별로 각각 비교하였으며, 알코올 의존에 작용하는 서로 다른 유전자들에 의한 영향력을 배제하기 위하여 logistic regression 분석을 적용하였다. 결 과 : 알코올 의존군에서는 ALDH_2 유전자의 NN(wild type)형이 정상대조군에 비하여 유의하게 많았다(chi-square test, p<0.001). logistic regression analysis를 통하여 다른 유전자들에 의한 영향을 보정한 상태에서 ALDH_2가 NN(wild type)인 경우 다른 아형에 비하여 알코올 의존에 대한 odds ratio는 130.312이며 그 신뢰구간은 (17.22, 986.43)이었다. 한편, ASA는 N-glycosylation 부위에 변이형 대립유전자를 갖고 있는 경우(AG or GG)는 wild type에 비하여 알코올 의존에 대한 위험도는 2.344배이며 그 95% 신뢰구간은 (1.128, 4.871)이었다. 결 론 : 알코올 의존과 ALDH_2 유전자간에는 밀접한 연관성이 확인되었다. 또한 다른 유전자들은 개별 연관분석에서 의미있는 연관성이 관찰되지는 않았지만 logistic regression analysis를 통하여 N-glycosylation 부위에 다형성이 있는 ASA 유전자가 알코올 의존과 관련이 있음을 시사하였다. Objectives : This study was to explore the association between alcohol dependence and five candidate genes related to the metabolism of alcohol and the enzymes of the suspected sites in CNS. Methods : The genotype and allele frequencies of five candidate genes in 128 male hospitalized patients who met DSM-IV criteria for alcohol dependence were compared with 128 age-matched healthy male control subjects using polymerase chain reaction and restriction fragment length polymorphism. A logistic regression analysis was applied in order to exclude the reciprocal interactions among five candidate genes. Results : The NN genotype frequency of the ALDH_2 gene was significantly higher in alcoholic patients than in control subjects(chi-square test, p<0.001). No difference in frequency was found in the other four genes. In a logistic regression analysis, the odds ratio for alcohol dependence in the NN genotype of the ALDH_2 gene and AG or GG genotypes of the N glycosylation site on the ASA gene were 130.312(95% confidence interval, 17.22-986.43) and 2.344(95% confidence interval. 1.128-4.871), respectively. Conclusion : The result reiterates the association of the ALDH_2 gene polyporphism and the alcohol dependence. Logistic regression analysis additionally suggested that the N-glycosylation site on the ASA gene was associated with alcohol dependence.

일 도시 여고생의 음주관련 요인에 관한 연구

김주형,김은경,김효정,문현숙,오선민,박나경,신혜정,이경원,이지선,최연옥,최진희,한유정,현수인 대한간호학회정신간호학회 2002 정신간호학회지 Vol.11 No.1

This study was designed to examine the factors associated with alcohol consumption among the Korean high schools girls. 283 female students were sampled from two high school in Seoul, Korea. They were given questionnaires and were asked to fill in and return them during a class hours. The factors included in the questionnaire were analyzed to see their contributions to alcohol consumption using chi-square. Alcohol consumption was associated with lower school performance(p=.001). High alcohol expectancies increased alcohol consumption(p=.000); however, depression did not show a statistically significant relationship to alcohol consumption. Mothers' alcohol consumption, having many alcohol consuming friends and watching TV commercials picturing alcohol were found to be associated with alcohol consumption, p=.049, .000, .000. respectively. Results may imply that alcohol consumption may not be a coping behavior of the depressed high school girls. Parents in Korea should be more concerned about alcohol consumption and its impacts on their daughters. Media effects on alcohol consumption need to be stressed and deserve attention from the public.

화상으로 인한 소구증 환자의 치험례

지재휴,이병준,김영균,김수관,황경곤,여환호,박인순 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.3

Deep burns of the face & lips often lead to scarring and contraction of the circum-oral tissues with a marked reduction in the size of the oral aperture. Such burns most commonly caused by electrical contact, for example, children sucking electric plugs, or as a result of chemical burns and the exposture to flame. Once having burns, burned tissue may contract and reduce considerably the size and mobility of the mouth ; therefore bring up the resultant functional disturbance, verbal difficulty, even disgestive difficulty, and poor appearance, which fall into difficulty in social acceptability, caused by burns. In our department, 2 patients who were complain of functional limitations and esthetic problem owing to scar contracture, were visited, and we treated this microstomia with scar excision, graft and flap technique, and postoperative intensive physical therapy. We obtained relatively favorable results, thus report this cases with literature review.

생검재료를 토대로 한 심재성 진균증

윤경애,지제근 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.4

미용성형수술 환자의 심리적 특성과 미용수술이 이에 미치는 영향

지경환,이상익 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

최근 일반인의 미용수술에 대한 인식이 달라지게 되면서, 많은 수의 일반인들이 미용성형 수술에 대해보다 긍정적인 태도를 가지게 되었다. 하지만 지금까지의 연구 결과는 미용성형 수술을 받은 환자의 경우 정신과적 이상이 있다는 보고가 많았다. 본 연구는 미용성형수술을 받은 환자군을 대상으로 다양한 심리학적 평가를 실시하여, ① 미용성형수술환자의 정신과적·심리적 특성을 밝혀낸 후 ② 재건수술군 및 일반 대조군과의 비교를 통해, 최근 미용성형수술을 원하는 환자들의 변화되었을 것으로 추정되는 성형과 ③ 미용성형수술이 이들에게 미치는 영향에 대해 조사하였다. 방 법 : 충북대학교병원 성형외과와 청주시내의 두 개인 성형외과를 방문하여 미용성형수술과 재건수술을 받은 환자군 50명을 대상으로 하였다. 각 집단의 심리적 특성을 알아보기 위해 기초 설문조사, 신체상 척도, 자아주체성, 간이정신진단검사 등의 측정도구로 수술 전과 수술 후의 심리상태를 측정하였다. 결 과 : 1) 재건수술 환자군이 미용수술군 및 대조군에 비해 신체적 문제로 인한 심리적 스트레스를 더 심하게 받고 있었다. 2) 신체상 척도, 자아주체성 척도, 간이정신진단검사에 미용수술군과 대조군 간의 유의한 차이는 없었다. 3) 미용수술군은 수술 후 신체화, 강박증, 대인예민성, 우울, 불안, 적대감, 편집증 GSI, PSDI, PST등 간이 정신진단검사의 거의 모든 항목에서 호전이 있었다. 4) 미용수술군에서 수술후 만족도에 영향을 미치는 요인으로 자아주체성 척도의 자기수용, 도특성 항목과 간이정신진담검사의 강박증, 신체와 척도가 있었다. 자기 수용이 낮고, 자신에 대한 독특성이 높을수록 만족도가 높았고, 신체적 증상 호소가 많을수록 만족도가 낮았다. 5) 미용수술군에서 수술전 기대감이 높을수록 만족도가 낮은 유의한 상관관계를 보였다. 결 론. 이상의 결과에서 미용성형수술은 원하는 환자들이 정신 병리적으로 일반인과 차이가 없었으며, 미용성형수술이 환자들의 정신건강을 호전시킬 수 있다는 것이 밝혀졌다. 또 신체적 증상 호소 정도와 자신의 건강에 대한 생각, 그리고 안전성과 강박성에 대한 평가를 할 수 있는 간편한 검사 방법이 개발된다면 수술 후 만족도가 낮거나 재수술을 반복할 경향이 있는 환자를 선별할 수 있는 좋은 도구가 될 것이다. Objectives : Along with the fact that people change their attitude on aesthetic surgery, many people become to have positive viewpoint on aesthetic surgery. However, according to the recent study result, it showed that aesthetic surgery patients tend to have psychopathology. To investigate effects of aesthetic surgery and tendency of aesthetic surgery patients which is presumed to be changed, various psychological evaluations were performed in the sample who had undergone aesthetic surgery. Also psychiatric and psychological attributes of aesthetic plastic surgery patients were compared with those of reconstructive surgery group and those of normal controls. Method : 50 of reconstructive surgery group and 50 of normal control group were selected from Dept. of Plastic Surgery of Chungbuk National University Hospital and two private plastic surgery clinics in Cheong-ju. To find out each group's psychological attributes, pre-and post-operative psychological conditions were evaluated by administering basic survey, body image scale, ego identity scale, and SCL-90-R. Result : 1) Patients in the group of reconstructive surgery received more stress than those in the group of aesthetic surgery and normal controls. 2) There were no significant differences in the scores of body image scale, ego identity scale and SCL-90-R between reconstructive surgery group and normal control group. 3) After aesthetic surgery, aesthetic surgery group showed improvement in almost every item in SCL-90-R, such as somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, paranoid ideation, GSI, PSDI and PST sub-scales. 4) The factors which affected on the satisfaction after surgery among group of aesthetic surgery were self-acceptance, uniqueness in self-identity scale and obsessive-compulsive, somatization in SCL-90-R. When self-acceptance was low and self-uniqueness was high, satisfaction was high. And the more patients complained about physical symptom, satisfaction was low. 5) The factors which influenced on the opinion of re-operation among group of aesthetic surgery were stability in self-identity scale, obsessive-compulsive and somatization in SCL-90-R, and physical health in body image scale. When stability was low, complaints about physical wymptoms were high, viewpoint on one's health was negative and the chance of re-operation was high. 6) In group of aesthetic surgery, the higher the expectation was before surgery, the lower the satisfaction was after surgery. Conclusion : As described in above study result, patients who planned to have aesthetic surgery were not different from common people in terms of psychopathology. And it was also found that aesthetic surgery could improve patient's mental health. If simply administrable evaluation method to measure complaints about physical symptoms, viewpoint on one's health, stability and obsessive-compulsive is invented in the near future, it will be possible to screen patients who are likely to have low satisfaction after surgery and high tendency to have re-operation.

Epstein-Barr virus(EBV)와 연관된 Nasal type T/Natural Killer(NK)-cell lymphoma 1예

지두현,최지호,성경제,문기찬,고재경 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

Nasal type T/NK-cell lymphoma는 병리조직학적으로 응고성 괴사가 동반된 혈관중심성 침윤을 특징으로하며 T-cell과 NK-cell 항원을 모두 표현하는 면역조직화학염색 소견을 보이는 매우 드문 피부 림프종으로서 EBV와의 연관성이 흔히 보고되고있다. 저자들은 42세 여자에서 발생한, 전신적인 홍반성 구진, 판, 결절의 임상양상을 보였으며 피부 병변에서의 EBV-encoded RNA(EBER)에 대한 in situ hybridization상 양성소견을 보였고 전산화단층촬영, 골주사, 갈륨주사 등의 검사상 비강, 위장관 및 다른 내부장기 침범소견은 보이지 않아서 피부에만 국한된 경우로 사료되는 nasal type T/NK-cell lymphoma 1예를 보고하는 바이다.

Ciprofloxacin의 동물유래 병원세균에 대한 시험관내 항균효과시험

장경수,장치훈,김일택,박승춘,윤효인,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In this study the antimicrobial spectrum and sensitivity of ciprofloxacin(CFX) against the major pathogenic bacteria isolated from the diseased poultry, pig, cattle in Korea were evaluated in comparison with morfloxacin(NFX), enrofloxacin(EFX), nalidixic acid(NA), gentamicin(GM), tetracycline(TC), erythromycin(EM), streptomycin(SM) and penicillin(PC). Increasing by paper disk diffusion test for total of 439 isolates from poultry, pig and cattle, CFX showed remarkably higher sensitivity(>83%) as compared with other drugs. When three quinolones such as CFX, NFX and NA were compared for the inhibition activity against 4 major pathogens, CFX induced significantly larger diameter of inhibition zone through all of the tested concentration than NFX and NA. As MICs of all drugs for 11 bacterial species isolated from poultry were measured, the MIC range of CFX was 0.125 - 40 ㎍/㎖ in Gram positives, ≤ 0.005 - 2.5 ㎍/㎖ in Gram negatives and 0.08 - 1.25 ㎍/㎖ with 12 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX in these cases revealed consistently lower as compared with those of NFX, GM and PC. When the MICs of the drugs were tested with 11 bacterial species from the diseased pigs, MIC range of CFX was 0.05 - 3.5 ㎍/㎖ in Gram positives, ≤ 0.005 - 0.8 ㎍/㎖ with Gram negatives and 0.5 - 9.0 ㎍/㎖ with 11 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX and NFX were remarkably lower than those of GM and PC. As MICs of CFX, NFX, GM and PC for 10 bacterial species isolated from cattle were tested, the MIC range of CFX was found 0.01 - 4.0 ㎍/㎖ in Gram positives, and ≤ 0.005 - 0.2 ㎍/㎖ in Gram negative bacteria. The MIC_50 and MIC_90 of CFX in cases of cattle appeared remarkably lower than those of the other drugs. CFX, as compared with the other drugs, showed invariably lower MIC_50 and MIC_90 in both of GRam positives and negatives. As MICs of CFX, NFX and PC for 12 reference bacteria were examined, MIC of CFX was 0.03 - 0.25 ㎍/㎖ that was much lower drug concentration than those of NFX(0.25 - 1.0 ㎍/㎖) and PC(0.5 - 16.0㎍/㎖).

Evolutionary Programming for Designing Independent Cells

문치웅,김장형,이유경 濟州大學校 産業技術硏究所 1997 산업기술연구소논문집 Vol.8 No.1

In this paper, an evolutionary programming approach is proposed for the designing independent cells in cellular manufacturing with alternative process plans and machine duplication consideration. Several manufacturing parameters such as production volume, machine capacity, processing time, number of cells and cell size are considered in the process. The model is formulated as a 0-1 integer programming and solved using genetic algorithm. It determines the machine cells, part families and process plan for each part simultaneously.